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1. 发明申请

WO2017078836A1 METHODS AND COMPOSITIONS OF IMPROVED PLANT TRANSFORMATION 审中-公开
标题翻译：改进植物转化的方法和组合物
公开(公告)号：WO2017078836A1
公开(公告)日：2017-05-11
申请号：PCT/US2016/049132
申请日：2016-08-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ANAND, Ajith , BASS, Steven Henry , CHO, Hyeon-Je , KLEIN, Theodore Mitchell , LASSNER, Michael , MCBRIDE, Kevin E
IPC分类号： C12N15/82 , C07K14/195
CPC分类号： C12N15/8202 , C07K14/195 , C12N15/8205
摘要： The present disclosure provides methods and compositions for the vectors comprising vir genes. The present disclosure further provides a vector comprising: (a) an origin of replication for propagation and stable maintenance in Escherichia coli ; (b) an origin of replication for propagation and stable maintenance in Agrobacterium spp.; (c) a selectable marker gene; and (d) Rhizobiaceae virulence genes virB1-B11 or r-virB1-B11 , virC1-C2 or r-virC1-C2 , virD1-D2 or r-virD1-D2 , and virG or r-virG , or variants and derivatives thereof, wherein the vector comprising the virulence genes r-virB1-B11 , r-virC1-C2 , r-virD1-D2 , and r-virG further comprises a r-galls virulence gene, or variants and derivatives thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
摘要翻译：本公开提供了用于包含vir基因的载体的方法和组合物。本公开还提供了一种载体，其包含：（a）用于在大肠杆菌中繁殖和稳定维持的复制起点; （b）在土壤杆菌属中繁殖和稳定维持的复制起点。（c）选择标记基因; 和（d）根瘤菌科毒力基因virB1-B11或r-virB1-B11，virC1-C2或<！ - SIPO < ，virD1-D2或r-virD1-D2，和virG1或r2，或virG1或r2 -virG或其变体和衍生物，其中包含毒力基因rrvirB1-B11，rrvirC1-C2，rr -virD1-D2和r-virG进一步包含r-galls毒力基因或其变体和衍生物。该摘要旨在作为用于在特定技术中进行搜索的目的的扫描工具，并且不旨在限制本公开。

2. 发明申请

WO2019226553A1 MODIFICATION OF GLUTAMINE SYNTHETASE TO IMPROVE YIELD IN PLANTS 审中-公开
公开(公告)号：WO2019226553A1
公开(公告)日：2019-11-28
申请号：PCT/US2019/033130
申请日：2019-05-20
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： BASS, Steven Henry , BERMUDEZ, Ericka , HOU, Zhenglin , LAWIT, Shai , SANDOVAL, Marian Beecher , SHEN, Bo , THELEN, Peter , WANG, Yang , WILLIAMS, Robert W , WU, Jingrui , ZHANG, Jun
IPC分类号： A01H1/00 , A01H5/00 , A01H7/00 , C07H21/04 , C12N15/52
摘要： Provided are compositions comprising polynucleotides encoding glutamine synthetase (GS) polypeptides having improved properties, such as increased enzymatic activity and/or increased thermostability. Also provided are recombinant DNA constructs, plants, plant cells, seed, grain comprising the polynucleotides. Additionally, various methods of employing the polynucleotides in plants, such as methods for increasing GS activity in a plant, methods for increase seed protein content in a seed of a plant, and methods for increasing yield of a plant, are also provided herein.

3. 发明申请

WO2019169150A1 PLANT HEALTH ASSAY 审中-公开
公开(公告)号：WO2019169150A1
公开(公告)日：2019-09-06
申请号：PCT/US2019/020079
申请日：2019-02-28
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： BASS, Steven Henry , CHO, Hyeon-Je , CHO, Myeong-Je , CRANE, Virginia , HECKERT, Matthew J. , JIN, Jian , JONES, Todd J. , MCBRIDE, Kevin E. , SANDAHL, Jeanne , TIWARI, Shiv Bahadur
IPC分类号： G01N33/50 , C12Q1/6897 , C12N15/82
摘要： Methods of detecting the impacts on plant health attributable to the presence of one or more agronomically important polypeptides of interest in a transgenic plant are disclosed. The methods involve transforming plants or plant cells with nucleic acid sequences encoding proteins of agronomically important traits. The transformed plants or plant cells expressing the nucleic acid sequences encoding the proteins of agronomically important traits are compared to transformed plants or plant cells expressing a neutral control gene to detect the impacts on plant health attributable to the presence of the one or more agronomically important polypeptides of interest.

