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1. 发明申请

US20110293779A1 BARLEY AND MALT-DERIVED BEVERAGES WITH LOW DMS LEVEL 有权
标题翻译： BARLEY和MALT衍生的饮料低DMS水平
公开(公告)号：US20110293779A1
公开(公告)日：2011-12-01
申请号：US13132765
申请日：2009-12-01
申请人： Søren Knudsen , Gustav Hambraeus , Lene Molskov Bech , Steen Bech Sorensen , Birgitte Skadhauge , Klaus Breddam , Ole Olsen
发明人： Søren Knudsen , Gustav Hambraeus , Lene Molskov Bech , Steen Bech Sorensen , Birgitte Skadhauge , Klaus Breddam , Ole Olsen
IPC分类号： A01H5/00 , C12C1/18 , C12C7/01 , A01H1/06 , C12C1/027
CPC分类号： C12N9/1007 , C12C1/18 , C12C12/00 , C12C2200/01 , C12N15/8243
摘要： According to the invention, there is provided barley-derived beverages characterized by notably reduced levels of both dimethyl sulfide (DMS) and/or its precursor S-methyl-L-methionine (SMM), or lacking said compounds. In addition, the invention relates to methods for producing the above-mentioned beverages—and also to barley plants useful in the preparation of such beverages, as well as other plant products prepared from said plants. Utilization of the invention clears the way for making improved production procedures of beverages with improved taste profiles, and promises also for notable reductions in the thermal energy input for production of beer.
摘要翻译：根据本发明，提供了大麦衍生的饮料，其特征在于二甲硫醚（DMS）和/或其前体S-甲基-L-甲硫氨酸（SMM）的水平显着降低，或缺乏所述化合物。此外，本发明涉及用于制备上述饮料的方法以及用于制备这种饮料的大麦植物以及从所述植物制备的其它植物产品。本发明的利用清除了具有改进的味道特征的饮料改进生产程序的方法，并且还有助于显着降低生产啤酒的热能输入。

2. 发明授权

US09834760B2 Barley and malt-derived beverages with low DMS level 有权
公开(公告)号：US09834760B2
公开(公告)日：2017-12-05
申请号：US13132765
申请日：2009-12-01
申请人： Søren Knudsen , Gustav Hambraeus , Lene Molskov Bech , Steen Bech Sørensen , Birgitte Skadhauge , Klaus Breddam , Ole Olsen
发明人： Søren Knudsen , Gustav Hambraeus , Lene Molskov Bech , Steen Bech Sørensen , Birgitte Skadhauge , Klaus Breddam , Ole Olsen
IPC分类号： A01H5/00 , C12N9/10 , C12C1/18 , C12C12/00 , C12N15/82
CPC分类号： C12N9/1007 , C12C1/18 , C12C12/00 , C12C2200/01 , C12N15/8243
摘要： According to the invention, there is provided barley-derived beverages characterized by notably reduced levels of both dimethyl sulfide (DMS) and/or its precursor S-methyl-L-methionine (SMM), or lacking said compounds. In addition, the invention relates to methods for producing the above-mentioned beverages—and also to barley plants useful in the preparation of such beverages, as well as other plant products prepared from said plants. Utilization of the invention clears the way for making improved production procedures of beverages with improved taste profiles, and promises also for notable reductions in the thermal energy input for production of beer.

3. 发明授权

US07420105B2 Barley for production of flavor-stable beverage 有权
标题翻译：大麦生产风味稳定的饮料
公开(公告)号：US07420105B2
公开(公告)日：2008-09-02
申请号：US10800200
申请日：2004-03-11
申请人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Soren Knudsen , Lene Molskov Bech
发明人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Soren Knudsen , Lene Molskov Bech
IPC分类号： A01H5/00 , C12N15/82 , C12C1/00 , C12C11/00
CPC分类号： C12C1/18 , A01H5/10 , C12C5/004 , C12C7/04 , C12C7/047 , C12C11/003 , C12C12/00 , C12N9/0069 , C12N15/8242 , C12N15/8243
摘要： According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off-flavors, even after prolonged storage of the beverage.
摘要翻译：根据本发明，提供了无效LOX-1大麦及其生产的植物产品，例如通过使用缺乏合成脂肪酸转化酶脂氧合酶-1的大麦籽粒制备的麦芽。所述酶涉及与将亚油酸转化为9-氢过氧十八碳二烯酸（脂氧合酶途径代谢物）相关的主要活性，其通过进一步的酶促或自发反应可能导致反式-2-壬烯醛的出现。本发明使酿造商能够生产没有可检测的反式-2-壬烯醛特异性异味的啤酒，即使在饮料长期储存之后。

