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1. 发明公开

EP1651760A4 HUMAN BLOOD PROTEINS EXPRESSED IN MONOCOT SEEDS 审中-公开
标题翻译：单核苷酸合成人血液中表达的蛋白质
公开(公告)号：EP1651760A4
公开(公告)日：2006-10-18
申请号：EP03714394
申请日：2003-04-11
申请人： VENTRIA BIOSCIENCE , HUANG NING , RODRIQUEZ RAYMOND L , HAGIE FRANK E , STALKER DAVID M
发明人： HUANG NING , RODRIQUEZ RAYMOND L , HAGIE FRANK E , STALKER DAVID M
IPC分类号： C12N15/00 , A01H5/00 , C07K14/75 , C07K14/81 , C12N15/63 , C12N15/82 , C12P21/00
CPC分类号： C07K14/75 , C07K14/8125 , C12N15/8257 , C12P21/005

2. 发明专利

AU2003218396A1 HUMAN BLOOD PROTEINS EXPRESSED IN MONOCOT SEEDS 未知
公开(公告)号：AU2003218396A1
公开(公告)日：2004-11-26
申请号：AU2003218396
申请日：2003-04-11
申请人： RODRIQUEZ RAYMOND L , HAGIE FRANK E , STALKER DAVID M , VENTRIA BIOSCIENCE , HUANG NING
发明人： HAGIE FRANK E , STALKER DAVID M , HUANG NING , RODRIQUEZ RAYMOND L
IPC分类号： C07K14/75 , C07K14/81 , C12N15/82 , C12P21/00 , C12N15/00 , A01H5/00 , C12N15/63

3. 发明申请

WO0003012A2 EXPRESSION OF EUKARYOTIC PEPTIDES IN PLANT PLASTIDS 审中-公开
标题翻译：真核生物肽在植物体内的表达
公开(公告)号：WO0003012A2
公开(公告)日：2000-01-20
申请号：PCT/US9915473
申请日：1999-07-10
申请人： CALGENE LLC , MCBRIDE KEVIN E , NEHRA NARENDER , RUSSELL DOUGLAS A , STALKER DAVID M
发明人： MCBRIDE KEVIN E , NEHRA NARENDER , RUSSELL DOUGLAS A , STALKER DAVID M
IPC分类号： A01H5/00 , C07K14/61 , C07K14/81 , C12N5/10 , C12N15/09 , C12N15/18 , C12N15/82 , C12P21/02 , C12P21/08 , C12N15/00
CPC分类号： C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257 , C12N15/8275
摘要： Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.
摘要翻译：提供了用于在植物质体中表达来源于真核生物的肽的构建体和方法。构建体具有在植物质体中有功能的启动子，编码来源于真核生物的肽和转录终止区的DNA序列。其他元件包括用于选择植物细胞的选择标记，所述植物细胞包含质体，所述质体表达与质体基因组同源性的标记和DNA区域，以及任选地与启动子连接的核糖体结合位点。通过使用这种构建体的方法，通过在表达DNA编码序列以在所述质体中产生真核肽的条件下生长植物细胞，在植物细胞中产生高水平的真核生物肽，例如哺乳动物蛋白质。

4. 发明申请

WO0005381A2 EXPRESSION OF ENZYMES INVOLVED IN CELLULOSE MODIFICATION 审中-公开
标题翻译：参与纤维素修饰的酶的表达
公开(公告)号：WO0005381A2
公开(公告)日：2000-02-03
申请号：PCT/US9916579
申请日：1999-07-23
申请人： CALGENE LLC , STALKER DAVID M , HIMMEL MICHAEL E , SCHAAF DAVID J , THOMAS STEVEN R
发明人： STALKER DAVID M , HIMMEL MICHAEL E , SCHAAF DAVID J , THOMAS STEVEN R
IPC分类号： A01H5/00 , C12N5/10 , C12N9/24 , C12N15/09 , C12N15/53 , C12N15/82 , A01H5/10 , C12N15/55
CPC分类号： C12N15/8246 , C12N15/8214
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide expression of cellulose degrading enzymes in the plants or plant cells.
摘要翻译：用于植物细胞基因工程的新型组合物和方法，用于在植物或植物细胞中提供纤维素降解酶的表达。

5. 发明申请

WO9818949A3 Plant cellulose synthase and promoter sequences 审中-公开
标题翻译：植物纤维素合成酶和启动子序列
公开(公告)号：WO9818949A3
公开(公告)日：1998-06-11
申请号：PCT/US9719529
申请日：1997-10-29
申请人： CALGENE INC , STALKER DAVID M , PEAR JULIE R , DELMER DEBORAH P
发明人： STALKER DAVID M , PEAR JULIE R , DELMER DEBORAH P
IPC分类号： A01H5/00 , C12N5/10 , C12N9/10 , C12N15/09 , C12N15/29 , C12N15/82 , C12Q1/68 , C12N15/54
CPC分类号： C12N9/1059 , C12N15/8222 , C12N15/8233 , C12N15/8242 , C12N15/8246 , C12N15/8261 , Y02A40/146
摘要： Provided are two plant cDNA clones that are homologs of the bacterial CelA genes that encode the catalytic subunit of cellulose synthase, derived from cotton (Gossypium hirsutum). Also provided are genomic promoter regions to these encoding regions to cellulose synthase. Methods for using cellulose synthase in cotton fiber and wood quality modification are also provided.

