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1. 发明申请

WO9951625A9 PEPTIDES CAUSING FORMATION OF COMPACT STRUCTURES 审中-公开
标题翻译：导致紧密结构的形成
公开(公告)号：WO9951625A9
公开(公告)日：2001-07-05
申请号：PCT/US9907374
申请日：1999-04-02
申请人： RIGEL PHARMACEUTICALS INC
发明人： ANDERSON DAVID , GURURAJA TARIKERE
IPC分类号： C12N15/09 , C07K1/04 , C07K7/06 , C07K14/00 , C07K14/47 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P21/02 , C12Q1/02 , C12Q1/68
CPC分类号： C07K7/06 , C07K1/047 , C07K14/47
摘要： The present invention is directed to compositions and methods comprising peptides which have a high affinity for each other and, when linked to a protein, are used to help fold the protein into a compact structure. By virtue of its stability and constraints, this scaffold can prolong the activity of any embedded protein sequences in the presence of cellular and other proteases. The compact structure can have other functional sequences embedded, and is preferable to linear and less constrained peptides for library screening, for creating structurally-biased peptide libraries and for targeting to specific intracellular and extracellular compartments. Compositions of the present invention can be displayed on the surface of viruses, archaebacteria, prokaryotic and eukaryotic cells for library screening, drug screening and display. Methods of the present invention are useful for screening in vivo for intracellular effector proteins modulating signaling pathways and to identify interacting proteins in vitro. Thus, the present invention is useful as a scaffold for gene therapy, for the isolation of new therapeutic drug leads and for potential use as a therapeutic in physiological fluids.
摘要翻译：本发明涉及包含彼此具有高亲和力并且当与蛋白质连接时用于帮助将蛋白质折叠成紧密结构的肽的组合物和方法。由于其稳定性和约束，该支架可以延长任何嵌入的蛋白质序列在细胞和其它蛋白酶存在下的活性。紧密结构可以具有嵌入的其它功能序列，并且优选用于文库筛选的线性和较不受约束的肽，用于产生结构偏向肽文库并靶向特定的细胞内和细胞外区室。本发明的组合物可以显示在病毒，古细菌，原核和真核细胞的表面上，用于文库筛选，药物筛选和显示。本发明的方法可用于体内筛选调节信号传导途径的细胞内效应蛋白，并在体外鉴定相互作用蛋白。因此，本发明可用作基因治疗的支架，用于分离新的治疗药物引线和潜在用作生理液体中的治疗剂。

2. 发明申请

WO0020574A2 FUSIONS OF SCAFFOLD PROTEINS WITH RANDOM PEPTIDE LIBRARIES 审中-公开
标题翻译： SCAFF蛋白与随体肽库的融合
公开(公告)号：WO0020574A2
公开(公告)日：2000-04-13
申请号：PCT/US9923715
申请日：1999-10-08
申请人： RIGEL PHARMACEUTICALS INC
发明人： ANDERSON DAVID , BOGENBERGER JAKOB MARIA , PEELLE BEAU ROBERT
IPC分类号： C12N15/00 , C07K14/435 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N7/00 , C12N7/01 , C12N9/02 , C12N9/86 , C12N15/10 , C12N15/12 , C12N15/62 , C12Q1/68
CPC分类号： C12N15/1044 , C07K14/43595 , C07K2319/00 , C07K2319/42 , C07K2319/60 , C07K2319/70 , C12N15/1034 , C12N15/1086 , C12N15/62 , C12Q1/6897
摘要： The invention relates to the use of scaffold proteins, particularly green fluorescent protein (GFP), in fusion constructs with random and defined peptides and peptide libraries, to increase the cellular expression levels, decrease the cellular catabolism, increase the conformational stability relative to linear peptides, and to increase the steady state concentrations of the random peptides and random peptide library members expressed in cells for the purpose of detecting the presence of the peptides and screening random peptide libraries. N-terminal, C-terminal, dual N- and C- terminal and one or more internal fusions are all contemplated. Novel fusions utilizing self-binding peptides to create a conformationally stabilized fusion domain are also contemplated.
摘要翻译：本发明涉及在具有随机和定义的肽和肽文库的融合构建体中使用支架蛋白，特别是绿色荧光蛋白（GFP），以增加细胞表达水平，减少细胞分解代谢，增加相对于线性肽的构象稳定性并且增加在细胞中表达的随机肽和随机肽文库成员的稳态浓度，以便检测肽的存在和筛选随机肽文库。 N-末端，C-末端，双N-和C-末端以及一个或多个内部融合物都被考虑。还考虑利用自身结合肽产生构象稳定的融合结构域的新型融合体。

