专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06268166B1 Method of quantitative assay for 1,5-anhydroglucitol 失效
标题翻译： 1,5-脱水葡萄糖醇的定量测定方法
公开(公告)号：US06268166B1
公开(公告)日：2001-07-31
申请号：US08230440
申请日：1994-04-19
申请人： Ryo Kojima , Yoshiro Sato , Takeshi Nagasawa
发明人： Ryo Kojima , Yoshiro Sato , Takeshi Nagasawa
IPC分类号： C12Q126
CPC分类号： C12Q1/26 , C12P7/02
摘要： There is provided an enzymatic method of rapid quantitative assay for 1,5-anhydroglucitol which is also applicable to an automated analysis device. The enzymatic method of quantitative assay for 1,5-anhydroglucitol is characterized by using a pyranose oxidase derived from Basidiomycetous fungi No. 52 as a pyranose oxidase. According to the assaying method of the present invention, assay for 1,5-anhydroglucitol in a specimen can be performed using an automated analysis device, results in rapid assay and improved accuracy.
摘要翻译：提供了用于1,5-脱水葡萄糖醇的快速定量测定法的酶法，其也适用于自动化分析装置。 1,5-脱水葡萄糖醇的定量测定法的特征在于使用源自Basidiomycetous真菌No.52的吡喃糖氧化酶作为吡喃糖氧化酶。根据本发明的测定方法，可以使用自动分析装置进行样品中1,5-脱水葡萄糖醇的测定，结果快速测定并提高精度。

2. 发明授权

US5486458A Method of quantitative assay for 1,5-anhydroglucitol 失效
标题翻译： 1,5-脱水葡萄糖醇的定量测定方法
公开(公告)号：US5486458A
公开(公告)日：1996-01-23
申请号：US85317
申请日：1993-06-30
申请人： Ryo Kojima , Yoshiro Sato , Takeshi Nagasawa
发明人： Ryo Kojima , Yoshiro Sato , Takeshi Nagasawa
IPC分类号： C12Q1/26 , C12Q1/34 , C12Q1/54 , C12N1/00
CPC分类号： C12Q1/26 , C12Q1/34 , C12Q1/54 , G01N2333/924 , Y10S435/81 , Y10S435/832 , Y10S435/911 , Y10T436/25 , Y10T436/25125
摘要： There is provided a method of quantitative assay for 1,5-anhydroglucitol in a specimen characterized by effectively removing maltose present in the specimen using .alpha.-glucosidase. Maltose present in a specimen is previously converted into glucose by the action of .alpha.-glucosidase derived from a microorganism belonging to the genus Bacillus to remove the affect by maltose. Then 1,5-anhydroglucitol in the specimen is quantitatively determined.
摘要翻译：提供了一种在样品中定量测定1,5-脱水葡萄糖醇的方法，其特征在于使用α-葡糖苷酶有效除去样品中存在的麦芽糖。存在于标本中的麦芽糖通过衍生自属于芽孢杆菌属的微生物的α-葡糖苷酶的作用而转化为葡萄糖，以消除麦芽糖的影响。然后定量测定样品中的1,5-脱水葡萄糖醇。

3. 发明授权

US5468621A Method of quantitative assay for 1,5-anhydroglucitol 失效
标题翻译： 1,5-脱水葡萄糖醇的定量测定方法
公开(公告)号：US5468621A
公开(公告)日：1995-11-21
申请号：US21250
申请日：1993-02-23
申请人： Ryo Kojima , Yoshiro Sato , Takeshi Nagasawa
发明人： Ryo Kojima , Yoshiro Sato , Takeshi Nagasawa
IPC分类号： C12Q1/26 , C12Q1/48 , C12R1/645 , C12Q1/28
CPC分类号： C12Q1/26 , Y10S435/962
摘要： A quantitative assay for 1,5-anhydroglucitol in a specimen is described. This assay comprises treating a specimen with a phosphorylating enzyme in the presence of 5 to 500 mM adenosine-5'-triphosphate in a pH range of 7.2 to 8.5, to selectively eliminate sugars other than 1,5-anhydroglucitol, and reacting the product of this reaction, without removing the residual ATP, with pyranose oxidase in the above pH range. This assay is quite rapid and suitable for performance using an automated device.
摘要翻译：描述了样品中1,5-脱水葡萄糖醇的定量测定。该测定包括在pH范围为7.2至8.5的5至500mM腺苷-5'-三磷酸的存在下用磷酸化酶处理样品，以选择性除去1,5-脱水葡萄糖醇以外的糖，并使该反应在上述pH范围内不用吡喃糖氧化酶除去残留的ATP。该测定非常快速，适用于使用自动化设备的性能。

