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1. 发明授权

US06355467B1 Saccharification enzymes from hyperthermophilic bacteria and processes for their production 失效
标题翻译：来自超嗜热菌的糖化酶及其生产过程
公开(公告)号：US06355467B1
公开(公告)日：2002-03-12
申请号：US09503335
申请日：2000-02-14
申请人： Robert M. Kelly , Stephen H. Brown , Henry R. Costantino
发明人： Robert M. Kelly , Stephen H. Brown , Henry R. Costantino
IPC分类号： C12N928
CPC分类号： C12N9/2411 , C12N9/2408 , C12N9/2417 , C12N9/2451 , C12N9/2457 , C12Y302/0101 , C12Y302/0102 , C12Y302/01041
摘要： It has been found that Pyrococcus furiosus produces several starch hydrolyzing enzymes, both extracellularly and intracellularly, which retain their activity for several hours at or above 100° C. These comprise an &agr;-glucosidase, which has been highly purified, and amylolytic enzymes including an amylase and a pullulanase. In addition, at least one thermostable enzyme activity from P. furiosus that is useful in the process of degradation of cellulose to D-glucose has also been found, namely a &bgr;-glucosidase, which is also active up to at least 95° C.
摘要翻译：已经发现，激烈热球菌在细胞外和细胞内产生几种淀粉水解酶，其在100℃或100℃以上保持其活性几个小时。这些包括高度纯化的α-葡糖苷酶和淀粉分解酶，包括淀粉酶和支链淀粉酶。此外，还发现了可用于将纤维素降解为D-葡萄糖的过程中来自激素抗原的至少一种热稳定酶活性，即β-葡糖苷酶，其在高达至少95℃时也是有效的。

2. 发明授权

US5268280A Method for glucose isomerization using xylose isomerase purified from Thermotoga Maritima and Thermotoga Neapolitana 失效
标题翻译：使用从Thermotoga Maritima和Thermotoga Neapolitana纯化的木糖异构酶的葡萄糖异构化方法
公开(公告)号：US5268280A
公开(公告)日：1993-12-07
申请号：US47591
申请日：1993-04-13
申请人： Robert L. Starnes , Robert M. Kelly , Stephen H. Brown
发明人： Robert L. Starnes , Robert M. Kelly , Stephen H. Brown
IPC分类号： C12N9/92 , C12P19/24 , C12N9/58 , C12N11/00
CPC分类号： C12N9/92 , C12P19/24
摘要： This invention is in the field of glucose isomerization enzymes. More specifically, the invention is directed to a novel xylose isomerase, a process for the preparation of this enzyme, the use of this enzyme in glucose isomerization processes, and glucose isomerization processes.The enzyme is preferrably derived from Thermotoga maritima or Thermotoga neapolitana. The enzyme has a temperature optimum above 90.degree. C., pH optimum in the range of from 6 to 7 and a residual activity at 90.degree. C. of more than 40% after 30 minutes and/or residual activity at 98.degree. C. of more than 20% after 30 minutes. The enzyme can also be in immobilized form.
摘要翻译：本发明属于葡萄糖异构化酶领域。更具体地说，本发明涉及一种新型的木糖异构酶，一种制备该酶的方法，该酶在葡萄糖异构化方法中的应用以及葡萄糖异构化方法。该酶优选地来源于海参Thermotoga或Thermotoga neapolitana。酶的最适温度在90℃以上，pH最适于6〜7，90℃下的残留活性在30分钟后超过40％，和/或98℃的残留活性 30分钟后超过20％。酶也可以是固定化形式。

