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1. 发明申请

WO2013070854A1 METHOD FOR DISPENSING GRAINS OF POLLEN 审中-公开
标题翻译：分配花粉颗粒的方法
公开(公告)号：WO2013070854A1
公开(公告)日：2013-05-16
申请号：PCT/US2012/064068
申请日：2012-11-08
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： COPE, Jason M. , KRONE, Todd L. , MCAVOY, Bradley T.
IPC分类号： A01H1/02
CPC分类号： A01G7/00 , A01H1/02
摘要： Methods for dispensing pollen contained in a solution onto a plant for pollination are provided herein. In one example embodiment, a method for dispensing grains of pollen comprises introducing a plurality of grains of pollen into a solution to create a pollen solution mixture. In some embodiments, the pollen solution mixture is contained in a pollen storage container. The method further comprises connecting the pollen storage container containing the pollen solution mixture to a dispensing device. The method further includes propelling at least a portion of the pollen solution mixture from the pollen storage container using the dispensing device to pollinate a plant.
摘要翻译：本发明提供将溶液中所含的花粉分配到植物上进行授粉的方法。在一个示例性实施例中，一种用于分配花粉颗粒的方法包括将多个花粉颗粒引入溶液中以产生花粉溶液混合物。在一些实施方案中，将花粉溶液混合物包含在花粉储存容器中。该方法还包括将含有花粉溶液混合物的花粉储存容器连接到分配装置。该方法还包括使用分配装置从花粉储存容器推进至少一部分花粉溶液混合物以授粉植物。

2. 发明申请

WO2012129200A3 APPARATUS AND METHOD FOR DISPENSING GRAINS OF POLLEN 审中-公开
公开(公告)号：WO2012129200A3
公开(公告)日：2012-09-27
申请号：PCT/US2012/029743
申请日：2012-03-20
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , COPE, Jason M. , MCAVOY, Bradley T.
发明人： COPE, Jason M. , MCAVOY, Bradley T.
IPC分类号： A01H1/02 , A01H1/00
摘要： An apparatus configured for dispensing grains of pollen may include a pollen storage container, a flow source configured to dispense the grains of pollen from the pollen storage container, and a control mechanism configured to selectively activate the flow source. The apparatus may or may not apply an electrical charge to the grains of pollen. Related methods may include steps of collecting pollen, inserting the pollen into a pollen storage container, connecting the pollen storage container to a dispensing device such as the above-described apparatus, and dispensing the pollen from the pollen storage container with the dispensing device. Known grains of pollen may be dispensed to create predetermined gamete crosses with known plants. Further, an outlet of a fan which dispenses the pollen from the pollen storage container or other parts of the dispensing device may be cleaned when swapping the pollen storage container to prevent cross-contamination.

3. 发明申请

WO2013070846A1 LARGE SCALE METHOD FOR DISPENSING GRAINS OF POLLEN 审中-公开
标题翻译：用于分配POLLAIN颗粒的大规模方法
公开(公告)号：WO2013070846A1
公开(公告)日：2013-05-16
申请号：PCT/US2012/064059
申请日：2012-11-08
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： COPE, Jason M. , KRONE, Todd L.
IPC分类号： A01H1/02
CPC分类号： A01H1/025
摘要： A method is provided for bulk pollination of a plurality of plants. In various embodiments, the method includes collecting a plurality of known grains of pollen, loading the plurality of known grains of pollen into a bulk pollen distributing apparatus, sensing a target pollination location of each of the plurality of plants using an electronic sensor, and distributing the known grains of pollen to the plurality of plants with the bulk pollen distributing apparatus when the electronic sensor senses the target pollination location of each of the plants within a predefined proximity, thereby pollinating the plurality of plants. A plurality of plants may be prepared for controlled pollination by preventing pollination of the plurality of plants by an alternate pollen source. Known grains of pollen may be dispensed to create predetermined gamete crosses with known plants.
摘要翻译：提供了用于多个植物的大量授粉的方法。在各种实施方案中，该方法包括收集多个已知的花粉颗粒，将多个已知的花粉颗粒加载到大块花粉分配装置中，使用电子传感器感测多个植物中的每个植物的目标授粉位置，并且分布当电子传感器在预定义的接近度内感测到每个植物的目标授粉位置，从而授粉多个植物时，将具有散装花粉分配装置的已知花粉粒数分配给多个植物。可以通过防止由替代花粉源授粉多个植物来制备多个植物用于受控授粉。可以分配已知的花粉粒以产生与已知植物的预定的配子交叉。

