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1. 发明申请

US20120100534A1 EFFICIENT BASE DETERMINATION IN SEQUENCING REACTIONS 有权
标题翻译：顺序反应中有效的碱基测定
公开(公告)号：US20120100534A1
公开(公告)日：2012-04-26
申请号：US12329365
申请日：2008-12-05
申请人： Radoje Drmanac , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
发明人： Radoje Drmanac , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6855 , C12Q2531/125 , C12Q2521/313 , C12Q2521/125 , C12Q2537/163 , C12Q2525/191 , C12Q2521/531 , C12Q2521/501 , C12Q2525/307 , C12Q2525/151
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译：本发明涉及用于核酸鉴定和检测的组合物和方法。本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。

2. 发明申请

US20090176234A1 Efficient base determination in sequencing reactions 有权
标题翻译：测序反应中有效的碱基测定
公开(公告)号：US20090176234A1
公开(公告)日：2009-07-09
申请号：US12335188
申请日：2008-12-15
申请人： Radoje Drmanac , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
发明人： Radoje Drmanac , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6855 , C12Q2531/125 , C12Q2521/313 , C12Q2521/125 , C12Q2537/163 , C12Q2525/191 , C12Q2521/531 , C12Q2521/501 , C12Q2525/307 , C12Q2525/151
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译：本发明涉及用于核酸鉴定和检测的组合物和方法。本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。

3. 发明申请

US20100105052A1 Nucleic acid sequencing and process 有权
标题翻译：核酸测序和过程
公开(公告)号：US20100105052A1
公开(公告)日：2010-04-29
申请号：US12573697
申请日：2009-10-05
申请人： Radoje DRMANAC , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
发明人： Radoje DRMANAC , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2533/107 , C12Q2525/185 , C12Q2525/151
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译：本发明涉及用于核酸鉴定和检测的组合物和方法。本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。

4. 发明申请

US20090318304A1 Efficient Shotgun Sequencing Methods 有权
标题翻译：高效霰弹枪测序方法
公开(公告)号：US20090318304A1
公开(公告)日：2009-12-24
申请号：US12325922
申请日：2008-12-01
申请人： Radoje Drmanac , Clifford Reid
发明人： Radoje Drmanac , Clifford Reid
IPC分类号： C40B30/04
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2549/119 , C12Q2537/149 , C12Q2565/137
摘要： Methods are provided for efficient shotgun sequencing to allow efficient selection and sequencing of nucleic acids of interest contained in a library. The nucleic acids of interest can be defined any time before or after preparation of the library. One example of nucleic acids of interest is missing or low confidence genome sequences resulting from an initial sequencing procedure. Other nucleic acids of interest include subsets of genomic DNA, RNA or cDNAs (exons, genes, gene sets, transciptomes). By designing an efficient (simple to implement, speedy, high specificity, low cost) selection procedure, a more complete sequence is achieved with less effort than by using highly redundant shotgun sequencing in an initial sequencing procedure
摘要翻译：提供了有效的霰弹枪测序的方法，以允许文库中包含的感兴趣的核酸的有效选择和测序。感兴趣的核酸可以在文库制备之前或之后的任何时间进行定义。感兴趣的核酸的一个实例是由初始测序程序产生的缺失或低置信基因组序列。感兴趣的其他核酸包括基因组DNA，RNA或cDNAs（外显子，基因，基因组，转录本）的子集。通过设计一种高效（简单实施，快速，高特异性，低成本）选择程序，通过在初始测序程序中使用高度冗余的霰弹枪测序，以更少的努力实现更完整的顺序

5. 发明授权

US08298768B2 Efficient shotgun sequencing methods 有权
标题翻译：高效霰弹枪测序方法
公开(公告)号：US08298768B2
公开(公告)日：2012-10-30
申请号：US12325922
申请日：2008-12-01
申请人： Radoje Drmanac , Clifford Reid
发明人： Radoje Drmanac , Clifford Reid
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2549/119 , C12Q2537/149 , C12Q2565/137
摘要： Methods are provided for efficient shotgun sequencing to allow efficient selection and sequencing of nucleic acids of interest contained in a library. The nucleic acids of interest can be defined any time before or after preparation of the library. One example of nucleic acids of interest is missing or low confidence genome sequences resulting from an initial sequencing procedure. Other nucleic acids of interest include subsets of genomic DNA, RNA or cDNAs (exons, genes, gene sets, transciptomes). By designing an efficient (simple to implement, speedy, high specificity, low cost) selection procedure, a more complete sequence is achieved with less effort than by using highly redundant shotgun sequencing in an initial sequencing procedure.
摘要翻译：提供了有效的霰弹枪测序的方法，以允许文库中包含的感兴趣的核酸的有效选择和测序。感兴趣的核酸可以在文库制备之前或之后的任何时间进行定义。感兴趣的核酸的一个实例是由初始测序程序产生的缺失或低置信基因组序列。感兴趣的其他核酸包括基因组DNA，RNA或cDNAs（外显子，基因，基因组，转录本）的子集。通过设计一种高效（简单易于实施，快速，高特异性，低成本）选择程序，通过在初始测序程序中使用高度冗余的霰弹枪测序，以更少的努力实现更完整的顺序。

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