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1. 发明申请

WO2004108741A1 PROCESS FOR THE CONCENTRATION AND/OR ISOLATION OF NUCLEIC ACID OR NUCLEIC ACID-CONTAINING SPECIES 审中-公开
标题翻译：浓缩和/或分离含有核酸或含核酸的物种的方法
公开(公告)号：WO2004108741A1
公开(公告)日：2004-12-16
申请号：PCT/EP2004/005998
申请日：2004-06-03
申请人： QIAGEN AS , QIAGEN GMBH , DEGGERDAL, Arne , REITAN, Evy, H. , SKAGESTAD, Vidar
发明人： DEGGERDAL, Arne , REITAN, Evy, H. , SKAGESTAD, Vidar
IPC分类号： C07H1/08
CPC分类号： C12N15/1003
摘要： The present invention relates to a process for the concentration and/or isolation of nucleic acids or nucleic acid-containing species from a nucleic acid-containing solution, and a kit therefor. In one embodiment, the invention relates to the concentration and/or isolation of DNA and RNA from nucleic acid-containing solutions.
摘要翻译：本发明涉及从含核酸的溶液中浓缩和/或分离核酸或含核酸物质的方法及其用途。在一个实施方案中，本发明涉及来自含核酸溶液的DNA和RNA的浓度和/或分离。

2. 发明申请

WO2004108925A1 METHOD FOR SEQUENTIALLY ISOLATING DNA AND RNA FROM THE SAME NUCLEIC ACID-CONTAINING SAMPLE 审中-公开
标题翻译：用于从相同的含有核酸的样品中顺序分离DNA和RNA的方法
公开(公告)号：WO2004108925A1
公开(公告)日：2004-12-16
申请号：PCT/EP2004/005999
申请日：2004-06-03
申请人： QIAGEN AS , QIAGEN GMBH , REITAN, Evy, H. , DEGGERDAL, Arne , SKAGESTAD, Vidar
发明人： REITAN, Evy, H. , DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C12N15/10
CPC分类号： C12N15/1003 , C12N15/1006
摘要： The present invention refers to a method for isolating nucleic acids from a nucleic acid-containing sample. More specifically, it relates to a method for sequentially isolating DNA and RNA from the same nucleic acid-containing sample. Furthermore, the present invention refers to kit for sequentially isolating DNA and RNA from the same nucleic acid-containing sample.
摘要翻译：本发明涉及从含核酸样品中分离核酸的方法。更具体地说，本发明涉及从相同的含有核酸的样品中顺序分离DNA和RNA的方法。此外，本发明涉及用于从相同的含有核酸的样品中顺序分离DNA和RNA的试剂盒。

3. 发明申请

WO2003095646A1 ISOLATING NUCLEIC ACID 审中-公开
标题翻译：分离核酸
公开(公告)号：WO2003095646A1
公开(公告)日：2003-11-20
申请号：PCT/IB2003/001822
申请日：2003-05-09
申请人： QIAGEN AS , DEGGERDAL, Arne , SKAGESTAD, Vidar
发明人： DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C12N15/10
CPC分类号： C12N15/1006
摘要： A process. for isolating nucleic acid from a nucleic acid containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase, wherein the solid phase bears acid groups on its surface.
摘要翻译：一个过程。用于从含有核酸的样品中分离核酸，其包括：（a）提供离液剂; （b）提供能够在离液剂存在下结合核酸的核酸结合固相; （c）在包含离液剂的液相存在下使样品与核酸结合固相接触; 和（e）任选地将所述固相与与所述液相结合的核酸分离，其中所述固相在其表面上具有酸性基团。

