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1. 发明申请

WO2003000878A3 USE OF FKBP CHAPERONES AS EXPRESSION TOOL 审中-公开
公开(公告)号：WO2003000878A3
公开(公告)日：2003-01-03
申请号：PCT/EP2002/006957
申请日：2002-06-24
申请人： ROCHE DIAGNOSTICS GMBH , F.HOFFMANN-LA ROCHE AG
发明人： SCHOLZ, Christian , ANDRES, Herbert , FAATZ, Elke , ENGEL, Alfred , SCHMITT, Urban , BAZARSUREN, Ariuna , SCHAARSCHMIDT, Peter
IPC分类号： C12N15/62
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria such as Escherichia coli . In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor, methods of recombinant protein expression, the recombinant polypeptides thus obtained as well as to the use of such polypeptides.

2. 发明申请

WO2013107633A1 SOLUBLE IMMUNOREACTIVE TREPONEMA PALLIDUM TPN47 ANTIGENS 审中-公开
标题翻译：可溶性免疫反应性TREPONEMA PALLIDUM TPN47抗原
公开(公告)号：WO2013107633A1
公开(公告)日：2013-07-25
申请号：PCT/EP2013/000108
申请日：2013-01-16
申请人： ROCHE DIAGNOSTICS GMBH , F.HOFFMANN-LA ROCHE AG , FAATZ, Elke , SCHAARSCHMIDT, Peter , SCHMITT, Urban , SCHOLZ, Christian
发明人： FAATZ, Elke , SCHAARSCHMIDT, Peter , SCHMITT, Urban , SCHOLZ, Christian
IPC分类号： C07K14/20 , C12N15/62
CPC分类号： G01N33/571 , C07K14/20 , C07K2319/00 , G01N2333/20 , G01N2469/20
摘要： The invention concerns soluble variants of Treponema pallidum antigen 47 (TpN47 antigen) comprising at least domain B, or at least domains A and B, optionally domain D of the complete TpN47 protein molecule with the proviso that all antigens lack domain C (amino acid residues 224 to 351) of TpN47. The Tpn47 antigens can be fused to a chaperone. Moreover, the invention covers DNA encoding the antigens, a method of producing these antigens as well as the use of these antigens in an immunodiagnostic assay for the detection of antibodies against Treponema pallidum in an isolated sample.
摘要翻译：本发明涉及包含至少结构域B或者至少结构域A和B，完全TpN47蛋白质分子的任选区域D的梅毒螺旋体抗原47（TpN47抗原）的可溶性变体，条件是所有抗原缺失结构域C（氨基酸残基 224〜351）TpN47。 Tpn47抗原可以融合到伴侣。此外，本发明涵盖编码抗原的DNA，制备这些抗原的方法以及在免疫诊断测定中使用这些抗原来检测分离的样品中针对梅毒螺旋体的抗体。

3. 发明申请

WO2007077008A1 CHIMAERIC FUSION PROTEIN WITH SUPERIOR CHAPERONE AND FOLDING ACTIVITIES 审中-公开
标题翻译：具有超级CHAPERONE和折叠活动的CHIMAERIC融合蛋白
公开(公告)号：WO2007077008A1
公开(公告)日：2007-07-12
申请号：PCT/EP2006/012599
申请日：2006-12-29
申请人： ROCHE DIAGNOSTICS GMBH , F.HOFFMANN-LA ROCHE AG , SCHOLZ, Christian , SCHAARSCHMIDT, Peter , SCHMITT, Urban , FAATZ, Elke
发明人： SCHOLZ, Christian , SCHAARSCHMIDT, Peter , SCHMITT, Urban , FAATZ, Elke
IPC分类号： C12N9/90 , C12N15/62 , C12N15/61 , C07K19/00 , C07K14/16 , A61K38/00 , G01N33/53
CPC分类号： C12N9/90 , C07K2319/35
摘要： The invention discloses the cloning, expression and uses of a chimaeric fusion protein with superior chaperone and folding activities compared to the wild type chaperones. This invention relates to a chimaeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for an FK506 binding protein (FKBP) or an FK506-binding- protein-like domain (FKBP-like domain). In particular, this invention relates to a chimaeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for a human FKBP type peptidyl-prolyl- cis/trαns isomerase (PPIase), methods of producing these chimaeric fusion proteins and their uses as folding helpers in the production of other proteins and in the process of the production of vaccines or pharmaceuticals, and as folding helpers for performing immunoassays.
摘要翻译：本发明公开了与野生型伴侣相比，具有优异伴侣和折叠活性的嵌合融合蛋白的克隆，表达和用途。本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码FK506结合蛋白（FKBP）或FK506-结合蛋白 - 像域（FKBP样域）。特别地，本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码人FKBP型肽基 - 脯氨酰 - 顺/反异构酶的核苷酸序列（PPIase），生产这些嵌合融合蛋白的方法及其在生产其他蛋白质和疫苗或药物生产过程中作为折叠助剂的用途，以及用于进行免疫测定的折叠助剂。

