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1. 发明申请

WO2014150914A8 PHI-4 POLYPEPTIDES AND METHODS FOR THEIR USE 审中-公开
标题翻译： PHI-4多肽及其使用方法
公开(公告)号：WO2014150914A8
公开(公告)日：2015-11-26
申请号：PCT/US2014024524
申请日：2014-03-12
申请人： PIONEER HI BRED INT
发明人： CONG RUTH , HOU JINGTONG , HOU ZHENGLIN , PATTEN PHILLIP A , YAMAMOTO TAKASHI
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/195 , C07K2319/00 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译：提供了控制害虫的组成和方法。该方法涉及用编码杀虫蛋白的核酸序列转化生物体。特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。组合物是杀虫的核酸和细菌物种的蛋白质。这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，作为用于分离其他同源（或部分同源的）基因的探针。杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，dipteran，真菌，半翅目和线虫害虫种群，并用于生产具有杀虫活性的组合物。

2. 发明申请

WO2014150914A3 PHI-4 POLYPEPTIDES AND METHODS FOR THEIR USE 审中-公开
标题翻译： PHI-4多肽及其使用方法
公开(公告)号：WO2014150914A3
公开(公告)日：2014-11-13
申请号：PCT/US2014024524
申请日：2014-03-12
申请人： PIONEER HI BRED INT , CONG RUTH , HOU JINGTONG , HOU ZHENGLIN , PATTEN PHILLIP A , YAMAMOTO TAKASHI
发明人： CONG RUTH , HOU JINGTONG , HOU ZHENGLIN , PATTEN PHILLIP A , YAMAMOTO TAKASHI
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/195 , C07K2319/00 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译：提供了控制害虫的组成和方法。该方法涉及用编码杀虫蛋白的核酸序列转化生物体。特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。组合物是杀虫的核酸和细菌物种的蛋白质。这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，作为用于分离其他同源（或部分同源的）基因的探针。杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，dipteran，真菌，半翅目和线虫害虫群体，并用于生产具有杀虫活性的组合物。

3. 发明公开

EP2971000A4 PHI-4 POLYPEPTIDES AND METHODS FOR THEIR USE 审中-公开
标题翻译： PHI-4多肽及其使用方法
公开(公告)号：EP2971000A4
公开(公告)日：2016-11-23
申请号：EP14768504
申请日：2014-03-12
申请人： PIONEER HI BRED INT
发明人： CONG RUTH , HOU JINGTONG , HOU ZHENGLIN , PATTEN PHILLIP A , YAMAMOTO TAKASHI
IPC分类号： C12N15/82 , A01N63/02 , C07K14/195
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/195 , C07K2319/00 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

4. 发明专利

CA2826229A1 SYNTHETIC INSECTICIDAL PROTEINS ACTIVE AGAINST CORN ROOTWORM 未知
公开(公告)号：CA2826229A1
公开(公告)日：2012-08-16
申请号：CA2826229
申请日：2012-02-10
申请人： PIONEER HI BRED INT
发明人： BERMUDEZ ERICKA , CONG RUTH , HOU JINGTONG , YAMAMOTO TAKASHI
IPC分类号： C12N15/82 , C07K14/325
摘要： Traditionally, the primary method for impacting insect pest populations is the application of broad-spectrum chemical insecticides. However, there is increasing concern about the environmental hazards associated with the production and use of synthetic chemical pesticides. Thus, there is substantial interest in developing alternative pesticides, including biological control of insect pests of agricultural significance using a microbial agent or another species of insect. The present invention provides compositions and methods for such biological control. Modified Cry3 pesticidal polypeptides, polynucleotides encoding such polypeptides, and methods of use are disclosed. The modified polynucleotides provided herein can be used to transform organisms and cells of hosts comprising plant, insects, and microorganisms. The expression of modified polypeptides can provide the host with improved insecticidal activity against one or more insect pathogens.

