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1. 发明申请

WO2013164477A1 A GLYCOSYLATED MODIFIED FLAVIN ADENINE DINUCLEOTIDE DEPENDENT GLUCOSE DEHYDROGENASE 审中-公开
标题翻译： GLYCOSYLATED改性FLAVIN ADENINE DINUCLEOTIDE依赖性葡萄糖脱氢酶
公开(公告)号：WO2013164477A1
公开(公告)日：2013-11-07
申请号：PCT/EP2013/059313
申请日：2013-05-03
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG
发明人： DÜFEL, Hartmut , MEIER, Thomas , TACKE, Michael
IPC分类号： C12N9/00
CPC分类号： C12Q1/54 , C12N9/0006 , C12Y101/05 , C12Y101/9901
摘要： Subject matter of the present invention is a modified flavin adenine dinucleotide dependent glucose dehydrogenase or an active fragment thereof that is glycosylated, wherein at least one of the asparagine residues selected from the group consisting of N2; N168 and N346 according to the mature Aspergillus oryzae FAD-GDH wild-type sequence SEQ ID No: 2 (Wild type) has been substituted by one or more amino acids not suitable for glycosylation and thereby eliminating or inactivating, respectively, a potential glycosylation site at this position. Said modified flavin adenine dinucleotide dependent dehydrogenase derived from mature Aspergillus oryzae FAD-GDH wild-type sequence SEQ ID No: 2 (Wild type) or functional fragments thereof have one or more of the following substitutions N2S, N168P, N168SP, and N346D.
摘要翻译：本发明的主题是修饰的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶或其糖基化的活性片段，其中至少一种选自N2的天冬酰胺残基; 根据成熟米曲霉FAD-GDH野生型序列SEQ ID No：2（野生型）的N168和N346已被一个或多个不适合糖基化的氨基酸取代，从而分别消除或失活潜在的糖基化位点在这个位置。衍生自成熟米曲霉FAD-GDH野生型序列SEQ ID No：2（野生型）或其功能片段的经修饰的黄素腺嘌呤二核苷酸依赖性脱氢酶具有以下取代中的一个或多个取代基N2S，N168P，N168SP和N346D。

2. 发明授权

EP2844747B1 A GLYCOSYLATED MODIFIED FLAVIN ADENINE DINUCLEOTIDE DEPENDENT GLUCOSE DEHYDROGENASE 有权转让
公开(公告)号：EP2844747B1
公开(公告)日：2019-03-20
申请号：EP13723713.7
申请日：2013-05-03
申请人： Roche Diabetes Care GmbH , F. Hoffmann-La Roche AG
发明人： DÜFEL, Hartmut , MEIER, Thomas , TACKE, Michael
IPC分类号： C12N9/00

3. 发明公开

EP2844747A1 A GLYCOSYLATED MODIFIED FLAVIN ADENINE DINUCLEOTIDE DEPENDENT GLUCOSE DEHYDROGENASE 有权转让
标题翻译：甘氨酸改性氟维那定 - 丁酸胆碱酯酶葡糖苷酶
公开(公告)号：EP2844747A1
公开(公告)日：2015-03-11
申请号：EP13723713.7
申请日：2013-05-03
申请人： Roche Diagnostics GmbH , F.Hoffmann-La Roche AG
发明人： DÜFEL, Hartmut , MEIER, Thomas , TACKE, Michael
IPC分类号： C12N9/00
CPC分类号： C12Q1/54 , C12N9/0006 , C12Y101/05 , C12Y101/9901
摘要： Compositions, devices, kits and methods are disclosed for assaying glucose with a glycosylated, modified flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), variant thereof or an active fragment thereof, where at least one asparagine residue at positions N2, N168 and N346 of mature, wild-type A. oryzae FAD-GDH according to SEQ ID NO:2 is substituted by one or more amino acids not suitable for glycosylation, thereby eliminating or inactivating, respectively, a potential glycosylation site at this position.
摘要翻译：公开了用糖基化的修饰的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FAD-GDH），其变体或其活性片段测定葡萄糖的组合物，装置，试剂盒和方法，其中N 2，N 16 8和N 16 8的至少一个天冬酰胺残基根据SEQ ID NO：2的成熟的野生型米曲霉FAD-GDH的N346被一个或多个不适于糖基化的氨基酸取代，从而分别消除或灭活该位置上的潜在糖基化位点。

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