专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06627395B1 Methods for the propagation, isolation, identification, and preparation of human immunodeficiency virus type 1 (HIV-1) 失效
标题翻译：人类免疫缺陷病毒1型（HIV-1）的繁殖，分离，鉴定和制备方法
公开(公告)号：US06627395B1
公开(公告)日：2003-09-30
申请号：US08466256
申请日：1995-06-06
申请人： Luc Montagnier , Jean-Claude Chermann , Francoise Barre-Sinoussi , Francoise Brun-Vezinet , Christine Rouzioux , Willy Rozenbaum , Charles Dauguet , Jacqueline Gruest , Marie-Therese Nugeyre , Francoise Rey , Claudine Axler-Blin , Solange Chamaret , Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
发明人： Luc Montagnier , Jean-Claude Chermann , Francoise Barre-Sinoussi , Francoise Brun-Vezinet , Christine Rouzioux , Willy Rozenbaum , Charles Dauguet , Jacqueline Gruest , Marie-Therese Nugeyre , Francoise Rey , Claudine Axler-Blin , Solange Chamaret , Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
IPC分类号： C12Q170
CPC分类号： C07K16/1045 , C07K14/005 , C12N7/00 , C12N2740/16051 , C12N2740/16222 , G01N2333/16
摘要： The identification, separation, purification, and propagation of the HIV-1 virus is provided. Moreover, the preparation of antigens from HIV-1 is further provided. The identification of HIV-1 involves the purification of a virus sample from lymphocytes and contacting the sample with antibodies, which bind to HIV-1 viruses, is provided. The propagation of HIV-1 virus involves infecting uninfected T lymphocytes with the virus. Moreover, the preparation of antigens from HIV-1 involves the separation of protein components of a purified HIV-1 virus under denaturing conditions.
摘要翻译：提供HIV-1病毒的鉴定，分离，纯化和扩增。此外，还提供了来自HIV-1的抗原的制备。 HIV-1的鉴定涉及从淋巴细胞中纯化病毒样品并使样品与结合HIV-1病毒的抗体接触。 HIV-1病毒的传播涉及用病毒感染未感染的T淋巴细胞。此外，HIV-1抗原的制备涉及在变性条件下分离纯化的HIV-1病毒的蛋白质组分。

2. 发明授权

US5135864A Human Immunodeficiency Virus (HIV) associated with Acquired Immunual Deficiency Syndrome (AIDS), a diagnostic method for aids and pre-aids, and a kit therefor 失效
标题翻译：与获得性免疫缺陷综合征（AIDS）相关的人类免疫缺陷病毒（HIV），辅助和预辅助的诊断方法及其工具包
公开(公告)号：US5135864A
公开(公告)日：1992-08-04
申请号：US117937
申请日：1987-11-05
申请人： Luc Montagnier , Jean-Claude Chermann , Francoise Barre-Sinoussi , Francoise Brun-Vezinet , Christine Rouzioux , Willy Rozenbaum , Charles Dauguet , Jacqueline Gruest , Marie-Therese Nugeyre , Francoise Rey , Claudine Axler-Blin , Solange Chamaret , Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
发明人： Luc Montagnier , Jean-Claude Chermann , Francoise Barre-Sinoussi , Francoise Brun-Vezinet , Christine Rouzioux , Willy Rozenbaum , Charles Dauguet , Jacqueline Gruest , Marie-Therese Nugeyre , Francoise Rey , Claudine Axler-Blin , Solange Chamaret , Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
IPC分类号： C07K14/16 , C12N7/02
CPC分类号： C07K14/005 , C12N7/00 , C12N2740/16051 , C12N2740/16222 , Y10S435/974
摘要： Retroviruses associated with Acquired Immune Deficiency Syndrome (AIDS), including Lymphadenopathy Associated Virus (LAV), are isolated from the sera of patients afflicted with Lymphadenopathy Syndrome (LAS) or AIDS. LAV is a Human Immunodeficiency Virus (HIV). Viral extract, structural proteins and other fractions of the retrovirus immunologically recognize the sera of such patients. Immunological reaction is used to detect antibodies that specifically bind to antigenic sites of the retrovirus in samples of body fluids from patients with AIDS or risk of AIDS. A kit for in vitro assay of LAS or AIDS is provided.
摘要翻译：与获得性免疫缺陷综合征（AIDS）相关的逆转录病毒，包括淋巴结病相关病毒（LAV），从患有淋巴结病综合征（LAS）或AIDS的患者血清中分离。 LAV是人类免疫缺陷病毒（HIV）。病毒提取物，结构蛋白和其他部分的逆转录病毒免疫识别这些患者的血清。免疫反应用于检测具有艾滋病或艾滋病风险的患者体液样品中特异性结合逆转录病毒抗原位点的抗体。提供了用于LAS或AIDS的体外测定的试剂盒。