4. 发明申请

US20110318469A1 Barley with reduced lipoxygenase activity and beverage prepared therefrom 有权
标题翻译：具有降低的脂氧合酶活性的大麦和由其制备的饮料
公开(公告)号：US20110318469A1
公开(公告)日：2011-12-29
申请号：US13141579
申请日：2009-12-28
申请人： Birgitte Skadhauge , Finn Lok , Klaus Breddam , Ole Olsen , Lene Molskov Bech , Soren Knudsen
发明人： Birgitte Skadhauge , Finn Lok , Klaus Breddam , Ole Olsen , Lene Molskov Bech , Soren Knudsen
IPC分类号： A01H5/00 , C12C12/00 , C12C1/027 , C12C7/00 , A01H1/04 , C12C1/18
CPC分类号： A01H5/10 , C12C1/18 , C12C12/00 , C12C2200/01
摘要： According to the invention, there is provided barley with total loss of functional lipoxygenase (LOX)-1 and LOX-2 enzymes, and plant products produced thereof, such as malt manufactured by using barley kernels defective in the synthesis of the fatty acid-dioxygenating enzymes LOX-1 and LOX-2. Said enzymes account for the principal activities related to dioxygenation of linoleic acid into 9- and 13-hydroperoxy octadecadienoic acid, respectively. 9-Hydroperoxy octadecadienoic acid represents a LOX pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal (T2N). The invention enables brewers to produce a beer having insignificant levels of stale, T2N-specific off-flavors, even after prolonged storage of the beverage.
摘要翻译：根据本发明，提供了具有功能性脂氧合酶（LOX）-1和LOX-2酶的总损失的大麦及其生产的植物产品，例如通过使用在合成脂肪酸 - 双氧合中缺陷的大麦仁制备的麦芽酶LOX-1和LOX-2。所述酶分别涉及与亚油酸双加氧相关的9-和13-氢过氧十八碳二烯酸的主要活性。 9-氢过氧十八碳二烯酸代表LOX途径代谢物，通过进一步的酶促或自发反应，可能导致反式-2-壬醛（T2N）的出现。本发明使酿造商能够生产具有不明显水平的陈年T2N特异性异味的啤酒，即使在饮料长期储存之后。

5. 发明申请

US20090029000A1 Barley for Production of Flavor-Stable Beverage 有权
标题翻译：大麦生产调味饮料
公开(公告)号：US20090029000A1
公开(公告)日：2009-01-29
申请号：US10598779
申请日：2005-03-09
申请人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Soren Knudsen , Lene Molskov Bech
发明人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Soren Knudsen , Lene Molskov Bech
IPC分类号： A01H5/00 , C12N15/82 , C12C11/00 , C12C1/00 , A23L2/00 , A23K1/00 , A23L1/48
CPC分类号： C12C1/18 , A01H5/10 , C12C5/004 , C12C7/04 , C12C7/047 , C12C11/003 , C12C12/00 , C12N9/0069 , C12N15/8242 , C12N15/8243
摘要： According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.
摘要翻译：根据本发明，提供了无效LOX-1大麦及其生产的植物产品，例如通过使用缺乏合成脂肪酸转化酶脂氧合酶-1的大麦籽粒制备的麦芽。所述酶涉及与亚油酸转化为9-氢过氧十八碳二烯酸（脂氧合酶途径代谢物）相关的主要活性，其通过进一步的酶促或自发反应可导致反式-2-壬烯醛的出现。本发明使酿造商能够生产没有可检测的反式-2-壬烯醛特异性异味的啤酒，甚至在长时间储存饮料之后。

