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1. 发明申请

US20070028326A1 Method for producing multiple unsaturated fatty acids in plants 有权
标题翻译：在植物中生产多种不饱和脂肪酸的方法
公开(公告)号：US20070028326A1
公开(公告)日：2007-02-01
申请号：US10511621
申请日：2003-04-25
申请人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
发明人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
IPC分类号： A01H1/00 , C12N9/04 , C12N15/82 , C12N5/04
CPC分类号： C12P7/6427 , C12N9/0083 , C12N15/8247 , C12P7/6472 , C12P7/6481
摘要： The present invention relates to a method for the production of fatty acid esters which comprise unsaturated fatty acids with at least three double bonds, and to free unsaturated fatty acids with a content of at least 1% by weight based on the total fatty acids present in the plants, by expressing at least one nucleic acid sequence which encodes a polypeptide with Δ6-desaturase activity and at least one nucleic acid sequence which encodes a polypeptide with Δ6-elongase activity. Advantageously, these nucleic acid sequences can, if appropriate, be expressed in the transgenic plant together with a third nucleic acid sequence which encodes a polypeptide with Δ5-desaturase activity. The invention furthermore relates to the use of defined nucleic acid sequences which encode polypeptides with a Δ6-desaturase activity, Δ6-elongase activity or Δ5-desaturase activity selected from a group of nucleic acid sequences, and/or to the use of nucleic acid constructs comprising the abovementioned nucleic acid sequences.
摘要翻译：本发明涉及一种生产脂肪酸酯的方法，所述脂肪酸酯包含具有至少三个双键的不饱和脂肪酸，以及游离不饱和脂肪酸，所述不饱和脂肪酸的含量为至少1重量％通过表达至少一个编码具有Δ6-去饱和酶活性的多肽的核酸序列和至少一个编码具有Δ6-延长酶活性的多肽的核酸序列的植物。有利地，如果合适，这些核酸序列可以与编码具有Δ5-去饱和酶活性的多肽的第三核酸序列一起在转基因植物中表达。本发明还涉及定义的编码具有Δ6-去饱和酶活性的多肽，Δ6-延长酶活性或选自一组核酸序列的Δ5-去饱和酶活性的定义的核酸序列的用途，和/或使用核酸构建体包含上述核酸序列。

2. 发明授权

US07893320B2 Method for producing multiple unsaturated fatty acids in plants 有权
标题翻译：在植物中生产多种不饱和脂肪酸的方法
公开(公告)号：US07893320B2
公开(公告)日：2011-02-22
申请号：US10511621
申请日：2003-04-25
申请人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
发明人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12P7/6427 , C12N9/0083 , C12N15/8247 , C12P7/6472 , C12P7/6481
摘要： The present invention relates to a method for the production of fatty acid esters which comprise unsaturated fatty acids with at least three double bonds, and to free unsaturated fatty acids with a content of at least 1% by weight based on the total fatty acids present in the plants, by expressing at least one nucleic acid sequence which encodes a polypeptide with Δ6-desaturase activity and at least one nucleic acid sequence which encodes a polypeptide with Δ6-elongase activity. Advantageously, these nucleic acid sequences can, if appropriate, be expressed in the transgenic plant together with a third nucleic acid sequence which encodes a polypeptide with Δ5-desaturase activity. The invention furthermore relates to the use of defined nucleic acid sequences which encode polypeptides with a Δ6-desaturase activity, Δ6-elongase activity or Δ5-desaturase activity selected from a group of nucleic acid sequences, and/or to the use of nucleic acid constructs comprising the abovementioned nucleic acid sequences.
摘要翻译：本发明涉及一种生产脂肪酸酯的方法，所述脂肪酸酯包含具有至少三个双键的不饱和脂肪酸，以及游离不饱和脂肪酸，所述不饱和脂肪酸的含量为至少1重量％通过表达编码具有＆Dgr.6-去饱和酶活性的多肽的至少一个核酸序列和至少一个编码具有＆Dgr.6-延伸酶活性的多肽的核酸序列的植物。有利的是，如果合适，这些核酸序列可以与编码具有＆Dgr。5-去饱和酶活性的多肽的第三核酸序列一起在转基因植物中表达。本发明进一步涉及定义的核酸序列的用途，所述核酸序列编码具有选自一组核酸序列的＆Dgr .6-去饱和酶活性和/或Dgr。5-去饱和酶活性的多肽，和/或使用包含上述核酸序列的核酸构建体。