3. 发明授权

US06600023B1 Antibody directed against HIV-1 P25 antigen 失效
标题翻译：针对HIV-1 P25抗原的抗体
公开(公告)号：US06600023B1
公开(公告)日：2003-07-29
申请号：US08019297
申请日：1993-02-18
申请人： Luc Montagnier , Jean-Claude Chermann , Francoise Barre-Sinoussi , Francoise Brun-Vezinet , Christine Rouzioux , Willy Rozenbaum , Charles Dauguet , Jacqueline Gruest , Marie-Therese Nugeyre , Francoise Rey , Claudine Axler-Blin , Solange Chamaret , Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
发明人： Luc Montagnier , Jean-Claude Chermann , Francoise Barre-Sinoussi , Francoise Brun-Vezinet , Christine Rouzioux , Willy Rozenbaum , Charles Dauguet , Jacqueline Gruest , Marie-Therese Nugeyre , Francoise Rey , Claudine Axler-Blin , Solange Chamaret , Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
IPC分类号： C07K1600
CPC分类号： C07K16/1045 , C07K14/005 , C12N7/00 , C12N2740/16051 , C12N2740/16222 , G01N2333/16
摘要： Antibodies which bind with antigens of human immunodeficiency virus type 1 (HIV-1), such as Lymphadenopathy Associated Virus (LAV), are disclosed. Retroviruses associated with Acquired Immune Deficiency Syndrome (AIDS) are isolated from the sera of patients afflicted with Lymphadenopathy Syndrome (LAS) or AIDS. Viral extracts, structural proteins and other fractions of the retrovirus immunologically recognize the sera of such patients.
摘要翻译：公开了与人类免疫缺陷病毒1型（HIV-1）的抗原结合的抗体，例如淋巴结病相关病毒（LAV）。与获得性免疫缺陷综合征（AIDS）相关的逆转录病毒从患有淋巴结病综合征（LAS）或AIDS的患者的血清中分离。逆转录病毒的病毒提取物，结构蛋白和其他部分免疫识别这些患者的血清。

