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1. 发明申请

US20080155705A1 Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms 有权
公开(公告)号：US20080155705A1
公开(公告)日：2008-06-26
申请号：US10566944
申请日：2004-07-16
申请人： Thorsten Zank , Jorg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
发明人： Thorsten Zank , Jorg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
IPC分类号： A01K67/00 , C12N15/82 , C07C51/00 , C12N15/11 , A01H5/00 , C12N15/00 , C12N1/20
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

2. 发明申请

US20070224661A1 Method for Producing Unsaturated Omega-3-Fatty Acids in Transgenic Organisms 有权
标题翻译：在转基因生物中生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US20070224661A1
公开(公告)日：2007-09-27
申请号：US10590958
申请日：2005-02-23
申请人： Petra Cirpus , Jorg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jorg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： C12P1/04
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to a process for production of unsaturated ω-3-fatty acids and to a process for production of triglycerides with an elevated content of unsaturated fatty acids, especially of ω-3-fatty acids having more than three double bonds. The invention relates to the production of a transgenic organism, preferably of a transgenic plant or of a transgenic microorganism, with an elevated content of unsaturated ω-3-fatty acids, oils or lipids having ω-3-double bonds as the result of the expression of an ω-3-desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example of the genus and species Phytophtora infestans. The invention furthermore relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising at least one nucleic acid sequence according to the invention, at least one vector comprising the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention, and to their use. The invention moreover relates to unsaturated fatty acids and triglycerides with an elevated content of unsaturated fatty acids and their use.
摘要翻译：本发明涉及一种生产不饱和ω-3-脂肪酸的方法，以及一种具有升高的不饱和脂肪酸含量，特别是具有三个以上双键的ω-3-脂肪酸含量的甘油三酯的制备方法。本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有升高的不饱和ω-3-脂肪酸含量，油或具有ω-3-双键的脂质作为来自真菌的真菌的ω-3-去饱和酶的表达，例如疫霉属（Phytophtora），例如属和种类疫霉属（Stytophtora infestans）。本发明还涉及包含至少一个根据本发明的核酸序列的核酸序列，核酸构建体，载体和生物体，至少一种包含核酸序列和/或核酸构建体的载体，以及转基因生物包括上述核酸序列，核酸构建体和/或载体。本发明的另一部分涉及通过根据本发明的方法生产的油，脂和/或脂肪酸及其用途。本发明还涉及不饱和脂肪酸和不饱和脂肪酸含量升高的甘油三酸酯及其用途。

3. 发明授权

US08373024B2 Method for producing unsaturated ω-3-fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US08373024B2
公开(公告)日：2013-02-12
申请号：US12768227
申请日：2010-04-27
申请人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： A01H5/00 , C12N15/82 , C12N1/20 , C07H21/04
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to nucleic acid sequences coding for polypeptides with ω-3-desaturase activity. The invention furthermore relates to nucleic acid constructs, vectors and organisms comprising at least one nucleic acid sequence according to the invention, at least one vector comprising the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.
摘要翻译：本发明涉及编码具有ω-3-去饱和酶活性的多肽的核酸序列。本发明还涉及包含至少一个本发明核酸序列的至少一种核酸构建体，载体和生物体，至少一种包含核酸序列和/或核酸构建体的载体，转基因生物体包含上述核酸序列，核酸构建体和/或载体。

4. 发明申请

US20100212034A1 Method For Producing Unsaturated Omega-3-Fatty Acids In Transgenic Organisms 有权
标题翻译：在转基因生物中生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US20100212034A1
公开(公告)日：2010-08-19
申请号：US12768227
申请日：2010-04-27
申请人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： C07H21/04 , C12N15/63 , A01K67/027 , A01H5/00 , C12N1/00
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to nucleic acid sequences coding for polypeptides with ω-3-desaturase activity. The invention furthermore relates to nucleic acid constructs, vectors and organisms comprising at least one nucleic acid sequence according to the invention, at least one vector comprising the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.
摘要翻译：本发明涉及编码具有ω-3-去饱和酶活性的多肽的核酸序列。本发明还涉及包含至少一个本发明的核酸序列的核酸构建体，载体和生物体，至少一种包含核酸序列和/或核酸构建体的载体，转基因生物体包含上述核酸序列，核酸构建体和/或载体。

5. 发明授权

US09433228B2 Method for the production of multiple-unsaturated fatty acids in transgenic organisms 有权
公开(公告)号：US09433228B2
公开(公告)日：2016-09-06
申请号：US10566944
申请日：2004-07-16
申请人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
发明人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
IPC分类号： C12N15/82 , A23D9/00 , C12N9/02 , C12N9/10 , C12P7/64
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceæ such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

6. 发明授权

US07777098B2 Method for producing unsaturated ω-3-fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US07777098B2
公开(公告)日：2010-08-17
申请号：US10590958
申请日：2005-02-23
申请人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： C12N15/82 , C12N5/10 , C12N1/20
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to a process for production of unsaturated ω-3-fatty acids and to a process for production of triglycerides with an elevated content of unsaturated fatty acids, especially of ω-3-fatty acids having more than three double bonds. The invention relates to the production of a transgenic organism, preferably of a transgenic plant or of a transgenic microorganism, with an elevated content of unsaturated ω-3-fatty acids, oils or lipids having ω-3-double bonds as the result of the expression of an ω-3-desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example of the genus and species Phytophtora infestans.
摘要翻译：本发明涉及一种不饱和ω-3-脂肪酸的制备方法以及具有不饱和脂肪酸含量升高的甘油三酯的制备方法，特别是具有三个以上双键的ω-3-脂肪酸。本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有升高的不饱和ω-3-脂肪酸含量，油或具有ω-3-双键的脂质作为来自真菌的真菌的ω-3-去饱和酶的表达，例如疫霉属（Phytophtora），例如属和种类疫霉属（Pytophtora infestans）。

