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    • 5. 发明申请
    • PROBES FOR DETECTING MUTATIONS OF kRas GENE, LIQUICHIP AND DETECTION METHODS THEREOF
    • 检测kRas基因突变的检测方法及其检测方法
    • US20110269640A1
    • 2011-11-03
    • US13125196
    • 2009-10-19
    • Jiasen XuShiyang WuLifen RenJiaying HeHuiyi YangYiqun Lin
    • Jiasen XuShiyang WuLifen RenJiaying HeHuiyi YangYiqun Lin
    • C40B30/04C40B40/06C07H21/04
    • C12Q1/6886C12Q2600/156
    • Nucleic acid probes for detecting kRas gene mutations, liquid chips and detection methods thereof are provided. The liquid chips for detecting kRas gene mutations comprise: microspheres coupled with wild-type and mutant-type probes, each of which is amino-substituted at 5′-terminal, specific for kRas codons 12, 13 and/or 61, and primers for amplifying the target sequence biotin-labeled at the terminal which are enriched with mutant alleles of kRas codons 12, 13 and/or 61 to be detected. The detection methods are rapid and accurate, and the processes of the methods are easy to perform. The liquid chip can be used to detect mutations of kRas as assistance to early diagnosis of pancreatic cancer, and can be used to prognose efficiency of the molecular targeted therapy to choose right medicine accurately clinically and avoiding economic loss and time loss caused by unnecessary treatment.
    • 提供了用于检测kRas基因突变的核酸探针,液芯片及其检测方法。 用于检测kRas基因突变的液体芯片包括:与野生型和突变型探针偶联的微球体,其每个在5'端被氨基取代,对kRas密码子12,13和/或61是特异性的,以及用于 扩增在末端有生物素标记的靶序列,其富含待检测的kRas密码子12,13和/或61的突变型等位基因。 检测方法快速准确,方法简单易行。 液体芯片可用于检测kRas的突变,作为对胰腺癌早期诊断的辅助,可用于预测分子靶向治疗的效率,临床选择正确的药物,避免由不必要的治疗引起的经济损失和时间损失。