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1. 发明授权

US10907181B2 Biosynthetic pathways and products 有权
公开(公告)号：US10907181B2
公开(公告)日：2021-02-02
申请号：US16722265
申请日：2019-12-20
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Kechun Zhang , Mingyong Xiong
IPC分类号： C07D309/38 , C12P17/06 , C12P7/42 , C12N9/10 , C07D309/30 , C07D309/32
摘要： This disclosure describes biosynthesized compounds including anhydromevalonolactone and β-methyl-δ-valerolactone. This disclosure further describes biosynthetic methods for making these compounds. In some embodiments, the biosynthetic methods can include a combination of biosynthesis and chemical steps to produce β-methyl-δ-valerolactone. Finally, this disclosure described recombinant cells useful for the biosynthesis of these compounds.

2. 发明授权

US10006064B2 Biosynthetic pathways, recombinant cells, and methods 有权
公开(公告)号：US10006064B2
公开(公告)日：2018-06-26
申请号：US14404125
申请日：2013-03-14
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Kechun Zhang , Mingyong Xiong , Yi-Shu Tai
IPC分类号： C12P7/62 , C12N9/10
CPC分类号： C12P7/62 , C12N9/1029
摘要： This disclosure describes engineered biosynthetic pathways, recombinant cells, and methods relating to biosynthesis of esters. The recombinant cells may be modified to exhibit increased biosynthesis of an ester compared to a wild-type control. The recombinant cell may be incubated in medium that includes a carbon source under conditions effective for the recombinant cell to produce an ester. This disclosure also describes a method that generally includes introducing into a host cell a heterologous polynucleotide encoding at least one polypeptide that catalyzes a step in converting a carbon source to an ester, wherein the at least one polynucleotide is operably linked to a promoter so that the modified host cell catalyzes conversion of the carbon source to an ester.

3. 发明申请

US20150140620A1 BIOSYNTHETIC PATHWAYS, RECOMBINANT CELLS, AND METHODS 有权
标题翻译：生物路径，重组细胞和方法
公开(公告)号：US20150140620A1
公开(公告)日：2015-05-21
申请号：US14404125
申请日：2013-03-14
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Kechun Zhang , Mingyong Xiong , Yi-Shu Tai
IPC分类号： C12P7/62 , C12N9/10
CPC分类号： C12P7/62 , C12N9/1029
摘要： This disclosure describes engineered biosynthetic pathways, recombinant cells, and methods relating to biosynthesis of esters. The recombinant cells may be modified to exhibit increased biosynthesis of an ester compared to a wild-type control. The recombinant cell may be incubated in medium that includes a carbon source under conditions effective for the recombinant cell to produce an ester. This disclosure also describes a method that generally includes introducing into a host cell a heterologous polynucleotide encoding at least one polypeptide that catalyzes a step in converting a carbon source to an ester, wherein the at least one polynucleotide is operably linked to a promoter so that the modified host cell catalyzes conversion of the carbon source to an ester.
摘要翻译：本公开描述了工程化生物合成途径，重组细胞和与酯的生物合成相关的方法。与野生型对照相比，重组细胞可以被修饰以显示酯的增加的生物合成。可以在对重组细胞有效产生酯的条件下，将包含碳源的培养基中培养重组细胞。本公开还描述了一种方法，其通常包括向宿主细胞中引入编码至少一种多肽的异源多核苷酸，所述多核苷酸催化将碳源转化为酯的步骤，其中所述至少一种多核苷酸可操作地连接至启动子，使得改性宿主细胞催化碳源向酯的转化。

