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1. 发明申请

WO0227315A3 COMPOUNDS WITH A BRANCHED LINKER 审中-公开
标题翻译：具有分支链接器的化合物
公开(公告)号：WO0227315A3
公开(公告)日：2002-07-11
申请号：PCT/EP0111118
申请日：2001-09-26
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , ANDRES HERBERT , JOSEL HANS-PETER , HOESS EVA , HERRMANN RUPERT , ELTZ HERBERT VON DER
发明人： ANDRES HERBERT , JOSEL HANS-PETER , HOESS EVA , HERRMANN RUPERT , VON DER ELTZ HERBERT
IPC分类号： G01N33/543 , C07F15/00 , C07K1/107 , C07K5/02 , C07K7/02 , C07K14/00 , C07K14/16 , C07K19/00 , C12N15/09 , C12Q1/68 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/547 , G01N33/58 , G01N33/68 , C07K14/18
CPC分类号： G01N33/6845 , C07K1/1075 , C07K1/1077 , C07K14/001 , G01N33/5306 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/68
摘要： The present invention concerns new compounds comprising a branched linker and their use for producing conjugates for applications in diagnostic or therapeutic methods.
摘要翻译：本发明涉及包含支链接头的新化合物及其用于制备用于诊断或治疗方法的缀合物的用途。

2. 发明申请

WO0227317A3 CONJUGATES OF DEFINED STOICHIOMETRY 审中-公开
标题翻译：定义STOICHIOMETRY的结合
公开(公告)号：WO0227317A3
公开(公告)日：2003-09-12
申请号：PCT/EP0111101
申请日：2001-09-26
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , HOESS EVA , ANDRES HERBERT , DONIE FREDERIC , VOGEL RUDOLF , JOSEL HANS-PETER , HERRMANN RUPERT , ELTZ HERBERT VON DER
发明人： HOESS EVA , ANDRES HERBERT , DONIE FREDERIC , VOGEL RUDOLF , JOSEL HANS-PETER , HERRMANN RUPERT , VON DER ELTZ HERBERT
IPC分类号： A61K47/48 , C07K1/107 , G01N30/88 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/566 , G01N33/68
CPC分类号： C07K1/1077 , A61K47/643 , A61K47/6889 , G01N33/5306 , G01N33/531 , G01N33/68 , G01N33/6845 , Y10S435/961 , Y10S435/962
摘要： The invention relates to a process for the production of a biomolecule-linker conjugate of uniform stochiometry. It especially relates to a conjugate consisting of a biomolecule of a molecular weight between 5 kD and 500 kD and a hydrophilic linker molecule said linker having a molecular weight between 1 and 15 kD and between 4 and 60 charged residues, characterized in that said conjugate comprises at least one biomelecule-linker product of uniform stoichiometry in a pre-selected amount.
摘要翻译：本发明涉及一种制备均匀化学计量学的生物分子 - 接头共轭物的方法。其特别涉及由分子量在5kD至500kD之间的生物分子和亲水性接头分子所组成的共轭物，所述接头分子量为1至15kD，4至60个带电荷的残基，其特征在于所述共轭物包含至少一种具有预选量的均匀化学计量的生物分子 - 接头产物。

3. 发明申请

WO2007140962A3 IMPROVED MEASUREMENT OF VITAMIN D 审中-公开
标题翻译：改善维生素D的测定
公开(公告)号：WO2007140962A3
公开(公告)日：2008-01-31
申请号：PCT/EP2007004924
申请日：2007-06-04
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , KOBOLD UWE , DUELFFER THOMAS , GROL MICHAEL , HERRMANN RUPERT , ELTZ HERBERT VON DER , VON PROFF LEOPOLD
发明人： KOBOLD UWE , DUELFFER THOMAS , GROL MICHAEL , HERRMANN RUPERT , VON DER ELTZ HERBERT , VON PROFF LEOPOLD
IPC分类号： G01N33/82
CPC分类号： G01N33/82 , Y10T436/20 , Y10T436/203332
摘要： The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.
摘要翻译：本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。

