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1. 发明申请

WO2011119739A1 METHOD AND MEDIUM FOR ACCELERATING TARGET ANALYTE GROWTH IN A TEST SAMPLE 审中-公开
标题翻译：在测试样品中加速目标分析生长的方法和介质
公开(公告)号：WO2011119739A1
公开(公告)日：2011-09-29
申请号：PCT/US2011/029635
申请日：2011-03-23
申请人： PILOTS POINT LLC , EDBERG, Stephen C.
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/14 , A21D8/04
CPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/14
摘要： A reagent mixture and testing procedure for accelerating mixing of the formulation with a specimen sample being tested by effervescing when hydrolized, thereby causing an internal "stirring" of the reagent and the specimen sample. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, environmental swabs, foodstuffs, and water, to mention a few.
摘要翻译：试剂混合物和试验方法，用于加速配制物与待测试样品的混合，待样品在水解时进行起泡，从而引起试剂和样品样品的内部“搅拌”。可以测试的样品样品的实例包括鼻拭子和病变拭子，环境拭子，食品和水等等。

2. 发明申请

WO2013070230A1 METHOD AND MEDIUM FOR DETECTING AN ANTIBIOTIC RESISTANT PATHOGENIC STAPHYLOCOCCI IN A TEST SAMPLE 审中-公开
标题翻译：在测试样品中检测抗生素耐药性图谱的方法和介质
公开(公告)号：WO2013070230A1
公开(公告)日：2013-05-16
申请号：PCT/US2011/060223
申请日：2011-11-10
申请人： PILOTS POINT LLC , EDBERG, Stephen C.
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/04 , C12Q1/14
CPC分类号： C12Q1/14 , C12Q1/56 , G01N2333/31 , G01N2333/952
摘要： A method and medium for detecting the presence or absence of a targeted strain of antibiotic resistant pathogenic staphylococci in a test sample is provided. The method includes the steps of: a) providing a test medium having at least one hydrolysable substrate, which substrate is operable to promote the growth of the targeted strain of antibiotic resistant pathogenic staphylococci and to produce a detectable signal when the hydrolyzable substrate is metabolized by the targeted strain of antibiotic resistant pathogenic staphylococci; b) forming a mixture of the test sample and hydrated test medium in the test tube; c) incubating the mixture at temperatures in the range of about 20°C to about 35°C; and d) detecting the presence or absence of the targeted strain of antibiotic resistant pathogenic staphylococci in the test sample by the presence or absence of the detectable signal in the mixture in the tube.
摘要翻译：提供了一种用于检测试验样品中抗生素抗性致病性葡萄球菌靶向菌株存在或不存在的方法和培养基。该方法包括以下步骤：a）提供具有至少一个可水解底物的测试介质，该底物可操作以促进抗生素抗性致病性葡萄球菌的靶向菌株的生长，并且当可水解底物代谢时产生可检测信号抗生素抗性致病性葡萄球菌的靶向菌株; b）在试管中形成试验样品和水合试验介质的混合物; c）在约20℃至约35℃的温度下温育混合物; 和d）通过管中混合物中存在或不存在可检测信号，检测测试样品中抗生素抗性致病性葡萄球菌靶向菌株的存在或不存在。