4. 发明申请

WO2003091452A1 ISOLATING NUCLEIC ACID 审中-公开
标题翻译：分离核酸
公开(公告)号：WO2003091452A1
公开(公告)日：2003-11-06
申请号：PCT/IB2003/001553
申请日：2003-04-22
申请人： QIAGEN AS , DEGGERDAL, Arne , SKAGESTAD, Vidar
发明人： DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C12Q1/68
CPC分类号： C12N15/1013 , C12Q1/6806 , C12Q2563/143 , C12Q2527/125
摘要： A process for isolating nucleic acid from a nucleic acid- containing biological sample, which comprises: (a) providing magnetic particles capable of binding nucleic acid; (b) contacting the sample with the magnetic particles so as to bind the nucleic acid thereto; (c) forming an aggregate of the magnetic particles by the application of a magnetic field, which aggregate comprises magnetic particles with the nucleic acid bound thereto and contaminants from the biological sample; (d) contacting the aggregate with a washing solution to disperse the aggregate and release the contaminants therefrom; and (e) separating therefrom the magnetic particles with the nucleic acid bound thereto; wherein the aggregate is formed in the presence of an amount of a water-miscible lower alcohol sufficient to facilitate dispersion of the aggregate and release of the contaminants therefrom when washed in step (d), with the proviso that, when the magnetic particles are silica-based magnetic glass particles provided with the water-miscible lower alcohol, the water-miscible lower alcohol is part of an aqueous mixture which further comprises a salt.
摘要翻译：一种用于从含核酸的生物样品中分离核酸的方法，其包括：（a）提供能够结合核酸的磁性颗粒; （b）使样品与磁性颗粒接触以使核酸与其结合; （c）通过施加磁场形成磁性颗粒的聚集体，该聚集体包含与其结合的核酸的磁性颗粒和来自生物样品的污染物; （d）使聚集体与洗涤溶液接触以分散聚集体并从其中释放污染物; 和（e）与其结合的核酸与其分离; 其中所述聚集体在一定量的水混溶性低级醇存在下形成，所述量可以在步骤（d）中洗涤时足以促进聚集体的分散和其中的污染物的释放，条件是当磁性颗粒是二氧化硅提供有水混溶性低级醇的基于水的磁性玻璃颗粒，水混溶性低级醇是进一步包含盐的含水混合物的一部分。

5. 发明申请

WO2003084976A1 PROCESS FOR ISOLATING NUCLEIC ACID WITH CHAOTROPE AGENTS AND AMMONIUM COMPOUNDS 审中-公开
标题翻译：用水解剂和氨基化合物分离核酸的方法
公开(公告)号：WO2003084976A1
公开(公告)日：2003-10-16
申请号：PCT/IB2003/001202
申请日：2003-04-02
申请人： QIAGEN AS , REITAN, Evy , DEGGERDAL, Arne , SKAGESTAD, Vidar
发明人： REITAN, Evy , DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C07H21/00
CPC分类号： C12N15/1006
摘要： A process for isolating nucleic acid from a nucleic acid-containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) providing a source of NH4+ or NH3; (d) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope and the NH4+ or NH3; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase.
摘要翻译：一种从含核酸样品中分离核酸的方法，包括：（a）提供离液剂; （b）提供能够在离液剂存在下结合核酸的核酸结合固相; （c）提供NH4 +或NH3的来源; （d）在包含离液剂和NH 4 +或NH 3的液相存在下使样品与核酸结合固相接触; 和（e）任选地将所述固相与与液相结合的核酸分离。

6. 发明申请

WO2009040444A1 RNA ISOLATION METHOD 审中-公开
标题翻译： RNA分离方法
公开(公告)号：WO2009040444A1
公开(公告)日：2009-04-02
申请号：PCT/EP2008/063039
申请日：2008-09-29
申请人： MOLE GENETICS AS , DEGGERDAL, Arne , CAMPBELL, Andrew , BOGER, Veronica
发明人： DEGGERDAL, Arne , CAMPBELL, Andrew , BOGER, Veronica
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1013
摘要： A method for isolating RNA from a biological sample, which comprises: (i) treating the sample with a lysis solution comprising a detergent at a pH of greater than 6 to form a lysate comprising an RNA-containing liquid phase; (ii) contacting the lysate with a first solid phase bearing a positive charge in the presence of a salt so as to precipitate DNA and protein from the RNA- containing liquid phase onto the first solid phase; (iii) separating the RNA-containing liquid phase from the solid phase; (iv) contacting the RNA-containing liquid phase with a non-ionic solid phase in the presence of a lower alcohol comprising ethanol or propanol to precipitate RNA onto the non-ionic solid phase to form an RNA-containing solid phase; (v) separating the RNA-containing solid phase from the liquid phase; and (vi) isolating RNA therefrom.
摘要翻译：一种用于从生物样品中分离RNA的方法，其包括：（i）用pH大于6的包含洗涤剂的裂解溶液处理样品以形成包含含RNA液相的裂解物; （ii）在盐的存在下使裂解物与带有正电荷的第一固相接触，以将DNA和蛋白质从含RNA液相沉淀到第一固相上; （iii）将含RNA的液相与固相分离; （iv）在含有乙醇或丙醇的低级醇存在下将含RNA的液相与非离子固相接触以将RNA沉淀到非离子固相上以形成含RNA的固相; （v）从液相中分离含RNA固相; 和（vi）从其中分离RNA。

