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1. 发明申请

WO2009158658A3 INDUCTION OF FLOCCULATION IN PHOTOSYNTHETIC ORGANISMS 审中-公开
标题翻译：诱导光合作用中的絮凝
公开(公告)号：WO2009158658A3
公开(公告)日：2010-04-29
申请号：PCT/US2009048929
申请日：2009-06-26
申请人： SAPPHIRE ENERGY INC , MENDEZ MICHAEL , BEHNKE CRAIG , POON YAN , LEE PHILIP
发明人： MENDEZ MICHAEL , BEHNKE CRAIG , POON YAN , LEE PHILIP
IPC分类号： C12N1/13 , C07K16/14 , C12N15/63
CPC分类号： C12N1/12 , C07K14/4726 , C07K16/16 , C12N1/02
摘要： The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.
摘要翻译：本发明提供了用于在光合生物体中产生絮凝部分的组合物和方法。光合生物被遗传修饰以影响絮凝部分的产生，分泌或两者。还提供了絮凝生物体的方法。

2. 发明申请

WO2010019813A2 PRODUCTION OF FATTY ACTDS BY GENETICALLY MODIFIED PHOTOSYNTHETIC ORGANISMS 审中-公开
标题翻译：基因改造光合生物体的脂肪酸生产
公开(公告)号：WO2010019813A2
公开(公告)日：2010-02-18
申请号：PCT/US2009053772
申请日：2009-08-13
申请人： SAPPHIRE ENERGY INC , MENDEZ MICHAEL , O'NEILL BRYAN , BURKART MICHAEL , BEHNKE CRAIG , LIEBERMAN SOYAN , BIELINSKI VINCE , POON YAN
发明人： MENDEZ MICHAEL , O'NEILL BRYAN , BURKART MICHAEL , BEHNKE CRAIG , LIEBERMAN SOYAN , BIELINSKI VINCE , POON YAN
IPC分类号： C12P7/64 , C12N1/21 , C12N15/52 , C12N15/63
CPC分类号： C12N9/16 , C12N9/0006 , C12N9/0083 , C12N9/1029 , C12N9/90 , C12N9/93 , C12P7/26 , C12P7/6409 , C12P7/6463
摘要： The present invention relates to compositions that facilitate the production of enzymes by genetically modified photosynthetic microorganisms wherein the enzymes contribute to the synthesis of fatty acids, lipids or oils. In some embodiments the compositions are one or more vectors that encode one or more polypeptides that increase production of fatty acids, lipids or oils. The vectors may comprise promoters and other nucleic acid sequences configured for expression of the polypeptides in the chloroplast of non-vascular, photosynthetic organisms. In some embodiments the compositions are genetically altered photosynthetic microorgansims or genetically altered chloroplasts. The present invention provides methods of producing one or more proteins, including fatty acid synthesizing enzymes in a photosynthetic microorganism. In some embodiments the present invention is a method of preparing a fuel. The methods disclosed herein allow for the production of crude or refined biofuels, including ethanol.
摘要翻译：本发明涉及促进通过遗传修饰的光合微生物生产酶的组合物，其中所述酶有助于脂肪酸，脂质或油的合成。在一些实施方案中，组合物是编码增加脂肪酸，脂质或油的产生的一种或多种多肽的一种或多种载体。载体可以包含启动子和其他核酸序列，其被配置用于在非血管光合生物的叶绿体中表达多肽。在一些实施方案中，组合物是遗传改变的光合微生物或遗传改变的叶绿体。本发明提供了生产一种或多种蛋白质的方法，所述蛋白质包括光合微生物中的脂肪酸合成酶。在一些实施例中，本发明是一种制备燃料的方法。本文公开的方法允许生产粗制或精制的生物燃料，包括乙醇。

3. 发明公开

EP2531600A4 STRESS-INDUCED LIPID TRIGGER 审中-公开
标题翻译： STRESSINDUZIERTERLIPIDAUSLÖSER
公开(公告)号：EP2531600A4
公开(公告)日：2013-08-07
申请号：EP11740278
申请日：2011-02-01
申请人： SAPPHIRE ENERGY INC
发明人： YOHN CHRISTOPHER , MENDEZ MICHAEL , BEHNKE CRAIG , BRAND APRIL
IPC分类号： C12N15/29 , C12P7/64
CPC分类号： C12P7/6463 , C07K14/405 , C12N15/8247 , C12P7/6409