7. 发明申请

US20090083882A1 METHOD FOR INCREASING THE TOTAL OIL CONTENT IN OIL PLANTS 审中-公开
标题翻译：增加油田总油含量的方法
公开(公告)号：US20090083882A1
公开(公告)日：2009-03-26
申请号：US12092603
申请日：2006-11-06
申请人： Thorsten Zank , Oliver Oswald , Jorg Bauer , Helene Vigeolas , Peter Geigenberger , Peter Waldeck , Mark Stitt
发明人： Thorsten Zank , Oliver Oswald , Jorg Bauer , Helene Vigeolas , Peter Geigenberger , Peter Waldeck , Mark Stitt
IPC分类号： C12N15/82 , A01H1/04
CPC分类号： C12N15/8247 , C12N9/0006
摘要： The invention relates to methods of increasing the total oil content and/or the glycerol 3-phosphate content in transgenic oil crop plants which comprise at least 20% by weight of oleic acid based on the total fatty acid content, preferably in plant seeds, by expressing glycerol 3-phosphate dehydrogenases (G3PDHs) from yeasts, preferably from Saccharomyces cerevisiae. The oil and/or the free fatty acids obtained in the process are advantageously added to polymers, foodstuffs, feedstuffs, cosmetics, pharmaceuticals or products with industrial applications.
摘要翻译：本发明涉及增加转基因油料作物植物中总油含量和/或甘油3-磷酸盐含量的方法，其中基于总脂肪酸含量，优选在植物种子中，包含至少20重量％的油酸，通过从酵母，优选来自酿酒酵母（Saccharomyces cerevisiae）表达甘油3-磷酸脱氢酶（G3PDH）。在该方法中获得的油和/或游离脂肪酸有利地加入到具有工业应用的聚合物，食品，饲料，化妆品，药物或产品中。

8. 发明申请

US20070209087A1 Sugar and lipid metabolism regulators in plants VI 失效
标题翻译：植物中糖和脂质代谢调节剂六
公开(公告)号：US20070209087A1
公开(公告)日：2007-09-06
申请号：US11788342
申请日：2007-04-19
申请人： Oliver Oswald , Jorg Bauer , Thorsten Zank
发明人： Oliver Oswald , Jorg Bauer , Thorsten Zank
IPC分类号： C12N15/29 , C07H21/04 , C12N15/82
CPC分类号： C12N15/8247 , C12N15/8245
摘要： Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana, Brassica napus, and Helianthus annuus are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches, or seed storage proteins.
摘要翻译：提供了与脂质和糖代谢调节相关的分离的核酸和蛋白质。特别地，提供脂质代谢蛋白（LMP）和编码来自拟南芥，甘蓝型油菜和向日葵的核酸。核酸和蛋白质用于产生转基因植物和调节种子储存化合物水平的方法中。优选地，种子储存化合物是脂质，脂肪酸，淀粉或种子储存蛋白。

9. 发明申请

US20050155107A1 Sugar and lipid metabolism regulators in plants VI 失效
标题翻译：植物中糖和脂质代谢调节剂六
公开(公告)号：US20050155107A1
公开(公告)日：2005-07-14
申请号：US11021619
申请日：2004-12-23
申请人： Oliver Oswald , Jorg Bauer , Thorsten Zank
发明人： Oliver Oswald , Jorg Bauer , Thorsten Zank
IPC分类号： C12N15/82 , A01H1/00 , C07H21/04 , C12N5/04 , C12N9/10
CPC分类号： C12N15/8247 , C12N15/8245
摘要： Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana, Brassica napus, and Helianthus annuus are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches, or seed storage proteins.
摘要翻译：提供了与脂质和糖代谢调节相关的分离的核酸和蛋白质。特别地，提供脂质代谢蛋白（LMP）和编码来自拟南芥，甘蓝型油菜和向日葵的核酸。核酸和蛋白质用于产生转基因植物和调节种子储存化合物水平的方法中。优选地，种子储存化合物是脂质，脂肪酸，淀粉或种子储存蛋白。

10. 发明授权

US07537920B2 Method for the production of polyunsaturated fatty acids 有权
标题翻译：生产多不饱和脂肪酸的方法
公开(公告)号：US07537920B2
公开(公告)日：2009-05-26
申请号：US10547447
申请日：2004-01-29
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
IPC分类号： C12N9/12 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
摘要翻译：本发明涉及通过将核酸引入生物体中来生产多不饱和脂肪酸的方法，该生物体编码具有酰基-CoA：溶血磷脂一羧酰基转移酶活性的多肽。有利地，如果合适的话，这些核酸序列可以与编码脂肪酸或脂质代谢的生物合成多肽的其它核酸序列一起在转基因生物中表达。本发明还涉及核酸序列，包含本发明的核酸序列的核酸构建体，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物，核酸构建体和/或载体。本发明的另一部分涉及通过本发明的方法生产的油，脂和/或脂肪酸及其用途。

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