3. 发明授权

US5242817A Proteolytic enzymes from hyperthermophilic bacteria and processes for their production 失效
标题翻译：来自超嗜热细菌的蛋白水解酶及其生产过程
公开(公告)号：US5242817A
公开(公告)日：1993-09-07
申请号：US406327
申请日：1989-09-12
申请人： Robert M. Kelly , Anne K. S. Robinson , Ilse I. Blumentals , Stephen H. Brown , Christian B. Anfinsen
发明人： Robert M. Kelly , Anne K. S. Robinson , Ilse I. Blumentals , Stephen H. Brown , Christian B. Anfinsen
IPC分类号： C12N9/52
CPC分类号： C12N9/52
摘要： Cell-free extracts from Pyrococcus furiosus were found to possess unusually high levels of proteolytic activity as measured by hydrolysis of azocasein; loss in activity was only 30% after incubation for 24 hours at 98.degree. C. and the half-life of proteolytic activity at that temperature was about 60 hours. Furthermore, cell-free extracts incubated at 98.degree. C. in 1% sodium dodecyl sulfate (SDS) for 24 hours yielded an SDS-resistant protease having a temperature optimum of at least 100.degree. C. The enzyme retained at least 40% of its activity when tested at 98.degree. C. by azocasein hydrolysis in the presence of 4M urea, 2M guanidinium chloride, 10 mM dithiothreitol or 150 mM .beta.-mercaptoethanol. The protease was found to have a pH optimum of 6.8 at 98.degree. C. and retained more than 45% of its activity at pH 9.3 and 82% of its activity at pH 4.5 in assays performed at those values. The protease was classified as a metalloprotease through inhibitor studies, and peptide hydrolysis showed trypsin-like cleavage with additional activities.
摘要翻译：发现激烈热球菌的无细胞提取物通过偶氮酪蛋白水解测定具有异常高水平的蛋白水解活性; 在98℃孵育24小时后，活性损失仅为30％，在该温度下蛋白水解活性的半衰期为约60小时。此外，在98℃，1％十二烷基硫酸钠（SDS）中孵育24小时的无细胞提取物产生温度最佳为至少100℃的SDS-抗性蛋白酶。酶保留其至少40％当在4M尿素，2M氯化胍胍，10mM二硫苏糖醇或150mMβ-巯基乙醇存在下，通过偶氮酪蛋白水解在98℃下测试活性。发现蛋白酶在98℃下的pH最佳值为6.8，并且在这些值下进行的分析中，在pH9.3下保留其活性高于45％，其pH值为4.5的活性为82％。蛋白酶通过抑制剂研究被分类为金属蛋白酶，肽水解显示胰蛋白酶样切割具有额外的活性。

4. 发明授权

US5391489A Proteolytic enzymes from hyperthermophilic bacteria and processes for their production 失效
标题翻译：来自超嗜热细菌的蛋白水解酶及其生产过程
公开(公告)号：US5391489A
公开(公告)日：1995-02-21
申请号：US83536
申请日：1993-06-30
申请人： Robert M. Kelly , Anne K. S. Robinson , Ilse I. Blumentals , Stephen H. Brown , Christian B. Anfinsen
发明人： Robert M. Kelly , Anne K. S. Robinson , Ilse I. Blumentals , Stephen H. Brown , Christian B. Anfinsen
IPC分类号： C12N9/52 , C12P21/04 , C12N9/50
CPC分类号： C12N9/52
摘要： Cell-free extracts from Pyrococcus furiosus were found to possess unusually high levels of proteolytic activity as measured by hydrolysis of azocasein; loss in activity was only 30% after incubation for 24 hours at 98.degree. C. and the half-life of proteolytic activity at that temperature was about 60 hours. Furthermore, cell-free extracts incubated at 98.degree. C. in 1% sodium dodecyl sulfate (SDS) for 24 hours yielded an SDS-resistant protease having a temperature optimum of at least 100.degree. C. The enzyme retained at least 40% of its activity when tested at 98.degree. C. by azocasein hydrolysis in the presence of 4M urea, 2M guanidinium chloride, 10 mM dithiothreitol or 150 mM .beta.-mercaptoethanol. The protease was found to have a pH optimum of 6.8 at 98.degree. C. and retained more than 45% of its activity at pH 9.3 and 82% of its activity at pH 4.5 in assays performed at those values. The protease was classified as a metalloprotease through inhibitor studies, and peptide hydrolysis showed trypsin-like cleavage with additional activities.
摘要翻译：发现激烈热球菌的无细胞提取物通过偶氮酪蛋白水解测定具有异常高水平的蛋白水解活性; 在98℃孵育24小时后，活性损失仅为30％，在该温度下蛋白水解活性的半衰期为约60小时。此外，在98℃，1％十二烷基硫酸钠（SDS）中孵育24小时的无细胞提取物产生温度最佳为至少100℃的SDS-抗性蛋白酶。酶保留其至少40％当在4M尿素，2M氯化胍胍，10mM二硫苏糖醇或150mMβ-巯基乙醇存在下，通过偶氮酪蛋白水解在98℃下测试活性。发现蛋白酶在98℃下的pH最佳值为6.8，并且在这些值下进行的分析中，在pH9.3下保留其活性高于45％，其pH值为4.5的活性为82％。蛋白酶通过抑制剂研究被分类为金属蛋白酶，肽水解显示胰蛋白酶样切割具有额外的活性。