4. 发明申请

WO2007140961A8 DIFFERENTIAL HEMOLYSIS OF A WHOLE BLOOD SAMPLE 审中-公开
标题翻译：全血样品的差异分析
公开(公告)号：WO2007140961A8
公开(公告)日：2008-11-06
申请号：PCT/EP2007004923
申请日：2007-06-04
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , KOBOLD UWE , DUELFFER THOMAS , HERRMANN RUPERT , ELTZ HERBERT VON DER
发明人： KOBOLD UWE , DUELFFER THOMAS , HERRMANN RUPERT , VON DER ELTZ HERBERT
IPC分类号： G01N30/06 , G01N1/38 , G01N30/02 , G01N30/60 , G01N30/72 , G01N33/50
CPC分类号： G01N30/06 , G01N30/603 , G01N30/7233 , G01N33/5002 , G01N2030/027 , Y10T436/24 , Y10T436/25
摘要： The invention relates to a method for differentially hemolyzing whole blood. It discloses method for detecting an analyte in a liquid sample known or suspected to comprise red blood cells and suspected or known to comprise eukaryotic cells, the method comprising the steps of processing said liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may comprise both erythrocytes as well as nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.
摘要翻译：本发明涉及一种使全血不同溶血的方法。它公开了用于检测已知或怀疑包含红细胞并且怀疑或已知包含真核细胞的液体样品中的分析物的方法，所述方法包括以下步骤：在适合于裂解细胞膜的条件下用膜增溶剂处理所述液体样品的红细胞，同时不会引起样品成分沉淀，对经处理的样品进行色谱分离，并检测分析物。在检测可能包含红细胞和有核细胞的液体样品中的分析物的方法中，红细胞的差异溶血是有利的。红细胞的差异溶解可以容易地与诸如LC-MS的在线检测方法组合，并且在许多分析物的检测中是有利的，例如，在检测叶酸或免疫抑制药物，如他克莫司或西罗莫司。

5. 发明申请

WO02099097A8 NOVEL COMPOUNDS FOR CHEMILUMINESCENSE PROCEDURES 审中-公开
标题翻译：新型化合物治疗慢性淋巴细胞白血病
公开(公告)号：WO02099097A8
公开(公告)日：2003-03-06
申请号：PCT/EP0205855
申请日：2002-05-28
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , HEINDL DIETER , HERRMANN RUPERT , JOSEL HANS-PETER , HUBER ERASMUS , KLAUSE URSULA
发明人： HEINDL DIETER , HERRMANN RUPERT , JOSEL HANS-PETER , HUBER ERASMUS , KLAUSE URSULA
IPC分类号： G01N21/76 , A61K39/00 , A61K48/00 , C07D219/06 , C07D277/66 , C07D401/12 , C07D413/06 , C07D417/04 , C07D417/14 , C07K16/18 , C09B15/00 , C09B57/00 , C12N5/10 , C12N9/18 , C12N15/62 , C12Q1/28 , C12Q1/66 , C12Q1/68 , G01N33/50 , G01N33/53 , G01N33/533 , G01N33/58
CPC分类号： C07D401/12 , C07D219/06 , C07D417/04 , C07D417/14 , C12Q1/28 , G01N33/533 , G01N33/582 , Y10S435/968 , Y10S436/80 , Y10S436/904
摘要： The present invention relates to a chemical compound comprising a light emitting moiety precursor (eg. acridine, luciferine) and a precursor of a leaving group (eg. oxazine, anilide), bound to each other by an amide or by an ester bond and characterized in that the leaving group precursor upon oxidation is converted into the leaving group. The invention also relates to compounds additionally comprising a coupling group to the use of such compounds for labeling of biomolecules and more generally to the use of such compounds in chemiluminescence detection procedures.
摘要翻译：本发明涉及包含发光部分前体（例如吖啶，荧光素）和离去基团（例如恶嗪，酰苯胺）的前体的化合物，其通过酰胺或酯键彼此结合并表征因为氧化后的离去基团前体被转化成离去基团。本发明还涉及另外包含使用这种化合物的偶联基团用于标记生物分子的化合物，更一般地，在化学发光检测程序中使用这些化合物。

