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1. 发明申请

US20100233696A1 METHODS, FLOW CELLS AND SYSTEMS FOR SINGLE CELL ANALYSIS 审中-公开
标题翻译：方法，流式细胞和单细胞分析系统
公开(公告)号：US20100233696A1
公开(公告)日：2010-09-16
申请号：US12612520
申请日：2009-11-04
申请人： Richard Joseph , James J. DiMeo , Mirna Jarosz , John F. Thompson , Jayson Bowers , Scott Chouinard , Philipp Kapronov , J. William Efcavitch , Christopher Hart , Fatih Ozsolak
发明人： Richard Joseph , James J. DiMeo , Mirna Jarosz , John F. Thompson , Jayson Bowers , Scott Chouinard , Philipp Kapronov , J. William Efcavitch , Christopher Hart , Fatih Ozsolak
IPC分类号： C12Q1/68 , B01L3/00 , G01N33/53
CPC分类号： B01L3/502715 , B01L3/5025 , B01L2200/028 , B01L2300/0877 , B01L2300/088 , B01L2300/123 , B01L2300/161 , B01L2400/0415 , B01L2400/0445 , B01L2400/0487 , C12Q1/6869 , Y10T436/143333
摘要： A method, flow cell and/or device for increasing the recovery of a limiting analyte in a sample, e.g., for single molecule analysis is disclosed. Methods for preparing a nucleic acid sample from a single cell and capturing nucleic acids on a surface configured for use in or with single molecule analysis are also provided.
摘要翻译：公开了用于增加样品中限制性分析物的回收的方法，流动池和/或装置，例如用于单分子分析。还提供了从单个细胞制备核酸样品并捕获配置用于单分子分析或用于单分子分析的表面上的核酸的方法。

2. 发明申请

US20120028822A1 METHODS, FLOW CELLS AND SYSTEMS FOR SINGLE CELL ANALYSIS 审中-公开
标题翻译：方法，流式细胞和单细胞分析系统
公开(公告)号：US20120028822A1
公开(公告)日：2012-02-02
申请号：US13125932
申请日：2009-11-04
申请人： Richard Joseph , James Dimeo , Mirna Jarosz , John F. Thompson , Jayson Bowers , Scott Chouinard , Philipp Kapranov , William J. Efcavitch , Christopher Hart , Fatih Ozsolak
发明人： Richard Joseph , James Dimeo , Mirna Jarosz , John F. Thompson , Jayson Bowers , Scott Chouinard , Philipp Kapranov , William J. Efcavitch , Christopher Hart , Fatih Ozsolak
IPC分类号： C40B30/04 , C40B30/00 , C12M1/34 , C12Q1/68
CPC分类号： B01L3/502715 , B01L3/5025 , B01L2200/028 , B01L2300/0877 , B01L2300/088 , B01L2300/123 , B01L2300/161 , B01L2400/0415 , B01L2400/0445 , B01L2400/0487 , C12Q1/6869 , Y10T436/143333
摘要： A method, flow cell and/or device for increasing the recovery of a limiting analyte in a sample, e.g., for single molecule analysis is disclosed. Methods for preparing a nucleic acid sample from a single cell and capturing nucleic acids on a surface configured for use in or with single molecule analysis are also provided.
摘要翻译：公开了用于增加样品中限制性分析物的回收的方法，流动池和/或装置，例如用于单分子分析。还提供了从单个细胞制备核酸样品并捕获配置用于单分子分析或用于单分子分析的表面上的核酸的方法。

3. 发明申请

US20100233697A1 METHOD FOR STANDARIZING SURFACE BINDING OF A NUCLEIC ACID SAMPLE FOR SEQUENCING ANALYSIS 审中-公开
标题翻译：标准化用于测序分析的核酸样品的表面结合方法
公开(公告)号：US20100233697A1
公开(公告)日：2010-09-16
申请号：US12618251
申请日：2009-11-13
申请人： Richard Joseph , James J. DiMeo
发明人： Richard Joseph , James J. DiMeo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2563/131
摘要： Methods are described which enable nucleic acid sample standardization prior to anchoring to a surface, especially useful in single molecule nucleic acid sequencing applications when sample is limiting or unamplified.
摘要翻译：描述了使锚定到表面之前的核酸样品标准化的方法，特别是在单分子核酸测序应用中是有用的，当样品是限制性或非扩增性的。