4. 发明公开

EP2381761A4 GENETICALLY ENGINEERED HERBICIDE RESISTANT ALGAE 审中-公开
标题翻译： GM除草剂耐藻类
公开(公告)号：EP2381761A4
公开(公告)日：2012-06-20
申请号：EP09837013
申请日：2009-12-22
申请人： SAPPHIRE ENERGY INC
发明人： FANG SU-CHIUNG , POON YAN , MENDEZ MICHAEL
IPC分类号： A01H13/00 , C12N1/13 , C12N5/04 , C12N15/03 , C12N15/05 , C12N15/29
CPC分类号： C12N15/79 , C12N9/0004 , C12N9/1022 , C12N9/1092 , C12N15/8274 , C12N15/8275 , C12N15/8278

5. 发明公开

EP2477478A4 A system for transformation of the chloroplast genome of Scenedesmus sp. and Dunaliella sp. 有权
标题翻译：系统，用于栅藻转化叶绿体基因组SP。和盐藻。
公开(公告)号：EP2477478A4
公开(公告)日：2013-02-13
申请号：EP10817726
申请日：2010-09-14
申请人： SAPPHIRE ENERGY INC
发明人： BOTSCH KYLE , SZYJKA SHAWN , LEVINE WENDY , CURRAN AMY , O'NEILL BRYAN , MENDEZ MICHAEL
IPC分类号： C12N9/24 , A01H13/00 , C12N1/13 , C12N15/79 , C12N15/82
CPC分类号： C12N15/8207 , C12N9/242 , C12N15/79 , C12N15/8209 , C12N15/8214

6. 发明专利

AU2015203662A1 Induction of flocculation in photosynthetic organisms 未知
公开(公告)号：AU2015203662A1
公开(公告)日：2015-07-23
申请号：AU2015203662
申请日：2015-06-30
申请人： SAPPHIRE ENERGY INC
发明人： MENDEZ MICHAEL , BEHNKE CRAIG , POON YAN , LEE PHILIP
IPC分类号： C12N1/13 , C07K16/14 , C12N15/63
摘要： The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.

7. 发明专利

CA2692893C HIGH THROUGHPUT SCREENING OF GENETICALLY MODIFIED PHOTOSYNTHETIC ORGANISMS 未知
公开(公告)号：CA2692893C
公开(公告)日：2015-06-23
申请号：CA2692893
申请日：2008-05-30
申请人： SCRIPPS RESEARCH INST , SAPPHIRE ENERGY INC
发明人： MAYFIELD STEPHEN , O'NEILL BRYAN , MENDEZ MICHAEL , MIKKELSON KARI , POON YAN
IPC分类号： C12Q1/68 , C12M1/00
摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

8. 发明专利

GB2475435B System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts 未知
公开(公告)号：GB2475435B
公开(公告)日：2012-03-28
申请号：GB201100928
申请日：2008-10-06
申请人： SAPPHIRE ENERGY INC
发明人： MENDEZ MICHAEL , O'NEILL BRYAN , MIKKELSON KARI
IPC分类号： C12N15/82 , C12N15/79 , C12N15/81

9. 发明专利

GB2475435A Producing a product using shuttle vectors containing essential chloroplast genes 未知
公开(公告)号：GB2475435A
公开(公告)日：2011-05-18
申请号：GB201100928
申请日：2008-10-06
申请人： SAPPHIRE ENERGY INC
发明人： MENDEZ MICHAEL , O'NEILL BRYAN , MIKKELSON KARI
IPC分类号： C12N15/82 , C12N15/63 , C12N15/68 , C12N15/74 , C12N15/81
摘要： The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. A method of producing a product is provided, comprising obtaining a plurality of vectors collectively providing all chloroplast genes necessary for photosynthesis, each vector having two homologous recombination regions, one vector having a homologous polynucleotide of interest, one vector having a yeast replication element, one having a bacterial replication element, and one having a yeast stability element/selection marker and not having the yeast replication element; assembling the vectors into an assembled genome by introducing into yeast, selecting for assembly using the stability element/selection marker, introducing the assembled chloroplast genome into a photosynthetic host organism and expressing the homologous polynucleotide to produce the product of interest. The product may be a terpene, terpenoid, fatty acid or biomass degrading enzyme. The host may be an algae or cyanobacterium. The bacterial replication element may be a P1 or F factor replication element. The yeast replication element may be a yeast centromere or autonomous replication sequence. The yeast selection marker may be URA3 and an additional marker.

10. 发明专利

AU2009302889B2 System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts 未知
公开(公告)号：AU2009302889B2
公开(公告)日：2015-01-22
申请号：AU2009302889
申请日：2009-04-08
申请人： SAPPHIRE ENERGY INC
发明人： MENDEZ MICHAEL , O'NEILL BRYAN , MIKKELSON KARI
IPC分类号： C12N15/63 , A01H1/00 , C12N15/82 , C12Q1/68
摘要： The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast- bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes.

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