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1. 发明申请

WO2004035170A3 COMPOSITION FOR SEPARATING MOLECULES 审中-公开
标题翻译：用于分离分子的组合物
公开(公告)号：WO2004035170A3
公开(公告)日：2005-08-04
申请号：PCT/US0333220
申请日：2003-10-20
申请人： PROMEGA CORP , SIMPSON DANIEL J , JOHNSON TONNY , FLEMMING RODERICK G
发明人： SIMPSON DANIEL J , JOHNSON TONNY , FLEMMING RODERICK G
IPC分类号： B01J20/22 , B01J20/32 , B01J45/00 , C02F1/28 , C07K1/20 , C12N9/00
CPC分类号： B01D15/3828 , B01J20/103 , B01J20/28009 , B01J20/286 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3234 , B01J20/3242 , B01J20/3251 , B01J20/3259 , B01J20/3265 , B01J20/3289 , B01J20/3293 , B01J45/00 , B01J2220/58 , B01J2220/66 , C02F1/285 , C02F2101/20 , C07K1/20 , G01N2030/042
摘要： The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.
摘要翻译：本发明提供了使用改性固体支持物分离金属或生物分子例如多肽，核酸或内毒素的组合物。

2. 发明申请

WO2004036189A3 METHODS FOR SEPARATING MOLECULES 审中-公开
标题翻译：分离分子的方法
公开(公告)号：WO2004036189A3
公开(公告)日：2005-09-01
申请号：PCT/US0333374
申请日：2003-10-20
申请人： PROMEGA CORP , SIMPSON DANIEL J , JOHNSON TONNY , SHULTZ JOHN , FLEMMING RODERICK G , GODAT REBECCA , KAR SANCHAYITA , HURST ROBIN
发明人： SIMPSON DANIEL J , JOHNSON TONNY , SHULTZ JOHN , FLEMMING RODERICK G , GODAT REBECCA , KAR SANCHAYITA , HURST ROBIN
IPC分类号： B01J20/22 , B01J20/32 , B01J45/00 , C02F1/28 , C07K1/20 , C12N9/00
CPC分类号： B01D15/3828 , B01J20/103 , B01J20/28009 , B01J20/286 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3234 , B01J20/3242 , B01J20/3251 , B01J20/3259 , B01J20/3265 , B01J20/3289 , B01J20/3293 , B01J45/00 , B01J2220/58 , B01J2220/66 , C02F1/285 , C02F2101/20 , C07K1/20 , G01N2030/042
摘要： The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.
摘要翻译：本发明提供使用金属改性固体支持物分离金属或分子例如多肽，核酸或内毒素的组合物和方法。使用本发明的改性固体载体，从原料中分离金属或分子。本发明还提供了可以结合本发明方法使用的试剂盒。

