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    • 3. 发明申请
    • TEST APPARATUS, PRODUCTION METHOD THEREFOR AND TEST METHOD
    • 测试装置及其生产方法及测试方法
    • US20090061460A1
    • 2009-03-05
    • US12200335
    • 2008-08-28
    • Eloisa Lopez-CalleJuergen OberstrassWerner Siekmann
    • Eloisa Lopez-CalleJuergen OberstrassWerner Siekmann
    • G01N33/53C12M1/00
    • G01N33/558
    • Test apparatus for detecting an analyte (A) contained in a sample liquid, comprising a dry porous carrier (10), on which a selective binder (12), capable of selectively binding the analyte (A) after the carrier (10) is wetted with the sample fluid, is arranged in a reaction zone (20), wherein furthermore arranged in the reaction zone (20) are: a complex, formed by a biomarker (B) and the first complexed reporter partner of a pair of reporters, interacting to produce an optically detectable signal, of reporter enzyme (E) and reporter substrate (S), and the second, free reporter partner of the pair of reporters, wherein the biomarker (B), in terms of the selectivity of the binding capacity of the selective binder (12), is equivalent to the analyte (A) and competitive therewith and wherein binding of the selective binder (12) to the complexed biomarker (B) impedes the interaction between the complexed and the free reporter partners.
    • 用于检测包含在样品液体中的分析物(A)的测试装置,包括干燥的多孔载体(10),其上在载体(10)被润湿之后能够选择性地结合分析物(A)的选择性粘合剂(12) 与样品流体一起布置在反应区(20)中,其中还设置在反应区(20)中的是:由生物标志物(B)形成的复合物和一对记者的第一个复杂的记者伴侣,相互作用 以产生报告酶(E)和报道底物(S)的光学上可检测的信号,以及该对记者的第二个自由报道配偶体,其中生物标志物(B)根据其结合能力的选择性 选择性粘合剂(12)等同于分析物(A)并与其竞争,并且其中选择性粘合剂(12)与络合的生物标志物(B)的结合阻碍了复合的和自由的报告物配偶体之间的相互作用。