5. 发明授权

US5219751A Xylase isomerase purified from Thermotoga maritima and Thermotoga Neapolitana 失效
标题翻译：从THERMOTOGA MARITIMA和THERMOTOGA NEAPOLITANA纯化的XYLASE异构体
公开(公告)号：US5219751A
公开(公告)日：1993-06-15
申请号：US600544
申请日：1990-10-19
申请人： Robert L. Starnes , Robert M. Kelly , Stephen H. Brown
发明人： Robert L. Starnes , Robert M. Kelly , Stephen H. Brown
IPC分类号： C12N9/90 , C12N9/92 , C12P19/24 , C12R1/01
CPC分类号： C12N9/92 , C12P19/24 , Y10S435/822
摘要： This invention is in the field of glucose isomerization enzymes. More specifically, the invention is directed to a novel xylose isomerase, a process for the preparation of this enzyme, the use of this enzyme in glucose isomerization processes, and glucose isomerization processes. The enzyme is preferably derived from Thermotoga maritima or Thermotoga neapolitana. The enzyme has a temperature optimum above 90.degree. C., pH optimum in the range of from 6 to 7 and a residual activity at 90.degree. C. of more than 40% after 30 minutes and/or residual activity at 98.degree. C. of more than 20% after 30 minutes. The enzyme can also be in immobilized form.
摘要翻译：本发明属于葡萄糖异构化酶领域。更具体地说，本发明涉及一种新型的木糖异构酶，一种制备该酶的方法，该酶在葡萄糖异构化方法中的应用以及葡萄糖异构化方法。该酶优选衍生自海岸假单胞菌（Thermotoga maritima）或海芋（Thermotoga neapolitana）。酶的最适温度在90℃以上，pH最适于6〜7，90℃下的残留活性在30分钟后超过40％，和/或98℃的残留活性 30分钟后超过20％。酶也可以是固定化形式。

6. 发明授权

US08668406B2 Subsea cable installation 有权
标题翻译：海底电缆安装
公开(公告)号：US08668406B2
公开(公告)日：2014-03-11
申请号：US09988821
申请日：2001-11-20
申请人： Ronald J. Vidal , Paul Alexander , Robert M. Kelly , David Bruton , Robert Todd
发明人： Ronald J. Vidal , Paul Alexander , Robert M. Kelly , David Bruton , Robert Todd
IPC分类号： F16L1/16
CPC分类号： H02G15/14 , F16L1/123 , G02B6/506 , H02G1/10
摘要： The invention facilitates provision of a point-to-point cable connection between first and second points separated by an extended span of water including a first region of shallow water and a second region of relatively deep water. A plurality of ducts are provided from the first point through the first region of the extended span to an offshore termination point between the first and second points. Preferably, the ducts are buried in the seabed to prevent damage. At least one first cable is placed in one of the plurality of ducts to provide a connection between the first point and the offshore termination point. A second cable from the second point is received at the offshore termination point. The first cable is connected to the second cable at the offshore termination point to create the point-to-point cable connection. In one embodiment, the offshore termination point is located on the continental shelf near the transition to deep water. Cable can be installed between the first point and the offshore termination point with relative ease by passing the cable through an available conduit. This eliminates the need for the time consuming and expensive permitting process typically associated with near-shore cable installation.
摘要翻译：本发明有助于提供由包括浅水的第一区域和相对较深的水的第二区域的水的延伸跨度分开的第一和第二点之间的点对点电缆连接。多个管道从第一点延伸穿过延伸跨距的第一区域到第一和第二点之间的离岸终点。优选地，将管道埋在海床中以防止损坏。至少一个第一电缆被放置在多个管道中的一个中以提供第一点和离岸终止点之间的连接。在离岸终点处接收第二点的第二根电缆。第一根电缆连接到海上终端点处的第二根电缆，以创建点对点电缆连接。在一个实施例中，离岸终点位于靠近深水过渡附近的大陆架上。通过将电缆穿过可用导管，电缆可以相对容易地安装在第一点和海上终端点之间。这消除了对通常与近岸电缆安装相关联的耗时和昂贵的许可过程的需要。

