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1. 发明申请

WO2005070966A2 FUSION POLYPEPTIDES CAPABLE OF ACTIVATING RECEPTORS 审中-公开
标题翻译：可激活受体的融合多肽
公开(公告)号：WO2005070966A2
公开(公告)日：2005-08-04
申请号：PCT/US2005/001246
申请日：2005-01-14
申请人： REGENERON PHARMACEUTICALS, INC. , FANDL, James , CHEN, Gang , PAPADOPOULOS, Nicholas , ALDRICH, Thomas, H.
发明人： FANDL, James , CHEN, Gang , PAPADOPOULOS, Nicholas , ALDRICH, Thomas, H.
IPC分类号： C07K16/28
CPC分类号： C07K16/2863 , C07H21/04 , C07K2317/31 , C07K2317/52 , C07K2317/622 , C07K2317/75 , C07K2319/30
摘要： A fusion polypeptide comprising (A) x -M-(A’) Y , wherein A and A’ are each polypeptides capable of binding a target receptor. The fusion polypeptides of the invention form multimeric proteins which activate the target receptor. A and A’ may be each be an antibody or fragment derived form an antibody specific for a target receptor,, such as the same or different ScFv fragments, and/or a ligand or ligand fragment or derivative capable of binding the target protein, M is a multimerizing component, and X and Y are independently a number between 1-10.
摘要翻译：包含（A）x-M-（A'）Y的融合多肽，其中A和A'是能够结合靶受体的多肽。本发明的融合多肽形成激活靶受体的多聚体蛋白。 A和A'可以各自是抗体或衍生自对靶受体特异性的抗体，例如相同或不同的ScFv片段，和/或能够结合靶蛋白的配体或配体片段或衍生物，M 是多聚成分，X和Y独立地为1-10之间的数。

2. 发明申请

WO2016028838A1 EFFICIENT SELECTIVITY OF RECOMBINANT PROTEINS 审中-公开
标题翻译：重组蛋白的有效选择性
公开(公告)号：WO2016028838A1
公开(公告)日：2016-02-25
申请号：PCT/US2015/045793
申请日：2015-08-19
申请人： REGENERON PHARMACEUTICALS, INC. , DESHPANDE, Dipali , BURAKOV, Darya , CHEN, Gang , FANDL, James
发明人： DESHPANDE, Dipali , BURAKOV, Darya , CHEN, Gang , FANDL, James
IPC分类号： C12P21/06 , C12N15/00 , C12N5/07 , C12N5/10 , C12N5/02 , C12N9/12
CPC分类号： C12N15/85 , C07K2319/30 , C12P21/005
摘要： The invention provides a new expression system comprising a mammalian selectable marker that promotes desirable post-translational modifications of glycoproteins. In particular, the invention includes methods and compositions for optimal recombinant protein expression in mammalian cells by employing a selection marker system based on GPT genes of mammalian origin. The invention includes methods that facilitate selectivity and enhanced expression copies as well as protein yield of recombinant proteins in mammalian cells, and methods of using GPT expression systems.
摘要翻译：本发明提供了一种新的表达系统，其包括促进糖蛋白的期望的翻译后修饰的哺乳动物选择性标记。特别地，本发明包括通过使用基于哺乳动物来源的GPT基因的选择标记系统来在哺乳动物细胞中优化重组蛋白表达的方法和组合物。本发明包括促进哺乳动物细胞中重组蛋白质的选择性和增强的表达拷贝以及蛋白质产量的方法，以及使用GPT表达系统的方法。

3. 发明申请

WO2014078475A3 RECOMBINANT CELL SURFACE CAPTURE PROTEINS 审中-公开
公开(公告)号：WO2014078475A3
公开(公告)日：2014-05-22
申请号：PCT/US2013/069993
申请日：2013-11-14
申请人： REGENERON PHARMACEUTICALS, INC.
发明人： DESHPANDE, Dipali , CHEN, Gang , BURAKOV, Darya , FANDL, James , ALDRICH, Thomas , KAMAT, Vishal
IPC分类号： C07K16/18 , C07K16/42 , C12N15/10
摘要： Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and/or substituted CH3 domain are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and/or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).

