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1. 发明申请

US20160115522A1 MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS 有权
标题翻译：具有增强检测特性的突变型PROTEASE生物传感器
公开(公告)号：US20160115522A1
公开(公告)日：2016-04-28
申请号：US14990279
申请日：2016-01-07
申请人： Promega Corporation
发明人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC分类号： C12Q1/66 , A61K49/00 , C12N9/02
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
摘要翻译：编码包含修饰的环状置换的热稳定性荧光素酶的生物传感器多肽的多核苷酸和将热稳定性荧光素酶的C末端部分连接到热稳定性萤光素酶的N末端部分的接头。相对于亲本循环置换的热稳定性荧光素酶，修饰的环状置换的热稳定性荧光素酶被修饰。接头包含能够与细胞中的靶分子相互作用的传感器区域。经修饰的环状置换的热稳定荧光素酶在目标分子存在下相对于亲本循环置换的热稳定性荧光素酶在生物传感器与靶分子相互作用后具有增强的响应。或者，修饰的环状置换的热稳定荧光素酶在生物传感器与目标分子相互作用后相对于在不存在目标分子的情况下相对于经修饰的环状置换的热稳定性荧光素酶具有增强的响应。

2. 发明授权

US09757478B2 Mutant protease biosensors with enhanced detection characteristics 有权
公开(公告)号：US09757478B2
公开(公告)日：2017-09-12
申请号：US14990279
申请日：2016-01-07
申请人： Promega Corporation
发明人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC分类号： A61K49/00 , C12N9/02 , C12Q1/37 , C12Q1/66 , A01K67/027
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

3. 发明申请

US20140273156A1 LUCIFERASE BIOSENSOR 审中-公开
标题翻译： LUCIFERASE生物传感器
公开(公告)号：US20140273156A1
公开(公告)日：2014-09-18
申请号：US14180451
申请日：2014-02-14
申请人： PROMEGA CORPORATION
发明人： Frank Fan , Martin Ken Lewis , John W. Shultz , Keith V. Wood , Braeden Butler
IPC分类号： C12N9/02
CPC分类号： C12N9/0069 , C07K2319/20 , C07K2319/21 , C07K2319/23 , C12Q1/66 , G01N2500/04
摘要： A modified beetle luciferase protein which is an environmentally sensitive reporter protein is provided.
摘要翻译：提供了一种环境敏感的报告蛋白的改良甲壳质萤光素酶蛋白。

4. 发明申请

US20160169869A1 SYSTEMS AND METHODS FOR ASSESSING MODULATORS OF IMMUNE CHECKPOINTS 审中-公开
标题翻译：用于评估免疫调节剂调节剂的系统和方法
公开(公告)号：US20160169869A1
公开(公告)日：2016-06-16
申请号：US14947660
申请日：2015-11-20
申请人： Promega Corporation
发明人： Mei Cong , Zhijie Jey Cheng , Natasha Karassina , Jamison Grailer , Frank Fan
IPC分类号： G01N33/50 , C07K16/28 , C12Q1/68 , C07K14/705
CPC分类号： G01N33/5041 , A61K35/17 , A61K38/1774 , C07K14/70532 , C07K16/2803 , C07K16/2809 , C07K16/2818 , C07K2317/75 , C07K2317/76 , C12Q1/6897 , G01N33/5044 , G01N2333/7051 , G01N2333/70532
摘要： Provided herein are compositions, systems, and methods for assessing modulators of immune checkpoints. In particular, artificial antigen presenting cells (aAPCs) and immune effector cells are provided to assess the potency of test agents to inhibit immune checkpoints.
摘要翻译：本文提供了用于评估免疫检查点调节剂的组合物，系统和方法。特别地，提供人造抗原呈递细胞（aAPCs）和免疫效应细胞以评估测试试剂抑制免疫检查点的效力。

