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1. 发明申请

US20100264099A1 SEPARATION DEVICE COMPRISING A PHYSICAL BARRIER 审中-公开
标题翻译：包含物理障碍物的分离装置
公开(公告)号：US20100264099A1
公开(公告)日：2010-10-21
申请号：US12742386
申请日：2007-11-26
申请人： Peter Warthoe , Per Berdén
发明人： Peter Warthoe , Per Berdén
IPC分类号： B01D35/28 , B01D37/00
CPC分类号： B01L3/502753 , B01L2300/0681 , B01L2300/16 , B01L2400/0406
摘要： The present invention relates to a device for separating a suspension into a liquid phase and a retentate phase. The device comprises a separation chamber comprising an application zone and a hydrophilic filter material. The separation chamber is connected to a first capillary channel, where the connecting junction between the separation chamber and the first capillary channel comprise a physical barrier preventing flow of residue retentate from a lower part of the chamber into the first capillary channel. The invention further relates to a method for separating a liquid sample consisting of less than 200 μl suspension, into a retentate phase comprising the suspended matter, and a liquid phase substantially free of suspended matter.
摘要翻译：本发明涉及一种用于将悬浮液分离成液相和滞留相的装置。该装置包括分离室，其包括施加区域和亲水性过滤材料。分离室连接到第一毛细通道，其中分离室和第一毛细管通道之间的连接结包括物理屏障，防止残余物滞留物从腔室的下部流入第一毛细通道。本发明还涉及将由少于200μl悬浮液组成的液体样品分离成包含悬浮物的滞留相和基本上不含悬浮物的液相的方法。

2. 发明申请

US20090282902A1 BIO SURFACE ACOUSTIC WAVE (SAW) RESONATOR AMPLIFICATION FOR DETECTION OF A TARGET ANALYTE 审中-公开
标题翻译：用于检测目标分析仪的生物表面声波（SAW）谐振器放大
公开(公告)号：US20090282902A1
公开(公告)日：2009-11-19
申请号：US12310161
申请日：2007-08-17
申请人： Peter Warthoe
发明人： Peter Warthoe
IPC分类号： G01N29/02
CPC分类号： G01N33/54373 , G01N29/022 , G01N29/222 , G01N29/2437 , G01N2291/02466 , G01N2291/0255
摘要： The present invention relates generally to a signal amplification method for a SAW resonator microsensor for analyzing test samples, containing target analyte including proteins and nucleic acids. The invention relates to at least one surface acoustic wave resonator unit comprising a plurality of three-dimensional interdigital transducer electrode (IDTE) and reflector micro channels located on a piezoelectric substrate surface. The invention further relates to a change of solid/liquid volume ratio in said three-dimensional micro channels; said changes of the liquid/solid volume ratio can be directed correlated to the target analyte concentration in a test sample.
摘要翻译：本发明一般涉及用于分析测试样品的SAW谐振器微传感器的信号放大方法，其中包含目标分析物包括蛋白质和核酸。本发明涉及至少一个表面声波谐振器单元，其包括位于压电基片表面上的多个三维叉指式换能器电极（IDTE）和反射器微通道。本发明还涉及所述三维微通道中固体/液体体积比的变化; 所述液体/固体体积比的变化可以与测试样品中的目标分析物浓度相关。

3. 发明授权

US08153780B2 Reporter unit for detection of target molecules using polymerisable substrate 失效
标题翻译：用于使用可聚合底物检测靶分子的记录单元
公开(公告)号：US08153780B2
公开(公告)日：2012-04-10
申请号：US12449357
申请日：2008-02-06
申请人： Peter Warthoe
发明人： Peter Warthoe
IPC分类号： C07H19/04 , C12Q1/68 , C12P19/34
CPC分类号： G01N33/581
摘要： The invention relates to a reporter unit for detecting a target molecule comprising at least one component attached to a target molecule specific probe, wherein said at least one component is liberated from said probe by the activity of a first enzyme thereby making the at least one component available as a substrate for a second enzyme which employs the substrate in a polymerization reaction to obtain a detectable structure.
摘要翻译：本发明涉及用于检测靶分子的报告单元，其包含连接于靶分子特异性探针的至少一种成分，其中所述至少一种成分通过第一种酶的活性从所述探针释放，从而使至少一种成分可用作第二种酶的底物，其在聚合反应中使用该底物以获得可检测的结构。

4. 发明申请

US20100099089A1 REPORTER UNIT FOR DETECTION OF TARGET MOLECULES USING POLYMERISABLE SUBSTRATE 失效
标题翻译：使用可聚合基材检测目标分子的报告单元
公开(公告)号：US20100099089A1
公开(公告)日：2010-04-22
申请号：US12449357
申请日：2008-02-06
申请人： Peter Warthoe
发明人： Peter Warthoe
IPC分类号： C12Q1/68 , C12N9/00 , C12N9/16 , C12N9/10
CPC分类号： G01N33/581
摘要： The invention relates to a reporter unit for detecting a target molecule comprising at least one component attached to a target molecule specific probe, wherein said at least one component is liberated from said probe by the activity of a first enzyme thereby making the at least one component available as a substrate for a second enzyme which employs the substrate in a polymerization reaction to obtain a detectable structure.
摘要翻译：本发明涉及用于检测靶分子的报告单元，其包含连接于靶分子特异性探针的至少一种成分，其中所述至少一种成分通过第一种酶的活性从所述探针释放，从而使至少一种成分可用作第二种酶的底物，其在聚合反应中使用该底物以获得可检测的结构。