4. 发明申请

WO2012170651A2 APPARATUS, SYSTEM, AND METHOD FOR AUTOMATED DISPENSING OF A SUBSTANCE ON ONE OR MORE SEEDS 审中-公开
标题翻译：用于自动分配一种或多种种子的物质的装置，系统和方法
公开(公告)号：WO2012170651A2
公开(公告)日：2012-12-13
申请号：PCT/US2012/041283
申请日：2012-06-07
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , BLAHNIK, Amy D. , COPE, Jason M. , MCAVOY, Bradley T. , WAN, Gang
发明人： BLAHNIK, Amy D. , COPE, Jason M. , MCAVOY, Bradley T. , WAN, Gang
IPC分类号： A01C7/08 , A01C7/00
CPC分类号： A01C1/06
摘要： Embodiments of the present invention provide automated apparatus, systems, and methods for dispensing a substance on a seed. An example embodiment provides a system for automated dispensing of a substance on a seed. The system comprises a seed container with a plurality of compartments each configured to retain a seed. The system further comprises a supply tank configured to retain the substance. The system also comprises at least one dispensing tip configured to dispense the substance and a pump connected to the supply tank and to the dispensing tip. The pump is configured to pump the substance from the supply tank to the dispensing tip. The system further comprises a controller configured to move at least one of the seed container or the dispensing tip relative to the other and to direct the pump to pump the substance into a compartment of the seed container.
摘要翻译：本发明的实施例提供了用于在种子上分配物质的自动化装置，系统和方法。一个示例性实施例提供了一种用于在种子上自动分配物质的系统。该系统包括具有多个隔室的种子容器，每个隔间被配置为保持种子。该系统还包括配置成保持物质的供应罐。该系统还包括构造成分配物质的至少一个分配尖端和连接到供应罐和分配尖端的泵。泵被配置为将物质从供应罐泵送到分配尖端。该系统还包括控制器，该控制器被配置为相对于另一种移动种子容器或分配尖端中的至少一个并且引导泵将物质泵送到种子容器的隔室中。

5. 发明申请

WO2012129199A2 APPARATUSES AND METHODS FOR EVALUATING AND SORTING POLLEN AND PLANTS 审中-公开
标题翻译：用于评估和分类POLLEN和植物的装置和方法
公开(公告)号：WO2012129199A2
公开(公告)日：2012-09-27
申请号：PCT/US2012/029739
申请日：2012-03-20
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , COPE, Jason M.
发明人： COPE, Jason M.
IPC分类号： A01H1/04 , A01H1/00
CPC分类号： A01H1/04 , A01H1/02 , C12Q1/6895 , G01N33/56961
摘要： A method for sorting pollen containing genetic elements of interest may include associating a genetic marker with seeds defining the genetic elements of interest, growing plants from the seeds, collecting pollen from the seeds, evaluating the pollen for presence of the genetic markers, and sorting the pollen based on presence or absence of the genetic markers. A method of determining viability of pollen may evaluate the optical density of grains of pollen and compare the optical density to an optical density threshold. Further, a method for identifying plants defining a genetic element of interest may include associating a genetic marker with seeds defining the genetic element of interest, growing plants from the seeds, and evaluating the plants for presence of the genetic marker. Additionally, a method for identifying a genotype of pollen may include evaluating pollen via spectral and/or hyperspectral imaging, and comparing the wavelength pattern to known wavelength patterns.
摘要翻译：用于分选含有感兴趣的遗传元件的花粉的方法可以包括将遗传标记物与限定感兴趣的遗传元件的种子，从种子生长的植物，从种子收集花粉，评估花粉的遗传标记的存在，以及对基于存在或不存在遗传标记的花粉。确定花粉生存力的方法可以评估花粉颗粒的光密度，并将光密度与光密度阈值进行比较。此外，用于鉴定限定感兴趣的遗传元件的植物的方法可以包括将遗传标记与定义感兴趣的遗传元件的种子，从种子生长植物，以及评估植物的遗传标记的存在。此外，用于鉴定花粉基因型的方法可以包括通过光谱和/或高光谱成像评估花粉，并将波长图案与已知波长图案进行比较。

