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    • 3. 发明申请
    • Insecticidal toxin complex fusion proteins
    • 杀虫毒素复合融合蛋白
    • US20060168683A1
    • 2006-07-27
    • US11070573
    • 2005-03-02
    • Timothy HeyThomas MeadeStephanie BurtonDonald MerloQihua CaiHaley MoonJoel SheetsAaron Woosley
    • Timothy HeyThomas MeadeStephanie BurtonDonald MerloQihua CaiHaley MoonJoel SheetsAaron Woosley
    • A01H1/00C07K14/415C12N15/82C12N5/04
    • C12N15/8286C07K14/195C07K14/24C07K14/32C07K2319/00C07K2319/55C12N15/62Y02A40/162
    • The subject invention relates to insecticidal toxin complex (“TC”) fusion proteins and to polynucleotides that encode these fusion proteins. The subject invention also includes polynucleotides that encode the subject TC fusion proteins, and vectors comprising said polynucleotides. In some embodiments, the invention provides a fusion protein comprising a Class A protein, a Class B protein, and a Class C TC protein fused together to form a single protein. In some other embodiments, the invention provides a fusion protein comprising a Class B and a Class C TC proteins fused together. In the latter embodiments, the BC or CB fusion protein can be used to enhance or potentiate the anti-insect activity of a “Toxin A” or Class A protein. Heretofore, there was no expectation that such fusion proteins would properly function and retain their activity when fused together. The subject invention advantageously reduces the number of genes needed to transform plants. Thus, the subject invention also includes plants, cells (bacterial and plant cells for example), and seeds that comprise said polynucleotides. Said plants can produce fusion proteins of the subject invention, which convey insect resistance to said plants. The subject invention also includes methods of controlling pests (preferably insects and other plant pests) with fusion proteins of the subject invention.
    • 本发明涉及杀虫毒素复合物(“TC”)融合蛋白和编码这些融合蛋白的多核苷酸。 本发明还包括编码受试者TC融合蛋白的多核苷酸和包含所述多核苷酸的载体。 在一些实施方案中,本发明提供了融合蛋白,其包含一起融合以形成单一蛋白质的A类蛋白质,B类蛋白质和C类TC蛋白质。 在一些其他实施方案中,本发明提供了融合蛋白,其包含融合在一起的B类和C类TC蛋白。 在后一个实施方案中,BC或CB融合蛋白可用于增强或增强“毒素A”或A类蛋白的抗昆虫活性。 迄今为止,没有任何期望,当融合在一起时,这种融合蛋白将适当地起作用并保持其活性。 本发明有利地减少转化植物所需的基因数量。 因此,本发明还包括植物,细胞(例如细菌和植物细胞)和包含所述多核苷酸的种子。 所述植物可产生本发明的融合蛋白,其向所述植物传递昆虫抗性。 本发明还包括用本发明的融合蛋白控制害虫(优选昆虫和其它植物害虫)的方法。