5. 发明专利

CA2805803A1 COMPOSITIONS AND METHODS COMPRISING SEQUENCES HAVING HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) ACTIVITY 未知
公开(公告)号：CA2805803A1
公开(公告)日：2012-02-16
申请号：CA2805803
申请日：2011-08-12
申请人： PIONEER HI BRED INT
发明人： ALBERT HENRIK , BERMUDEZ ERICKA R , CASTLE LINDA A , DONG YUXIA , HECKERT MATTHEW J , HOU JINGTONG , HOU ZHENGLIN , LU JIAN , SIEHL DANIEL L , TAO YUMIN
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , C12N9/02 , C12Q1/68 , G01N33/573
摘要： Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.

6. 发明专利

CA2901316A1 PHI-4 POLYPEPTIDES AND METHODS FOR THEIR USE 未知
公开(公告)号：CA2901316A1
公开(公告)日：2014-09-25
申请号：CA2901316
申请日：2014-03-12
申请人： PIONEER HI BRED INT
发明人： CONG RUTH , HOU JINGTONG , HOU ZHENGLIN , PATTEN PHILLIP A , YAMAMOTO TAKASHI
IPC分类号： A01H5/00 , C12N15/82
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

7. 发明专利

ZA201505705B PHI-4 POLYPEPTIDES AND METHODS FOR THEIR USE 未知
公开(公告)号：ZA201505705B
公开(公告)日：2017-11-29
申请号：ZA201505705
申请日：2015-08-07
申请人： PIONEER HI-BRED INT INC
发明人： YAMAMOTO TAKASHI , PATTEN PHILLIP A , HOU ZHENGLIN , HOU JINGTONG , CONG RUTH
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

8. 发明专利

AU2015202867A1 COMPOSITIONS AND METHODS COMPRISING SEQUENCES HAVING HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) ACTIVITY 未知
公开(公告)号：AU2015202867A1
公开(公告)日：2015-06-18
申请号：AU2015202867
申请日：2015-05-26
申请人： PIONEER HI BRED INT
发明人： ALBERT HENRIK , BERMUDEZ ERICKA R , CASTLE LINDA A , DONG YUXIA , HECKERT MATTHEW J , HOU JINGTONG , HOU ZHENGLIN , LU JIAN , SIEHL DANIEL L , TAO YUMIN
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , C12N9/02 , C12Q1/68 , G01N33/573
摘要： OF DISCLOSURE Compositions and methods comprising polynucleotides and polypeptides having 4 hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.

9. 发明专利

AU2011289274A1 Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity 未知
公开(公告)号：AU2011289274A1
公开(公告)日：2013-01-31
申请号：AU2011289274
申请日：2011-08-12
申请人： PIONEER HI BRED INT
发明人： ALBERT HENRIK , BERMUDEZ ERICKA R , CASTLE LINDA A , DONG YUXIA , HECKERT MATTHEW J , HOU JINGTONG , HOU ZHENGLIN , LU JIAN , SIEHL DANIEL L , TAO YUMIN
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , C12N9/02 , C12Q1/68 , G01N33/573
摘要： Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.

10. 发明专利

MX2015013269A POLIPEPTIDOS PHI-4 Y METODOS PARA SUS USO. 未知
公开(公告)号：MX2015013269A
公开(公告)日：2016-06-07
申请号：MX2015013269
申请日：2014-03-12
申请人： PIONEER HI BRED INT
发明人： PATTEN PHILLIP A , CONG RUTH , HOU JINGTONG , HOU ZHENGLIN , YAMAMOTO TAKASHI
IPC分类号： A01N63/00 , C07K14/195 , C12N15/82
摘要： Se proporcionan composiciones y métodos para controlar plagas. Los métodos involucran transformar organismos con una secuencia de ácido nucleico que codifica una proteína insecticida. En particular, las secuencias de ácido nucleico son útiles para preparar plantas y microorganismos que poseen actividad insecticida. De esta manera, se proporcionan bacterias, plantas, células de planta, tejidos de planta y semillas transformadas. Las composiciones son ácidos nucleicos y proteínas insecticidas de especies bacterianas. Las secuencias encuentran uso en la construcción de vectores de expresión para transformación subsecuente en organismos de interés, como sondas para el aislamiento de otros genes homólogos (o parcialmente homólogos). Las proteínas insecticidas encuentran uso en controlar, inhibir el crecimiento o exterminar poblaciones de plagas de lepidópteros, coleópteros, dípteros, fúngicos, hemípteros y nemátodos y para producir composiciones con actividad insecticida.

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