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1. 发明授权

US08506793B2 Cardiomyocytes-containing device and method for manufacturing and using the same 失效
标题翻译：含有心肌细胞的装置及其制造和使用方法
公开(公告)号：US08506793B2
公开(公告)日：2013-08-13
申请号：US13147607
申请日：2010-02-02
申请人： Ronald Dekker , Anja Van De Stolpe
发明人： Ronald Dekker , Anja Van De Stolpe
IPC分类号： G01N27/327
CPC分类号： G01N1/30 , C12N5/0657 , C12N2503/02 , C12N2535/10 , G01N33/5061
摘要： Disclosed is a device for determining the cardiotoxicity of a chemical compound, comprising a substrate (10) carrying a deformable stack (34), said stack being partially detached from the substrate by a cavity (32) allowing an out-of-plane deformation of the stack, said stack comprising a first deformable layer (16), a second deformable layer (20) and a multi-electrode structure (18) sandwiched between the first and second deformable layers, the second deformable layer carrying a pattern of cardiomyocytes (28) adhered thereto; and a liquid container (26) mounted on the substrate for exposing the cardiomyocytes to the chemical compound. A method of manufacturing such a device is also disclosed. The present invention further relates to the use of the device for drug target discovery and/or drug development and a method for developing a disease model for a disease that is caused by or modified by stretching of cells, in particular a cardiac disease model.
摘要翻译：公开了一种用于确定化合物的心脏毒性的装置，包括承载可变形叠层（34）的基底（10），所述叠层通过空腔（32）部分地从基底分离，所述堆叠包括夹在所述第一和第二可变形层之间的第一可变形层（16），第二可变形层（20）和多电极结构（18），所述第二可变形层承载心肌细胞图案（28 ）和安装在基板上的用于将心肌细胞暴露于化合物的液体容器（26）。还公开了制造这种装置的方法。本发明还涉及该装置用于药物靶发现和/或药物开发的用途，以及用于开发由细胞拉伸，特别是心脏疾病模型引起或改变的疾病的疾病模型的方法。

2. 发明申请

US20120094323A1 CARDIOMYOCYTES-CONTAINING DEVICE AND METHOD FOR MANUFACTURING AND USING THE SAME 失效
标题翻译：含有血小板活性的装置及其制造和使用方法
公开(公告)号：US20120094323A1
公开(公告)日：2012-04-19
申请号：US13147607
申请日：2010-02-02
申请人： Ronald Dekker , Anja Van De Stolpe
发明人： Ronald Dekker , Anja Van De Stolpe
IPC分类号： C12Q1/18 , H01B13/00 , B05D5/00 , C12M1/34 , B05D1/36
CPC分类号： G01N1/30 , C12N5/0657 , C12N2503/02 , C12N2535/10 , G01N33/5061
摘要： Disclosed is a device for determining the cardiotoxicity of a chemical compound, comprising a substrate (10) carrying a deformable stack (34), said stack being partially detached from the substrate by a cavity (32) allowing an out-of-plane deformation of the stack, said stack comprising a first deformable layer (16), a second deformable layer (20) and a multi-electrode structure (18) sandwiched between the first and second deformable layers, the second deformable layer carrying a pattern of cardiomyocytes (28) adhered thereto; and a liquid container (26) mounted on the substrate for exposing the cardiomyocytes to the chemical compound. A method of manufacturing such a device is also disclosed. The present invention further relates to the use of the device for drug target discovery and/or drug development and a method for developing a disease model for a disease that is caused by or modified by stretching of cells, in particular a cardiac disease model.
摘要翻译：公开了一种用于确定化合物的心脏毒性的装置，包括承载可变形叠层（34）的基底（10），所述叠层通过空腔（32）部分地与基底分离，允许外部变形所述堆叠包括夹在所述第一和第二可变形层之间的第一可变形层（16），第二可变形层（20）和多电极结构（18），所述第二可变形层承载心肌细胞图案（28 ）和安装在基板上的用于将心肌细胞暴露于化合物的液体容器（26）。还公开了制造这种装置的方法。本发明还涉及该装置用于药物靶发现和/或药物开发的用途，以及用于开发由细胞拉伸，特别是心脏疾病模型引起或改变的疾病的疾病模型的方法。

