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1. 发明授权

US08916383B2 Apparatus and methods to gain access to and extract intact immature embryos from developing maize kernels or specific internal tissue or structures from one or more seeds 有权
标题翻译：从一个或多个种子开发玉米粒或特定内部组织或结构的获取和提取完整的未成熟胚的装置和方法
公开(公告)号：US08916383B2
公开(公告)日：2014-12-23
申请号：US12853997
申请日：2010-08-10
申请人： Roberto Barreiro , Jason M. Cope , Daniel M. Goldman , James L. Hunter , Alessandro Pellegrineschi
发明人： Roberto Barreiro , Jason M. Cope , Daniel M. Goldman , James L. Hunter , Alessandro Pellegrineschi
IPC分类号： C12N5/00 , C12N5/02 , A01H1/04 , A01H4/00 , G06Q10/06
CPC分类号： A01H4/001 , A01H1/04 , A01H4/003 , G06Q10/0637
摘要： A high throughput apparatus and method for gaining access to and extracting internal tissue or structure of interest from a seed or plurality of seed is disclosed. In one aspect, an apparatus utilizes means to expose the internal tissue or structure and means to separate at least some of the exposed internal tissue or structure for collection and evaluation. In one aspect, a method utilizes some force or action to expose the internal tissue or structure and some force or action to separate the internal tissue or structure.
摘要翻译：公开了一种用于从种子或多个种子获取和提取感兴趣的内部组织或结构的高通量装置和方法。在一个方面，一种装置利用暴露内部组织或结构的装置以及用于分离至少一些暴露的内部组织或结构以用于收集和评价的装置。在一个方面，一种方法利用一些力或作用来暴露内部组织或结构以及一些分离内部组织或结构的力或作用。

2. 发明授权

US08523092B2 System and method for creating a test sample from individual seeds or tissue structures 有权
标题翻译：用于从单独的种子或组织结构产生测试样品的系统和方法
公开(公告)号：US08523092B2
公开(公告)日：2013-09-03
申请号：US12880489
申请日：2010-09-13
申请人： Jason M. Cope , David Kurth
发明人： Jason M. Cope , David Kurth
IPC分类号： B02C19/00
CPC分类号： G01N1/286
摘要： The present invention is generally directed to a system and method for obtaining a test sample from a seed or seeds. The method generally includes placing at least one seed into a seed sampling chamber, breaking the seed into a plurality of seed particles inside the seed sampling chamber, placing a sampling element into the seed sampling chamber, and collecting at least a portion of the plurality of seed particles with the sampling element by attracting at least a portion of the seed particles to a surface of the sampling element or by forcing at least a portion of the seed particles into a feature of the sampling element. In some embodiments, the method further includes using a sampling element transfer device to transfer the sampling element to an extraction well, which may be used to extract DNA or proteins from the collected portion of seed particles.
摘要翻译：本发明一般涉及从种子或种子获得测试样品的系统和方法。该方法通常包括将至少一个种子放置在种子取样室中，将种子分解成种子采样室内的多个种子颗粒，将采样元件放置到种子取样室中，并收集多个通过将至少一部分种子颗粒吸引到采样元件的表面或通过迫使至少一部分种子颗粒成为采样元件的特征而将采样元件与种子颗粒接触。在一些实施例中，该方法还包括使用采样元件转移装置将采样元件转移到提取井，其可用于从收集的种子颗粒部分提取DNA或蛋白质。

3. 发明申请

US20120315377A1 APPARATUS, SYSTEM, AND METHOD FOR AUTOMATED DISPENSING OF A SUBSTANCE ON ONE OR MORE SEEDS 审中-公开
标题翻译：用于自动分配一种或多种种子的物质的装置，系统和方法
公开(公告)号：US20120315377A1
公开(公告)日：2012-12-13
申请号：US13156901
申请日：2011-06-09
申请人： Amy D. Blahnik , Jason M. Cope , Bradley T. McAvoy , Gang Wan
发明人： Amy D. Blahnik , Jason M. Cope , Bradley T. McAvoy , Gang Wan
IPC分类号： A01N3/00 , B05C5/00
CPC分类号： A01C1/06
摘要： Embodiments of the present invention provide automated apparatus, systems, and methods for dispensing a substance on a seed. An example embodiment provides a system for automated dispensing of a substance on a seed. The system comprises a seed container with a plurality of compartments each configured to retain a seed. The system further comprises a supply tank configured to retain the substance. The system also comprises at least one dispensing tip configured to dispense the substance and a pump connected to the supply tank and to the dispensing tip. The pump is configured to pump the substance from the supply tank to the dispensing tip. The system further comprises a controller configured to move at least one of the seed container or the dispensing tip relative to the other and to direct the pump to pump the substance into a compartment of the seed container.
摘要翻译：本发明的实施例提供了用于在种子上分配物质的自动化装置，系统和方法。一个示例性实施例提供了一种用于在种子上自动分配物质的系统。该系统包括具有多个隔室的种子容器，每个隔间被配置为保持种子。该系统还包括配置成保持物质的供应罐。该系统还包括构造成分配物质的至少一个分配尖端和连接到供应罐和分配尖端的泵。泵被配置为将物质从供应罐泵送到分配尖端。该系统还包括控制器，该控制器被配置为相对于另一种移动种子容器或分配尖端中的至少一个并且引导泵将物质泵送到种子容器的隔室中。

