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1. 发明申请

WO2011143339A1 MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS 审中-公开
标题翻译：具有增强检测特性的突变型PROTEASE生物传感器
公开(公告)号：WO2011143339A1
公开(公告)日：2011-11-17
申请号：PCT/US2011/036110
申请日：2011-05-11
申请人： PROMEGA CORPORATION , BINKOWSKI, Brock , BUTLER, Braeden , ENCELL, Lance, P. , FAN, Frank , HOOK, Brad , OTTO, Paul , VIDUGIRIS, Gediminas , WIGDAL, Susan , ZIMMERMAN, Kristopher
发明人： BINKOWSKI, Brock , BUTLER, Braeden , ENCELL, Lance, P. , FAN, Frank , HOOK, Brad , OTTO, Paul , VIDUGIRIS, Gediminas , WIGDAL, Susan , ZIMMERMAN, Kristopher
IPC分类号： C12N9/02 , C12Q1/66 , A01K67/027 , C12N5/00 , C12N15/00
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly- permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
摘要翻译：编码生物传感器多肽的多核苷酸，其包含修饰的环状置换的热稳定性萤光素酶和将热稳定性荧光素酶的C末端部分连接到热稳定性荧光素酶的N末端部分的接头。相对于亲本循环置换的热稳定性荧光素酶，修饰的环状置换的热稳定性荧光素酶被修饰。接头包含能够与细胞中的靶分子相互作用的传感器区域。在目标分子存在下，经修饰的环状置换的热稳定荧光素酶在生物传感器与靶分子相对于亲本循环置换的热稳定荧光素酶相互作用后具有增强的响应。或者，修饰的环状置换的热稳定荧光素酶在生物传感器与目标分子相互作用后相对于在不存在目标分子的情况下相对于经修饰的环状置换的热稳定性荧光素酶具有增强的响应。

2. 发明申请

WO2004042003A2 CELL LYSIS COMPOSITIONS, METHODS OF USE, APPARATUS, AND KIT 审中-公开
标题翻译：细胞分析组合物，使用方法，装置和试剂盒
公开(公告)号：WO2004042003A2
公开(公告)日：2004-05-21
申请号：PCT/US2003/030463
申请日：2003-09-26
申请人： PROMEGA CORPORATION , ENGEL, Laurie , SHULTZ, John, W. , JOHNSON, Tonny, M. , ZIMMERMAN, Kristopher , BOZEK, Laura, L. , STEVENS, Judith, N.
发明人： ENGEL, Laurie , SHULTZ, John, W. , JOHNSON, Tonny, M. , ZIMMERMAN, Kristopher , BOZEK, Laura, L. , STEVENS, Judith, N.
IPC分类号： C12N
CPC分类号： B01D15/3804 , B01D15/361 , B01J20/28009 , C07K1/36 , C11D1/44 , C11D1/662 , C11D1/72 , C11D1/88 , C11D3/3719 , C11D3/381 , C11D3/38636 , C12N1/06
摘要： Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.
摘要翻译：细胞裂解组合物，使用用于从宿主细胞中提取和分离蛋白质和肽分子并用于检测蛋白质或肽的存在的组合物，试剂盒和装置从宿主细胞中提取和分离蛋白质和肽的方法。该组合物允许从宿主细胞中提取和分离蛋白质和肽，而不需要机械破坏并且有或没有从细胞培养基中分离细胞。该组合物包含至少一种表面活性剂，其疏水 - 亲油平衡值在约11至约16的范围内; 和至少一种细胞膜改变化合物。