4. 发明授权

US5869276A Method for determination of direct bilirubin and reagent therefor 失效
标题翻译：测定直接胆红素及其试剂的方法
公开(公告)号：US5869276A
公开(公告)日：1999-02-09
申请号：US90332
申请日：1998-06-04
申请人： Ryo Kojima , Yoshikiyo Sasagawa , Yasunori Okazaki , Takeshi Nagasawa
发明人： Ryo Kojima , Yoshikiyo Sasagawa , Yasunori Okazaki , Takeshi Nagasawa
IPC分类号： C12Q1/26 , C12Q1/00 , G01N33/53
CPC分类号： C12Q1/26 , Y10S435/961
摘要： There is provided a method for determination of direct bilirubin which comprises the steps of contacting a bilirubin oxidase with a sample suspected of containing bilirubin; and, measuring direct bilirubin in the sample by optical changes of the sample, characterized in that bilirubin oxidase is allowed to act in the presence of a indirect bilirubin reaction inhibitor selected from a thiocyanate ion, a hydrazide, reduced nicotinamide adenine dinucleotide, reduced nicotinamide adenine dinucleotide phosphate and a potassium ion of 100 mM to 800 mM. By completely avoiding interference of indirect bilirubin, direct bilirubin is selectively and precisely determined using a reagent kit in the form of solution. The method and reagent of the invention are safe and free of environmental pollution due to unnecessary waste liquid treatment.
摘要翻译：提供了用于测定直接胆红素的方法，其包括使胆红素氧化酶与怀疑含有胆红素的样品接触的步骤; 并且通过样品的光学变化测量样品中的直接胆红素，其特征在于允许胆红素氧化酶在选自硫氰酸根离子，酰肼，还原型烟酰胺腺嘌呤二核苷酸，还原型烟酰胺腺嘌呤的间接胆红素反应抑制剂存在下起作用二磷酸核苷酸和100mM至800mM的钾离子。通过完全避免间接胆红素的干扰，使用溶液形式的试剂盒选择性和精确地测定直接胆红素。本发明的方法和试剂是安全的，由于不必要的废液处理而没有环境污染。

5. 发明申请

US20110287335A1 REINFORCED ELECTROLYTE MEMBRANE FOR FUEL CELL, MEMBRANE-ELECTRODE ASSEMBLY FOR FUEL CELL, AND POLYMER ELECTROLYTE FUEL CELL COMPRISING THE SAME 有权
标题翻译：用于燃料电池的加强电解质膜，用于燃料电池的膜电极组件和包含其的聚合物电解质燃料电池
公开(公告)号：US20110287335A1
公开(公告)日：2011-11-24
申请号：US13124602
申请日：2009-10-15
申请人： Yasuhiro Akita , Masanori Aimu , Tatsuya Kawahara , Takeshi Nagasawa , Toyohiro Matsuura , Takeyuki Suzuki , Hiroshi Kato , Hideki Yamada
发明人： Yasuhiro Akita , Masanori Aimu , Tatsuya Kawahara , Takeshi Nagasawa , Toyohiro Matsuura , Takeyuki Suzuki , Hiroshi Kato , Hideki Yamada
IPC分类号： H01M8/10
CPC分类号： H01M8/10 , C08J5/2206 , C08J2327/18 , H01B1/122 , H01M8/1051 , H01M8/106 , H01M8/1062 , H01M8/1081 , Y02P70/56
摘要： It is an object of the present invention to provide a fuel cell electrolyte membrane reinforced with a porous substrate which has excellent durability and in which the amount of cross leakage as a result of chemical deterioration of electrolyte membrane components due to the presence of peroxide and/or radicals is particularly reduced. The present invention relates to an electrolyte membrane for a fuel cell comprising a polyelectrolyte, which contains a porous substrate and a radical scavenger dispersed in the polyelectrolyte.
摘要翻译：本发明的目的是提供一种用多孔基材增强的燃料电池电解质膜，其具有优异的耐久性，并且由于过氧化物和/或氧化物的存在而导致电解质膜组分的化学劣化导致的交叉泄漏量，或自由基特别减少。本发明涉及一种包含聚电解质的燃料电池用电解质膜，该聚电解质含有分散在聚电解质中的多孔基材和自由基清除剂。

