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    • 2. 发明授权
    • Sequential solid phase immunoassay including contact pad to limit conjugate flow during manufacture
    • 连续固相免疫测定,包括接触垫,以限制制造期间的共轭流动
    • US08501495B2
    • 2013-08-06
    • US12704147
    • 2010-02-11
    • Kai Ling YaoPeter Chun
    • Kai Ling YaoPeter Chun
    • G01N33/558G01N33/563G01N33/53C12N1/34
    • G01N33/54333
    • A sequential solid phase immunoassay and system is disclosed. The immunoassay utilizes the secondary antibody method for the detection of antibodies in a membrane-based test. The system comprises a test strip including a nitrocellulose membrane having an immobilized antigen in a capture zone on the membrane and a stabilized liquid secondary antibody conjugate. The sequential solid phase immunoassay is performed in a sequential manner with the addition of a fluid specimen being followed by the addition of the stabilized liquid secondary antibody conjugate. The sequential procedure using the system includes allowing the fluid specimen containing antibodies specific to the antigen to pass laterally from the test strip first end through the capture zone. The immobilized antigens in the capture zone capture antibodies specific to the antigen. The stabilized liquid secondary antibody conjugate then binds to the captured antibodies and can be detected visually or by a machine or reader.
    • 公开了连续固相免疫测定和系统。 免疫测定法利用二抗法在基于膜的检测中检测抗体。 该系统包括测试条,其包括在膜上的捕获区中具有固定化抗原的硝酸纤维素膜和稳定的液体二抗缀合物。 顺序的固相免疫测定是按照顺序的方式进行的,随后加入流体样品后加入稳定的液体二抗偶联物。 使用该系统的顺序方法包括允许含有抗原特异性抗体的流体样品从测试条第一端横向通过捕获区。 捕获区域中的固定化抗原捕获抗原特异性的抗体。 稳定的液体二抗缀合物然后结合捕获的抗体,并且可以通过目视检测或通过机器或读取器检测。
    • 6. 发明申请
    • SEQUENTIAL SOLID PHASE IMMUNOASSAY AND SYSTEM THEREFOR
    • 序列固定相免疫系统及其系统
    • US20100144061A1
    • 2010-06-10
    • US12704147
    • 2010-02-11
    • Kai Ling YaoPeter Chun
    • Kai Ling YaoPeter Chun
    • G01N33/543
    • G01N33/54333
    • A sequential solid phase immunoassay and system is disclosed. The immunoassay utilizes the secondary antibody method for the detection of antibodies in a membrane-based test. The system comprises a test strip including a nitrocellulose membrane having an immobilized antigen in a capture zone on the membrane and a stabilized liquid secondary antibody conjugate. The sequential solid phase immunoassay is performed in a sequential manner with the addition of a fluid specimen being followed by the addition of the stabilized liquid secondary antibody conjugate. The sequential procedure using the system includes allowing the fluid specimen containing antibodies specific to the antigen to pass laterally from the test strip first end through the capture zone. The immobilized antigens in the capture zone capture antibodies specific to the antigen. The stabilized liquid secondary antibody conjugate then binds to the captured antibodies and can be detected visually or by a machine or reader.
    • 公开了连续固相免疫测定和系统。 免疫测定法利用二抗法在基于膜的检测中检测抗体。 该系统包括测试条,其包括在膜上的捕获区中具有固定化抗原的硝酸纤维素膜和稳定的液体二抗缀合物。 顺序的固相免疫测定是按照顺序的方式进行的,随后加入流体样品后加入稳定的液体二抗偶联物。 使用该系统的顺序方法包括允许含有抗原特异性抗体的流体样品从测试条第一端横向通过捕获区。 捕获区域中的固定化抗原捕获抗原特异性的抗体。 稳定的液体二抗缀合物然后结合捕获的抗体,并且可以通过目视检测或通过机器或读取器检测。