3. 发明申请

WO2011139285A1 METHOD AND MEDIUM FOR DETECTING THE PRESENCE OR ABSENCE OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN A TEST SAMPLE 审中-公开
标题翻译：用于检测甲硫氨酸耐药斑马鱼（MRSA）在试验样品中的存在或不存在的方法和培养基
公开(公告)号：WO2011139285A1
公开(公告)日：2011-11-10
申请号：PCT/US2010/038821
申请日：2010-06-16
申请人： PILOTS POINT LLC , EDBERG, Stephen C.
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/04 , C12Q1/14
CPC分类号： C12Q1/045 , C12Q1/14
摘要： A dry sample analyzing mixture, a liquid sample analyzing medium, and a sample analyzing method, are described for use in detecting the presence or absence of Methicillin Resistant Staphylococcus Aureus ("MRSA") in an incubated first generation biological or environmental specimen sample. First generation specimen samples that can be analyzed include nasal swabs, lesion swabs, skin swabs, throat swabs, food swabs, tanning salon swabs, gym swabs, restaurant swabs, and the like. The medium and method include an anti-ribosomal antibiotic component that will selectively prevent Methicillin Susceptible Staphylococcus Aureus ("MSSA") from growing in the medium, while allowing MRSA to grow in the medium. The medium also includes components which will stimulate growth of MRSA. The medium also includes components which will produce a detectable signal, which signal indicates the presence of MRSA in the incubated sample.
摘要翻译：描述了干样品分析混合物，液体样品分析介质和样品分析方法，用于检测在孵育的第一代生物或环境标本样品中是否存在耐甲氧西林金黄色葡萄球菌（“MRSA”）。可以分析的第一代标本样品包括鼻拭子，病变拭子，皮肤拭子，咽喉拭子，食物拭子，晒黑沙龙拭子，健身器皿，餐巾拭子等。培养基和方法包括抗核糖体抗生素成分，其将选择性地防止甲氧西林易感性金黄色葡萄球菌（“MSSA”）在培养基中生长，同时允许MRSA在培养基中生长。该培养基还包括会刺激MRSA生长的成分。培养基还包括将产生可检测信号的组分，该信号表明在培养的样品中存在MRSA。

4. 发明申请

WO2010151772A1 METHOD AND REAGENTS FOR DETECTING THE PRESENCE OR ABSENCE OF A TARGET SUBSTANCE IN A TEST SAMPLE 审中-公开
标题翻译：检测目标物质在测试样品中的存在或不存在的方法和试剂
公开(公告)号：WO2010151772A1
公开(公告)日：2010-12-29
申请号：PCT/US2010/040001
申请日：2010-06-25
申请人： PILOTS POINT LLC , EDBERG, Stephen C.
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/045 , C12Q1/04 , C12Q1/10 , C12Q1/14 , G01N2333/415
摘要： Reagent mixtures and testing procedures for detecting the presence or absence of various target substances such as aureus; vancomycin-resistant enterrococcus; Group B Streptococcus; E. coli; fungi; and others, involve the use of a testing reagents which include growth factors for the particular target substance, a detectable signal generator which is sensitive to the presence of the target substance, and hormonal compounds which will accelerate the growth of the target substance within a specimen being tested. Examples of specimen samples that can be tested include nasal swabs, throat swabs, lesion swabs, environmental swabs, foodstuffs, and water, to mention a few.
摘要翻译：用于检测各种目标物质如金黄色葡萄球菌存在或不存在的试剂混合物和检测程序; 耐万古霉素肠球菌; B组链球菌大肠杆菌; 菌类; 涉及使用包括特定目标物质的生长因子的测试试剂，对目标物质的存在敏感的可检测信号发生器，以及加速样品中目标物质生长的激素化合物被测试。可以测试的标本样品的实例包括鼻拭子，咽拭子，病变拭子，环境拭子，食品和水等等。