6. 发明申请

WO9714696A3 HETEROCYCLIC DIOXETHANE SUBSTRATES, PROCESS FOR THEIR PREPARATION AND THEIR USE 审中-公开
标题翻译：杂环二氧化钛基质，其制备方法及其使用
公开(公告)号：WO9714696A3
公开(公告)日：1997-06-05
申请号：PCT/EP9604506
申请日：1996-10-17
申请人： BOEHRINGER MANNHEIM GMBH , HEINDL DIETER , JOSEL HANS PETER , ELTZ HERBERT VON DER , HERRMANN RUPERT , HOELTKE HANS JOACHIM , BECKERT RAINER , WEISS DIETER , ADAM WALDEMAR
发明人： HEINDL DIETER , JOSEL HANS-PETER , VON DER ELTZ HERBERT , HERRMANN RUPERT , HOELTKE HANS-JOACHIM , BECKERT RAINER , WEISS DIETER , ADAM WALDEMAR
IPC分类号： C07D321/00 , C07D491/113 , C07D493/10 , C07F7/18 , C07F9/38 , C07F9/6561 , C07J1/00 , C07J17/00 , C07J21/00 , C07J51/00 , G01N33/533 , G01N33/58
CPC分类号： C07D493/10 , C07D321/00 , C07F7/1856 , C07F9/6561 , C07J17/00 , C07J21/00 , C07J51/00 , G01N33/582
摘要： PCT No. PCT/EP96/04506 Sec. 371 Date Apr. 20, 1998 Sec. 102(e) Date Apr. 20, 1998 PCT Filed Oct. 17, 1996 PCT Pub. No. WO97/14696 PCT Pub. Date Apr. 24, 1997Compounds of the general formula Ia and Ib are described in which R1 and R2 are the same or different and represent hydrogen, straight-chain or branched C1 to C6 lower alkyl or an aryl group optionally substituted by electron-withdrawing groups, R3 denotes a cleavable group, W is hydrogen, halogen or a pseudohalogen and at least one of the groups R4 or R5 is a group stabilizing the dioxetane structure and at most one of the groups R4 or R5 represents hydrogen and X or Y represents oxygen, N-R or C(R)2 in which R has the meanings stated for R1 and R2 or represents a mesomeric double bond or a carbonyl group and n denotes the number 0 or 1 and m denotes the number 1 or 2, as well as a process for their production. These compounds are new and can be used as substrates in immunological assays and in DNA diagnostics using activating agents for colour formation.

7. 发明申请

WO9933867A3 MODIFIED NUCLEIC ACID ANALOGUES 审中-公开
标题翻译：修饰的核酸类似物
公开(公告)号：WO9933867A3
公开(公告)日：1999-09-02
申请号：PCT/EP9808317
申请日：1998-12-18
申请人： ROCHE DIAGNOSTICS GMBH , BERGMANN FRANK , ELTZ HERBERT VON DER , HERRMANN RUPERT , KOEHLER STEFANIE , SCHLUETER VOLKER
发明人： BERGMANN FRANK , VON DER ELTZ HERBERT , HERRMANN RUPERT , KOEHLER STEFANIE , SCHLUETER VOLKER
IPC分类号： C07H21/00 , C07K14/00 , C12Q1/68
CPC分类号： C07K14/003 , C07H21/00 , C12Q1/6832 , Y02P20/55 , C12Q2563/173 , C12Q2527/119 , C12Q2525/113
摘要： Oligomers comprising at least one non-nucleoside unit containing an acid function with a pKa that is lower than 3.0 are particularly suitable as detection probes for nucleic acids as non-specific interactions are reduced in comparison with unmodified oligomers. Even when the concentration of hybridisation buffers is low, it is possible to obtain high signal dynamics and high sensitivity for a low blank signal. Said oligomers are also suitable as primers for lengthening reactions using polymerases and for transfection.
摘要翻译：具有包含具有小于3.0的pKa的酸功能的至少一个非核苷单元的低聚物，特别适合作为检测探针对核酸，因为非特异性相互作用比较了与未修饰的低聚物减小，特别是在杂交的低盐浓度缓冲液在高信号的动态和灵敏度可以在低空白信号下实现。另外，它们也适合用作使用聚合酶进行延伸反应和用于转染的引物。