3. 发明授权

US10209243B2 Cardiomyocyte containing device, manufacturing method and measuring method 有权
公开(公告)号：US10209243B2
公开(公告)日：2019-02-19
申请号：US13813970
申请日：2011-07-13
申请人： Ronald Dekker , Anja Van De Stolpe , Berent Jan Van Meer , Saeed Khoshefetrat Pakazad , Angel Savov
发明人： Ronald Dekker , Anja Van De Stolpe , Berent Jan Van Meer , Saeed Khoshefetrat Pakazad , Angel Savov
IPC分类号： B01L1/00 , B01L3/00 , G01N33/50 , D06F75/22
摘要： Disclosed is a device (100) for cardiac electrophysiology screening comprising a substrate (10) comprising a cavity (42), said substrate carrying a deformable layer (32) extending over said cavity (42), wherein a portion of said deformable layer (32) covers said cavity and acts as a membrane over said cavity (32), said portion having a surface comprising a pattern of grooves (44) and carrying a multi-electrode structure (110, 110′); and a plurality of cardiomyocytes (130) assembled in at least some of said grooves (44). A method of manufacturing such a device (100) is also disclosed.

4. 发明申请

US20130137132A1 CARDIOMYOCYTE CONTAINING DEVICE, MANUFACTURING METHOD AND MEASURING METHOD 审中-公开
标题翻译：含有骨髓细胞的装置，制造方法和测量方法
公开(公告)号：US20130137132A1
公开(公告)日：2013-05-30
申请号：US13813970
申请日：2011-07-13
申请人： Ronald Dekker , Anja Van De Stolpe , Berent Jan Van Meer , Saeed Khoshefetrat Pakazad , Angel Savov
发明人： Ronald Dekker , Anja Van De Stolpe , Berent Jan Van Meer , Saeed Khoshefetrat Pakazad , Angel Savov
IPC分类号： C12Q1/02
CPC分类号： G01N33/5061 , D06F75/22
摘要： Disclosed is a device (100) for cardiac electrophysiology screening comprising a substrate (10) comprising a cavity (42), said substrate carrying a deformable layer (32) extending over said cavity (42), wherein a portion of said deformable layer (32) covers said cavity and acts as a membrane over said cavity (32), said portion having a surface comprising a pattern of grooves (44) and carrying a multi-electrode structure (110, 110′); and a plurality of cardiomyocytes (130) assembled in at least some of said grooves (44). A method of manufacturing such a device (100) is also disclosed.
摘要翻译：公开了一种用于心脏电生理筛选的装置（100），包括：衬底（10），包括空腔（42），所述衬底承载在所述空腔（42）上延伸的可变形层（32），其中所述可变形层）覆盖所述空腔并用作所述空腔（32）上的膜，所述部分具有包括凹槽图案（44）并且承载多电极结构（110,110'）的表面; 以及组装在至少一些所述凹槽（44）中的多个心肌细胞（130）。还公开了制造这种装置（100）的方法。

5. 发明授权

US11443831B2 Assessment of cellular signaling pathway activity using probabilistic modeling of target gene expression 有权
公开(公告)号：US11443831B2
公开(公告)日：2022-09-13
申请号：US14233546
申请日：2012-07-19
申请人： Wilhelmus Franciscus Johannes Verhaegh , Anja Van De Stolpe , Hendrik Jan Van Ooijen , Kalyana Chakravarthi Dulla , Marcia Alves De Inda , Ralf Hoffmann
发明人： Wilhelmus Franciscus Johannes Verhaegh , Anja Van De Stolpe , Hendrik Jan Van Ooijen , Kalyana Chakravarthi Dulla , Marcia Alves De Inda , Ralf Hoffmann
IPC分类号： G16B5/20 , G16B5/00 , G16B20/00 , C12Q1/6809 , C12Q1/6886 , G16B40/00 , G16B20/30 , G16B40/20 , G16B20/20 , G16B25/00
摘要： The present application mainly relates to specific methods for inferring activity of one or more cellular signaling pathway(s) in tissue of a medical subject based at least on the expression level(s) of one or more target gene(s) of the cellular signaling pathway(s) measured in an extracted sample of the tissue of the medical subject, an apparatus comprising a digital compressor configured to perform such methods and a non-transitory storage medium storing instructions that are executable by a digital processing device to perform such methods.