3. 发明申请

WO2013071237A1 MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS 审中-公开
标题翻译：具有增强检测特性的突变型PROTEASE生物传感器
公开(公告)号：WO2013071237A1
公开(公告)日：2013-05-16
申请号：PCT/US2012/064675
申请日：2012-11-12
申请人： PROMEGA CORPORATION
发明人： BINKOWSKI, Brock , BUTLER, Braeden , ENCELL, Lance, P. , FAN, Frank , HOOK, Brad , OTTO, Paul , RISMA, Kimberly , VIDUGIRIS, Gediminas , WIGDAL, Susan , ZIMMERMAN, Kristopher
IPC分类号： C12Q1/37 , C12Q1/66
CPC分类号： C12Q1/66 , C12Q1/37
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly- permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly- permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
摘要翻译：编码生物传感器多肽的多核苷酸，其包含修饰的环状置换的热稳定性萤光素酶和将热稳定性荧光素酶的C末端部分连接到热稳定性荧光素酶的N末端部分的接头。修饰的环状置换的热稳定性荧光素酶相对于亲本循环的热稳定荧光素酶进行了修饰。接头包含能够与细胞中的靶分子相互作用的传感器区域。在目标分子存在下，经修饰的环状置换的热稳定荧光素酶在生物传感器与靶分子相对于亲本循环置换的热稳定荧光素酶相互作用后具有增强的响应。或者，修饰的环状置换的热稳定荧光素酶在生物传感器与目标分子相互作用后相对于在不存在目标分子的情况下相对于经修饰的环状置换的热稳定性荧光素酶具有增强的响应。

4. 发明申请

WO2010127368A1 SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT 审中-公开
标题翻译：具有增强光输出功能的合成眼科植入物
公开(公告)号：WO2010127368A1
公开(公告)日：2010-11-04
申请号：PCT/US2010/033449
申请日：2010-05-03
申请人： PROMEGA CORPORATION , ENCELL, Lance, P. , WOOD, Keith, V. , WOOD, Monika, G. , HALL, Mary , OTTO, Paul , VIDUGIRIS, Gediminas , ZIMMERMAN, Kristopher
发明人： ENCELL, Lance, P. , WOOD, Keith, V. , WOOD, Monika, G. , HALL, Mary , OTTO, Paul , VIDUGIRIS, Gediminas , ZIMMERMAN, Kristopher
IPC分类号： C12N9/02 , C12Q1/66
CPC分类号： C12Q1/66 , C12N9/0004
摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
摘要翻译：编码经修饰的萤光素酶多肽的多核苷酸。修饰的荧光素酶多肽与野生型Oplophorus萤光素酶具有至少60％的氨基酸序列同一性，并且包括在对应于SEQ ID NO：1的野生型Oplophorus荧光素酶中的氨基酸的位置处的至少一个氨基酸取代。修饰的荧光素酶多肽相对于野生型Oplophorus萤光素酶具有增强的发光，增强的信号稳定性和增强的蛋白质稳定性中的至少一种。

5. 发明公开

EP2776573A1 MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS 审中-公开
标题翻译： MUTANTE PROTEASE-BIOSENSOREN MIT VERBESSERTEN ERKENNUNGSEIGENSCHAFTEN
公开(公告)号：EP2776573A1
公开(公告)日：2014-09-17
申请号：EP12791055.2
申请日：2012-11-12
申请人： Promega Corporation
发明人： BINKOWSKI, Brock , BUTLER, Braeden , ENCELL, Lance, P. , FAN, Frank , HOOK, Brad , OTTO, Paul , RISMA, Kimberly , VIDUGIRIS, Gediminas , WIGDAL, Susan , ZIMMERMAN, Kristopher
IPC分类号： C12Q1/37 , C12Q1/66
CPC分类号： C12Q1/66 , C12Q1/37
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly- permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly- permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
摘要翻译：编码生物传感器多肽的多核苷酸，其包含修饰的环状置换的热稳定性萤光素酶和将热稳定性萤光素酶的C末端部分连接到热稳定性萤光素酶的N末端部分的接头。相对于亲本循环置换的热稳定性荧光素酶，修饰的环状置换的热稳定性荧光素酶被修饰。接头包含能够与细胞中的靶分子相互作用的传感器区域。修饰的环状置换的热稳定性荧光素酶在目标分子存在下相对于亲本循环置换的热稳定性荧光素酶在生物传感器与靶分子相互作用后具有增强的响应。或者，修饰的环状置换的热稳定荧光素酶在生物传感器与目标分子相互作用后相对于在不存在靶分子的情况下相对于经修饰的环状置换的热稳定性荧光素酶具有增强的响应。