5. 发明申请

WO2010151764A1 METHOD AND REAGENTS FOR DETECTING THE PRESENCE OR ABSENCE OF STAPHYLOCOCCUS AUREUS IN A TEST SAMPLE 审中-公开
标题翻译：检测测试样品中存在或不存在白花菜的方法和试剂
公开(公告)号：WO2010151764A1
公开(公告)日：2010-12-29
申请号：PCT/US2010/039987
申请日：2010-06-25
申请人： PILOTS POINT LLC , EDBERG, Stephen C.
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/14
CPC分类号： C12Q1/14
摘要： A presence/absence test for Staphylococcus aureus (S. aureus) involves placing a first generation test sample in a solution that will clot in the presence of S. aureus. The solution contains components that will selectively grow S. aureus and also contains clotting factors that will react with S. aureus, if S. aureus is present in the sample, to clot the solution. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, among others. The test can also be modified to detect the presence or absence of methicillin resistant S. Aureus (MRSA). The addition of micro particles having a size in the range of about 0.1 micron to about 1.0 mm provides localities where the bacteria agglomerate, thereby significantly decreasing the clotting time, and providing a significantly stronger clot. The micro particles can be used in other bacteria tests to accelerate the production of an end result. Such other tests can include a vancomycin-resistant enterococcus test; a Group B Streptococcus test; a test for hemolytic E. coli; and a test for Listeria monocytogenes, to name a few. These tests are all performed in a liquid broth-type reagent mixture and do not necessarily involve clotting of the broth.
摘要翻译：金黄色葡萄球菌（金黄色葡萄球菌）的存在/不存在测试涉及将第一代测试样品置于在金黄色葡萄球菌存在下凝结的溶液中。该溶液含有选择性生长金黄色葡萄球菌的成分，并且还含有凝血因子，如果金黄色葡萄球菌存在于样品中，则会与金黄色葡萄球菌发生反应，以凝固溶液。可以测试的标本样品的实例包括鼻拭子和病变拭子等。也可以修改测试以检测耐甲氧西林金黄色葡萄球菌（MRSA）的存在或不存在。加入尺寸在约0.1微米至约1.0毫米范围内的微粒提供了细菌聚集的地方，从而显着降低凝固时间，并提供明显更强的凝块。微粒可用于其他细菌测试，以加速最终结果的产生。这样的其他测试可以包括耐万古霉素肠球菌测试; B组链球菌试验; 溶血性大肠杆菌检测; 和单核细胞增多性李斯特氏菌的检测，仅举几例。这些测试都是在液体肉汤型试剂混合物中进行的，并不一定涉及肉汤的凝结。

6. 发明申请

WO2014201464A1 METHODS FOR DETECTING THE PRESENCE OF MICROBES 审中-公开
标题翻译：检测MICROBES存在的方法
公开(公告)号：WO2014201464A1
公开(公告)日：2014-12-18
申请号：PCT/US2014/042539
申请日：2014-06-16
申请人： PILOTS POINT LLC
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/04 , C12M1/36 , G01N21/31
CPC分类号： C12Q1/06 , C12Q1/04 , G01N21/251 , G01N21/253 , G01N21/49 , G01N21/51 , G01N21/6486 , G01N35/00871 , G01N35/025 , G01N35/04 , G01N2035/00891 , G01N2035/0401 , G01N2201/061
摘要： A method and instrument for detecting the presence or absence of a target organism in a test sample is provided. The instrument includes a carousel, a light source, alight detector, and a control system. The carousel is configured to hold a plurality of test sample containers, with each container held by the carousel at a container position. Each of the containers holds an admixture of a test mixture inoculated with the test sample. The light detector is operable to detect light emitted by the light source that has passed through the test sample disposed within the test container. The control system includes a processor. The control system is adapted to produce information indicative of the presence or absence of the target organism in the test sample using signals produced by the light detector.
摘要翻译：提供了用于检测测试样品中目标生物体存在或不存在的方法和仪器。该仪器包括转盘，光源，检测器和控制系统。转盘被构造成容纳多个测试样本容器，每个容器由转盘保持在容器位置。每个容器保持接种测试样品的测试混合物的混合物。光检测器可操作以检测已经通过设置在测试容器内的测试样品的光源发射的光。控制系统包括处理器。控制系统适于使用由光检测器产生的信号产生指示测试样品中目标生物存在或不存在的信息。

7. 发明授权

EP2245179B1 METHOD AND MEDIUM FOR DETECTING THE PRESENCE OR ABSENCE OF STAPHYLOCOCCUS AUREUS IN A TEST SAMPLE 有权
标题翻译：方法和介质用于识别测试样品存在或不金黄色葡萄球菌
公开(公告)号：EP2245179B1
公开(公告)日：2013-07-24
申请号：EP09748868.8
申请日：2009-01-23
申请人： Pilots Point LLC
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/14
CPC分类号： C12Q1/14

