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1. 发明申请

US20120088243A1 METHOD FOR DETECTION OF GENETICALLY MODIFIED MAIZE BT11 失效
标题翻译：用于检测遗传改良玉米BT11的方法
公开(公告)号：US20120088243A1
公开(公告)日：2012-04-12
申请号：US12673322
申请日：2009-04-17
申请人： Qingkuo Lan , Yong Wang , Yi Cheng , Xin Zhao , Zhu Zhu
发明人： Qingkuo Lan , Yong Wang , Yi Cheng , Xin Zhao , Zhu Zhu
IPC分类号： C12Q1/68 , G01N33/559
CPC分类号： C12N15/8286 , A01H1/04 , C12Q1/6895 , C12Q2600/156 , Y02A40/162
摘要： The invention discloses a method for detection of genetically modified maize BT11. The principle of the method is that the DNA template of the sample is amplified at a temperature of 63° C.˜65° C. for 45˜60 min by using 4 specific primers and a DNA polymerase with strand displacement activity. The identification thereof is to make a judgment on whether BT11 component is contained in the sample by directly observing the turbidity in the reaction tube or the color change after the addition of SYBR Green with naked eyes or by agarose gel electrophoresis. The detection method of the invention has the advantages of high specificity, quickness, simplicity and convenience and the like, which provides a convenient method for detection of genetically modified maize BT11 with an extensive application prospect.
摘要翻译：本发明公开了一种检测转基因玉米BT11的方法。该方法的原理是通过使用4种特异性引物和具有链置换活性的DNA聚合酶，在63℃〜65℃的温度下扩增样品的DNA模板45〜60分钟。其鉴别是通过直接观察反应管中的浊度或用肉眼添加SYBR Green后的颜色变化或通过琼脂糖凝胶电泳来判断样品中BT11成分是否包含在样品中。本发明的检测方法具有特异性高，快速，简便，方便等优点，为广泛应用前景的转基因玉米BT11检测提供了便捷的方法。

2. 发明授权

US08367341B2 Method for detection of genetically modified maize BT11 失效
标题翻译：转基因玉米BT11检测方法
公开(公告)号：US08367341B2
公开(公告)日：2013-02-05
申请号：US12673322
申请日：2009-04-17
申请人： Qingkuo Lan , Yong Wang , Yi Cheng , Xin Zhao , Zhu Zhu
发明人： Qingkuo Lan , Yong Wang , Yi Cheng , Xin Zhao , Zhu Zhu
IPC分类号： C12Q1/68 , C12P19/34 , C07H1/00 , C07H5/04 , C07H19/00 , C07H21/02 , C07H21/04
CPC分类号： C12N15/8286 , A01H1/04 , C12Q1/6895 , C12Q2600/156 , Y02A40/162
摘要： The invention discloses a method for detection of genetically modified maize BT11. The principle of the method is that the DNA template of the sample is amplified at a temperature of 63° C.˜65° C. for 45˜60 min by using 4 specific primers and a DNA polymerase with strand displacement activity. The identification thereof is to make a judgment on whether BT11 component is contained in the sample by directly observing the turbidity in the reaction tube or the color change after the addition of SYBR Green with naked eyes or by agarose gel electrophoresis. The detection method of the invention has the advantages of high specificity, quickness, simplicity and convenience and the like, which provides a convenient method for detection of genetically modified maize BT11 with an extensive application prospect.
摘要翻译：本发明公开了一种检测转基因玉米BT11的方法。该方法的原理是通过使用4种特异性引物和具有链置换活性的DNA聚合酶，在63℃〜65℃的温度下扩增样品的DNA模板45〜60分钟。其鉴别是通过直接观察反应管中的浊度或用肉眼添加SYBR Green后的颜色变化或通过琼脂糖凝胶电泳来判断样品中BT11成分是否包含在样品中。本发明的检测方法具有特异性高，快速，简便，方便等优点，为广泛应用前景的转基因玉米BT11检测提供了便捷的方法。

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