4. 发明授权

EP2949667B1 RECOMBINANT CELL SURFACE CAPTURE PROTEINS 有权
公开(公告)号：EP2949667B1
公开(公告)日：2020-05-13
申请号：EP15172149.5
申请日：2013-11-14
申请人： Regeneron Pharmaceuticals, Inc.
发明人： DESHPANDE, Dipali , CHEN, Gang , BURAKOV, Darya , FANDL, James , ALDRICH, Thomas , KAMAT, Vishal
IPC分类号： C07K16/18 , C07K16/42 , C12N15/10 , C07K16/22 , C07K16/28

5. 发明公开

EP3901274A1 EFFICIENT SELECTIVITY OF RECOMBINANT PROTEINS 审中-公开
公开(公告)号：EP3901274A1
公开(公告)日：2021-10-27
申请号：EP20211902.0
申请日：2015-08-19
申请人： Regeneron Pharmaceuticals, Inc.
发明人： DESHPANDE, Dipali , BURAKOV, Darya , CHEN, Gang , FANDL, James
IPC分类号： C12P21/06 , C12N15/00 , C12P21/00 , C12N15/85
摘要： The invention provides a new expression system comprising a mammalian selectable marker (tunicamycin-resistant UDP-N-acetylglucosamine: dolichol phosphateN-acetylglucosamine-1-P transferase) that promotes desirable post-translational modifications of glycoproteins. In particular, the invention includes methods and compositions for optimal recombinant protein expression in mammalian cells by employing a selection marker system based on GPT genes of mammalian origin. The invention includes methods that facilitate selectivity and enhanced expression copies as well as protein yield of recombinant proteins in mammalian cells, and methods of using GPT expression systems.

6. 发明公开

EP2920208A2 RECOMBINANT CELL SURFACE CAPTURE PROTEINS 有权
标题翻译：重组细胞表面捕获蛋白
公开(公告)号：EP2920208A2
公开(公告)日：2015-09-23
申请号：EP13798483.7
申请日：2013-11-14
申请人： Regeneron Pharmaceuticals, Inc.
发明人： DESHPANDE, Dipali , CHEN, Gang , BURAKOV, Darya , FANDL, James , ALDRICH, Thomas , KAMAT, Vishal
IPC分类号： C07K16/18 , C07K16/42 , C12N15/10
摘要： Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and/or substituted CH3 domain are provided. Methods for detecting and isolating cells expressing heterodimeric proteins are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and/or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).
摘要翻译：提供了用于分离和检测产生具有免疫球蛋白CH3结构域和/或取代CH3结构域的分泌型异源二聚体蛋白质（POI）的细胞的重组细胞表面捕获蛋白和检测分子。还提供了分离和检测双特异性抗体的重组细胞表面捕获蛋白和检测分子。本发明还提供能够识别和结合包含CH3结构域和/或修饰的CH3结构域的感兴趣的蛋白质的重组抗原结合蛋白，所述CH3结构域例如在H95和Y96处具有或不具有氨基酸取代的CH3结构域（IMGT ）。

7. 发明授权

EP2920208B1 RECOMBINANT CELL SURFACE CAPTURE PROTEINS 有权
公开(公告)号：EP2920208B1
公开(公告)日：2020-08-19
申请号：EP13798483.7
申请日：2013-11-14
申请人： Regeneron Pharmaceuticals, Inc.
发明人： DESHPANDE, Dipali , CHEN, Gang , BURAKOV, Darya , FANDL, James , ALDRICH, Thomas , KAMAT, Vishal
IPC分类号： C07K16/22 , C07K16/28 , C07K16/42