4. 发明申请

WO2017066164A1 METHODS AND COMPOSITIONS FOR TRANSFORMATION COMPRISING NEGATIVE SELECTION MARKERS 审中-公开
标题翻译：包含阴性选择标记的转化方法和组合物
公开(公告)号：WO2017066164A1
公开(公告)日：2017-04-20
申请号：PCT/US2016/056375
申请日：2016-10-11
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY
发明人： ANAND, Ajith , BASS, Steven Henry , GORDON-KAMM, William James , LOWE, Keith S. , SHARPE, Pamela L.
IPC分类号： C12N15/82 , C12N15/65 , C12N15/74
CPC分类号： C12N15/8205
摘要： The present disclosure comprises methods and compositions comprising a plant transforming bacterium of the Order Rhizobiales comprising conditional negative selectable marker genes.
摘要翻译：本发明包括包含包含条件阴性选择标记基因的根瘤菌属植物转化细菌的方法和组合物。

5. 发明申请

WO2020198496A1 MODIFIED AGROBACTERIUM STRAINS AND USE THEREOF FOR PLANT TRANSFORMATION 审中-公开
公开(公告)号：WO2020198496A1
公开(公告)日：2020-10-01
申请号：PCT/US2020/024993
申请日：2020-03-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ANNALURU, Sai Viswanath Jaya Prakash , BASS, Steven Henry
IPC分类号： C12N15/82 , C12R1/01
摘要： Modified Agrobacterium strains, methods of producing such modified Agrobacterium strains, and methods of using such modified Agrobacterium strains for producing transformed plants are disclosed herein.

6. 发明申请

WO2017040343A1 OCHROBACTRUM-MEDIATED TRANSFORMATION OF PLANTS 审中-公开
标题翻译：植物介导的植物转化
公开(公告)号：WO2017040343A1
公开(公告)日：2017-03-09
申请号：PCT/US2016/049135
申请日：2016-08-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY
发明人： ANAND, Ajith , BASS, Steven Henry , BERTAIN, Sean M. , CHO, Hyeon-Je , KINNEY, Anthony J , KLEIN, Theodore Mitchell , LASSNER, Michael , MCBRIDE, Kevin E , MOY, York , ROSEN, Barabara Ann Marie , WEI, Jun-Zhi
IPC分类号： C12N15/82 , C07K14/195
CPC分类号： C12N15/8202 , C07K14/195 , C12N15/8205
摘要： Methods and compositions for Ochrobactrum -mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.
摘要翻译：提供了Ochrobactrum介导的植物转化的方法和组合物。方法包括但不限于使用Ochrobactrum菌株将感兴趣的多核苷酸转移到植物细胞。这些包括依赖于VirD2的方法。组合物包括Ochrobactrum菌株，转移DNA，构建体和/或质粒。这些包括具有包含一个或多个毒力基因，边界区域和/或复制起点的质粒的Ochrobactrum菌株。还提供了通过该方法生产的包含感兴趣的多核苷酸的植物细胞，组织，植物和种子。

8. 发明公开

EP3947696A1 MODIFIED AGROBACTERIUM STRAINS AND USE THEREOF FOR PLANT TRANSFORMATION 审中-实审
公开(公告)号：EP3947696A1
公开(公告)日：2022-02-09
申请号：EP20719315.2
申请日：2020-03-26
申请人： Pioneer Hi-Bred International, Inc.
发明人： ANNALURU, Sai Viswanath Narayana , BASS, Steven Henry
IPC分类号： C12N15/82 , C12R1/01

9. 发明公开

EP3371313A1 METHODS AND COMPOSITIONS OF IMPROVED PLANT TRANSFORMATION 有权
公开(公告)号：EP3371313A1
公开(公告)日：2018-09-12
申请号：EP16763143.1
申请日：2016-08-26
申请人： Pioneer Hi-Bred International, Inc.
发明人： ANAND, Ajith , BASS, Steven Henry , CHO, Hyeon-Je , KLEIN, Theodore Mitchell , LASSNER, Michael , MCBRIDE, Kevin E
IPC分类号： C12N15/82 , C07K14/195
CPC分类号： C12N15/8202 , C07K14/195 , C12N15/8205
摘要： The present disclosure provides methods and compositions for the vectors comprising vir genes. The present disclosure further provides a vector comprising: (a) an origin of replication for propagation and stable maintenance in Escherichia coli; (b) an origin of replication for propagation and stable maintenance in Agrobacterium spp.; (c) a selectable marker gene; and (d) Rhizobiaceae virulence genes virB1-B11 or r-virB1-B11, virC1-C2 or r-virC1-C2, virD1-D2 or r-virD1-D2, and virG or r-virG, or variants and derivatives thereof, wherein the vector comprising the virulence genes r-virB1-B11, r-virC1-C2, r-virD1-D2, and r-virG further comprises a r-galls virulence gene, or variants and derivatives thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

10. 发明公开

EP3759489A1 PLANT HEALTH ASSAY 审中-公开
公开(公告)号：EP3759489A1
公开(公告)日：2021-01-06
申请号：EP19711478.8
申请日：2019-02-28
申请人： Pioneer Hi-Bred International, Inc.
发明人： BASS, Steven Henry , CHO, Hyeon-Je , CHO, Myeong-Je , CRANE, Virginia , HECKERT, Matthew J. , JIN, Jian , JONES, Todd J. , MCBRIDE, Kevin E. , SANDAHL, Jeanne , TIWARI, Shiv Bahadur
IPC分类号： G01N33/50 , C12Q1/6897 , C12N15/82

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