6. 发明申请

US20110008708A1 REINFORCED ELECTROLYTE MEMBRANE FOR FUEL CELL, FUEL CELL MEMBRANE-ELECTRODE ASSEMBLY, AND SOLID POLYMER ELECTROLYTE FUEL CELL COMPRISING THE FUEL CELL MEMBRANE-ELECTRODE ASSEMBLY 有权
标题翻译：用于燃料电池，燃料电池电极组件和包含燃料电池组件的固体聚合物电解质燃料电池的增强电解质膜
公开(公告)号：US20110008708A1
公开(公告)日：2011-01-13
申请号：US12919276
申请日：2008-10-17
申请人： Yasuhiro Akita , Takeshi Nagasawa , Takeyuki Suzuki , Toyohiro Matsuura
发明人： Yasuhiro Akita , Takeshi Nagasawa , Takeyuki Suzuki , Toyohiro Matsuura
IPC分类号： H01M8/10
CPC分类号： H01B1/122 , C08J5/2206 , C08J2327/18 , H01M8/1023 , H01M8/1039 , H01M8/1048 , H01M8/106 , H01M8/1062 , H01M8/1067 , H01M8/1079 , Y02P70/56
摘要： The present invention provides a reinforced electrolyte membrane for fuel cell comprising a porous substrate impregnated with a polyelectrolyte liquid dispersion, wherein either the maximum tensile strength in the machine direction (for sheet processing) (MD) or the maximum tensile strength in the transverse direction (TD; vertical to the MD direction) for the electrolyte membrane is 70 N/mm2 or more at 23° C. at a relative humidity of 50% or 40 N/mm2 or more at 80° C. at a relative humidity of 90%. This reinforced electrolyte membrane for fuel cell, in which the amount of fluorine ions eluted as a result of deterioration of electrolyte membrane components in particular is reduced, has excellent durability.
摘要翻译：本发明提供一种用于燃料电池的增强电解质膜，其包括浸渍有聚电解质液体分散体的多孔基材，其中在纵向（用于片材加工）（MD）或在横向上的最大拉伸强度在相对湿度90％的相对湿度为50％或40N / mm 2以上的情况下，电解质膜的电阻率为70N / mm 2以上，相对湿度90％。这种用于燃料电池的增强电解质膜特别是由于电解质膜组分的劣化而洗脱的氟离子的量减少，具有优异的耐久性。

7. 发明申请

US20070231446A1 Method for producing oil and fat composition with reduced trans-fatty acid content and processed oil and fat product containing the oil and fat composition 审中-公开
标题翻译：生产具有降低的反式脂肪酸含量的油脂组合物的方法，含有油脂组合物的加工油脂产品
公开(公告)号：US20070231446A1
公开(公告)日：2007-10-04
申请号：US11806222
申请日：2007-05-30
申请人： Takeshi Nagasawa , Akira Akahane , Kaori Muroga , Masahiro Arikai
发明人： Takeshi Nagasawa , Akira Akahane , Kaori Muroga , Masahiro Arikai
IPC分类号： A23D9/00
CPC分类号： C12P7/6454 , A23D9/00 , C11C3/10
摘要： A method for producing an oil and fat composition includes the steps of (a) mixing an oil and fat (A) wherein fatty acids with 14 or less carbon atoms are contained in an amount of 50 to 100% by mass of the entire constituent fatty acids of the triglycerides with an oil and fat (B) wherein fatty acids with 16 to 24 carbon atoms are contained in an amount of 80 to 100% by mass of the entire constituent fatty acids of the triglycerides to obtain an oil and fat mixture; (b) subjecting the oil and fat mixture to random transesterification using an enzyme having no positional specificity, and (c) obtaining an oil and fat composition free from trans-fatty acid.
摘要翻译：油脂组合物的制造方法包括以下步骤：（a）将含有14个以下碳原子的脂肪酸的油脂（A）以50〜100质量％甘油三酸酯与油脂（B）的酸，其中含有16至24个碳原子的脂肪酸的量为甘油三酯的全部构成脂肪酸的80至100质量％，以获得油脂混合物; （b）使用不具有位置特异性的酶对油脂混合物进行无规酯交换反应，和（c）获得不含反式脂肪酸的油脂组合物。