3. 发明授权

US07544859B2 Elongase gene and method for producing multiple-unsaturated fatty acids 有权
标题翻译：延长酶基因及多不饱和脂肪酸的制备方法
公开(公告)号：US07544859B2
公开(公告)日：2009-06-09
申请号：US10182634
申请日：2001-02-08
申请人： Ernst Heinz , Thorsten Zank , Ulrich Zähringer , Jens Lerchl , Andreas Renz
发明人： Ernst Heinz , Thorsten Zank , Ulrich Zähringer , Jens Lerchl , Andreas Renz
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04
CPC分类号： C12N9/1029 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The invention relates to novel elongase genes with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.
摘要翻译：本发明涉及具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7所示序列的新型延伸酶基因或其同源物，衍生物或类似物，其包含该基因构建体基因或其同系物，衍生物和类似物及其用途。本发明还涉及包含具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的延伸酶基因或其同源物，衍生物和类似物的载体或转基因生物。本发明还涉及单独使用延伸酶基因序列或与其它延伸酶和/或其它脂肪酸生物合成基因组合的用途。本发明涉及具有序列SEQ ID NO：1或其同系物，衍生物和类似物的新型延伸酶基因。此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

4. 发明授权

US08088974B2 Production of polyunsaturated fatty acids, novel biosynthesis genes, and novel plant expression constructs 有权
标题翻译：生产多不饱和脂肪酸，新型生物合成基因和新型植物表达构建体
公开(公告)号：US08088974B2
公开(公告)日：2012-01-03
申请号：US12565799
申请日：2009-09-24
申请人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
发明人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
IPC分类号： C12N15/82 , A01H5/00 , C07H21/04
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 11 encoding polypeptides having desaturase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.
摘要翻译：本发明涉及生产具有至少两个双键的不饱和脂肪酸的方法。本发明还涉及编码在该方法中具有去饱和酶活性的多肽的核酸序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：11，并且用于产生转基因生物，优选地具有不饱和C18-，C20-或C22-脂肪酸的脂肪酸，油或脂质含量增加的转基因植物或转基因微生物及其同源物或衍生物，包括这些基因的基因构建体，以及它们单独使用或与多不饱和脂肪酸的生物合成基因组合使用。本发明还涉及用于种子特异性表达的多表达盒以及包含去饱和酶基因单独或与进一步去饱和酶和/或延伸酶基因或使用所述表达盒的同源物组合的载体或生物体。

5. 发明授权

US07615679B2 Method of producing polyunsaturated fatty acids, novel biosynthesis genes, and novel plant expression constructs 失效
标题翻译：生产多不饱和脂肪酸的方法，新型生物合成基因和新型植物表达构建体
公开(公告)号：US07615679B2
公开(公告)日：2009-11-10
申请号：US10250553
申请日：2002-01-18
申请人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
发明人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
IPC分类号： C12N15/82 , A01H5/00 , C07H21/04
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention further relates to the use of the nucleic acid sequence of SEQ ID NO: 1 encoding a polypeptide having delta-5 desaturase activity in the method and for generating a transgenic organism, preferably a transgenic plant or microorganism with an increased content of fatty acids, oil or lipids with unsaturated long chain fatty acids, to their homologs or derivatives, and to gene constructs encompassing these genes. The invention also relates to the use of the nucleic acid sequences alone or in combination with further desaturases and/or elongase genes in the method and for generating transgenic organisms.
摘要翻译：本发明涉及生产具有至少两个双键的不饱和脂肪酸的方法。本发明还涉及编码具有Δ-5去饱和酶活性的多肽的SEQ ID NO：1的核酸序列在该方法中的用途，并且用于产生转基因生物，优选具有增加的脂肪酸含量的转基因植物或微生物，具有不饱和长链脂肪酸的油或脂质，其同系物或衍生物，以及包含这些基因的基因构建体。本发明还涉及本方法中单核酸序列或其它去饱和酶和/或延伸酶基因与生成转基因生物体的组合的用途。