4. 发明申请

US20150132813A1 BIOSYNTHETIC PATHWAYS, RECOMBINANT CELLS, AND METHODS 审中-公开
标题翻译：生物路径，重组细胞和方法
公开(公告)号：US20150132813A1
公开(公告)日：2015-05-14
申请号：US14399681
申请日：2013-03-13
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Kechun Zhang , Yogesh K. Dhande
IPC分类号： C12P7/40 , C12N9/02 , C12N9/88
CPC分类号： C12P7/40 , C12N9/0004 , C12N9/0008 , C12N9/88 , C12Y102/01003 , C12Y401/01
摘要： This disclosure describes, generally, recombinant cells modified to exhibit increased biosynthesis of pentanoic acid, methods of making such recombinant cells, and methods of inducing the cells to produce pentanoic acid. This disclosure also describes, generally, recombinant cells modified to exhibit increased biosynthesis of 2-methylbutyric acid, methods of making such recombinant cells, and methods of inducing the cells to produce 2-methylbutyric acid.
摘要翻译：本公开通常描述了修饰以显示戊酸生物合成增加的重组细胞，制备这种重组细胞的方法，以及诱导细胞产生戊酸的方法。本公开通常还描述了修饰以显示2-甲基丁酸的增加的生物合成的重组细胞，制备这种重组细胞的方法，以及诱导细胞产生2-甲基丁酸的方法。

5. 发明授权

US10414858B2 Methods of forming diol compounds 有权
公开(公告)号：US10414858B2
公开(公告)日：2019-09-17
申请号：US15491236
申请日：2017-04-19
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Paul J. Dauenhauer , Charles Spanjers , Kechun Zhang
IPC分类号： C07C29/151 , C08G63/85 , C07C29/149
摘要： Methods of forming a C4 to C7 diol compound, the methods including a first step of reacting a C4 to C7 dicarboxylic acid with hydrogen (H2) gas on a first heterogeneous catalyst at a first temperature and a first pressure to form a C4 to C7 lactone; and a subsequent step of reacting the lactone with hydrogen (H2) gas on a second heterogeneous catalyst at a second temperature and a second pressure, wherein the second temperature is lower than the first temperature. Also disclosed are methods of forming a solvent, the methods including reacting a C4 to C7 dicarboxylic acid with hydrogen (H2) gas on a first heterogeneous catalyst at a first temperature and a first pressure to form a solvent. Further disclosed herein are methods that include reacting mevalonolactone with hydrogen (H2) gas on a second heterogeneous catalyst at a second temperature and a second pressure to form a diol compound.

6. 发明授权

US09909129B2 Biosynthetic pathways and methods 有权
公开(公告)号：US09909129B2
公开(公告)日：2018-03-06
申请号：US14653118
申请日：2013-12-18
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Kechun Zhang , Mingyong Xiong
IPC分类号： C12P7/18 , C12N9/02 , C12N9/88 , C12N9/04 , C12N15/52 , C12N9/18
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/18 , C12N9/88 , C12P7/18 , C12Y101/01 , C12Y101/01001 , C12Y102/01026 , C12Y301/01 , C12Y301/01068 , C12Y401/01007 , C12Y402/00 , C12Y402/01082
摘要： This disclosure describes a recombinant microbial cells and methods of making and using such recombinant microbial cells. Generally, the recombinant cells may be modified to exhibit increased biosynthesis of a TCA derivative compared to a wild-type control. In some embodiments, the TCA derivative can include 1,4-butanediol. In various embodiments, the microbial cell is a fungal cell or a bacterial cell. In some embodiments, the increased biosynthesis of the TCA derivative can include an increase in xylose dehydrogenase activity, xylonolactonase activity, xylonate dehydratase activity, or 2-keto-3-deoxyaldonic acid dehydratase activity.

7. 发明申请

US20170297983A1 METHODS OF FORMING DIOL COMPOUNDS 审中-公开
公开(公告)号：US20170297983A1
公开(公告)日：2017-10-19
申请号：US15491236
申请日：2017-04-19
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Paul J. Dauenhauer , Charles Spanjers , Kechun Zhang
IPC分类号： C07C29/151 , C08G63/85
CPC分类号： C08G63/85 , C07C29/149 , C07C31/20 , C07C31/22
摘要： Methods of forming a C4 to C7 diol compound, the methods including a first step of reacting a C4 to C7 dicarboxylic acid with hydrogen (H2) gas on a first heterogeneous catalyst at a first temperature and a first pressure to form a C4 to C7 lactone; and a subsequent step of reacting the lactone with hydrogen (H2) gas on a second heterogeneous catalyst at a second temperature and a second pressure, wherein the second temperature is lower than the first temperature. Also disclosed are methods of forming a solvent, the methods including reacting a C4 to C7 dicarboxylic acid with hydrogen (H2) gas on a first heterogeneous catalyst at a first temperature and a first pressure to form a solvent. Further disclosed herein are methods that include reacting mevalonolactone with hydrogen (H2) gas on a second heterogeneous catalyst at a second temperature and a second pressure to form a diol compound.