4. 发明申请

US20120126554A1 APPARATUS, METHOD AND SYSTEM FOR SIMULTANEOUSLY PICKING UP AND RELEASING OBJECTS IN BULK 审中-公开
标题翻译：同时采集和释放大容量物体的装置，方法和系统
公开(公告)号：US20120126554A1
公开(公告)日：2012-05-24
申请号：US13115640
申请日：2011-05-25
申请人： Steven M. Becker , Bruce E. Cadwell , Jason M. Cope , Gary L. Jaehnel , David Kurth
发明人： Steven M. Becker , Bruce E. Cadwell , Jason M. Cope , Gary L. Jaehnel , David Kurth
IPC分类号： B66C1/02 , B66C1/04 , B66C1/00
CPC分类号： A01C1/04 , A01C7/02 , A01C7/04 , B25J1/00 , B65B5/08
摘要： A seed handling tool includes a handle connected to a working body. The working body includes a working surface, which further includes a plurality of seed retention points. A seed retention source is provided to allow the seed handling tool to pick up and retain seeds at the seed retention points. In some embodiments, the seeds may also be released from the seed retention points. A method for retaining a plurality of seeds by the seed handling tool includes moving the seed handling tool to a position where the working surface is adjacent to a plurality of seeds, and picking up and retaining seeds at a plurality of seed retention points fixed at the working surface. In some embodiments, the method may also include releasing the plurality of seeds from the seed retention points.
摘要翻译：种子处理工具包括连接到工作体的手柄。工作体包括工作表面，其还包括多个种子保留点。提供种子保留源以允许种子处理工具拾取并保留种子保留点处的种子。在一些实施方案中，种子也可以从种子保留点释放。用于通过种子处理工具保持多个种子的方法包括将种子处理工具移动到工作表面与多个种子相邻的位置，以及在固定在所述种子处理工具的多个种子保留点上拾取并保留种子工作面在一些实施例中，该方法还可以包括从种子保留点释放多个种子。

5. 发明申请

US20110297589A1 APPARATUS AND METHOD FOR SEED SELECTION 有权
标题翻译：种子选择的装置和方法
公开(公告)号：US20110297589A1
公开(公告)日：2011-12-08
申请号：US13113673
申请日：2011-05-23
申请人： Steven M. Becker , Jason M. Cope , James Dimond , Joshua L. Mongan
发明人： Steven M. Becker , Jason M. Cope , James Dimond , Joshua L. Mongan
IPC分类号： B07C5/36 , B07C5/34
CPC分类号： G01N1/286 , A01C1/00 , A01H1/04 , B07C5/36 , G01N35/0099 , G01N2033/0077
摘要： Apparatuses and methods for selecting one or more seeds from a group of seeds based on a predetermined characteristic of the selected seeds are provided. In various embodiments, the seeds are selected from multiple containers of seeds and re-configured into a single container for later use. The apparatus includes an array of seed selectors that expel the selected seeds from the corresponding compartments through contact with the compartments. A transfer member then directs each expelled seed to a cavity of a collection tray or into an envelope or other receptacle. A controller may run operation of the apparatus and associate the selected seeds with the cavities and/or receptacle into which they are deposited. In this way, numerous seeds may be automatically selected substantially simultaneously or in rapid succession and re-configured in a short period of time and with limited user intervention.
摘要翻译：提供了基于所选种子的预定特征从一组种子中选择一种或多种种子的装置和方法。在各种实施方案中，种子选自多个种子容器，并重新配置成单个容器以备后用。该装置包括种子选择器的阵列，其通过与隔室接触而将所选种子从相应的隔间排出。转移构件然后将每个排出的种子引导到收集盘的空腔或者封入或其它容器中。控制器可以运行设备的操作并将所选择的种子与其所沉积的空腔和/或插座相关联。以这种方式，可以基本上同时或快速连续地自动选择许多种子并且在短时间内并且在有限的用户干预下重新配置。