6. 发明申请

US20080160054A1 Novel elongase gene, and process for the preparation of polyunsaturated fatty acids 审中-公开
标题翻译：新型延伸酶基因，以及制备多不饱和脂肪酸的方法
公开(公告)号：US20080160054A1
公开(公告)日：2008-07-03
申请号：US11682269
申请日：2007-03-05
申请人： Ernst Heinz , Thorsten Zank , Ulrich Zahringer , Jens Lerchl , Andreas Renz
发明人： Ernst Heinz , Thorsten Zank , Ulrich Zahringer , Jens Lerchl , Andreas Renz
IPC分类号： A61K8/02 , C07H21/04 , C07K16/00 , C12N15/00 , C12N5/00 , A23D7/00 , C12N5/02 , C12P21/04 , G01N33/53 , A61K36/00
CPC分类号： C12N9/1029 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The invention relates to a novel elongase gene with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs.Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.
摘要翻译：本发明涉及具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7所示序列的新型延伸酶基因或其同源物，衍生物或类似物，其包含该基因或其同系物，衍生物和类似物，以及其用途。本发明还涉及包含具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的延伸酶基因或其同源物，衍生物和类似物的载体或转基因生物。本发明还涉及单独使用延伸酶基因序列或与其它延伸酶和/或其它脂肪酸生物合成基因组合的用途。本发明涉及具有序列SEQ ID NO：1或其同系物，衍生物和类似物的新型延伸酶基因。此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

7. 发明授权

US08933300B2 Elongase gene, and process for the preparation of polyunsaturated fatty acids 有权
标题翻译：延长酶基因，以及制备多不饱和脂肪酸的方法
公开(公告)号：US08933300B2
公开(公告)日：2015-01-13
申请号：US11682269
申请日：2007-03-05
申请人： Ernst Heinz , Thorsten Zank , Ulrich Zähringer , Jens Lerchl , Andreas Renz
发明人： Ernst Heinz , Thorsten Zank , Ulrich Zähringer , Jens Lerchl , Andreas Renz
IPC分类号： C12N15/82 , A01H5/00 , C12N1/20 , C07H21/04 , C12N9/10 , C12P7/64
CPC分类号： C12N9/1029 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The invention relates to novel elongase genes with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.
摘要翻译：本发明涉及具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的序列的新型延伸酶基因或其同源物，衍生物或类似物，其包含该基因构建体基因或其同系物，衍生物和类似物及其用途。本发明还涉及包含具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的延伸酶基因或其同源物，衍生物和类似物的载体或转基因生物。本发明还涉及单独使用延伸酶基因序列或与其它延伸酶和/或其它脂肪酸生物合成基因组合的用途。本发明涉及具有序列SEQ ID NO：1或其同系物，衍生物和类似物的新型延伸酶基因。此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