4. 发明授权

US4708818A Human immunodeficiency viruses associated with Acquired Immune Deficiency Syndrome (AIDS), a diagnostic method for AIDS and pre-AIDS, and a kit therefor 失效
标题翻译：与获得性免疫缺陷综合征（AIDS）相关的人体免疫机能丧失病毒，艾滋病和艾滋病前期的诊断方法及其试剂盒
公开(公告)号：US4708818A
公开(公告)日：1987-11-24
申请号：US785638
申请日：1985-10-08
申请人： Luc Montagnier , Jean-Claude Chermann , Francoise Barre-Sinoussi , Francoise Brun-Vezinet , Christine Rouzioux , Willy Rozenbaum , Charles Dauguet , Jacqueline Gruest , Marie-Therese Nugeyre , Francoise Rey , Claudine Axler-Blin , Solange Chamaret , Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
发明人： Luc Montagnier , Jean-Claude Chermann , Francoise Barre-Sinoussi , Francoise Brun-Vezinet , Christine Rouzioux , Willy Rozenbaum , Charles Dauguet , Jacqueline Gruest , Marie-Therese Nugeyre , Francoise Rey , Claudine Axler-Blin , Solange Chamaret , Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
IPC分类号： A61K35/76 , A61K35/12 , A61K35/28 , A61K39/00 , A61K39/21 , A61P31/12 , A61P31/18 , C07K14/005 , C07K14/155 , C07K14/16 , C07K14/195 , C12N5/10 , C12N7/00 , C12N7/02 , C12N15/09 , C12Q1/70 , C12R1/92 , G01N33/569 , G01N33/535 , G01N33/543
CPC分类号： C07K14/005 , C12N7/00 , G01N33/56988 , C12N2740/16021 , C12N2740/16051 , C12N2740/16222 , Y10S435/81 , Y10S435/948 , Y10S435/974 , Y10S436/804 , Y10S436/808 , Y10S436/811
摘要： Retroviruses associated with Acquired Immune Deficiency Syndrome (AIDS), including Lymphadenopathy Associated Virus (LAV), are isolated from the sera of patients afflicted with Lymphadenopathy Syndrome (LAS) or AIDS. LAV is a Human Immunodeficiency Virus (HIV). Viral extract, structural proteins and other fractions of the retrovirus immunologically recognize the sera of such patients. Immunological reaction is used to detect antibodies that specifically bind to antigenic sites of the retrovirus in samples of body fluids from patients with AIDS or risk of AIDS. A kit for in vitro assay of LAS or AIDS is provided.
摘要翻译：与获得性免疫缺陷综合征（AIDS）相关的逆转录病毒，包括淋巴结病相关病毒（LAV），从患有淋巴结病综合征（LAS）或AIDS的患者血清中分离。 LAV是人类免疫缺陷病毒（HIV）。病毒提取物，结构蛋白和其他部分的逆转录病毒免疫识别这些患者的血清。免疫反应用于检测具有艾滋病或艾滋病风险的患者体液样品中特异性结合逆转录病毒抗原位点的抗体。提供了用于LAS或AIDS的体外测定的试剂盒。

5. 发明授权

US4520113A Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions 失效
标题翻译：血清学检测艾滋病和艾滋病前期患者血清中HTLV-III的抗体
公开(公告)号：US4520113A
公开(公告)日：1985-05-28
申请号：US602945
申请日：1984-04-23
申请人： Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
发明人： Robert C. Gallo , Mikulas Popovic , Mangalasseril G. Sarngadharan
IPC分类号： G01N33/543 , A61K39/00 , C12N7/04 , C12Q1/70 , G01N33/569 , G01N33/54 , G01N33/56
CPC分类号： G01N33/56988 , Y10S435/81 , Y10S435/948 , Y10S435/974 , Y10S435/975 , Y10S436/804 , Y10S436/808 , Y10S436/809 , Y10S436/811
摘要： This invention relates to the detection of antibodies in sera of AIDS and pre-AIDS patients and describes the biochemical and immunological analysis of antigens associated with the virus HTLV-III Human T-Cell Leukemia Virus. It is shown that antigens associated with the infection of human cells by this virus are specifically recognized by antibodies from AIDS patients. Specifically, HTLV-III isolated from AIDS patients and transmitted by cocultivation with an HT cell line is specifically detected by antibodies from human sera taken from AIDS patients. The method of detection of antibodies preferred is a strip radioimmunoassay (RIA) based on the Western Blot technique or an ELISA (an enzyme-linked immunosorbent assay) or an indirect immunofluorescence assay.
摘要翻译：本发明涉及艾滋病和艾滋病前期患者血清中抗体的检测，并描述了与病毒HTLV-III人T细胞白血病病毒相关的抗原的生物化学和免疫学分析。显示出与该病毒感染人细胞相关的抗原由AIDS患者的抗体特异性地识别。具体来说，艾滋病患者分离出来的并通过HT细胞系共培养传播的HTLV-III由来自艾滋病患者的人血清的抗体特异性检测。检测抗体的方法优选是基于Western Blot技术或ELISA（酶联免疫吸附测定）或间接免疫荧光测定的条带放射免疫测定（RIA）。

