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    • 9. 发明授权
    • Methods for the modulation of lignification levels
    • 木质素含量调节方法
    • US6087557A
    • 2000-07-11
    • US957941
    • 1992-10-06
    • Monika ClausenChristopher J. LambPeter DoernerYonatan ElkindRoland Megnet
    • Monika ClausenChristopher J. LambPeter DoernerYonatan ElkindRoland Megnet
    • C12N9/88C12N15/82A01H5/00
    • C12N15/8255C12N9/88
    • In accordance with the present invention, there are provided methods to modulate plant lignification. For example, the level of the enzyme phenylalanine ammonia-lyase (PAL), a key enzyme in the production of lignin precursors, can be increased or decreased, as desired, to increase or decrease lignification levels. Alternatively, the level of lignin precursors in the plant can be modulated either by enhancing the plant's ability to produce such precursors, or by causing the compounds which are required for the production of lignin precursors to undergo alternative chemical conversions (which prevent the incorporation of such compounds into lignin by diverting the lignin precursors to other chemical pathways). In addition, there is provided a method for producing plants having modified lignin composition and content, by converting a portion of the cinnamic acid in said plant to styrene. In another embodiment, there is provided a method for producing styrene and/or styrene derivatives, by contacting cinnamic acid or a cinnamic acid derivative with PAD under decarboxylation conditions.
    • 根据本发明,提供了调节植物木质素化的方法。 例如,木质素前体生产中的关键酶苯丙氨酸氨裂解酶(PAL)的水平可根据需要增加或降低以增加或减少木质素含量。 或者,植物中木质素前体的水平可以通过提高植物产生这种前体的能力或通过使生产木质素前体所需的化合物经历替代的化学转化来调节(这阻止了这种掺入 通过将木质素前体转移到其他化学途径将化合物转化成木质素)。 此外,提供了通过将所述植物中的部分肉桂酸转化成苯乙烯来生产具有改性的木质素组成和含量的植物的方法。 在另一个实施方案中,提供了通过在脱羧条件下将肉桂酸或肉桂酸衍生物与PAD接触来制备苯乙烯和/或苯乙烯衍生物的方法。