8. 发明授权

US08729337B2 Production of polyunsaturated fatty acids, novel biosynthesis genes, and novel plant expression constructs 有权
标题翻译：生产多不饱和脂肪酸，新型生物合成基因和新型植物表达构建体
公开(公告)号：US08729337B2
公开(公告)日：2014-05-20
申请号：US13342142
申请日：2012-01-02
申请人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
发明人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
IPC分类号： A01H5/00 , C12N15/82 , C12N1/20 , C07H21/04
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, 3, 5, 9, and 11 encoding polypeptides having desaturase or elongase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.
摘要翻译：本发明涉及生产具有至少两个双键的不饱和脂肪酸的方法。本发明还涉及在该方法中编码具有去饱和酶或延长酶活性的多肽的核酸序列SEQ ID NO：1,3,5,9和11的用途，并用于产生转基因生物，优选转基因植物或转基因微生物，具有不饱和C18-，C20-或C22-脂肪酸的脂肪酸，油脂或脂质以及它们的同系物或衍生物，包括这些基因的基因构建物的含量增加，以及它们单独使用或与生物合成相结合多不饱和脂肪酸的基因。本发明还涉及用于种子特异性表达的多表达盒以及包含去饱和酶基因单独或与进一步去饱和酶和/或延伸酶基因或使用所述表达盒的同源物组合的载体或生物体。

9. 发明申请

US20120210465A1 Production of Polyunsaturated Fatty Acids, Novel Biosynthesis Genes, And Novel Plant Expression Constructs 有权
标题翻译：多不饱和脂肪酸的生产，新型生物合成基因和新型植物表达构建体
公开(公告)号：US20120210465A1
公开(公告)日：2012-08-16
申请号：US13342142
申请日：2012-01-02
申请人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
发明人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
IPC分类号： C12P7/64 , C12N1/13 , C12N1/21 , C12N1/11 , C12N5/10 , C07H21/04 , C12N1/19 , C12N9/02 , C12N9/00 , C07C69/007 , A01H5/00 , A01H1/06 , C12N15/63 , C12N15/52
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, 3, 5, 9, and 11 encoding polypeptides having desaturase or elongase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.
摘要翻译：本发明涉及生产具有至少两个双键的不饱和脂肪酸的方法。本发明还涉及在该方法中编码具有去饱和酶或延长酶活性的多肽的核酸序列SEQ ID NO：1,3,5,9和11的用途，并用于产生转基因生物，优选转基因植物或转基因微生物，具有不饱和C18-，C20-或C22-脂肪酸的脂肪酸，油脂或脂质以及它们的同系物或衍生物，包括这些基因的基因构建物的含量增加，以及它们单独使用或与生物合成相结合多不饱和脂肪酸的基因。本发明还涉及用于种子特异性表达的多表达盒以及包含去饱和酶基因单独或与进一步去饱和酶和/或延伸酶基因或使用所述表达盒的同源物组合的载体或生物体。

10. 发明申请

US20100021976A1 PRODUCTION OF POLYUNSATURATED FATTY ACIDS, NOVEL BIOSYNTHESIS GENES, AND NOVEL PLANT EXPRESSION CONSTRUCTS 有权
标题翻译：聚氧乙烯脂肪酸生产，新型生物基因和新型植物表达构造
公开(公告)号：US20100021976A1
公开(公告)日：2010-01-28
申请号：US12565799
申请日：2009-09-24
申请人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zahringer
发明人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zahringer
IPC分类号： C12P7/64 , C12N15/53 , C12N15/74 , A01H5/00 , C12N5/10 , C12N1/00 , C12N1/19 , C12N15/82 , C12N15/85 , C12N15/81
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 11 encoding polypeptides having desaturase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.
摘要翻译：本发明涉及生产具有至少两个双键的不饱和脂肪酸的方法。本发明还涉及编码在该方法中具有去饱和酶活性的多肽的核酸序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：11，并且用于产生转基因生物，优选地具有不饱和C18-，C20-或C22-脂肪酸的脂肪酸，油或脂质含量增加的转基因植物或转基因微生物及其同源物或衍生物，包括这些基因的基因构建体，以及它们单独使用或与多不饱和脂肪酸的生物合成基因组合使用。本发明还涉及用于种子特异性表达的多表达盒以及包含去饱和酶基因单独或与进一步去饱和酶和/或延伸酶基因或使用所述表达盒的同源物组合的载体或生物体。

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