7. 发明授权

US5544613A Foot rest for boats 失效
标题翻译：船休息
公开(公告)号：US5544613A
公开(公告)日：1996-08-13
申请号：US514453
申请日：1995-08-11
申请人： Charles S. Scarborough , Robert M. Kelly , Rodney W. Gaylor , Patrick J. Rogers
发明人： Charles S. Scarborough , Robert M. Kelly , Rodney W. Gaylor , Patrick J. Rogers
IPC分类号： B63H16/02 , B63B17/00
CPC分类号： B63H16/02
摘要： A foot rest (10) for supporting the feet of a boater in a boat such as a canoe or kayak. The foot rest (10) is movable along the length of the boat such that boaters having longer or shorter legs than others may be accommodated while requiring only one adjustment. The foot rest (10) includes a mounting rail (12), a foot support block (14), and a retainer rail (16). The mounting rail (12) is a linear member and is disposed along the longitudinal axis of the boat in front of a seat. The mounting rail (12) may be formed separately from and secured to the boat. The mounting rail (12) defines a plurality of indexing receptors (24) opening on the top side thereof for receiving cooperating indexing members (30) carried by the foot support block (14). The foot support block (14) includes a pair of foot engagement pads (26) carried at either end of a bridge (28). The indexing members (30) are configured to be closely received by and cooperate with the indexing receptors (24) defined by the mounting rail (12). Each foot engagement pad (26) defines an engagement surface (32) oriented to engage the bottom of a boater's foot. The retainer rail (16) is received by the foot support block (14) and is releasably secured to the mounting rail (12) in a conventional fashion. The top surface of the retainer rail (16) is dimensioned to be closely received between the foot engagement pads (26) such that when the position of the foot support block (14) is being adjusted, the foot support block (14) is prevented from rotating about a vertical axis such that relative alignment of the foot support block (14) is maintained with respect to the mounting rail (12).
摘要翻译：用于支撑船上诸如独木舟或皮划艇的船只脚的脚踏板（10）。脚踏板（10）可以沿着船的长度移动，使得可以容纳具有比其他的更长或更短的腿的船只，同时仅需要一个调节。脚踏板（10）包括安装导轨（12），脚支撑块（14）和保持架（16）。安装导轨（12）是线性构件，并且沿着座椅的前方的船的纵向轴线设置。安装导轨（12）可以与船形成分开并固定在船上。安装轨道（12）限定了在其顶侧上开口的多个分度接收器（24），用于接收由脚部支撑块（14）承载的配合的分度构件（30）。脚支撑块（14）包括在桥（28）的任一端承载的一对脚接合垫（26）。分度构件（30）构造成与由安装轨道（12）限定的分度接收器（24）紧密接收并配合。每个脚接合垫（26）限定定向成接合船底的底部的接合表面（32）。保持架（16）由脚支撑块（14）容纳并且以常规方式可释放地固定到安装轨道（12）。定位轨道（16）的顶表面的尺寸设计成紧密地接收在脚接合垫（26）之间，使得当脚支撑块（14）的位置被调节时，脚支撑块（14）被阻止从而围绕垂直轴线旋转，使得脚支撑块（14）的相对对准相对于安装轨道（12）保持。

8. 外观设计

USD339791S Inside connector for a communication headset system 失效
公开(公告)号：USD339791S
公开(公告)日：1993-09-28
申请号：US756363
申请日：1991-09-09
申请人： Robert E. Lucey , Robert M. Kelly
设计人： Robert E. Lucey , Robert M. Kelly

9. 发明授权

US5869435A Compositions for fracturing subterranean formations 失效
$Compositions for fracturing subterranean formations$
标题翻译：压裂地层的组成
公开(公告)号：US5869435A
公开(公告)日：1999-02-09
申请号：US403078
申请日：1995-03-13
申请人： Robert M. Kelly , Saad A. Khan , Pascal Leduc , Akash Tayal , Robert K. Prud'homme
发明人： Robert M. Kelly , Saad A. Khan , Pascal Leduc , Akash Tayal , Robert K. Prud'homme
IPC分类号： C09K8/62 , C09K8/68 , E21B43/267 , E21B43/26
CPC分类号： E21B43/267 , C09K8/62 , C09K8/685 , Y10S507/921 , Y10S507/922 , Y10S507/923
摘要： A method of fracturing a subterranean formation which surrounds a well bore comprises the steps of providing a fracturing fluid, and injecting the fracturing fluid into the well bore at a pressure sufficient to form fractures in the subterranean formation which surrounds the well bore. The pressure is then released from the fracturing fluid, after which the fluid may be removed from the well and the well placed into production. The fracturing fluid comprises an aqueous liquid, a polysaccharide soluble or dispersible in the aqueous liquid in an amount sufficient to increase the viscosity of the aqueous liquid, an enzyme breaker which degrades said polysaccharide at a temperature above 180.degree. F. Fracturing fluid compositions and enzyme breaker systems useful for carrying out the invention are also disclosed.
摘要翻译：压裂围绕井眼的地下地层的方法包括提供压裂流体的步骤，以及足以在围绕井眼的地层中形成裂缝的压力将压裂流体注入井眼。然后压力从压裂液中释放出来，然后可以将流体从井中移出并将井放入生产中。压裂流体包含含水液体，可溶于或分散在含水液体中的多糖，其量足以增加水性液体的粘度，该酶在大于180°F的温度下降解所述多糖。压裂液组合物和酶还公开了可用于实施本发明的断路器系统。

10. 外观设计

USD332601S Electrical connector for a communication headset system 失效
公开(公告)号：USD332601S
公开(公告)日：1993-01-19
申请号：US756377
申请日：1991-09-09
申请人： Robert E. Lucey , Robert M. Kelly
设计人： Robert E. Lucey , Robert M. Kelly

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