3. 发明公开

EP1399547A4 METHODS AND COMPOSITIONS COMPRISING i RENILLA /i GFP 审中-公开
标题翻译：方法和组合物与我海肾/ iGFP
公开(公告)号：EP1399547A4
公开(公告)日：2005-08-10
申请号：EP02729167
申请日：2002-05-09
申请人： RIGEL PHARMACEUTICALS INC
发明人： ANDERSON DAVID
IPC分类号： C07K14/435 , C12N15/62 , C12N15/867 , C12N15/00 , C07H21/04 , C07K1/00 , C12N15/01 , C12N15/63 , C12Q1/68
CPC分类号： C12N15/62 , C07K14/43595 , C07K2319/00 , C07K2319/23 , C07K2319/42 , C07K2319/43 , C07K2319/50 , C07K2319/60 , C12N15/86 , C12N2740/13043 , C12N2799/027
摘要： The invention relates to methods and compositions utilizing Renilla green fluorescent proteins (rGFP), and Ptilosarcus green fluorescent proteins (pGFP). In particular, the invention relates to the use of Renilla GFP or Ptilosarcus GFP proteins as reporters for cell assays, particularly intracellular assays, including methods of screening librairies, using rGFP or pGFP.

4. 发明申请

WO9942586A3 EXO1 AND EXO2, EXOCYTOTIC PROTEINS 审中-公开
标题翻译： EXO1和EXO2，EXOCYTOTIC蛋白
公开(公告)号：WO9942586A3
公开(公告)日：2000-02-24
申请号：PCT/US9903909
申请日：1999-02-23
申请人： RIGEL PHARMACEUTICALS INC
发明人： FISHER JOSEPH , LORENS JAMES , ANDERSON DAVID , LUO YING , HUANG BETTY , SHEN MARY
IPC分类号： G01N33/50 , C07K14/47 , C07K16/18 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/12 , C12P21/02 , C12Q1/02 , C12Q1/68
CPC分类号： C07K14/4703
摘要： The present invention is directed to novel exocytotic polypeptides, such as Exo1 and Exo2 polypeptides and related molecules, which have an inhibitory effect on exocytosis and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are methods for identifying novel compositions which mediate exocytotic polypeptide bioactivity, and the use of such compositions in diagnosis and treatment of disease.
摘要翻译：本发明涉及新颖的胞吐多肽，例如Exo1和Exo2多肽及相关分子，其对胞吐作用具有抑制作用，以及编码这些多肽的核酸分子。本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法发明。本发明进一步提供的是用于鉴定介导胞吐多肽生物活性的新组合物的方法，以及这些组合物在疾病诊断和治疗中的用途。

5. 发明申请

WO0134824A3 METHODS AND COMPOSITIONS COMPRISING RENILLA GFP 审中-公开
标题翻译：包含RENILLA GFP的方法和组合物
公开(公告)号：WO0134824A3
公开(公告)日：2002-05-02
申请号：PCT/US0030915
申请日：2000-11-10
申请人： RIGEL PHARMACEUTICALS INC
发明人： PEELLE BEAU ROBERT , ANDERSON DAVID
IPC分类号： G01N33/50 , C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/62 , C12N15/867 , C12Q1/02 , G01N33/15 , G01N33/53 , C12N15/86
CPC分类号： C12N15/86 , C07K14/43595 , C07K2319/00 , C07K2319/23 , C07K2319/42 , C07K2319/43 , C07K2319/50 , C07K2319/60 , C12N15/62 , C12N2740/13043
摘要： The invention relates to methods and compositions utilizing Renilla green fluorescent proteins (p- or rGFP). In particular, the invention relates to the use of Renilla GFP proteins as reporters for cell assays, particularly intracellular assays, including methods of screening libraries using p- or rGFP.
摘要翻译：本发明涉及利用海因绿色荧光蛋白（p-或rGFP）的方法和组合物。特别地，本发明涉及使用Renilla GFP蛋白作为细胞测定的记录，特别是细胞内测定法，包括使用p-或rGFP筛选文库的方法。