4. 外观设计

USD519489S1 Paging device 有权
标题翻译：寻呼设备
公开(公告)号：USD519489S1
公开(公告)日：2006-04-25
申请号：US29226267
申请日：2005-03-26
申请人： Richard Joseph
设计人： Richard Joseph

5. 发明申请

US20050208495A1 Real-time detection of nucleic acid reactions 审中-公开
标题翻译：实时检测核酸反应
公开(公告)号：US20050208495A1
公开(公告)日：2005-09-22
申请号：US10528288
申请日：2003-09-17
申请人： Richard Joseph , James DiMeo
发明人： Richard Joseph , James DiMeo
IPC分类号： G01N21/64 , C12N15/09 , C12Q1/68 , G01N20060101 , G01N21/78
CPC分类号： C12Q1/6816 , C12Q1/6818 , C12Q1/686 , C12Q2563/137 , C12Q2563/107 , C12Q2561/113
摘要： A process is provided for using oligonucleotide to which a detectable moiety is attached post-synthesis. A metal-containing fluorescent compound affords real-time detection of nucleic acid elongation, amplification, or hybridization. The process is especially advantageous since a detectable moiety is readily attached to an existing oligonucleotide at an internal nucleotide, rather than being limited to attachment at a 3′ or 5′ terminus.
摘要翻译：提供了使用合成后可检测部分连接的寡核苷酸的方法。含金属荧光化合物提供核酸延伸，扩增或杂交的实时检测。该方法是特别有利的，因为可检测的部分容易地连接到内部核苷酸上的现有寡核苷酸，而不是限于在3'或5'末端附着。

6. 发明申请

US20060166215A1 Assay and process for labeling and detection of micro rna and small interfering rna sequences 审中-公开
标题翻译：微量rna和小干扰rna序列的标记和检测的测定和方法
公开(公告)号：US20060166215A1
公开(公告)日：2006-07-27
申请号：US10563347
申请日：2004-07-02
申请人： Richard Joseph , James DiMeo
发明人： Richard Joseph , James DiMeo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6837 , C12Q2565/519 , C12Q2563/137 , C12Q2525/207
摘要： A process for protecting a short RNA fragment includes labeling a short RNA fragment with a detectable platinum compound forming a labeled small RNA fragment. Resulting labeled short RNA fragment is exposed to a capture oligonucleotide. The capture oligonucleotide includes at least two replicates of a nucleotide sequence complimentary to the short RNA fragment nucleotide sequence. The labeled short RNA fragment and the captured oligonucleotide sequence are brought into contact under hybridization conditions. With hybridization, the marker moiety is detected on the hybridized labeled small RNA fragment-captured oligonucleotide conjugant. A detection array for short RNA fragment includes a substrate having multiple spots, with a first spot including a first capture oligonucleotide including at least two replicates of a nucleotide sequence complimentary to a first short RNA fragment along with an additional nucleotide sequence functioning as a universal controller or spacer. Another spot on the array includes a second capture oligonucleotide having at least two replicates of a sequence complimentary to a second short RNA fragment along with an additional sequence functioning as a universal controller or spacer. Verified small RNA fragments are also obtained after mobilizing a sequence through elution and optional platinum compound removal therefrom.
摘要翻译：用于保护短RNA片段的方法包括用形成标记的小RNA片段的可检测铂化合物标记短RNA片段。得到的标记的短RNA片段暴露于捕获寡核苷酸。捕获寡核苷酸包括与短RNA片段核苷酸序列互补的核苷酸序列的至少两个重复。标记的短RNA片段和捕获的寡核苷酸序列在杂交条件下接触。通过杂交，在杂交标记的小RNA片段捕获的寡核苷酸共轭体上检测标记部分。用于短RNA片段的检测阵列包括具有多个点的底物，第一个斑点包含第一个捕获寡核苷酸，其包含与第一个短RNA片段互补的核苷酸序列的至少两个重复以及用作通用控制器的另外的核苷酸序列或间隔物。该阵列上的另一个点包括具有与第二短RNA片段互补的序列的至少两个重复的第二捕获寡核苷酸以及用作通用控制器或间隔物的附加序列。在通过洗脱动员序列和从其中除去任选的铂化合物后，也可以获得验证的小RNA片段。

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