6. 发明授权

US08932811B2 Genomic selection and sequencing using encoded microcarriers 有权
标题翻译：使用编码的微载体的基因组选择和测序
公开(公告)号：US08932811B2
公开(公告)日：2015-01-13
申请号：US13203637
申请日：2010-02-26
申请人： Anja Van De Stolpe , Jacob Marinus Jan Den Toonder , Pieter Jan Van Der Zaag
发明人： Anja Van De Stolpe , Jacob Marinus Jan Den Toonder , Pieter Jan Van Der Zaag
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/00 , C12M1/34 , B32B5/16 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6869 , Y10T428/2982 , C12Q2565/519 , C12Q2563/149 , C12Q2537/143
摘要： The present invention relates to a method for determining the sequence of a nucleic molecule. Herein a capture oligonucleotide probe is attached to an encoded microcarrier, wherein the code of said microcarrier identifies the sequence of said oligonucleotide probe. The capture oligonucleotide probe is hybridized with a sample comprising nucleic acids molecules, wherein said DNA fragment comprises a sequence which is complementary to the sequence of the capture oligonucleotide probe. The sequence of the DNA molecule is determined, wherein the capture oligonucleotide probe serves as a primer for a DNA polymerase, in the case of single molecule sequencing this is a sequencing primer. After the sequence determination, the nucleotide sequence of the capture oligonucleotide probe is identified by determining the code on the microcarrier, which corresponds with the capture oligonucleotide probe. This sequence information directly identifies the location of the sequenced DNA fragment on the genome, allowing direct comparison.
摘要翻译：本发明涉及确定核酸分子序列的方法。本文中，捕获寡核苷酸探针连接到编码的微载体上，其中所述微载体的编码识别所述寡核苷酸探针的序列。捕获寡核苷酸探针与包含核酸分子的样品杂交，其中所述DNA片段包含与捕获寡核苷酸探针的序列互补的序列。确定DNA分子的序列，其中捕获寡核苷酸探针用作DNA聚合酶的引物，在单分子测序的情况下，这是测序引物。序列测定后，通过测定与捕获寡核苷酸探针对应的微载体上的编码来鉴定捕获寡核苷酸探针的核苷酸序列。该序列信息直接识别基因组上测序的DNA片段的位置，从而直接比较。

7. 发明申请

US20110311975A1 GENOMIC SELECTION AND SEQUENCING USING ENCODED MICROCARRIERS 有权
标题翻译：使用编码微控制器的基因选择和序列
公开(公告)号：US20110311975A1
公开(公告)日：2011-12-22
申请号：US13203637
申请日：2010-02-26
申请人： Anja Van De Stolpe , Jacob Marinus Jan Den Toonder , Pieter Jan Van Der Zaag
发明人： Anja Van De Stolpe , Jacob Marinus Jan Den Toonder , Pieter Jan Van Der Zaag
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6869 , Y10T428/2982 , C12Q2565/519 , C12Q2563/149 , C12Q2537/143
摘要： The present invention relates to a method for determining the sequence of a nucleic molecule. Herein a capture oligonucleotide probe is attached to an encoded microcarrier, wherein the code of said microcarrier identifies the sequence of said oligonucleotide probe. The capture oligonucleotide probe is hybridized with a sample comprising nucleic acids molecules, wherein said DNA fragment comprises a sequence which is complementary to the sequence of the capture oligonucleotide probe. The sequence of the DNA molecule is determined, wherein the capture oligonucleotide probe serves as a primer for a DNA polymerase, in the case of single molecule sequencing this is a sequencing primer. After the sequence determination, the nucleotide sequence of the capture oligonucleotide probe is identified by determining the code on the microcarrier, which corresponds with the capture oligonucleotide probe. This sequence information directly identifies the location of the sequenced DNA fragment on the genome, allowing direct comparison.
摘要翻译：本发明涉及确定核酸分子序列的方法。本文中，捕获寡核苷酸探针连接到编码的微载体上，其中所述微载体的编码识别所述寡核苷酸探针的序列。捕获寡核苷酸探针与包含核酸分子的样品杂交，其中所述DNA片段包含与捕获寡核苷酸探针的序列互补的序列。确定DNA分子的序列，其中捕获寡核苷酸探针用作DNA聚合酶的引物，在单分子测序的情况下，这是测序引物。序列测定后，通过测定与捕获寡核苷酸探针对应的微载体上的编码来鉴定捕获寡核苷酸探针的核苷酸序列。该序列信息直接识别基因组上测序的DNA片段的位置，从而直接比较。

