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2. 发明申请

US20170067092A1 MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS 审中-公开
标题翻译：使用PNA探针的熔融曲线分析，使用熔融曲线分析分析核苷酸多态性的方法和工具包
公开(公告)号：US20170067092A1
公开(公告)日：2017-03-09
申请号：US15300771
申请日：2014-04-04
申请人： PANAGENE INC.
发明人： Goon Ho Joe , Sung-Kee Kim , Heekyung Park , Chwang Siek Park , Se Ryun Kim , Yongtae Kim , Su Nam Kim
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2525/107 , C12Q2527/107 , C12Q2563/173
摘要： Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.
摘要翻译：提供了用于检测靶基因的核苷酸多态性的PNA探针，使用该靶基因的靶基因的核苷酸多态性的检测的解链曲线分析方法，包含解链曲线分析法的靶基因的核苷酸多态性分析方法，以及用于检测含有PNA探针的靶基因的核苷酸多态性的试剂盒。其特征在于根据本发明的PNA探针含有负电荷分子。根据本发明的修饰的PNA探针含有相对于目标DNA具有高识别能力的负电荷分子和与目标DNA的高偶联能力并且通过热快速解离，使得核苷酸多态性分析可以即使在显示两个解链曲线图的杂合样品中也可以相对容易地进行，并且可以同时分析两个或更多个相邻的单核苷酸多态性。

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