8. 发明专利

ES2417140T3 Preparación de muestras para CL-EM/EM mediante la utilización de partículas magnéticas 未知
公开(公告)号：ES2417140T3
公开(公告)日：2013-08-06
申请号：ES08010569
申请日：2008-06-11
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE
发明人： KOBOLD UWE , GEIGER ALBERT , HERRMANN RUPERT , VOGESER MICHAEL
IPC分类号： B01D15/32 , B01J20/28 , B01J20/287 , B01J20/32 , G01N33/543 , G01N33/68
摘要： Un método para purificar un compuesto a partir de una muestra biológica líquida compleja, dicho compuesto se selecciona a partir del grupo que incluye itraconazol, sirólimus, tacrólimus, everólimus y ácido micofenólico, y dicho método incluye los pasos de (a) poner en contacto la muestra con una cantidad de partículas magnéticas funcionalizadas con una superficie hidrofóbica que se funcionaliza con un grupo alquilo C18, y el compuesto es capaz de interactuar directamente con la superficie hidrofóbica, o capaz de participar en interacciones hidrofóbicas tras la adición de reactivos de apareamiento iónico, y en el que la proporción partículas/muestra es igual o mayor a 1, 25 mg/ml, (b) incubar la muestra y las partículas, adsorbiendo así el compuesto en la superficie hidrofóbica, (c) separar las partículas mediante la aplicación de campos magnéticos y sustraer el líquido, (d) lavar las partículas de manera opcional, (e) eluir el compuesto a partir de las partículas, caracterizado de manera que en el paso (e) el itraconazol eluye con una mezcla seleccionada a partir del grupo que incluye (i) una mezcla de 1-9 partes en volumen de acetonitrilo y 1 parte en volumen de ácido fórmico (0, 1% [v/v]), y (ii) una mezcla de 9 partes en volumen de metanol y 1 parte en volumen de ácido fórmico (0, 1% [v/v]), y en el paso (e) el sirólimus, el tacrólimus, el everólimus o el ácido micofenólico eluyen con una mezcla de 9 partes en volumen de metanol y 1 parte en volumen de agua. En una realización particular de la invención la muestra se selecciona a partir del grupo que incluye suero, plasma, sangre total, y sangre hemolizada.

9. 发明专利

DE602004023612D1 未知
公开(公告)号：DE602004023612D1
公开(公告)日：2009-11-26
申请号：DE602004023612
申请日：2004-07-07
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE
发明人： HEINDL DIETER , HERRMANN RUPERT , JENNI WOLFGANG , JOSEL HANS-PETER , LAMPERT GUNTER
IPC分类号： C07D209/60 , C07D403/12 , G01N33/533

10. 发明专利

JP2011088892A New fluorescence quencher molecule, and method for utilizing the same and use thereof 有权
标题翻译：新型荧光探针分子及其利用方法及其应用
公开(公告)号：JP2011088892A
公开(公告)日：2011-05-06
申请号：JP2010234727
申请日：2010-10-19
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： BECKERT RAINER , BERGMANN FRANK , HEINDL DIETER , HERRMANN RUPERT , JOSEL HANS-PETER DR , WELZEL THOMAS
IPC分类号： C07D401/04 , C12N15/09 , C12Q1/68 , G01N21/78
CPC分类号： C07D401/04
摘要： PROBLEM TO BE SOLVED: To provide a new quencher preferably having low background signal and/or high quenching efficiency. SOLUTION: The invention relates to: novel pyridinyl-isoquinoline-dione derivatives; methods of producing these derivatives; conjugates comprising novel pyridinyl-isoquinoline-dione derivatives and (i) a solid support, or (ii) a biomolecule; methods of producing these conjugates; and the use of these conjugates as quenchers in fluorescence resonance energy transfer (FRET). COPYRIGHT: (C)2011,JPO&INPIT

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