6. 发明授权

US4843011A Monoclonal antibodies for binding HTLV-III proteins, and cell lines for their production 失效
标题翻译：用于结合HTLV-III蛋白的单克隆抗体，以及用于其生产的细胞系
公开(公告)号：US4843011A
公开(公告)日：1989-06-27
申请号：US816573
申请日：1986-01-03
申请人： Mangalasseril G. Sarngadharan , Fulvia di Marzo Veronese , Robert C. Gallo
发明人： Mangalasseril G. Sarngadharan , Fulvia di Marzo Veronese , Robert C. Gallo
IPC分类号： C07K16/10
CPC分类号： C07K16/1063 , C07K16/1054 , Y10S435/81 , Y10S436/808 , Y10S436/809 , Y10S436/811 , Y10S530/808 , Y10S530/809 , Y10S530/864 , Y10S930/221
摘要： Monoclonal antibodies, and hybridoma cell lines for their production, that bind with a high degree of specificity proteins associated with HTLV-III virus are presently disclosed. In particular, transmembrane envelope glycoprotein gp41 (41,000 dalton molecular size), major core antigen p24 (24,000 dalton molecular size), and p17 protein (17,000 dalton molecular size) are disclosed. The proteins to which the present monoclonal antibodies respond are essentially antigenically distinct from HTLV-I and HTLV-II. SVM-16 is an IgM monoclonal antibody, SVM-23 is an IgG.sub.2 monoclonal antibody, and SVM-26 is an IgG.sub.1 monoclonal antibody, all of which bind to p24. SVM-25 is an IgG.sub.1 monoclonal antibody binding gp41, and SVM-33 is an IgG.sub.1 monoclonal antibody binding p17. All the monoclonal antibodies of the present invention are produced in hybridoma cells prepared by fusing myeloma cells with spleen cells from mammals, such as mice, immunized with lysates of purified virus.
摘要翻译：目前公开了与HTLV-III病毒相关的高度特异性蛋白质结合的用于其生产的单克隆抗体和杂交瘤细胞系。具体地，公开了跨膜包膜糖蛋白gp41（41,000道尔顿分子大小），主要核心抗原p24（24,000道尔顿分子大小）和p17蛋白（17,000道尔顿分子大小）。本单克隆抗体应答的蛋白质本质上与HTLV-1和HTLV-II具有抗原性。 SVM-16是IgM单克隆抗体，SVM-23是IgG2单克隆抗体，SVM-26是IgG1单克隆抗体，均与p24结合。 SVM-25是结合gp41的IgG1单克隆抗体，SVM-33是结合p17的IgG1单克隆抗体。将本发明的所有单克隆抗体产生于通过将骨髓瘤细胞与来自哺乳动物（例如用纯化病毒的裂解物免疫的）的小鼠的脾细胞融合而制备的杂交瘤细胞中。

7. 发明授权

US6030772A Neutralizing antibodies against HIV infection 失效
标题翻译：中和抗HIV感染的抗体
公开(公告)号：US6030772A
公开(公告)日：2000-02-29
申请号：US75544
申请日：1998-05-11
申请人： Anthony L. Devico , Ranajit Pal , Mangalasseril G. Sarngadharan
发明人： Anthony L. Devico , Ranajit Pal , Mangalasseril G. Sarngadharan
IPC分类号： G01N33/569 , A01N65/00 , A61K39/00 , A61K39/21 , A61K39/385 , A61K39/395 , A61P31/12 , A61P31/18 , C07K14/16 , C07K14/42 , C07K14/73 , C07K16/00 , C07K16/10 , C07K16/28 , C07K19/00 , C12N5/06 , C12N5/10 , C12N5/20 , C12N15/02 , C12P21/08 , C12Q1/70 , G01N33/53 , G01N33/577
CPC分类号： C07K14/005 , C07K14/70514 , C07K16/1063 , C07K16/2812 , A61K39/00 , C12N2740/16122 , Y10S435/974 , Y10S435/975
摘要： Neutralizing antibodies against HIV infection are provided. The antibodies are reactive with cryptic epitopes on gp120 and/or CD4 induced by the formation of immunogenic complexes comprising gp120 covalently bonded to CD4 or to succinyl concanvalin A.
摘要翻译：提供抗HIV感染的中和抗体。抗体与gp120和/或CD4上的隐性表位有反应，通过形成免疫原性复合物诱导，其包含与CD4或琥珀酰胆碱素A共价结合的gp120。

