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1. 发明申请

WO2015050627A1 PLANT-BASED RECOMBINANT BUTYRYLCHOLINESTERASE PRODUCTION METHODS 审中-公开
标题翻译：基于植物的重组丁基胆碱酯酶生产方法
公开(公告)号：WO2015050627A1
公开(公告)日：2015-04-09
申请号：PCT/US2014/049387
申请日：2014-08-01
申请人： POGUE, Greg , HIATT, Ernie , KANDZIA, Romy , WERNER, Stefan , THIEME, Frank , MOR, Tsafrir
发明人： POGUE, Greg , HIATT, Ernie , KANDZIA, Romy , WERNER, Stefan , THIEME, Frank , MOR, Tsafrir
IPC分类号： C12N9/00
CPC分类号： C12N9/18 , A61K38/465 , C12Y301/01008
摘要： A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.
摘要翻译：提供了一种用于生产重组丁酰胆碱酯酶（rBuChE）的新的，可靠的，易于扩展和重现的方法。通过利用植物转染方法，已经显示各种植物菌株在可接受的制造过程中产生有效和可缩放量的rBuChE，以允许可靠水平的这些酶用于所需的神经药物保护要求（包括四聚物产品）。同样，工程化植物品系中的这些方法已经显示出以四聚体形式合适地生产这些酶，其中聚糖形成和sialyalation（用于末端基团）以允许针对有机磷剂暴露的最佳效力以及在植物来源内的适当的免疫原性反应。因此，包括哺乳动物细胞内的转染和生产的总体生产方法以及从植物获得这种可靠的采购平台所涉及的工艺步骤。

2. 发明申请

WO2013040572A8 COMPOSITIONS AND METHODS FOR THE PRODUCTION AND USE OF HUMAN CHOLINESTERASES 审中-公开
公开(公告)号：WO2013040572A8
公开(公告)日：2013-03-21
申请号：PCT/US2012/055781
申请日：2012-09-17
申请人： ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY , MOR, Tsafrir , KANNAN, Latha , LARRIMORE, Katherine, E.
发明人： MOR, Tsafrir , KANNAN, Latha , LARRIMORE, Katherine, E.
IPC分类号： C12N15/83 , C12N15/29 , C12N15/55 , C12N9/16
摘要： In some aspects, the present invention relates to compositions and methods for the production of human cholinesterases. More particularly, it relates to methods for the production of human cholinesterases using transient expression and vectors for producing the same. In one aspect, the present invention relates to a plant viral vector encoding a plant codon-optimized DNA sequence that results in accumulation of the cholinesterase in a plant leaf at levels greater than 20 mg, and in some embodiments greater than 200 mg, of the enzyme per kilogram of the plant leaf.

3. 发明授权

EP3052618B1 PLANT-BASED RECOMBINANT BUTYRYLCHOLINESTERASE PRODUCTION METHODS 有权转让
公开(公告)号：EP3052618B1
公开(公告)日：2020-07-22
申请号：EP14850338.6
申请日：2014-08-01
申请人： Kentucky Bioprocessing, Inc. , Mor, Tsafrir
发明人： POGUE, Greg , HIATT, Ernie , KANDZIA, Romy , WERNER, Stefan , THIEME, Frank , MOR, Tsafrir , HUME, Steven
IPC分类号： C12N9/00 , A61K38/46 , C12N9/18

4. 发明申请

WO2009024957A1 MATERIALS AND METHODS FOR TREATING OR PREVENTING ORGANOPHOSPHATE EXPOSURE ASSOCIATED DAMAGE 审中-公开
标题翻译：用于治疗或预防有机磷酸盐暴露相关损害的材料和方法
公开(公告)号：WO2009024957A1
公开(公告)日：2009-02-26
申请号：PCT/IL2007/001032
申请日：2007-08-20
申请人： YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM , SOREQ, Hermona , MOR, Tsafrir
发明人： SOREQ, Hermona , MOR, Tsafrir
IPC分类号： A61K38/46 , A61K9/00 , A61P39/00
CPC分类号： A61K9/0019 , A61K9/0073 , A61K9/7007 , A61K38/465 , C12Y301/01007
摘要： A method of treating or preventing organophosphate exposure associated damage in a subject in need thereof is provided. The method comprising providing the subject with a therapeutically effective amount of the acetylcholinesterase splice variant AChE-R ("readthrough" AChE) to thereby. treat the organophosphate exposure associated damage in the subject.
摘要翻译：提供了在有需要的受试者中治疗或预防有机磷酸盐暴露相关损伤的方法。该方法包括向受试者提供治疗有效量的乙酰胆碱酯酶剪接变体AChE-R（“通过”AChE）“。治疗受试者中有机磷酸盐暴露相关的损伤。

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