专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2009073201A3 ALTERNATE LABELING STRATEGIES FOR SINGLE MOLECULE SEQUENCING 审中-公开
公开(公告)号：WO2009073201A3
公开(公告)日：2009-06-11
申请号：PCT/US2008/013363
申请日：2008-12-03
申请人： PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , KORLACH, Jonas , ROITMAN, Daniel , EID, John , OTTO, Geoff , HARDENBOL, Paul , FLUSBERG, Benjamin
发明人： KORLACH, Jonas , ROITMAN, Daniel , EID, John , OTTO, Geoff , HARDENBOL, Paul , FLUSBERG, Benjamin
IPC分类号： G01N33/573 , G01N33/53 , G01N33/535
摘要： Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.

2. 发明授权

EP2217928B1 ALTERNATE LABELING STRATEGIES FOR SINGLE MOLECULE SEQUENCING 有权转让
标题翻译：单分子测序的替代标记策略
公开(公告)号：EP2217928B1
公开(公告)日：2017-08-09
申请号：EP08856043.8
申请日：2008-12-03
申请人： Pacific Biosciences of California, Inc.
发明人： KORLACH, Jonas , ROITMAN, Daniel , EID, John , OTTO, Geoff , HARDENBOL, Paul , FLUSBERG, Benjamin
IPC分类号： G01N33/573 , G01N33/53 , G01N33/535 , G01N33/542 , G01N33/58 , B01L3/00 , C12Q1/68 , G01N33/543 , C08G83/00
CPC分类号： G01N33/54373 , C12Q1/6874 , G01N33/542 , G01N33/54313 , G01N33/573 , G01N33/58 , G01N33/585 , C12Q2565/607 , C12Q2565/531 , C12Q2563/143 , C12Q2563/137

3. 发明公开

EP2217928A2 ALTERNATE LABELING STRATEGIES FOR SINGLE MOLECULE SEQUENCING 有权转让
标题翻译：备选MARK程序单分子测序
公开(公告)号：EP2217928A2
公开(公告)日：2010-08-18
申请号：EP08856043.8
申请日：2008-12-03
申请人： Pacific Biosciences of California, Inc.
发明人： KORLACH, Jonas , ROITMAN, Daniel , EID, John , OTTO, Geoff , HARDENBOL, Paul , FLUSBERG, Benjamin
IPC分类号： G01N33/573 , G01N33/53 , G01N33/535
CPC分类号： G01N33/54373 , C12Q1/6874 , G01N33/542 , G01N33/54313 , G01N33/573 , G01N33/58 , G01N33/585 , C12Q2565/607 , C12Q2565/531 , C12Q2563/143 , C12Q2563/137
摘要： Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.

4. 发明申请

WO2005029040A3 SYSTEM AND METHODS FOR ENHANCING SIGNAL-TO-NOISE RATIOS OF MICROARRAY-BASED MEASUREMENTS 审中-公开
公开(公告)号：WO2005029040A3
公开(公告)日：2005-03-31
申请号：PCT/US2004/030768
申请日：2004-09-17
申请人： PARALLELE BIOSCIENCES, INC. , NAMSARAEV, Eugeni , KARLIN-NEUMANN, George , FAHAM, Malek , JAIN, Maneesh , HARDENBOL, Paul , WILLIS, Thomas, D. , WANG, Zhiyong
发明人： NAMSARAEV, Eugeni , KARLIN-NEUMANN, George , FAHAM, Malek , JAIN, Maneesh , HARDENBOL, Paul , WILLIS, Thomas, D. , WANG, Zhiyong
IPC分类号： G01N21/76 , G01N33/53
摘要： The present invention provides systems and methods for large-scale genetic measurements by generating from a sample labeled target sequences whose length, orientation, label, and degree of overlap and complementarity are tailored to corresponding end-attached probes of a solid support so that signal-to-noise ratios of measurement from specifically hybridized labeled target sequences are maximized. Systems for implementing methods of the invention include a set of sample-interacting probes to produce amplicons that either each contain a segment of a target polynucleotide or an oligonucleotide tag that corresponds to a segment of a target polynucleotide, one or more solid phase supports that contain a plurality of end-attached probes, and methods of generating from sample-interacting probe amplicons from which labeled target sequences are tailored for hybridization to the solid phase supports, such as microarrays. In one aspect, labeled target sequences and end-attached probe of the solid phase supports comprise oligonucleotide tags and tag complements, respectively, selected from a minimally cross-hybridizing set.

5. 发明申请

WO2006014869A1 SIMULTANEOUS ANALYSIS OF MULTIPLE GENOMES 审中-公开
标题翻译：多发基因的同时分析
公开(公告)号：WO2006014869A1
公开(公告)日：2006-02-09
申请号：PCT/US2005/026313
申请日：2005-07-25
申请人： PARALLELE BIOSCIENCE, INC. , HARDENBOL, Paul , KARLIN-NEUMANN, George , WILLIS, Thomas, D. , JAIN, Maneesh , NACLERIO, Nicholas
发明人： HARDENBOL, Paul , KARLIN-NEUMANN, George , WILLIS, Thomas, D. , JAIN, Maneesh , NACLERIO, Nicholas
IPC分类号： C12Q1/68 , G06F19/00
CPC分类号： C12Q1/6837 , C12Q1/6858 , C12Q2537/143 , C12Q2525/161 , C12Q2533/107
摘要： The invention provides methods for multiplexing readouts from multiple hybridization-based assays that each comprise one or more hybridization or annealing steps and one or more enzymatic processing steps. In one aspect, the invention permits simultaneously analysis of a plurality of genomes by separately hybridizing a set of probes with the different genomes to form sets of probe-genome complexes in separate reaction mixtures that are combined and enzymatically treated to form amplifiable probes. From such amplifiable probes, labeled probes are produced so that for each different locus of each different genome there is a unique labeled probe, which are then specifically hybridized to their respective complements on a microarray. In another aspect, labeled oligonucleotide tags are produced from amplifiable probes. The invention is useful in applications of multiplexed hybridization-based assays for measuring characteristics of genomic samples taken from many different individuals. By conducting hybridization steps separately on samples different individuals then combining them for enzymatic processing, one takes advantage of natural reaction rate differences between hybridization reactions and enzymatic reactions to enable analysis of products of multiple assays on a single readout platform.
摘要翻译：本发明提供了用于从多个基于杂交的测定中复用读出的方法，每个测序包含一个或多个杂交或退火步骤和一个或多个酶处理步骤。一方面，本发明允许通过将一组探针与不同基因组分开杂交来同时分析多个基因组，以在组合和酶处理以形成可扩增探针的分开的反应混合物中形成探针 - 基因组复合物组。从这样的可扩增探针中产生标记的探针，使得对于每个不同基因组的每个不同基因座，存在唯一标记的探针，然后在微阵列上与其各自的互补序列特异性杂交。另一方面，标记的寡核苷酸标签由可扩增的探针产生。本发明在多基于杂交的测定用于测量从许多不同个体获取的基因组样品的特征的应用中是有用的。通过分别对样品进行杂交步骤，然后将它们组合用于酶处理，一个利用杂交反应和酶反应之间的天然反应速率差异，以使得能够在单个读出平台上分析多个测定的产物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式