5. 发明申请

US20110045505A1 INTEGRATED SEPARATION AND DETECTION CARTRIDGE WITH MEANS AND METHOD FOR INCREASING SIGNAL TO NOISE RATIO 审中-公开
标题翻译：具有增加信号噪声比的方法和方法的集成分离和检测盒
公开(公告)号：US20110045505A1
公开(公告)日：2011-02-24
申请号：US12742830
申请日：2008-11-26
申请人： Peter Warthoe , Soren Mentzel , Klaus Rune Andersen , Jens Mikkelsen , Jacob Holst Madsen , Per Berdén
发明人： Peter Warthoe , Soren Mentzel , Klaus Rune Andersen , Jens Mikkelsen , Jacob Holst Madsen , Per Berdén
IPC分类号： G01N33/53 , C12M1/34 , B01L99/00
CPC分类号： B01L3/502753 , B01L3/502715 , B01L3/50273 , B01L2200/0631 , B01L2200/0647 , B01L2200/10 , B01L2300/0681 , B01L2300/0887 , B01L2300/161 , B01L2400/043 , G01N33/54326 , G01N33/54346 , G01N33/54373 , G01N33/54393
摘要： The present invention relates to a device and a method for quantitative detecting of the presence or absence of a target analyte in a liquid sample having a volume of less than 200 μl, the device comprising a reaction chamber in the form of a capillary channel, a first part comprising a sample inlet for the introduction of a sample containing an analyte, and a discharge outlet for the discharge of waste products; a second part comprising means for detection of the target analyte, and a solution inlet for introduction of washing solutions and reaction mixtures; and means for transferring an immobilized analyte from the first part to the second part of the chamber and vice versa, where the first and second parts are separated such that other liquid sample material may not enter the second part of the chamber and such that light may not be transferred from the first part of the chamber to the detector part of the second part of the chamber.
摘要翻译：本发明涉及一种用于定量检测体积小于200μl的液体样品中目标分析物的存在或不存在的装置和方法，该装置包括呈毛细通道形式的反应室，第一部分包括用于引入含有分析物的样品的样品入口和用于排出废物的排出口; 第二部分包括用于检测目标分析物的装置和用于引入洗涤溶液和反应混合物的溶液入口; 以及用于将固定的分析物从所述第一部分转移到所述室的第二部分的装置，反之亦然，其中所述第一和第二部分被分离，使得其它液体样品材料可能不会进入所述室的第二部分，不能从室的第一部分转移到室的第二部分的检测器部分。

6. 发明授权

US07473551B2 Nano-mechanic microsensors and methods for detecting target analytes 失效
标题翻译：纳米力学微传感器和检测目标分析物的方法
公开(公告)号：US07473551B2
公开(公告)日：2009-01-06
申请号：US11134821
申请日：2005-05-20
申请人： Peter Warthoe
发明人： Peter Warthoe
IPC分类号： G01N33/543
CPC分类号： G01N29/022 , G01N29/226 , G01N29/2462 , G01N29/30 , G01N2291/0255 , G01N2291/0256 , G01N2291/0422 , G01N2291/0423 , G01N2291/0427
摘要： The present invention relates generally to methods and compositions for analyzing test samples containing target analytes including proteins and nucleic acids. The invention uses a surface acoustic wave sensor in combination with a hydrogel to obtain an ultra sensitive non-fluorescent detection system.
摘要翻译：本发明一般涉及用于分析含有目标分析物（包括蛋白质和核酸）的测试样品的方法和组合物。本发明使用表面声波传感器与水凝胶组合以获得超灵敏的非荧光检测系统。

7. 发明申请

US20060024813A1 Nano-mechanic microsensors and methods for detecting target analytes 失效
公开(公告)号：US20060024813A1
公开(公告)日：2006-02-02
申请号：US11134821
申请日：2005-05-20
申请人： Peter Warthoe
发明人： Peter Warthoe
IPC分类号： G01N33/566 , G01N33/567
CPC分类号： G01N29/022 , G01N29/226 , G01N29/2462 , G01N29/30 , G01N2291/0255 , G01N2291/0256 , G01N2291/0422 , G01N2291/0423 , G01N2291/0427
摘要： The present invention relates generally to methods and compositions for analyzing test samples containing target analytes including proteins and nucleic acids. The invention uses a surface acoustic wave sensor in combination with a hydrogel to obtain an ultra sensitive non-fluorescent detection system.