3. 发明申请

WO0069872A3 pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS 审中-公开
标题翻译： pH依赖离子交换矩阵及其在核酸分离中的使用方法
公开(公告)号：WO0069872A3
公开(公告)日：2001-02-15
申请号：PCT/US0012186
申请日：2000-05-05
申请人： PROMEGA CORP
发明人： SMITH CRAIG E , HOLMES DIANA L , SIMPSON DANIEL J , KATZHENDLER JEHOSHUA , BITNER REX M , GROSCH JOSEPHINE C
IPC分类号： G01N30/00 , B01D15/04 , B01D15/08 , B01J20/10 , B01J20/22 , B01J20/286 , B01J39/26 , B01J41/20 , B01J43/00 , C07H21/00 , C12N15/09 , C12N15/10 , G01N30/88 , B01J41/06
CPC分类号： C12N15/101 , B01J39/26 , B01J41/20
摘要： pH dependent ion exchange matrices are provided, with methods for making such matrices, and methods for using such matrices to isolate a target nucleic acid, as such as plasmid DNA, chromosomal DNA, or RNA from contaminants, including proteins, lipids, cellular debris, or other nucleic acids. Each pH dependent ion exchange matrix of this invention comprises at least two different ion exchange functional groups, one of which is capable of acting as an anion exchanger at a first pH, and the other of which is capable of acting as a cation exchanger at a second, higher pH. The matrix has an overall neutral charge in a pH range between the first and second pH. The pH dependent ion exchange matrices of the present invention are designed to bind to the target nucleic acid at a pH wherein the overall charge of the matrix is positive, and to release the target nucleic acid as the pH of the surrounding solution is increased. The target nucleic acid can be released from the pH dependent matrix in little or no salt and at about a neutral pH. The matrices and methods of this invention enable one to isolate a target nucleic acid in very few steps, without the use of hazardous chemicals. Target nucleic acids isolated using the pH dependent ion exchange matrices according to the present invention can be used immediately without further extraction or isolation.
摘要翻译：提供了pH依赖性离子交换矩阵，以及用于制备这种基质的方法，以及使用这种基质从污染物（包括蛋白质，脂质，细胞碎片）分离靶核酸（例如质粒DNA，染色体DNA或RNA）的方法，或其他核酸。本发明的每个pH依赖性离子交换基质包含至少两种不同的离子交换官能团，其中之一能够在第一pH下作为阴离子交换剂，另一种能够在第二，较高的pH值。该基质在第一和第二pH之间的pH范围内具有总体中性电荷。本发明的pH依赖性离子交换基质被设计成在其中基质的总电荷为阳性的pH下与靶核酸结合，并且随着周围溶液的pH增加而释放靶核酸。目标核酸可以很少或没有盐和约中性pH从pH依赖性基质释放。本发明的基质和方法使得能够以非常少的步骤分离靶核酸，而不使用危险化学品。使用根据本发明的pH依赖性离子交换基质分离的靶核酸可以立即使用，无需进一步提取或分离。

4. 发明专利

CA2372054A1 PH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS 未知
公开(公告)号：CA2372054A1
公开(公告)日：2000-11-23
申请号：CA2372054
申请日：2000-05-05
申请人： PROMEGA CORP
发明人： GROSCH JOSEPHINE C , BITNER REX M , KATZHENDLER JEHOSHUA , SIMPSON DANIEL J , SMITH CRAIG E , HOLMES DIANA L
IPC分类号： G01N30/00 , B01D15/04 , B01D15/08 , B01J20/10 , B01J20/22 , B01J20/286 , B01J39/26 , B01J41/20 , B01J43/00 , C07H21/00 , C12N15/09 , C12N15/10 , G01N30/88 , B01J41/06
摘要： pH dependent ion exchange matrices are provided, with methods for making suc h matrices, and methods for using such matrices to isolate a target nucleic acid, as such as plasmid DNA, chromosomal DNA, or RNA from contaminants, including proteins, lipids, cellular debris, or other nucleic acids. Each pH dependent ion exchange matrix of this invention comprises at least two different ion exchange functional groups, one of which is capable of acting as an anion exchanger at a first pH, and the other of which is capable of actin g as a cation exchanger at a second, higher pH. The matrix has an overall neutral charge in a pH range between the first and second pH. The pH depende nt ion exchange matrices of the present invention are designed to bind to the target nucleic acid at a pH wherein the overall charge of the matrix is positive, and to release the target nucleic acid as the pH of the surroundin g solution is increased. The target nucleic acid can be released from the pH dependent matrix in little or no salt and at about a neutral pH. The matrice s and methods of this invention enable one to isolate a target nucleic acid in very few steps, without the use of hazardous chemicals. Target nucleic acids isolated using the pH dependent ion exchange matrices according to the prese nt invention can be used immediately without further extraction or isolation.

5. 发明专利

AU2594202A Lysate clearance and nucleic acid isolation using silanized silica matrices 未知
公开(公告)号：AU2594202A
公开(公告)日：2002-05-21
申请号：AU2594202
申请日：2001-11-08
申请人： PROMEGA CORP
发明人： BITNER REX M , SIMPSON DANIEL J , FLEMMING RODERICK G , KOLLER SUSAN C
IPC分类号： G01N33/48 , B01D15/08 , B01D43/00 , B01J20/10 , B03C1/00 , C07B61/00 , C07H21/00 , C12N15/09 , C12N15/10 , C07H1/08