4. 发明授权

EP2127679B1 SLpA AS A TOOL FOR RECOMBINANT PROTEIN AND ENZYME TECHNOLOGY 有权
标题翻译： SLPA作为一种工具，用于重组蛋白和酶技术
公开(公告)号：EP2127679B1
公开(公告)日：2016-06-29
申请号：EP09006933.7
申请日：2009-05-25
申请人： Roche Diagnostics GmbH , F.Hoffmann-La Roche AG
发明人： FAATZ, Elke , SCHAARSCHMIDT, Peter , SCHMITT, Urban , SCHOLZ, Christian
IPC分类号： C12N15/31 , C12N15/62 , C07K14/245 , C12N9/90 , G01N33/543 , G01N33/569 , G01N33/536
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： The present invention relates to a recombinant DNA molecule encoding a fusion protein comprising a SIpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Another aspect of the invention is a method for producing said fusion protein as well as a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein as a binding partner or as a means for the reduction of interferences in an immunoassay. Further the invention relates to the use of the recombinantly produced fusion protein for immunization of laboratory animals in order to produce antibodies and to the use of the recombinantly produced fusion protein in the production of a vaccine. Yet another aspect is a method for the detection of an analyte in an immunoassay using a recombinantly produced fusion protein as well as a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention concerns the use of SlpA for the reduction of interferences in an immunoassay and its use as an additive in protein formulations and as a folding helper in biotechnological applications.

5. 发明授权

EP2222694B1 NOVEL RUBELLA E1 ENVELOPE PROTEIN VARIANTS AND THEIR USE IN THE DETECTION OF ANTI-RUBELLA ANTIBODIES 有权转让
标题翻译：风疹E1包膜蛋白和新版本它们在的抗风疹抗体探测中
公开(公告)号：EP2222694B1
公开(公告)日：2014-01-15
申请号：EP08860033.3
申请日：2008-12-11
申请人： Roche Diagnostics GmbH , F.Hoffmann-La Roche AG
发明人： SCHOLZ, Christian , BOLLHAGEN, Ralf , ENGEL, Alfred , FAATZ, Elke , SCHAARSCHMIDT, Peter , UPMEIER, Barbara , ZARNT, Toralf
IPC分类号： G01N33/569 , C07K14/005
CPC分类号： G01N33/56983 , C07K14/005 , C07K2319/35 , C12N2770/36222 , G01N2333/19 , G01N2469/20

6. 发明授权

EP2804874B1 SOLUBLE IMMUNOREACTIVE TREPONEMA PALLIDUM TPN47 ANTIGENS 有权
标题翻译：可溶性免疫反应性梅毒螺旋体，苍白球，TpN47抗原
公开(公告)号：EP2804874B1
公开(公告)日：2015-12-02
申请号：EP13705906.9
申请日：2013-01-16
申请人： Roche Diagnostics GmbH , F.Hoffmann-La Roche AG
发明人： FAATZ, Elke , SHAARSCHMIDT, Peter , SCHMITT, Urban , SCHOLZ, Christian
IPC分类号： C07K14/20 , C12N15/62
CPC分类号： G01N33/571 , C07K14/20 , C07K2319/00 , G01N2333/20 , G01N2469/20