6. 发明申请

US20110195151A1 BARLEY FOR PRODUCTION OF FLAVOR-STABLE BEVERAGE 审中-公开
标题翻译： BARLEY生产易挥发性饮料
公开(公告)号：US20110195151A1
公开(公告)日：2011-08-11
申请号：US12951569
申请日：2010-11-22
申请人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Søren Knudsen , Lene Mølskov Bech
发明人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Søren Knudsen , Lene Mølskov Bech
IPC分类号： A01H5/00 , C12C1/00 , C12C7/04 , C11B9/02
CPC分类号： C12C1/18 , A01H5/10 , C12C5/004 , C12C7/04 , C12C7/047 , C12C11/003 , C12C12/00 , C12N9/0069 , C12N15/8242 , C12N15/8243
摘要： According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.
摘要翻译：根据本发明，提供了无效LOX-1大麦及其生产的植物产品，例如通过使用缺乏合成脂肪酸转化酶脂氧合酶-1的大麦籽粒制备的麦芽。所述酶涉及与将亚油酸转化为9-氢过氧十八碳二烯酸（脂氧合酶途径代谢物）相关的主要活性，其通过进一步的酶促或自发反应可能导致反式-2-壬烯醛的出现。本发明使酿造商能够生产没有可检测的反式-2-壬烯醛特异性异味的啤酒，甚至在长时间储存饮料之后。

7. 发明授权

US09363959B2 Barley with reduced lipoxygenase activity and beverage prepared therefrom 有权
标题翻译：具有降低的脂氧合酶活性的大麦和由其制备的饮料
公开(公告)号：US09363959B2
公开(公告)日：2016-06-14
申请号：US13141579
申请日：2009-12-28
申请人： Birgitte Skadhauge , Finn Lok , Klaus Breddam , Ole Olsen , Lette Mølskov Bech , Søren Knudsen
发明人： Birgitte Skadhauge , Finn Lok , Klaus Breddam , Ole Olsen , Lette Mølskov Bech , Søren Knudsen
IPC分类号： A01H5/10 , C12C1/18 , C12C12/00
CPC分类号： A01H5/10 , C12C1/18 , C12C12/00 , C12C2200/01
摘要： According to the invention, there is provided barley with total loss of functional lipoxygenase (LOX)-1 and LOX-2 enzymes, and plant products produced thereof, such as malt manufactured by using barley kernels defective in the synthesis of the fatty acid-dioxygenating enzymes LOX-1 and LOX-2. Said enzymes account for the principal activities related to dioxygenation of linoleic acid into 9- and 13-hydroperoxy octadecadienoic acid, respectively. 9-Hydroperoxy octadecadienoic acid represents a LOX pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal (T2N). The invention enables brewers to produce a beer having insignificant levels of stale, T2N-specific off-flavors, even after prolonged storage of the beverage.
摘要翻译：根据本发明，提供了具有功能性脂氧合酶（LOX）-1和LOX-2酶的总损失的大麦及其生产的植物产品，例如通过使用在合成脂肪酸 - 双氧合中缺陷的大麦仁制备的麦芽酶LOX-1和LOX-2。所述酶分别涉及与亚油酸双加氧相关的9-和13-氢过氧十八碳二烯酸的主要活性。 9-氢过氧十八碳二烯酸代表LOX途径代谢物，通过进一步的酶促或自发反应，可能导致反式-2-壬醛（T2N）的出现。本发明使酿造商能够生产具有不明显水平的陈年T2N特异性异味的啤酒，即使在饮料长期储存之后。

8. 发明授权

US07838053B2 Barley for production of flavor-stable beverage 有权
标题翻译：大麦生产风味稳定的饮料
公开(公告)号：US07838053B2
公开(公告)日：2010-11-23
申请号：US10598779
申请日：2005-03-09
申请人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Søren Knudsen , Lene Mølskov Bech
发明人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Søren Knudsen , Lene Mølskov Bech
IPC分类号： C12C11/00 , C12C1/00 , A23L1/00 , A23L1/48
CPC分类号： C12C1/18 , A01H5/10 , C12C5/004 , C12C7/04 , C12C7/047 , C12C11/003 , C12C12/00 , C12N9/0069 , C12N15/8242 , C12N15/8243
摘要： According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.
摘要翻译：根据本发明，提供了无效LOX-1大麦及其生产的植物产品，例如通过使用缺乏合成脂肪酸转化酶脂氧合酶-1的大麦籽粒制备的麦芽。所述酶涉及与将亚油酸转化为9-氢过氧十八碳二烯酸（脂氧合酶途径代谢物）相关的主要活性，其通过进一步的酶促或自发反应可能导致反式-2-壬烯醛的出现。本发明使酿造商能够生产没有可检测的反式-2-壬烯醛特异性异味的啤酒，甚至在长时间储存饮料之后。