6. 发明申请

WO2004020458A3 ASSAYS AND COMPOSITIONS FOR IDENTIFYING AGENTS THAT MODULATE THE ACTIVITY OF DEUBIQUITINATING AGENTS 审中-公开
标题翻译：用于鉴定调节代谢试剂活性的试剂和组合物
公开(公告)号：WO2004020458A3
公开(公告)日：2004-11-11
申请号：PCT/US0327348
申请日：2003-08-29
申请人： RIGEL PHARMACEUTICALS INC , ANDERSON DAVID , HUANG JIANING , PRAY TODD R , WONG BRIAN
发明人： ANDERSON DAVID , HUANG JIANING , PRAY TODD R , WONG BRIAN
IPC分类号： C07K20060101 , C12Q1/37 , C12Q1/68 , G01N33/50 , G01N33/53 , G01N33/542 , G01N33/68
CPC分类号： G01N33/505 , G01N33/5008 , G01N33/5011 , G01N33/5017 , G01N33/502 , G01N33/5047 , G01N33/5064 , G01N33/542 , G01N33/68
摘要： Provided are methods and compositions for assaying for deubiquitinating agents that are enzymatic components of ubiquitin-mediated proteolysis and, their function, and agents that modulate the activity of such deubiquitinating agents.
摘要翻译：提供了用于测定作为泛素介导的蛋白水解的酶组分的去泛素化试剂的方法和组合物及其功能，以及调节这种去泛素化剂的活性的试剂。

7. 发明申请

WO0020574A9 FUSIONS OF SCAFFOLD PROTEINS WITH RANDOM PEPTIDE LIBRARIES 审中-公开
标题翻译： SCAFF蛋白与随体肽库的融合
公开(公告)号：WO0020574A9
公开(公告)日：2001-06-21
申请号：PCT/US9923715
申请日：1999-10-08
申请人： RIGEL PHARMACEUTICALS INC
发明人： ANDERSON DAVID , BOGENBERGER JAKOB MARIA , PEELLE BEAU ROBERT
IPC分类号： C12N15/00 , C07K14/435 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N7/00 , C12N7/01 , C12N9/02 , C12N9/86 , C12N15/10 , C12N15/12 , C12N15/62 , C12Q1/68
CPC分类号： C12N15/1044 , C07K14/43595 , C07K2319/00 , C07K2319/42 , C07K2319/60 , C07K2319/70 , C12N15/1034 , C12N15/1086 , C12N15/62 , C12Q1/6897
摘要： The invention relates to the use of scaffold proteins, particularly green fluorescent protein (GFP), in fusion constructs with random and defined peptides and peptide libraries, to increase the cellular expression levels, decrease the cellular catabolism, increase the conformational stability relative to linear peptides, and to increase the steady state concentrations of the random peptides and random peptide library members expressed in cells for the purpose of detecting the presence of the peptides and screening random peptide libraries. N-terminal, C-terminal, dual N- and C- terminal and one or more internal fusions are all contemplated. Novel fusions utilizing self-binding peptides to create a conformationally stabilized fusion domain are also contemplated.
摘要翻译：本发明涉及在具有随机和定义的肽和肽文库的融合构建体中使用支架蛋白，特别是绿色荧光蛋白（GFP），以增加细胞表达水平，减少细胞分解代谢，增加相对于线性肽的构象稳定性并且增加在细胞中表达的随机肽和随机肽文库成员的稳态浓度，以便检测肽的存在和筛选随机肽文库。 N-末端，C-末端，双N-和C-末端以及一个或多个内部融合物都被考虑。还考虑利用自身结合肽产生构象稳定的融合结构域的新型融合体。