6. 发明申请

WO0003012A9 EXPRESSION OF EUKARYOTIC PEPTIDES IN PLANT PLASTIDS 审中-公开
标题翻译：表达植物体内的真菌菌株
公开(公告)号：WO0003012A9
公开(公告)日：2000-07-20
申请号：PCT/US9915473
申请日：1999-07-10
申请人： CALGENE LLC , MCBRIDE KEVIN E , NEHRA NARENDER , RUSSELL DOUGLAS A , STALKER DAVID M , STAUB JEFFREY M
发明人： MCBRIDE KEVIN E , NEHRA NARENDER , RUSSELL DOUGLAS A , STALKER DAVID M , STAUB JEFFREY M
IPC分类号： A01H5/00 , C07K14/61 , C07K14/81 , C12N5/10 , C12N15/09 , C12N15/18 , C12N15/82 , C12P21/02 , C12P21/08 , C12N15/15 , A01H5/10 , C12N15/62
CPC分类号： C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257 , C12N15/8275
摘要： Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.
摘要翻译：提供构建体和方法用于表达在植物质体中衍生自真核生物的肽。构建体具有在植物质体中起作用的启动子，编码来自真核生物的肽的DNA序列和转录终止区。其他元件包括用于选择植物细胞的选择性标记，其包含表达标记的质体和与质体的基因组同源的DNA区和任选的与启动子连接的核糖体结合位点。通过使用这种构建的方法，通过在条件下培养植物细胞，在植物细胞中产生高水平的真核生物肽，例如哺乳动物蛋白质，由此DNA编码序列被表达以在所述质体中产生真核肽。

7. 发明申请

WO9640924A3 COTTON FIBER TRANSCRIPTIONAL FACTORS 审中-公开
标题翻译：棉纤维转录因子
公开(公告)号：WO9640924A3
公开(公告)日：1997-02-06
申请号：PCT/US9609897
申请日：1996-06-07
申请人： CALGENE INC , MCBRIDE KEVIN , STALKER DAVID M , PEAR JULIE R , PEREZ GRAU LUIS
发明人： MCBRIDE KEVIN , STALKER DAVID M , PEAR JULIE R , PEREZ-GRAU LUIS
IPC分类号： A01H1/00 , C07K14/36 , C07K14/415 , C12N5/10 , C12N9/02 , C12N9/88 , C12N15/09 , C12N15/29 , C12N15/82 , A01H5/00
CPC分类号： C12N9/0071 , C07K14/36 , C07K14/415 , C12N9/0069 , C12N9/88 , C12N15/8233 , C12N15/825 , C12N15/8261 , Y02A40/146
摘要： Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of transcription of a DNA sequence of interest during various stages of cotton fiber development. The DNA constructs comprise a cotton fiber transcriptional initiation regulatory region associated with a gene which is expressed in cotton fiber. Also provided is novel cotton having a cotton fiber which has a natural color introduced by the expression in the cotton fiber cell, using such a construct, of pigment synthesis genes. Cotton fiber cells having color produced by genetic engineering and cotton cells comprising melanin and indigo pigments are included.

8. 发明专利

CA2525493C HUMAN BLOOD PROTEINS EXPRESSED IN ORYZA SEEDS 未知
公开(公告)号：CA2525493C
公开(公告)日：2018-10-30
申请号：CA2525493
申请日：2003-04-11
申请人： VENTRIA BIOSCIENCE
发明人： HUANG NING , RODRIQUEZ RAYMOND L , HAGIE FRANK E , STALKER DAVID M
IPC分类号： C12N15/00 , C07K14/75 , C07K14/81 , C12N15/63 , C12N15/82 , C12P21/00
摘要： The present disclosure relates to a method of producing a recombinant human blood protein in Oryza plant seeds, comprising the steps of transforming an Oryza plant cell with a chimeric gene, growing an Oryza plant from the transformed Oryza plant cell for a time sufficient to produce seeds containing the human blood protein; and harvesting the seeds from the Oryza plant, wherein the human blood protein constitutes at least 3.0% of the total soluble protein in the harvested seeds. In the present disclosure, the chimeric gene comprises (i) a promoter from the gene of a maturation-specific monocot plant storage protein, wherein the promoter is operably linked to a first DNA molecule encoding a monocot plant seed-specific signal sequence capable of targeting a polypeptide linked thereto to a monocot plant seed endosperm cell, wherein the promoter operably linked to the first DNA molecule encoding the signal sequence has at least 95% sequence identity over the entire length of a sequence selected from the group consisting of SEQ ID NOs:6-21; and (ii) a second DNA molecule, linked in translation frame with the first DNA molecule, encoding the human blood protein and wherein the first DNA molecule and the second DNA molecule together encode a fusion protein comprising the monocot plant seed-specific signal sequence and the human blood protein.

9. 发明专利

AU2003218396B2 Human blood proteins expressed in monocot seeds 未知
公开(公告)号：AU2003218396B2
公开(公告)日：2008-09-04
申请号：AU2003218396
申请日：2003-04-11
申请人： VENTRIA BIOSCIENCE
发明人： STALKER DAVID M , HAGIE FRANK E , HUANG NING , RODRIQUEZ RAYMOND L
IPC分类号： C12N15/82 , C07K14/75 , C07K14/81 , C12P21/00

10. 发明专利

CA2525493A1 HUMAN BLOOD PROTEINS EXPRESSED IN MONOCOT SEEDS 未知
公开(公告)号：CA2525493A1
公开(公告)日：2004-11-18
申请号：CA2525493
申请日：2003-04-11
申请人： VENTRIA BIOSCIENCE
发明人： STALKER DAVID M , HAGIE FRANK E , HUANG NING , RODRIQUEZ RAYMOND L
IPC分类号： C12N15/00 , A01H5/00 , C07K14/75 , C07K14/81 , C12N15/63 , C12N15/82 , C12P21/00
摘要： The invention is directed to blood proteins produced in monocot seeds and isolated therefrom for use in therapeutic compositions, and to methods of making these isolated blood proteins and to therapeutic compositions comprising them.

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