6. 发明授权

US07141260B2 Apparatus and method for removal of seed pericarp 有权
标题翻译：用于去除种子果皮的装置和方法
公开(公告)号：US07141260B2
公开(公告)日：2006-11-28
申请号：US10230688
申请日：2002-08-29
申请人： Jason M. Cope , Wade Yang , Billy Davidson
发明人： Jason M. Cope , Wade Yang , Billy Davidson
IPC分类号： A23L1/025
CPC分类号： A23N5/00
摘要： An apparatus and method for removing pericarp from a seed, such as a corn kernel. The method includes sonicating the kernel to loosen the pericarp and then separation of the pericarp. One example of separation is by frictional milling. An additional optional aspect of the invention is isolation of the pericarp from the remainder of the seed and/or further cleaning or purification of the pericarp.
摘要翻译：用于从种子（例如玉米粒）中除去果皮的装置和方法。该方法包括超声处理核以松开果皮，然后分离果皮。分离的一个例子是摩擦磨。本发明的另外的任选方面是将果皮与种子的其余部分分离和/或进一步清洁或纯化果皮。

7. 发明授权

US08833565B2 Apparatus and method for seed selection 有权
标题翻译：种子选择的装置和方法
公开(公告)号：US08833565B2
公开(公告)日：2014-09-16
申请号：US13113673
申请日：2011-05-23
申请人： Steven M. Becker , Jason M. Cope , James Dimond , Joshua L. Mongan
发明人： Steven M. Becker , Jason M. Cope , James Dimond , Joshua L. Mongan
IPC分类号： B07C5/34 , G01N35/00 , A01H1/04 , A01C1/00 , B07C5/36 , G01N1/28 , G01N33/00
CPC分类号： G01N1/286 , A01C1/00 , A01H1/04 , B07C5/36 , G01N35/0099 , G01N2033/0077
摘要： Apparatuses and methods for selecting one or more seeds from a group of seeds based on a predetermined characteristic of the selected seeds are provided. In various embodiments, the seeds are selected from multiple containers of seeds and re-configured into a single container for later use. The apparatus includes an array of seed selectors that expel the selected seeds from the corresponding compartments through contact with the compartments. A transfer member then directs each expelled seed to a cavity of a collection tray or into an envelope or other receptacle. A controller may run operation of the apparatus and associate the selected seeds with the cavities and/or receptacle into which they are deposited. In this way, numerous seeds may be automatically selected substantially simultaneously or in rapid succession and re-configured in a short period of time and with limited user intervention.
摘要翻译：提供了基于所选种子的预定特征从一组种子中选择一种或多种种子的装置和方法。在各种实施方案中，种子选自多个种子容器，并重新配置成单个容器以备后用。该装置包括种子选择器的阵列，其通过与隔室接触而将所选种子从相应的隔间排出。转移构件然后将每个排出的种子引导到收集盘的空腔或者封入或其它容器中。控制器可以运行设备的操作并将所选择的种子与其所沉积的空腔和/或插座相关联。以这种方式，可以基本上同时或快速连续地自动选择许多种子并且在短时间内并且在有限的用户干预下重新配置。

8. 发明授权

US08313053B2 High throughput, seed sampling and collection system and method 有权
标题翻译：高通量，种子采样和收集系统和方法
公开(公告)号：US08313053B2
公开(公告)日：2012-11-20
申请号：US12726874
申请日：2010-03-18
申请人： Jason M. Cope , Gary L. Jaehnel , Joshua L. Mongan
发明人： Jason M. Cope , Gary L. Jaehnel , Joshua L. Mongan
IPC分类号： B02C15/00
CPC分类号： G01N1/286 , G01N35/00 , G01N2001/2866
摘要： A system and method for preparing a sample of seeds or representative seed portions are provided. In various embodiments, the system and method include a force applying member and a seed container that includes at least one compartment containing a seed. The force applying member is configured to apply a force to the seed so as to break the seed into two or more seed particles, which in some embodiments may be collected in a seed particle collector. The present invention improves on the prior art by greatly reducing (and in some embodiments eliminating) the manual processes typically involved in generating tissue samples from seeds and preparing the tissue for genetic analysis. Additionally, the present invention is scaleable, and can be configured to generate samples from many seeds in a short period of time. The present invention also minimizes the risk of contamination and cross-contamination of the seed particles.
摘要翻译：提供了一种用于制备种子样品或代表种子部分的系统和方法。在各种实施例中，系统和方法包括施力构件和种子容器，其包括至少一个包含种子的隔室。力施加构件构造成向种子施加力，以便将种子分解成两个或更多种子颗粒，其在一些实施方案中可以收集在种子颗粒收集器中。本发明通过大大减少（并且在一些实施例中消除）通常涉及从种子产生组织样品并制备用于遗传分析的组织的手工过程来改进现有技术。此外，本发明是可扩展的，并且可以被配置为在短时间内从许多种子生成样本。本发明还使种子颗粒的污染和交叉污染的风险最小化。