7. 发明公开

EP2804874A1 SOLUBLE IMMUNOREACTIVE TREPONEMA PALLIDUM TPN47 ANTIGENS 有权
标题翻译：可溶性免疫反应性梅毒螺旋体，苍白球，TpN47抗原
公开(公告)号：EP2804874A1
公开(公告)日：2014-11-26
申请号：EP13705906.9
申请日：2013-01-16
申请人： Roche Diagnostics GmbH , F.Hoffmann-La Roche AG
发明人： FAATZ, Elke , SHAARSCHMIDT, Peter , SCHMITT, Urban , SCHOLZ, Christian
IPC分类号： C07K14/20 , C12N15/62
CPC分类号： G01N33/571 , C07K14/20 , C07K2319/00 , G01N2333/20 , G01N2469/20
摘要： The invention concerns soluble variants of
Treponema pallidum antigen 47 (TpN47 antigen) comprising at least domain B, or at least domains A and B, optionally domain D of the complete TpN47 protein molecule with the proviso that all antigens lack domain C (amino acid residues 224 to 351) of TpN47. The Tpn47 antigens can be fused to a chaperone. Moreover, the invention covers DNA encoding the antigens, a method of producing these antigens as well as the use of these antigens in an immunodiagnostic assay for the detection of antibodies against
Treponema pallidum in an isolated sample.

8. 发明授权

EP1402041B1 USE OF FKBP CHAPERONES AS EXPRESSION TOOL 有权
标题翻译： FKBP分子伴侣作为表达工具使用
公开(公告)号：EP1402041B1
公开(公告)日：2009-10-07
申请号：EP02747428.7
申请日：2002-06-24
申请人： Roche Diagnostics GmbH , F.HOFFMANN-LA ROCHE AG
发明人： SCHOLZ, Christian , ANDRES, Herbert , FAATZ, Elke , ENGEL, Alfred , SCHMITT, Urban , BAZARSUREN, Ariuna , SCHAARSCHMIDT, Peter
IPC分类号： C12N15/62
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor, methods of recombinant protein expression, the recombinant polypeptides thus obtained as well as to the use of such polypeptides.

9. 发明公开

EP1402041A2 USE OF FKBP CHAPERONES AS EXPRESSION TOOL 有权
标题翻译： FKBP分子伴侣作为表达工具使用
公开(公告)号：EP1402041A2
公开(公告)日：2004-03-31
申请号：EP02747428.7
申请日：2002-06-24
申请人： Roche Diagnostics GmbH , F.HOFFMANN-LA ROCHE AG
发明人： SCHOLZ, Christian , ANDRES, Herbert , FAATZ, Elke , ENGEL, Alfred , SCHMITT, Urban , BAZARSUREN, Ariuna , SCHAARSCHMIDT, Peter
IPC分类号： C12N15/62
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor, methods of recombinant protein expression, the recombinant polypeptides thus obtained as well as to the use of such polypeptides.

10. 发明公开

EP2222694A2 NOVEL RUBELLA E1 ENVELOPE PROTEIN VARIANTS AND THEIR USE IN THE DETECTION OF ANTI-RUBELLA ANTIBODIES 有权转让
标题翻译：风疹E1包膜蛋白和新版本它们在的抗风疹抗体探测中
公开(公告)号：EP2222694A2
公开(公告)日：2010-09-01
申请号：EP08860033.3
申请日：2008-12-11
申请人： F.HOFFMANN-LA ROCHE AG
发明人： SCHOLZ, Christian , BOLLHAGEN, Ralf , ENGEL, Alfred , FAATZ, Elke , SCHAARSCHMIDT, Peter , UPMEIER, Barbara , ZARNT, Toralf
IPC分类号： C07K14/19 , G01N33/53
CPC分类号： G01N33/56983 , C07K14/005 , C07K2319/35 , C12N2770/36222 , G01N2333/19 , G01N2469/20
摘要： The invention relates to soluble Rubella El antigens and variants of these antigens. The antigens comprise amino acids 201 to 432 or 169 to 432 and are lacking amino acids 453 to 481 as well as at least the amino acids 143 to 164. They further contain a region spanning two disulfide-bridges. The invention also relates to a recombinant DNA molecule encoding said Rubella E1 antigens, the expression of Rubella E1 antigens as chaperone fusion proteins and their use in a method of detecting antibodies against Rubella in a sample.

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