8. 发明申请

US20110108423A1 NANOPORE DEVICE AND A METHOD FOR NUCLEIC ACID ANALYSIS 有权
标题翻译：纳米装置和核酸分析方法
公开(公告)号：US20110108423A1
公开(公告)日：2011-05-12
申请号：US13003308
申请日：2009-02-17
申请人： Pieter Jan Van Der Zaag , Anja Van De Stolpe , Elaine Mccoo , Eva Van Van Wanrooij
发明人： Pieter Jan Van Der Zaag , Anja Van De Stolpe , Elaine Mccoo , Eva Van Van Wanrooij
IPC分类号： G01N27/447 , H05K13/00 , B82Y5/00
CPC分类号： G01N33/48721
摘要： A nanopore device is described wherein is provided a sample input (110), an input chamber (120), and first and second sample chambers (130, 140) connected to the input chambers (120) via first and second nanopores (135, 145).
摘要翻译：描述了纳米孔装置，其中提供了样品输入（110），输入室（120）以及通过第一和第二纳米孔（135,145）连接到输入室（120）的第一和第二样品室（130,140））。

9. 发明授权

US08784623B2 Nanopore device and a method for nucleic acid analysis 有权
标题翻译：纳米装置和核酸分析方法
公开(公告)号：US08784623B2
公开(公告)日：2014-07-22
申请号：US13003308
申请日：2009-02-17
申请人： Pieter Jan Van Der Zaag , Anja Van De Stolpe , Elaine McCoo , Eva Van Van Wanrooij
发明人： Pieter Jan Van Der Zaag , Anja Van De Stolpe , Elaine McCoo , Eva Van Van Wanrooij
IPC分类号： G01N27/327
CPC分类号： G01N33/48721
摘要： A nanopore device is described wherein is provided a sample input (110), an input chamber (120), and first and second sample chambers (130, 140) connected to the input chambers (120) via first and second nanopores (135, 145).
摘要翻译：描述了纳米孔装置，其中提供了样品输入（110），输入室（120）以及通过第一和第二纳米孔（135,145）连接到输入室（120）的第一和第二样品室（130,140））。

10. 发明申请

US20130226621A1 IN VITRO DIAGNOSTIC TESTING INCLUDING AUTOMATED BROKERING OF ROYALTY PAYMENTS FOR PROPRIETARY TESTS 审中-公开
标题翻译：在体外诊断测试中，包括自动检测专有费用的专有费用
公开(公告)号：US20130226621A1
公开(公告)日：2013-08-29
申请号：US13881512
申请日：2011-10-24
申请人： Pieter Jan Van Der Zaag , Nevenka Dimitrova , Anja Van De Stolpe , Hendrik Van Houten
发明人： Pieter Jan Van Der Zaag , Nevenka Dimitrova , Anja Van De Stolpe , Hendrik Van Houten
IPC分类号： G06Q20/10 , G06Q50/22
CPC分类号： G06Q20/10 , G06Q10/00 , G06Q50/22 , G16B20/00 , G16H40/20 , G16H40/63
摘要： A method comprises: recording an electronic record of diagnostic events including the performing of in-vitro diagnostic tests; during the recording, identifying the performing of a licensed in-vitro diagnostic test for which license information is stored in a licenses database; and computing license compensation due to a licensor for the performing of the licensed in-vitro diagnostic test based on royalty calculation information stored in the licenses database. The recording, identifying, and computing operations are performed by one or more computers. The method may further include remitting the computed license compensation to the licensor, the remitting also being performed by one or more computers. The licensed in-vitro diagnostic test may comprise a licensed biomarker test, for example one obtained by DNA or RNA sequencing, and the method may further comprise: performing the licensed biomarker test using a genome sequencer, wherein the performing of the licensed biomarker test and the computing of license compensation are both performed at a single medical facility.
摘要翻译：一种方法包括：记录诊断事件的电子记录，包括进行体外诊断测试; 在记录期间，识别许可证体验诊断测试的执行，许可信息被存储在许可证数据库中; 以及根据许可人根据存储在许可证数据库中的版税计算信息执行许可的体外诊断测试来计算许可证补偿。记录，识别和计算操作由一个或多个计算机执行。该方法还可以包括将所计算的许可证补偿递交给许可方，还由一个或多个计算机执行。许可的体外诊断测试可以包括许可的生物标志物测试，例如通过DNA或RNA测序获得的测试，并且该方法还可以包括：使用基因组测序仪进行许可的生物标志物测试，其中执行许可的生物标志物测试和许可证补偿的计算都在单一医疗机构进行。

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