8. 发明授权

US5719030A Monoclonal antibody to prostate-derived acid phosphatase and method for determination of acid phosphatase using the same as well as kit for determination 失效
标题翻译：用于前列腺来源的酸性磷酸酶的单克隆抗体以及用于测定酸性磷酸酶的方法以及用于测定的试剂盒
公开(公告)号：US5719030A
公开(公告)日：1998-02-17
申请号：US380243
申请日：1995-01-26
申请人： Katsumasa Kuroiwa , Katsuhiro Katayama , Takahiro Tomiyama , Toshihide Miura , Takeshi Nagasawa
发明人： Katsumasa Kuroiwa , Katsuhiro Katayama , Takahiro Tomiyama , Toshihide Miura , Takeshi Nagasawa
IPC分类号： C12N5/10 , C07K16/40 , C12N5/00 , C12N15/02 , C12P21/08 , C12Q1/42 , C12R1/91 , G01N33/53 , G01N33/573 , G01N33/577 , C07K16/00
CPC分类号： C07K16/40 , C12Q1/42 , G01N33/573
摘要： According to the present invention, there are provided a monoclonal antibody to prostate-derived acid phosphatase and a method for determination of prostate-derived acid phosphatase using the monoclonal antibody. Prostate-derived acid phosphatase is boostered in animal and spleen cells isolated from the animal are fused with myeloma cells. The hybridoma capable of producing a monoclonal antibody having extremely high specificity to prostate-derived acid phosphatase is subjected to cloning. Using the monoclonal antibody produced by the hybridoma, prostate-derived acid phosphatase in a sample can be detected with extremely high sensitivity.
摘要翻译：根据本发明，提供了前列腺来源的酸性磷酸酶的单克隆抗体和使用单克隆抗体测定前列腺衍生的酸性磷酸酶的方法。前列腺来源的酸性磷酸酶在动物中增强，从动物分离的脾细胞与骨髓瘤细胞融合。能够产生对前列腺来源的酸性磷酸酶具有极高特异性的单克隆抗体的杂交瘤进行克隆。使用由杂交瘤产生的单克隆抗体，可以以极高的灵敏度检测样品中的前列腺来源的酸性磷酸酶。

9. 发明授权

US5416238A L-argininal acetal derivatives and a process for production thereof 失效
标题翻译： L-精氨酸缩醛衍生物及其制备方法
公开(公告)号：US5416238A
公开(公告)日：1995-05-16
申请号：US755527
申请日：1991-09-05
申请人： Yoshihito Abe , Akio Hashimoto , Takeshi Nagasawa , Katsumasa Kuroiwa
发明人： Yoshihito Abe , Akio Hashimoto , Takeshi Nagasawa , Katsumasa Kuroiwa
IPC分类号： C07C279/12 , B01J31/02 , C07B61/00 , C07C277/08 , C07C279/24 , C07C209/78
CPC分类号： C07C279/12 , C07C279/24 , Y02P20/55
摘要： Novel N.sup.G -protected or unprotected-L-argininal-dibutyl acetals (I) can be prepared from L-argininal derivatives (III) merely by reacting in an azeotropic solvent in the presence of p-toluenesulfonic acid. In the products (I), L-form amounts to more than 99%, without further optical resolution. The L-argininal acetals (I) are useful as starting materials for preparing peptidyl-L-argininal derivatives which can provide protease inhibitors or ligands used for affinity chromatography of various proteases.
摘要翻译：只有通过在对甲苯磺酸存在下在共沸溶剂中反应，可以由L-精氨酸衍生物（III）制备新的NG-保护的或未受保护的L-精氨酸 - 二丁基缩醛（I）。在产品（I）中，L型大于99％，无需进一步的光学拆分。 L-精氨酸缩醛（I）可用作制备肽基-L-精氨酸衍生物的起始原料，其可以提供蛋白酶抑制剂或用于各种蛋白酶亲和层析的配体。

10. 发明授权

US5399488A Method for assaying enzyme activity using N-acetyl-.beta.-D-glucosamine derivatives 失效
标题翻译：使用N-乙酰基-β-D-葡糖胺衍生物测定酶活性的方法
公开(公告)号：US5399488A
公开(公告)日：1995-03-21
申请号：US248856
申请日：1994-05-23
申请人： Katsumasa Kuroiwa , Katsuhiro Katayama , Toshihide Miura , Takeshi Nagasawa
发明人： Katsumasa Kuroiwa , Katsuhiro Katayama , Toshihide Miura , Takeshi Nagasawa
IPC分类号： C07H17/02 , C12Q1/34 , C07H5/10 , C07H19/048
CPC分类号： C07H17/02 , C12Q1/34 , C12Q2334/00 , G01N2333/924
摘要： Novel N-acetyl-.beta.-D-glucosamine derivatives and a method for assaying N-acetyl-.beta.-D-glucosaminidase activity using the same as substrate are provided.N-Acetyl-.beta.-D-glucosamine derivatives represented by general formula (I): ##STR1## wherein each of R.sub.1 and R.sub.2 independently represents hydrogen atom or methyl or ethyl group, are mixed with a sample containing N-acetyl-.beta.-D-glucosaminidase (NAG) and then absorbance of the reaction solution is determined to assay NAG activity extremely accurately.
摘要翻译：提供新的N-乙酰基-β-D-葡糖胺衍生物和使用与底物相同的N-乙酰基-β-D-葡糖胺酶活性的方法。由通式（I）表示的N-乙酰基-β-D-葡糖胺衍生物：其中R1和R2各自独立地表示氢原子或甲基或乙基，与含有N-乙酰基 - β-D-氨基葡糖苷酶（NAG），然后测定反应溶液的吸光度以极精确地测定NAG活性。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式