5. 发明授权

US09339561B2 Mutant protease biosensors with enhanced detection characteristics 有权
标题翻译：具有增强检测特性的突变蛋白酶生物传感器
公开(公告)号：US09339561B2
公开(公告)日：2016-05-17
申请号：US14269669
申请日：2014-05-05
申请人： Promega Corporation
发明人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC分类号： A61K49/00 , C12N9/02 , C12Q1/37 , C12Q1/66 , A01K67/027
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
摘要翻译：编码包含修饰的环状置换的热稳定性荧光素酶的生物传感器多肽的多核苷酸和将热稳定性荧光素酶的C末端部分连接到热稳定性萤光素酶的N末端部分的接头。相对于亲本循环置换的热稳定性荧光素酶，修饰的环状置换的热稳定性荧光素酶被修饰。接头包含能够与细胞中的靶分子相互作用的传感器区域。经修饰的环状置换的热稳定荧光素酶在目标分子存在下相对于亲本循环置换的热稳定性荧光素酶在生物传感器与靶分子相互作用后具有增强的响应。或者，修饰的环状置换的热稳定荧光素酶在生物传感器与目标分子相互作用后相对于在不存在目标分子的情况下相对于经修饰的环状置换的热稳定性荧光素酶具有增强的响应。

6. 发明授权

US09290794B2 Mutant protease biosensors with enhanced detection characteristics 有权
公开(公告)号：US09290794B2
公开(公告)日：2016-03-22
申请号：US13674655
申请日：2012-11-12
申请人： PROMEGA CORPORATION
发明人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Kimberly Risma , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC分类号： C12Q1/66 , C12Q1/37 , C12N9/02
CPC分类号： C12Q1/66 , C12N9/0069 , C12Q1/37
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

7. 发明授权

US09290745B2 Luciferase biosensor 有权
标题翻译：萤光素酶生物传感器
公开(公告)号：US09290745B2
公开(公告)日：2016-03-22
申请号：US14180451
申请日：2014-02-14
申请人： PROMEGA CORPORATION
发明人： Frank Fan , Martin Ken Lewis , John W. Shultz , Keith V. Wood , Braeden Butler
IPC分类号： C12N15/12 , C12N9/02 , C12Q1/66
CPC分类号： C12N9/0069 , C07K2319/20 , C07K2319/21 , C07K2319/23 , C12Q1/66 , G01N2500/04
摘要： A modified beetle luciferase protein which is an environmentally sensitive reporter protein is provided.
摘要翻译：提供了一种环境敏感的报告蛋白的改良甲壳质萤光素酶蛋白。

8. 发明授权

US10077433B2 Permuted and nonpermuted luciferase biosensors 有权
公开(公告)号：US10077433B2
公开(公告)日：2018-09-18
申请号：US15168631
申请日：2016-05-31
申请人： PROMEGA CORPORATION
发明人： Brock Binkowski , Frank Fan , Susan Wigdal , Keith V. Wood , Monika G. Wood
IPC分类号： C12N9/02 , G01N33/84 , C12Q1/66
CPC分类号： C12N9/0069 , C12N9/0004 , C12Q1/66 , C12Y113/12007 , G01N33/84
摘要： A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.

9. 发明申请

US20160340654A1 PERMUTED AND NONPERMUTED LUCIFERASE BIOSENSORS 审中-公开
标题翻译：未经授权的和未经授权的LUCIFERASE生物传感器
公开(公告)号：US20160340654A1
公开(公告)日：2016-11-24
申请号：US15168631
申请日：2016-05-31
申请人： PROMEGA CORPORATION
发明人： Brock Binkowski , Frank Fan , Susan Wigdal , Keith V. Wood , Monika G. Wood
IPC分类号： C12N9/02 , G01N33/84 , C12Q1/66
CPC分类号： C12N9/0069 , C12N9/0004 , C12Q1/66 , C12Y113/12007 , G01N33/84
摘要： A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.
摘要翻译：提供了一种修饰的荧光素酶蛋白，其是用于分子的传感器，包括cAMP，cGMP，钙，其螯合剂，激酶或磷酸酶。还提供了循环置换的anthozoan萤光素酶蛋白质和十足配甲壳素荧光素酶蛋白质，任选地含有一个或多个异源氨基酸序列，其中至少一个直接或间接与感兴趣的分子相互作用。进一步提供了一种修饰的anthozoan萤光素酶蛋白质和十足类甲壳类荧光素酶蛋白质，其含有一个或多个异源氨基酸序列的插入，其中至少一个与感兴趣的分子直接或间接相互作用。

10. 发明授权

US09248201B2 Mutant protease biosensors with enhanced detection characteristics 有权
公开(公告)号：US09248201B2
公开(公告)日：2016-02-02
申请号：US14269689
申请日：2014-05-05
申请人： Promega Corporation
发明人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC分类号： C12N9/02 , C12Q1/37 , C12Q1/66 , A61K49/00 , A01K67/027
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

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