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    • 5. 发明授权
    • Thin reaction chambers for containing and handling liquid microvolumes
    • 用于容纳和处理液体微量的薄反应室
    • US5922604A
    • 1999-07-13
    • US869491
    • 1997-06-05
    • Marilyn StapletonWilliam Harrington
    • Marilyn StapletonWilliam Harrington
    • B01L3/00G02B21/34G01N1/18G01N21/05
    • G02B21/34B01L3/5027B01L3/502723B01L3/508B01L2200/0642B01L2200/0684B01L2200/142B01L2300/069B01L2300/0822B01L2400/0406B01L3/502715B01L3/50273B01L3/5085Y10T436/112499
    • The invention is an apparatus and a process for containing and handling liquids in a thin capillary space. Two opposing walls positioned at a first and a second distance apart define a thin space having at least two different heights and two different regions of capillary liquid attraction. Enclosing the thin space with side walls defines a reaction chamber. A liquid receiving area, entry and exit ports and features projecting from, or encircling, at least one wall allow coatings on the internal surface of either of the two opposing walls to react with multiple microvolumes of reagents and wash treatments in series. Liquids flow into, or are withdrawn from, the thin space in a way in which bubbles are not introduced into the thin space. Bubbles that may arise in the thin space move along a sloped feature out of the thin space into an annular enclosure. The liquids are withdrawn after reacting with the coatings, either to empty the chamber and recover the liquid volume, or to wash the coatings with treatment liquids and introduce new treatment liquids. The ability to control liquid movement in spaces thin enough to exhibit capillarity is useful for analyzing biological specimens cost-effectively with less reagent volume and more information per specimen and without sacrificing convenience in liquid handling or surface reaction space.
    • 本发明是用于在薄毛细管空间中容纳和处理液体的装置和方法。 位于隔开第一和第二距离的两个相对的壁限定了具有至少两个不同高度和两个不同毛细管吸液区域的薄空间。 用侧壁封闭薄空间限定了反应室。 液体接收区域,入口和出口端口以及从至少一个壁突出或环绕的特征允许两个相对壁中的任一个的内表面上的涂层与多个微体积的试剂和串联的洗涤处理反应。 液体以不会将气泡引入薄空间的方式流入或从薄空间排出。 可能在薄空间中出现的气泡沿着倾斜的特征从薄空间移动到环形外壳中。 在与涂层反应后取出液体,以清空室并回收液体体积,或用处理液洗涤涂层并引入新的处理液体。 控制液体运动在足够薄到足以显示毛细作用的空间的能力可用于分析生物样本,以较少的试剂体积和更多的样品信息分析生物样品,并且不会牺牲液体处理或表面反应空间的便利性。