8. 发明授权

US5518723A HIV immunogenic complexes 失效
标题翻译： HIV免疫复合物
公开(公告)号：US5518723A
公开(公告)日：1996-05-21
申请号：US437250
申请日：1995-05-08
申请人： Anthony L. DeVico , Ranajit Pal , Mangalasseril G. Sarngadharan
发明人： Anthony L. DeVico , Ranajit Pal , Mangalasseril G. Sarngadharan
IPC分类号： G01N33/569 , A01N65/00 , A61K39/00 , A61K39/21 , A61K39/385 , A61K39/395 , A61P31/12 , A61P31/18 , C07K14/16 , C07K14/42 , C07K14/73 , C07K16/00 , C07K16/10 , C07K16/28 , C07K19/00 , C12N5/06 , C12N5/10 , C12N5/20 , C12N15/02 , C12P21/08 , C12Q1/70 , G01N33/53 , G01N33/577
CPC分类号： C07K14/005 , C07K14/70514 , C07K16/1063 , C07K16/2812 , A61K39/00 , C12N2740/16122 , Y10S435/974 , Y10S435/975
摘要： A vaccine and a method of raising neutralizing antibodies against HIV infection. The vaccine comprises a complex of gp120 bonded covalently to CD4 or to succinyl concanavalin A.
摘要翻译：疫苗和提高抗HIV感染抗体的方法。该疫苗包含与CD4共价键合或与琥珀酰刀豆素A共价键合的gp120复合体。

9. 发明授权

US5876732A Human T-cell line infected with HIV-2 which secretes functionally intact HIV-2 GP160 失效
标题翻译：感染HIV-2的人类T细胞系分泌功能完整的HIV-2 GP160
公开(公告)号：US5876732A
公开(公告)日：1999-03-02
申请号：US59804
申请日：1998-04-14
申请人： Mangalasseril G. Sarngadharan , Vaniambadi S. Kalyanaraman , Irene LaRue-Kalisz
发明人： Mangalasseril G. Sarngadharan , Vaniambadi S. Kalyanaraman , Irene LaRue-Kalisz
IPC分类号： G01N33/569 , A61K39/00 , A61K39/21 , A61P31/12 , A61P31/18 , C07K14/155 , C07K14/16 , C07K16/10 , C12N5/06 , C12N7/00 , C12P21/02 , C12R1/92 , G01N33/53
CPC分类号： C07K14/005 , A61K39/00 , C12N2740/16122
摘要： A novel cell line chronically infected with HIV-2.sub.NIHZ virus secretes the precursor of the viral envelope proteins in extracellular medium. The cells grow well in serum-free medium which enables the concentration of the conditioned medium of the cells to be greatly increased (>100 fold). Such a concentration step is otherwise impossible to achieve if the cells are grown under conventional conditions using 10% fetal calf serum. A specific affinity procedure is provided to purify the gp160 protein from the concentrated conditioned medium of cultures of 100 liter batches using a mouse monoclonal antibody to the HIV-2 gp41 protein, which is a part of the gp160 protein. This purified HIV-2 gp160 reacts with high specificity and sensitivity with the anti viral antibodies of HIV-2 infected humans by several criteria such as i) ELISA, ii) western blot analysis, and iii) radioimmuno-precipitation of metabolically labeled gp160 from the same cell line. The purified HIV-2 gp160 from this cell line is therefore a diagnostic tool for the detection of HIV-2 infection in humans. In view of the predicted oligomeric property of gp160 and its close conformational similarity to the virion envelope, the purified gp160 can also be useful for the development of vaccine strategies against HIV-2 in humans.
摘要翻译：长期感染HIV-2NIHZ病毒的新型细胞系分泌细胞外介质中病毒包膜蛋白的前体。细胞在无血清培养基中生长良好，使得细胞条件培养基的浓度大大增加（> 100倍）。如果细胞在常规条件下使用10％胎牛血清生长，则这样的浓缩步骤是不可能实现的。提供特异性亲和性程序以使用作为gp160蛋白的一部分的HIV-2 gp41蛋白的小鼠单克隆抗体从100升批次的培养物的浓缩条件培养基中纯化gp160蛋白。这种纯化的HIV-2 gp160通过几个标准如HIV-2感染的人的抗病毒抗体具有高特异性和敏感性，例如i）ELISA，ii）蛋白质印迹分析，和iii）代谢标记的gp160的放射免疫沉淀相同的细胞系。因此，来自该细胞系的纯化的HIV-2 gp160是用于检测人类中HIV-2感染的诊断工具。鉴于gp160的预测寡聚特性及其与病毒粒子包膜的紧密构象相似性，纯化的gp160也可用于开发人类针对HIV-2的疫苗策略。