6. 发明公开

EP1558748A2 CELL LYSIS COMPOSITIONS, METHODS OF USE, APPARATUS, AND KIT 审中-公开
标题翻译： ZELLYSEZUSAMMENSETZUNGEN，使用方法，设备和试剂盒
公开(公告)号：EP1558748A2
公开(公告)日：2005-08-03
申请号：EP03796345.1
申请日：2003-09-26
申请人： PROMEGA CORPORATION
发明人： ENGEL, Laurie , SHULTZ, John, W. , JOHNSON, Tonny, M. , ZIMMERMAN, Kristopher , BOZEK, Laura, L. , STEVENS, Judith, N.
IPC分类号： C12P21/06 , C07H19/00 , C07H21/00
CPC分类号： B01D15/3804 , B01D15/361 , B01J20/28009 , C07K1/36 , C11D1/44 , C11D1/662 , C11D1/72 , C11D1/88 , C11D3/3719 , C11D3/381 , C11D3/38636 , C12N1/06
摘要： Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.

7. 发明授权

EP2456864B1 SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT 有权
标题翻译：与增加的光功率合成OPLOPHORUS，荧光素酶
公开(公告)号：EP2456864B1
公开(公告)日：2015-11-11
申请号：EP10717013.6
申请日：2010-05-03
申请人： Promega Corporation
发明人： ENCELL, Lance, P. , WOOD, Keith, V. , WOOD, Monika, G. , HALL, Mary , OTTO, Paul , VIDUGIRIS, Gediminas , ZIMMERMAN, Kristopher
IPC分类号： C12N9/02 , C12Q1/66
CPC分类号： C12Q1/66 , C12N9/0004
摘要： The present invention discloses a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

8. 发明授权

EP2569423B1 MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS 有权
标题翻译：具有改进的侦测功能突变体PROTHEASE生物传感器
公开(公告)号：EP2569423B1
公开(公告)日：2015-12-02
申请号：EP11720279.6
申请日：2011-05-11
申请人： Promega Corporation
发明人： BINKOWSKI, Brock , BUTLER, Braeden , ENCELL, Lance, P. , FAN, Frank , HOOK, Brad , OTTO, Paul , VIDUGIRIS, Gediminas , WIGDAL, Susan , ZIMMERMAN, Kristopher
IPC分类号： C12Q1/37 , C12Q1/66 , C12N9/02 , A01K67/027 , A61K49/00
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007

9. 发明公开

EP2569423A1 MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS 有权
标题翻译：具有改进的侦测功能突变体PROTHEASE生物传感器
公开(公告)号：EP2569423A1
公开(公告)日：2013-03-20
申请号：EP11720279.6
申请日：2011-05-11
申请人： Promega Corporation
发明人： BINKOWSKI, Brock , BUTLER, Braeden , ENCELL, Lance, P. , FAN, Frank , HOOK, Brad , OTTO, Paul , VIDUGIRIS, Gediminas , WIGDAL, Susan , ZIMMERMAN, Kristopher
IPC分类号： C12N9/02 , C12Q1/66 , A01K67/027 , C12N5/00 , C12N15/00
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly- permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

10. 发明公开

EP2456864A1 SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT 有权
标题翻译：与增加的光功率合成OPLOPHORUS，荧光素酶
公开(公告)号：EP2456864A1
公开(公告)日：2012-05-30
申请号：EP10717013.6
申请日：2010-05-03
申请人： Promega Corporation
发明人： ENCELL, Lance, P. , WOOD, Keith, V. , WOOD, Monika, G. , HALL, Mary , OTTO, Paul , VIDUGIRIS, Gediminas , ZIMMERMAN, Kristopher
IPC分类号： C12N9/02 , C12Q1/66
CPC分类号： C12Q1/66 , C12N9/0004
摘要： The present invention discloses a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

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