8. 发明公开

EP3008202A1 METHODS FOR DETECTING THE PRESENCE OF MICROBES 审中-公开
标题翻译： VERFAHREN ZUM NACHWEIS VON MIKROORGANISMEN
公开(公告)号：EP3008202A1
公开(公告)日：2016-04-20
申请号：EP14811432.5
申请日：2014-06-16
申请人： Pilots Point LLC
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/04 , C12M1/36 , G01N21/31
CPC分类号： C12Q1/06 , C12Q1/04 , G01N21/251 , G01N21/253 , G01N21/49 , G01N21/51 , G01N21/6486 , G01N35/00871 , G01N35/025 , G01N35/04 , G01N2035/00891 , G01N2035/0401 , G01N2201/061
摘要： A method and instrument for detecting the presence or absence of a target organism in a test sample is provided. The instrument includes a carousel, a light source, alight detector, and a control system. The carousel is configured to hold a plurality of test sample containers, with each container held by the carousel at a container position. Each of the containers holds an admixture of a test mixture inoculated with the test sample. The light detector is operable to detect light emitted by the light source that has passed through the test sample disposed within the test container. The control system includes a processor. The control system is adapted to produce information indicative of the presence or absence of the target organism in the test sample using signals produced by the light detector.
摘要翻译：提供了用于检测测试样品中目标生物体存在或不存在的方法和仪器。仪器包括转盘，光源，检测器和控制系统。转盘被配置为容纳多个测试样本容器，每个容器由转盘保持在容器位置。每个容器保持与测试样品接种的测试混合物的混合物。光检测器可操作以检测已经通过设置在测试容器内的测试样品的光源发射的光。控制系统包括处理器。控制系统适于使用由光检测器产生的信号产生指示测试样品中目标生物体存在或不存在的信息。

9. 发明公开

EP2776572A1 METHOD AND MEDIUM FOR DETECTING AN ANTIBIOTIC RESISTANT PATHOGENIC STAPHYLOCOCCI IN A TEST SAMPLE 审中-公开
标题翻译：方法和介质用于识别致病性葡萄球菌的抗生素在测试样品
公开(公告)号：EP2776572A1
公开(公告)日：2014-09-17
申请号：EP11875504.0
申请日：2011-11-10
申请人： Pilots Point LLC
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/04 , C12Q1/14
CPC分类号： C12Q1/14 , C12Q1/56 , G01N2333/31 , G01N2333/952
摘要： A method and medium for detecting the presence or absence of a targeted strain of antibiotic resistant pathogenic staphylococci in a test sample is provided. The method includes the steps of: a) providing a test medium having at least one hydrolysable substrate, which substrate is operable to promote the growth of the targeted strain of antibiotic resistant pathogenic staphylococci and to produce a detectable signal when the hydrolyzable substrate is metabolized by the targeted strain of antibiotic resistant pathogenic staphylococci; b) forming a mixture of the test sample and hydrated test medium in the test tube; c) incubating the mixture at temperatures in the range of about 20°C to about 35°C; and d) detecting the presence or absence of the targeted strain of antibiotic resistant pathogenic staphylococci in the test sample by the presence or absence of the detectable signal in the mixture in the tube.

10. 发明公开

EP2245179A2 METHOD AND MEDIUM FOR DETECTING THE PRESENCE OR ABSENCE OF STAPHYLOCOCCUS AUREUS IN A TEST SAMPLE 有权
标题翻译：方法和介质用于识别测试样品存在或不金黄色葡萄球菌
公开(公告)号：EP2245179A2
公开(公告)日：2010-11-03
申请号：EP09748868.8
申请日：2009-01-23
申请人： Pilots Point LLC
发明人： EDBERG, Stephen C.
IPC分类号： C12Q1/14
CPC分类号： C12Q1/14
摘要： A presence/absence test for Staphylococcus aureus (S. aureus) involves placing a first generation test sample in a solution that will clot in the presence of S. aureus. The solution contains components that will selectively grow S. aureus and also contains clotting factors that will react with S. aureus, if S. aureus is present in the sample, to clot the solution. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, among others. The test can also be modified to detect the presence or absence of methicillin resistant S. Aureus (MRSA).

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