9. 发明申请

US20050204437A1 Barley for production of flavor-stable beverage 有权
标题翻译：大麦生产风味稳定的饮料
公开(公告)号：US20050204437A1
公开(公告)日：2005-09-15
申请号：US10800200
申请日：2004-03-11
申请人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Soren Knudsen , Lene Bech
发明人： Klaus Breddam , Ole Olsen , Birgitte Skadhauge , Finn Lok , Soren Knudsen , Lene Bech
IPC分类号： A01H5/10 , C12C1/18 , C12N9/02 , C12N15/82 , A01H5/00 , A23K1/00
CPC分类号： C12C1/18 , A01H5/10 , C12C5/004 , C12C7/04 , C12C7/047 , C12C11/003 , C12C12/00 , C12N9/0069 , C12N15/8242 , C12N15/8243
摘要： According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off-flavors, even after prolonged storage of the beverage.
摘要翻译：根据本发明，提供了无效LOX-1大麦及其生产的植物产品，例如通过使用缺乏合成脂肪酸转化酶脂氧合酶-1的大麦籽粒制备的麦芽。所述酶涉及与将亚油酸转化为9-氢过氧十八碳二烯酸（脂氧合酶途径代谢物）相关的主要活性，其通过进一步的酶促或自发反应可能导致反式-2-壬烯醛的出现。本发明使酿造商能够生产没有可检测的反式-2-壬烯醛特异性异味的啤酒，即使在饮料长期储存之后。

10. 发明授权

US06187579B1 Customized proteases 失效
标题翻译：定制蛋白酶
公开(公告)号：US06187579B1
公开(公告)日：2001-02-13
申请号：US08329892
申请日：1994-10-27
申请人： Klaus Breddam , Morten C. Kielland-Brandt , Uffe Hasbo Mortensen , Kjeld Ove Olesen , Henning Ralf Stennicke , Fred W. Wagner
发明人： Klaus Breddam , Morten C. Kielland-Brandt , Uffe Hasbo Mortensen , Kjeld Ove Olesen , Henning Ralf Stennicke , Fred W. Wagner
IPC分类号： C12N960
CPC分类号： C12N15/102 , C07K14/60 , C12N9/48 , C12N15/10 , C12P21/02
摘要： The invention provides customized proteases (i.e., mutant enzymes), methods of making customized proteases, as well as methods of using customized proteases. The customized proteases of the invention are derived from the known proteases. Altered transacylation reactions include the capability to perform transacylation reactions not substantially catalyzed by the known protease or the capability to perform transacylation reactions with improved yields, or both. The methods of the invention provide for customized proteases through site specific or random mutagenesis of the active site amino acids of the known proteases. The invention also provides for methods of using the customized proteases to prepare a preselected transacylation products. The preselected transacylation products produced can be modified by substitution at the N-or C-terminal with nucleophiles such as L-amino acids, D-amino acids, amino acid amides, and radioactive amino acids.
摘要翻译：本发明提供定制的蛋白酶（即突变酶），制备定制蛋白酶的方法以及使用定制的蛋白酶的方法。本发明的定制的蛋白酶来自已知的蛋白酶。改变的转酰基反应包括执行基本上不被已知蛋白酶催化的转酰基化反应的能力或者以提高的产量进行转酰化反应的能力，或两者都有。本发明的方法通过已知蛋白酶的活性位点氨基酸的位点特异性或随机诱变提供定制的蛋白酶。本发明还提供使用定制的蛋白酶制备预选的转酰胺产物的方法。所产生的预选择的转酰胺化产物可以通过用亲核试剂如L-氨基酸，D-氨基酸，氨基酸酰胺和放射性氨基酸在N-或C-末端取代来修饰。

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