6. 发明公开

EP2688394A2 APPARATUS AND METHOD FOR DISPENSING GRAINS OF POLLEN 审中-公开
标题翻译：用于分配花粉粒的装置和方法
公开(公告)号：EP2688394A2
公开(公告)日：2014-01-29
申请号：EP12760235.7
申请日：2012-03-20
申请人： Pioneer Hi-Bred International, Inc.
发明人： COPE, Jason M. , MCAVOY, Bradley T.
IPC分类号： A01H1/02 , A01H1/00
CPC分类号： A01H1/025 , A01H1/02
摘要： An apparatus configured for dispensing grains of pollen may include a pollen storage container, a flow source configured to dispense the grains of pollen from the pollen storage container, and a control mechanism configured to selectively activate the flow source. The apparatus may or may not apply an electrical charge to the grains of pollen. Related methods may include steps of collecting pollen, inserting the pollen into a pollen storage container, connecting the pollen storage container to a dispensing device such as the above-described apparatus, and dispensing the pollen from the pollen storage container with the dispensing device. Known grains of pollen may be dispensed to create predetermined gamete crosses with known plants. Further, an outlet of a fan which dispenses the pollen from the pollen storage container or other parts of the dispensing device may be cleaned when swapping the pollen storage container to prevent cross-contamination.
摘要翻译：配置用于分配花粉粒的设备可以包括花粉存储容器，配置成从花粉存储容器分配花粉粒的流源和配置为选择性地启动流源的控制机构。该装置可能会或可能不会对花粉粒施加电荷。相关方法可以包括收集花粉，将花粉插入花粉储存容器，将花粉储存容器连接至诸如上述装置的分配装置，并且将花粉储存容器中的花粉与分配装置分配的步骤。可以分配已知的花粉粒以与已知植物产生预定的配子杂交。此外，当交换花粉储存容器以防止交叉污染时，可以清洁从花粉储存容器或分配装置的其他部分分配花粉的风扇的出口。

7. 发明公开

EP2688395A2 APPARATUSES AND METHODS FOR EVALUATING AND SORTING POLLEN AND PLANTS 审中-公开
标题翻译：器件和方法进行评价和分级花粉和植物
公开(公告)号：EP2688395A2
公开(公告)日：2014-01-29
申请号：EP12760752.1
申请日：2012-03-20
申请人： Pioneer Hi-Bred International, Inc.
发明人： COPE, Jason M.
IPC分类号： A01H1/04 , A01H1/00
CPC分类号： A01H1/04 , A01H1/02 , C12Q1/6895 , G01N33/56961
摘要： A method for sorting pollen containing genetic elements of interest may include associating a genetic marker with seeds defining the genetic elements of interest, growing plants from the seeds, collecting pollen from the seeds, evaluating the pollen for presence of the genetic markers, and sorting the pollen based on presence or absence of the genetic markers. A method of determining viability of pollen may evaluate the optical density of grains of pollen and compare the optical density to an optical density threshold. Further, a method for identifying plants defining a genetic element of interest may include associating a genetic marker with seeds defining the genetic element of interest, growing plants from the seeds, and evaluating the plants for presence of the genetic marker. Additionally, a method for identifying a genotype of pollen may include evaluating pollen via spectral and/or hyperspectral imaging, and comparing the wavelength pattern to known wavelength patterns.

8. 发明公开

EP2775823A1 METHOD FOR DISPENSING GRAINS OF POLLEN 审中-公开
标题翻译：程序刊发花粉粒
公开(公告)号：EP2775823A1
公开(公告)日：2014-09-17
申请号：EP12787327.1
申请日：2012-11-08
申请人： Pioneer Hi-Bred International, Inc.
发明人： COPE, Jason M. , KRONE, Todd L. , MCAVOY, Bradley T.
IPC分类号： A01H1/02
CPC分类号： A01G7/00 , A01H1/02
摘要： Methods for dispensing pollen contained in a solution onto a plant for pollination are provided herein. In one example embodiment, a method for dispensing grains of pollen comprises introducing a plurality of grains of pollen into a solution to create a pollen solution mixture. In some embodiments, the pollen solution mixture is contained in a pollen storage container. The method further comprises connecting the pollen storage container containing the pollen solution mixture to a dispensing device. The method further includes propelling at least a portion of the pollen solution mixture from the pollen storage container using the dispensing device to pollinate a plant.

9. 发明公开

EP2775822A1 LARGE SCALE METHOD FOR DISPENSING GRAINS OF POLLEN 审中-公开
标题翻译：方法花粉粒MASS发行
公开(公告)号：EP2775822A1
公开(公告)日：2014-09-17
申请号：EP12787326.3
申请日：2012-11-08
申请人： Pioneer Hi-Bred International, Inc.
发明人： COPE, Jason M. , KRONE, Todd L.
IPC分类号： A01H1/02
CPC分类号： A01H1/025
摘要： A method is provided for bulk pollination of a plurality of plants. In various embodiments, the method includes collecting a plurality of known grains of pollen, loading the plurality of known grains of pollen into a bulk pollen distributing apparatus, sensing a target pollination location of each of the plurality of plants using an electronic sensor, and distributing the known grains of pollen to the plurality of plants with the bulk pollen distributing apparatus when the electronic sensor senses the target pollination location of each of the plants within a predefined proximity, thereby pollinating the plurality of plants. A plurality of plants may be prepared for controlled pollination by preventing pollination of the plurality of plants by an alternate pollen source. Known grains of pollen may be dispensed to create predetermined gamete crosses with known plants.

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