8. 发明申请

WO9951625A2 PEPTIDES CAUSING FORMATION OF COMPACT STRUCTURES 审中-公开
标题翻译：导致紧密结构的形成
公开(公告)号：WO9951625A2
公开(公告)日：1999-10-14
申请号：PCT/US9907374
申请日：1999-04-02
申请人： RIGEL PHARMACEUTICALS INC
发明人： ANDERSON DAVID
IPC分类号： C12N15/09 , C07K1/04 , C07K7/06 , C07K14/00 , C07K14/47 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P21/02 , C12Q1/02 , C12Q1/68 , C07K7/00
CPC分类号： C07K7/06 , C07K1/047 , C07K14/47
摘要： The present invention is directed to compositions and methods comprising peptides which have a high affinity for each other and, when linked to a protein, are used to help fold the protein into a compact structure. By virtue of its stability and constraints, this scaffold can prolong the activity of any embedded protein sequences in the presence of cellular and other proteases. The compact structure can have other functional sequences embedded, and is preferable to linear and less constrained peptides for library screening, for creating structurally-biased peptide libraries and for targeting to specific intracellular and extracellular compartments. Compositions of the present invention can be displayed on the surface of viruses, archaebacteria, prokaryotic and eukaryotic cells for library screening, drug screening and display. Methods of the present invention are useful for screening in vivo for intracellular effector proteins modulating signaling pathways and to identify interacting proteins in vitro. Thus, the present invention is useful as a scaffold for gene therapy, for the isolation of new therapeutic drug leads and for potential use as a therapeutic in physiological fluids.
摘要翻译：本发明涉及包含彼此具有高亲和力并且当与蛋白质连接时用于帮助将蛋白质折叠成紧密结构的肽的组合物和方法。由于其稳定性和约束，该支架可以延长任何嵌入的蛋白质序列在细胞和其它蛋白酶存在下的活性。紧密结构可以具有嵌入的其它功能序列，并且优选用于文库筛选的线性和较不受约束的肽，用于产生结构偏向肽文库并靶向特定的细胞内和细胞外区室。本发明的组合物可以显示在病毒，古细菌，原核和真核细胞的表面上，用于文库筛选，药物筛选和显示。本发明的方法可用于体内筛选调节信号传导途径的细胞内效应蛋白，并在体外鉴定相互作用蛋白。因此，本发明可用作基因治疗的支架，用于分离新的治疗药物引线和潜在用作生理液体中的治疗剂。

9. 发明专利

AT362528T 未知
公开(公告)号：AT362528T
公开(公告)日：2007-06-15
申请号：AT99957466
申请日：1999-10-08
申请人： RIGEL PHARMACEUTICALS INC
发明人： ANDERSON DAVID , BOGENBERGER JAKOB , PEELLE BEAU
IPC分类号： C12N15/00 , C12N15/10 , C07K14/435 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N7/00 , C12N7/01 , C12N9/02 , C12N9/86 , C12N15/12 , C12N15/62 , C12Q1/68 , C12Q1/6897
摘要： The invention relates to the use of fluorescent proteins, particularly green fluorescent protein (GFP), in fusion constructs with random and defined peptides and peptide libraries, to increase the cellular expression levels, decrease the cellular catabolism, increase the conformational stability relative to linear peptides, and to increase the steady state concentrations of the random peptides and random peptide library members expressed in cells for the purpose of detecting the presence of the peptides and screening random peptide libraries. N-terminal, C-terminal, dual N- and C-terminal and one or more internal fusions are all contemplated. Novel fusions utilizing self-binding peptides to create a conformationally stabilized fusion domain are also contemplated.

10. 发明专利

AU2003290557A1 CELL CYCLE TARGETS AND PEPTIDES 未知
公开(公告)号：AU2003290557A1
公开(公告)日：2004-06-07
申请号：AU2003290557
申请日：2003-10-30
申请人： RIGEL PHARMACEUTICALS INC
发明人： HITOSHI YASUMICHI , ANDERSON DAVID
IPC分类号： C07K20060101
摘要： The present invention relates to regulation of cellular proliferation. More particularly, the present invention binding partners of peptides that block the cell cycle. These binding partners are targets for drug discovery, e.g., for small molecule cancer therapeutics.

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