8. 发明授权

US10202624B2 Biosynthetic pathways and products 有权
公开(公告)号：US10202624B2
公开(公告)日：2019-02-12
申请号：US14783375
申请日：2014-04-18
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Kechun Zhang , Mingyong Xiong
IPC分类号： C07D309/30 , C12P17/06 , C07D309/32 , C07D309/38 , C12P7/42 , C12N9/10
摘要： This disclosure describes biosynthesized compounds including anhydromevalonolactone and β-methyl-δ-valerolactone. This disclosure further describes biosynthetic methods for making these compounds. In some embodiments, the biosynthetic methods can include a combination of biosynthesis and chemical steps to produce β-methyl-δ-valerolactone. Finally, this disclosure described recombinant cells useful for the biosynthesis of these compounds.

9. 发明申请

US20170137855A1 RECOMBINANT CELLS AND METHODS FOR NONPHOSPHORYLATIVE METABOLISM 审中-公开
公开(公告)号：US20170137855A1
公开(公告)日：2017-05-18
申请号：US15353188
申请日：2016-11-16
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Kechun Zhang , Yi-shu Tai , Pooja Jambunathan
IPC分类号： C12P19/02 , C12P7/40 , C12N9/88 , C12N9/04 , C12N9/18
CPC分类号： C12Y402/01025 , C12N9/0006 , C12N9/18 , C12N9/88 , C12P7/18 , C12P7/40 , C12P7/44 , C12Y101/01046 , C12Y301/01015 , C12Y402/01043
摘要： This disclosure describes methods for screening heterologous coding regions (e.g., a single gene coding region, and operon, or other gene cluster) for the ability to genetically modify a host cell to perform nonphosphorylative biosynthesis of 2,5-dioxopentanoate and downstream derivatives thereof (e.g., 1,4-butanediol, glutamate, or glutaconate. This disclosure further describes recombinant cells modified to increase nonphosphorylative biosynthesis of 2,5-dioxopentanoate compared to an appropriate control cell (e.g., a wild-type cell or an otherwise genetically-modified cell) and methods of using making and using such recombinant cells.

10. 发明申请

US20160068877A1 BIOSYNTHETIC PATHWAYS AND PRODUCTS 审中-公开
标题翻译：生物路径和产品
公开(公告)号：US20160068877A1
公开(公告)日：2016-03-10
申请号：US14783375
申请日：2014-04-18
申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： Kechun Zhang , Mingyong Xiong
IPC分类号： C12P17/06 , C07D309/32 , C07D309/30 , C12P7/42
CPC分类号： C12P17/06 , C07D309/30 , C07D309/32 , C07D309/38 , C12N9/1025 , C12N9/1029 , C12P7/42
摘要： This disclosure describes biosynthesized compounds including anhydromevalonolactone and β-methyl-δ-valerolactone. This disclosure further describes biosynthetic methods for making these compounds. In some embodiments, the biosynthetic methods can include a combination of biosynthesis and chemical steps to produce β-methyl-δ-valerolactone. Finally, this disclosure described recombinant cells useful for the biosynthesis of these compounds.
摘要翻译：本公开描述了生物合成化合物，包括氢化戊内酯和β-甲基-δ-戊内酯。本公开进一步描述了制备这些化合物的生物合成方法。在一些实施方案中，生物合成方法可以包括生物合成和化学步骤的组合以产生β-甲基-δ-戊内酯。最后，本公开描述了可用于这些化合物的生物合成的重组细胞。

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