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    • 6. 发明授权
    • Isozyme of autoclavable superoxide dismutase (SOD), a process for the identification and extraction of the SOD and use of the said SOD in cosmetic, food, and pharmaceutical compositions
    • 高压灭菌超氧化物歧化酶(SOD)的同功酶,用于鉴定和提取SOD的过程以及所述SOD在化妆品,食品和药物组合物中的用途
    • US07037697B2
    • 2006-05-02
    • US10274053
    • 2002-10-21
    • Sanjay KumarRashmita SahooParamvir Singh Ahuja
    • Sanjay KumarRashmita SahooParamvir Singh Ahuja
    • C12N9/02C12N9/00A61K33/44
    • A23G4/123A61K8/66A61K38/00A61Q19/00C12N9/0089C12Y115/01001Y10S977/915Y10S977/926
    • The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plant Potentilla atrosanguinea Lodd. Var. areyrophylla, said isozyme having the following characteristics, O2− scavenging activity remains same before and after autoclaving; scavenges O2− from sub-zero temperature of −20° C. to high temperature of +80° C.; O2− scavenging activity at 25° C. for 30 days without adding any stabilizing agent such as polyols or sugars; O2− scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4° C. for at least 8 months; contamination free and infection free from any living micro- and/or macro-organism after autoclaving; possesses temperature optima at 0° C.; possesses a molecular weight of 33 kD under non-denaturating conditions; possesses a molecular weight of 36 kD under denaturating conditions; has clear peaks in UV range at 268 and 275 nm; has an enzyme turnover number of 19.53×104% per nmol per min at 0° C.; and requires Cu/Zn as a co-factor, method for the preparation of the purified isozyme of autoclavable superoxide dismutase and formulations containing the said autoclavable superoxide dismutase.
    • 本发明涉及从植物Potentilla atrosanguinea Lodd提取的可高压灭菌的超氧化物歧化酶的新型纯化同功酶。 可变 所述同功酶具有以下特征,即高压灭菌之前和之后的清除活性相同。 从-20°C的零度以上至+ 80°C的高温,清除O 2 - 。 O 2清除活性在25℃下30天,而不添加任何稳定剂如多元醇或糖; 在盐水(0.9%氯化钠)存在下,向对照(不含0.9%氯化钠)的61.8%清除活性,在4℃下稳定。 至少8个月; 高压灭菌后无任何生物微生物和/或大生物感染的免污染和感染; 在0°C时具有最佳温度。 在非变性条件下具有33 kD的分子量; 在变性条件下具有36kD的分子量; 在268和275nm的UV范围内具有清晰的峰; 在0℃下每分钟酶转化数为19.53×10 4%/ nmol /分钟。 并且需要Cu / Zn作为辅助因子,制备可高压灭菌的超氧化物歧化酶的纯化同功酶的方法和含有所述高压灭菌的超氧化物歧化酶的制剂。