10. 发明授权

US5766844A Human T-cell line infected with HIV-2 which secretes functionally intact HIV-2 GP160. 失效
标题翻译：感染HIV-2的人类T细胞系分泌功能完整的HIV-2 GP160。
公开(公告)号：US5766844A
公开(公告)日：1998-06-16
申请号：US676865
申请日：1996-07-03
申请人： Mangalasseril G. Sarngadharan , Vaniambadi S. Kalyanaraman , Irene LaRue-Kalisz
发明人： Mangalasseril G. Sarngadharan , Vaniambadi S. Kalyanaraman , Irene LaRue-Kalisz
IPC分类号： G01N33/569 , A61K39/00 , A61K39/21 , A61P31/12 , A61P31/18 , C07K14/155 , C07K14/16 , C07K16/10 , C12N5/06 , C12N7/00 , C12P21/02 , C12R1/92 , C12Q1/70 , C07K1/00
CPC分类号： C07K14/005 , A61K39/00 , C12N2740/16122
摘要： A novel cell line chronically infected with HIV-2.sub.NIHZ virus secretes the precursor of the viral envelope proteins in extracellular medium. The cells grow well in serum-free medium which enables the concentration of the conditioned medium of the cells to be greatly increased (>100 fold). Such a concentration step is otherwise impossible to achieve if the cells are grown under conventional conditions using 10% fetal calf serum. A specific affinity procedure is provided to purify the gp160 protein from the concentrated conditioned medium of cultures of 100 liter batches using a mouse monoclonal antibody to the HIV-2 gp41 protein, which is a part of the gp160 protein. This purified HIV-2 gp160 reacts with high specificity and sensitivity with the anti viral antibodies of HIV-2 infected humans by several criteria such as i) ELISA, ii) western blot analysis, and iii) radioimmuno-precipitation of metabolically labeled gp160 from the same cell line. The purified HIV-2 gp160 from this cell line is therefore a diagnostic tool for the detection of HIV-2 infection in humans. In view of the predicted oligomeric property of gp160 and its close conformational similarity to the virion envelope, the purified gp160 can also be useful for the development of vaccine strategies against HIV-2 in humans.
摘要翻译：长期感染HIV-2NIHZ病毒的新型细胞系分泌细胞外介质中病毒包膜蛋白的前体。细胞在无血清培养基中生长良好，使得细胞条件培养基的浓度大大增加（> 100倍）。如果细胞在常规条件下使用10％胎牛血清生长，则这样的浓缩步骤是不可能实现的。提供特异性亲和性程序以使用作为gp160蛋白的一部分的HIV-2 gp41蛋白的小鼠单克隆抗体从100升批次的培养物的浓缩条件培养基中纯化gp160蛋白。这种纯化的HIV-2 gp160通过几个标准如HIV-2感染的人的抗病毒抗体具有高特异性和敏感性，例如i）ELISA，ii）蛋白质印迹分析，和iii）代谢标记的gp160的放射免疫沉淀相同的细胞系。因此，来自该细胞系的纯化的HIV-2 gp160是用于检测人类中HIV-2感染的诊断工具。鉴于gp160的预测寡聚特性及其与病毒粒子包膜的紧密构象相似性，纯化的gp160也可用于开发人类针对HIV-2的疫苗策略。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式