8. 发明申请

US20150126382A1 TECHNIQUE COMBINING PCR AND LOOP-MEDIATED ISOTHERMAL AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACIDS 审中-公开
标题翻译：用于检测核酸的技术组合PCR和环介导的同位素放大
公开(公告)号：US20150126382A1
公开(公告)日：2015-05-07
申请号：US14400717
申请日：2013-05-13
申请人： Peter WARTHOE
发明人： Peter Warthoe
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6853 , C12Q1/689 , C12Q1/6895 , C12Q2525/301 , C12Q2531/119 , C12Q2600/16
摘要： The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a sequence of steps comprising an isothermal amplification of the pre-amplified sample, wherein the isothermal amplification comprises a pair of primers comprising a forward primer having a 3′ part that is substantially complementary to a first part of the target sequence, the presence or absence of which is to be detected, and a 5′ part that is substantially homolog to a second part of the target sequence, and a reverse primer comprising a 3′ part that is substantially homolog to a fourth part of the target sequence and a 5′ part that is substantially complementary to a third part of the target sequence.
摘要翻译：本发明涉及一种用于检测样品中一种或多种靶核酸序列的存在或不存在的部分的方法和试剂盒，所述方法包括通过聚合酶链反应预扩增样品的步骤序列之后是包括预扩增样品的等温扩增的步骤序列，其中等温扩增包括一对引物，其包含具有与靶序列的第一部分基本上互补的3'部分的正向引物，其目标序列的第二部分基本上同源的5'部分和与靶序列的第四部分基本相同的3'部分的反向引物，和 5'部分，其与目标序列的第三部分基本互补。

9. 发明申请

US20100105079A1 BIO SURFACE ACOUSTIC WAVE (SAW) RESONATOR AMPLIFICATION WITH NANOPARTICLES FOR DETECTION OF A TARGET ANALYTE 审中-公开
标题翻译：用于检测目标分析仪的纳米颗粒的生物表面声波（SAW）谐振器放大
公开(公告)号：US20100105079A1
公开(公告)日：2010-04-29
申请号：US12451611
申请日：2008-06-02
申请人： Peter Warthoe
发明人： Peter Warthoe
IPC分类号： G01N33/53
CPC分类号： G01N33/54373
摘要： The present invention relates generally to a signal amplification method for a SAW resonator microsensor for analyzing test samples, containing target analyte including proteins and nucleic acids. The method comprising the steps: reacting the analyte in a sample with a first molecular recognition component and a second molecular recognition component linked to at least one nanoparticle; and adding an enhancement solution comprising silver ions and/or gold ions, and a reducing agent, whereby the silver ions and/or gold ions are reduced to metallic silver and/or gold which is deposited onto the surface of the at least one nanoparticle; wherein the mass increase of the at least one nanoparticle is detected by a SAW sensor.
摘要翻译：本发明一般涉及用于分析测试样品的SAW谐振器微传感器的信号放大方法，其中包含目标分析物包括蛋白质和核酸。该方法包括以下步骤：将样品中的分析物与连接至至少一种纳米颗粒的第一分子识别组分和第二分子识别组分反应; 以及添加包含银离子和/或金离子的增强溶液和还原剂，由此将银离子和/或金离子还原成沉积在至少一种纳米颗粒的表面上的金属银和/或金; 其中所述至少一种纳米颗粒的质量增加由SAW传感器检测。

10. 发明授权

US06271004B1 Method for improved reverse transcription at high temperatures 失效
标题翻译：在高温下改进逆转录的方法
公开(公告)号：US06271004B1
公开(公告)日：2001-08-07
申请号：US09603185
申请日：2000-06-26
申请人： Peter Warthoe
发明人： Peter Warthoe
IPC分类号： C12P1934
CPC分类号： C12Q1/686 , C12Q2563/119 , C12Q2527/125 , C12Q2521/107
摘要： The invention relates to a method for enzyme stabilization. A method for improved reverse transcription at high temperatures is provided, wherein a thermostable heat shock protein (HSPs) stabilizes a reverse transcriptase, as well as reduces the RNase H activity of said reverse transcriptase. The present invention thus relates to a stabilizing agent, that prevents thermal denaturing and enhances thermostability of a reverse transcriptase. The invention further relates to a method of producing a polypeptide complex consisting of a Chaperonin and a Moloney murine leukemia virus (MMVL) reverse transcriptase, characterized by having enhanced thermostability as well as reduced RNase H activity, compared to a (MMVL) reverse transcriptase alone. The invention further relates to a kit for the preparation of cDNA from mRNA, comprising either both stabilizing agent and reverse transcriptase or the polypeptide complex of the invention.
摘要翻译：本发明涉及一种酶稳定的方法。提供了一种在高温下改善逆转录的方法，其中热稳定热休克蛋白（HSP）稳定逆转录酶，并降低所述逆转录酶的RNA酶H活性。因此，本发明涉及防止热变性并提高逆转录酶的热稳定性的稳定剂。本发明进一步涉及与单独的（MMVL）逆转录酶相比，由伴侣蛋白和莫洛尼鼠白血病病毒（MMVL）逆转录酶组成的多肽复合物的方法，其特征在于具有增强的热稳定性以及降低的RNA酶H活性。本发明还涉及用于从mRNA制备cDNA的试剂盒，其包含稳定剂和逆转录酶两者或本发明的多肽复合物。

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