7. 发明公开

EP1633869A1 METHOD FOR SEQUENTIALLY ISOLATING DNA AND RNA FROM THE SAME NUCLEIC ACID-CONTAINING SAMPLE 审中-公开
标题翻译：方法DNA和RNA的顺序孤立于同一核酸样品含有
公开(公告)号：EP1633869A1
公开(公告)日：2006-03-15
申请号：EP04739565.2
申请日：2004-06-03
申请人： Qiagen AS , QIAGEN GmbH
发明人： REITAN, Evy, H. , DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C12N15/10
CPC分类号： C12N15/1003 , C12N15/1006
摘要： The present invention refers to a method for isolating nucleic acids from a nucleic acid-containing sample. More specifically, it relates to a method for sequentially isolating DNA and RNA from the same nucleic acid-containing sample. Furthermore, the present invention refers to kit for sequentially isolating DNA and RNA from the same nucleic acid-containing sample.

8. 发明公开

EP1492804A1 PROCESS FOR ISOLATING NUCLEIC ACID WITH CHAOTROPE AGENTS AND AMMONIUM COMPOUNDS 有权
标题翻译：一种获得NUCLEIC使用离液和AMMONIUMREAGENTIEN
公开(公告)号：EP1492804A1
公开(公告)日：2005-01-05
申请号：EP03712478.1
申请日：2003-04-02
申请人： Qiagen AS
发明人： REITAN, Evy , DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C07H21/00 , G01N33/553 , C01B21/068 , C12N1/08 , C12N15/10 , C12Q1/70 , C12Q1/68
CPC分类号： C12N15/1006
摘要： A process for isolating nucleic acid from a nucleic acid-containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) providing a source of NH4+ or NH3; (d) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope and the NH4+ or NH3; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase.

9. 发明授权

EP1492804B1 PROCESS FOR ISOLATING NUCLEIC ACID WITH CHAOTROPE AGENTS AND AMMONIUM COMPOUNDS 有权
标题翻译：方法REINUNG使用离液和AMMONIUMREAGENTIEN核酸
公开(公告)号：EP1492804B1
公开(公告)日：2005-12-28
申请号：EP03712478.1
申请日：2003-04-02
申请人： Qiagen AS
发明人： REITAN, Evy , DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C07H21/00 , G01N33/553 , C01B21/068 , C12N1/08 , C12N15/10 , C12Q1/70 , C12Q1/68
CPC分类号： C12N15/1006
摘要： A process for isolating nucleic acid from a nucleic acid-containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) providing a source of NH4+ or NH3; (d) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope and the NH4+ or NH3; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase.

10. 发明授权

EP1492804B2 PROCESS FOR ISOLATING NUCLEIC ACID WITH CHAOTROPE AGENTS AND AMMONIUM COMPOUNDS 有权
标题翻译：方法REINUNG使用离液和AMMONIUMREAGENTIEN核酸
公开(公告)号：EP1492804B2
公开(公告)日：2016-03-09
申请号：EP03712478.1
申请日：2003-04-02
申请人： Qiagen AS
发明人： REITAN, Evy , DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C07H21/00 , G01N33/553 , C01B21/068 , C12N1/08 , C12N15/10 , C12Q1/70 , C12Q1/68
CPC分类号： C12N15/1006

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