9. 发明申请

US20110160068A1 AUTOMATED SEED SAMPLING APPARATUS, METHOD AND SYSTEM 有权
标题翻译：自动种子采样设备，方法与系统
公开(公告)号：US20110160068A1
公开(公告)日：2011-06-30
申请号：US12981954
申请日：2010-12-30
申请人： Steven M. Becker , Jason M. Cope , David Kurth , Joshua L. Mongan
发明人： Steven M. Becker , Jason M. Cope , David Kurth , Joshua L. Mongan
IPC分类号： C40B30/00 , C40B60/12
CPC分类号： G01N1/04 , G01N35/028 , G01N35/1065 , G01N2001/045 , G01N2033/0077
摘要： An automated high throughput apparatus, method and system for seed sampling is provided. The seed sampling system includes an ablation subsystem having a controlled cutting path and a seed handling subsystem comprising a staging system having a plurality of seed staging positions for staging a batch of seed in a sequential manner, and a carrier system for simultaneously presenting seed staging positions at the cutting path for removing a sample portion from each seed in the batch. A method of this invention includes providing ablation means having a controlled cutting path, automatically staging a batch of seed at a plurality of staging positions in a sequential manner, simultaneously presenting the batch of seed at the cutting path, and removing a sample portion from each seed in the batch.
摘要翻译：提供了一种用于种子取样的自动化高通量装置，方法和系统。种子采样系统包括具有受控切割路径的消融子系统和种子处理子系统，其包括具有多个种子分级位置的分级系统，用于以顺序方式分级一批种子;以及载体系统，用于同时呈现种子分期位置在切割路径上用于从批次中的每个种子中除去样品部分。本发明的方法包括提供具有受控切割路径的消融装置，以顺序方式在多个分段位置自动分级一批种子，同时在切割路径上呈现一批种子，以及从每个处移除样品部分种子在批量。

10. 发明授权

US08863436B2 Automated seed sampling apparatus, method and system 有权
标题翻译：自动种子取样装置，方法和系统
公开(公告)号：US08863436B2
公开(公告)日：2014-10-21
申请号：US12981954
申请日：2010-12-30
申请人： Steven M. Becker , Jason M. Cope , David Kurth , Joshua L. Mongan
发明人： Steven M. Becker , Jason M. Cope , David Kurth , Joshua L. Mongan
IPC分类号： A01C1/00 , G01N33/00 , G01N1/04 , G01N35/02 , G01N35/10
CPC分类号： G01N1/04 , G01N35/028 , G01N35/1065 , G01N2001/045 , G01N2033/0077
摘要： An automated high throughput apparatus, method and system for seed sampling is provided. The seed sampling system includes an ablation subsystem having a controlled cutting path and a seed handling subsystem comprising a staging system having a plurality of seed staging positions for staging a batch of seed in a sequential manner, and a carrier system for simultaneously presenting seed staging positions at the cutting path for removing a sample portion from each seed in the batch. A method of this invention includes providing ablation means having a controlled cutting path, automatically staging a batch of seed at a plurality of staging positions in a sequential manner, simultaneously presenting the batch of seed at the cutting path, and removing a sample portion from each seed in the batch.
摘要翻译：提供了一种用于种子取样的自动化高通量装置，方法和系统。种子采样系统包括具有受控切割路径的消融子系统和种子处理子系统，其包括具有多个种子分级位置的分级系统，用于以顺序方式分级一批种子;以及载体系统，用于同时呈现种子分期位置在切割路径上用于从批次中的每个种子中除去样品部分。本发明的方法包括提供具有受控切割路径的消融装置，以顺序方式在多个分段位置自动分级一批种子，同时在切割路径上呈现一批种子，以及从每个处移除样品部分种子在批量。

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