6. 发明专利

CA2372521A1 MIXED-BED SOLID PHASE AND ITS USE IN THE ISOLATION OF NUCLEIC ACIDS 未知
公开(公告)号：CA2372521A1
公开(公告)日：2000-11-23
申请号：CA2372521
申请日：2000-05-12
申请人： PROMEGA CORP
发明人： GROSCH JOSEPHINE C , SMITH CRAIG E , BITNER REX M , HOLMES DIANA L , SIMPSON DANIEL J , KATZHENDLER JEHOSHUA
IPC分类号： G01N33/53 , B01D15/04 , B01D15/08 , B01J41/20 , B01J47/04 , C12N15/09 , C12N15/10 , G01N33/553 , B01J41/06
摘要： Mixed-bed solid phases are provided, with methods for using such solid phase s to isolate target nucleic acids, such as plasmid DNA, chromosomal DNA, RNA, or nucleic acids generated by enzymatic amplification from contaminants, including proteins, lipids, cellular debris, or other nucleic acids. The mix ed- bed solid phases of this invention are mixtures of at least two different solid phases, each of which has a capacity to bind to the target nucleic aci d under different solution conditions, and the capacity to release the nucleic acid under similar elution conditions. By exchanging solution conditions according to the methods of this invention, one can remove contaminants from the target nucleic acid bound to the mixed-bed solid phase, then elute the target nucleic acid in an elution buffer.

7. 发明公开

EP1581337A4 COMPOSITION FOR SEPARATING MOLECULES 审中-公开
标题翻译：组成用于分离分子
公开(公告)号：EP1581337A4
公开(公告)日：2008-05-07
申请号：EP03777714
申请日：2003-10-20
申请人： PROMEGA CORP
发明人： SIMPSON DANIEL J , JOHNSON TONNY , FLEMMING RODERICK G
IPC分类号： B01J20/22 , B01J20/32 , B01J45/00 , C02F1/28 , C07K1/20 , C12N9/00
CPC分类号： B01D15/3828 , B01J20/103 , B01J20/28009 , B01J20/286 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3234 , B01J20/3242 , B01J20/3251 , B01J20/3259 , B01J20/3265 , B01J20/3289 , B01J20/3293 , B01J45/00 , B01J2220/58 , B01J2220/66 , C02F1/285 , C02F2101/20 , C07K1/20 , G01N2030/042
摘要： The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.

8. 发明专利

AU774810B2 Mixed-bed solid phase and its use in the isolation of nucleic acids 未知
公开(公告)号：AU774810B2
公开(公告)日：2004-07-08
申请号：AU4841500
申请日：2000-05-12
申请人： PROMEGA CORP
发明人： SMITH CRAIG E , HOLMES DIANA L , SIMPSON DANIEL J , KATZHENDLER JEHOSHUA , BITNER REX M , GROSCH JOSEPHINE C
IPC分类号： G01N33/53 , B01D15/04 , B01D15/08 , B01J41/20 , B01J47/04 , C12N15/09 , C12N15/10 , G01N33/553 , B01J41/06

9. 发明专利

AU772046B2 pH dependent ion exchange matrix and method of use in the isolation of nucleic acids 未知
公开(公告)号：AU772046B2
公开(公告)日：2004-04-08
申请号：AU5126100
申请日：2000-05-05
申请人： PROMEGA CORP
发明人： SMITH CRAIG E , HOLMES DIANA L , SIMPSON DANIEL J , KATZHENDLER JEHOSHUA , BITNER REX M , GROSCH JOSEPHINE C
IPC分类号： G01N30/00 , B01D15/04 , B01D15/08 , B01J20/10 , B01J20/22 , B01J20/286 , B01J39/26 , B01J41/20 , B01J43/00 , C07H21/00 , C12N15/09 , C12N15/10 , G01N30/88 , C07H1/00
摘要： Disclosed are pH dependent ion exchange matrices, methods of making, and methods of use.

10. 发明专利

AU2667397A Novel reagent, method, and kit for the quantitation of oxidation-reduction phenomena in proteins and peptides 未知
公开(公告)号：AU2667397A
公开(公告)日：1997-11-07
申请号：AU2667397
申请日：1997-04-14
申请人： PROMEGA CORP
发明人： SHULTZ JOHN W , SELMAN SUSANNE , SIMPSON DANIEL J
IPC分类号： C07D271/12 , C07D413/12 , C12Q1/26 , G01N31/22

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