8. 发明公开

EP2949667A3 RECOMBINANT CELL SURFACE CAPTURE PROTEINS 有权
标题翻译： REKOMBINANTEZELLOBERFLÄCHENERFASSUNGSPROTEINE
公开(公告)号：EP2949667A3
公开(公告)日：2016-02-24
申请号：EP15172149.5
申请日：2013-11-14
申请人： Regeneron Pharmaceuticals, Inc.
发明人： DESHPANDE, Dipali , CHEN, Gang , BURAKOV, Darya , FANDL, James , ALDRICH, Thomas , KAMAT, Vishal
IPC分类号： C07K16/18 , C07K16/42 , C12N15/10 , C07K16/22 , C07K16/28
摘要： Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and/or substituted CH3 domain are provided. Methods for detecting and isolating cells expressing heterodimeric proteins are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and/or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).
摘要翻译：提供了可用于分离和检测产生具有免疫球蛋白CH3结构域和/或取代的CH3结构域的目标分泌异二聚体蛋白质（POI）的细胞的重组细胞表面捕获蛋白和检测分子。提供了检测和分离表达异二聚体蛋白的细胞的方法。还提供了分离和检测双特异性抗体的重组细胞表面捕获蛋白和检测分子。本发明还提供重组抗原结合蛋白，其能够识别和结合含有CH3结构域和/或修饰的CH3结构域的目标蛋白，例如在H95和Y96处有或没有氨基酸取代的CH3结构域（IMGT ）。

9. 发明公开

EP2949667A2 RECOMBINANT CELL SURFACE CAPTURE PROTEINS 有权
标题翻译：重组细胞表面识别蛋白
公开(公告)号：EP2949667A2
公开(公告)日：2015-12-02
申请号：EP15172149.5
申请日：2013-11-14
申请人： Regeneron Pharmaceuticals, Inc.
发明人： DESHPANDE, Dipali , CHEN, Gang , BURAKOV, Darya , FANDL, James , ALDRICH, Thomas , KAMAT, Vishal
IPC分类号： C07K16/18 , C07K16/42 , C12N15/10 , C07K16/22 , C07K16/28
摘要： Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and/or substituted CH3 domain are provided. Methods for detecting and isolating cells expressing heterodimeric proteins are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and/or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).

10. 发明公开

EP3183359A1 EFFICIENT SELECTIVITY OF RECOMBINANT PROTEINS 有权
标题翻译：重组蛋白的有效选择性
公开(公告)号：EP3183359A1
公开(公告)日：2017-06-28
申请号：EP15833421.9
申请日：2015-08-19
申请人： Regeneron Pharmaceuticals, Inc.
发明人： DESHPANDE, Dipali , BURAKOV, Darya , CHEN, Gang , FANDL, James
IPC分类号： C12P21/06 , C12N15/00 , C12N5/07 , C12N5/10 , C12N5/02 , C12N9/12
CPC分类号： C12N15/85 , C07K2319/30 , C12P21/005
摘要： The invention provides a new expression system comprising a mammalian selectable marker that promotes desirable post-translational modifications of glycoproteins. In particular, the invention includes methods and compositions for optimal recombinant protein expression in mammalian cells by employing a selection marker system based on GPT genes of mammalian origin. The invention includes methods that facilitate selectivity and enhanced expression copies as well as protein yield of recombinant proteins in mammalian cells, and methods of using GPT expression systems.
摘要翻译：本发明提供了新的表达系统，其包含促进糖蛋白的理想的翻译后修饰的哺乳动物选择标记。具体而言，本发明包括通过使用基于哺乳动物来源的GPT基因的选择标记系统在哺乳动物细胞中优化重组蛋白表达的方法和组合物。本发明包括促进哺乳动物细胞中重组蛋白质的选择性和增强的表达拷贝以及蛋白质产量的方法，以及使用GPT表达系统的方法。

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