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    • 5. 发明授权
    • Methods of making an RNP particle having nucleotide integrase activity
    • 制备具有核苷酸整合酶活性的RNP颗粒的方法
    • US6001608A
    • 1999-12-14
    • US85603
    • 1998-05-27
    • Alan M. LambowitzGeorg MohrRoland SaldanhaManabu MatsuuraClifford James BeallJiam YangSteven ZimmerlyHuatao Guo
    • Alan M. LambowitzGeorg MohrRoland SaldanhaManabu MatsuuraClifford James BeallJiam YangSteven ZimmerlyHuatao Guo
    • C12N9/22C12P19/34C07H21/04C12N9/12C12P21/06
    • C12N9/22
    • Methods for preparing nucleotide integrases are provided. The nucleotide integrases are prepared by combining in vitro an excised, group II intron RNA, referred to hereinafter as "exogenous RNA", with a group II intron-encoded protein. The exogenous RNA is prepared by in vitro transcription of a DNA molecule which comprises a group II intron sequence. In one embodiment, the group II intron-encoded protein is made by introducing into a host cell a DNA molecule that comprises at least the open reading frame sequence of a group II intron and then expressing the open reading frame sequence in the host cell. The DNA molecule may comprise the open reading frame sequence operably linked to a promoter, preferably an inducible promoter. Thereafter, the cell is fractionated and the protein is recovered and combined in vitro with the exogenous RNA to provide RNP particles having nucleotide integrase activity. In another embodiment, the DNA molecule comprise a group II intron sequence that encodes both a group II intron RNA as well as a group II intron encoded protein. The DNA molecule is then expressed in the host cell to provide RNP particles that comprise the group II intron-encoded protein bound to the group II intron RNA. Thereafter, the RNP particles comprising the group II intron-encoded protein and the group II intron RNA are isolated from the cell and treated with a nuclease to remove the RNA and to provide the group II-intron encoded protein. The group II intron-encoded protein is then combined in vitro with the exogenous RNA to provide RNP particles having nucleotide integrase activity.
    • 提供了制备核苷酸整合酶的方法。 核苷酸整合是通过将体外切割的II组内含子RNA(以下称为“外源RNA”)与II组内含子编码的蛋白质组合来制备的。 通过体外转录包含II组内含子序列的DNA分子制备外源RNA。 在一个实施方案中,II组内含子编码的蛋白质是通过向宿主细胞中引入至少包含第II组内含子的开放阅读框序列,然后在宿主细胞中表达开放阅读框序列的DNA分子来制备的。 DNA分子可以包含可操作地连接到启动子,优选诱导型启动子的开放阅读框序列。 此后,分离细胞并回收蛋白质并将其与外源RNA组合以提供具有核苷酸整合酶活性的RNP颗粒。 在另一个实施方案中,DNA分子包含编码II组内含子RNA以及II组内含子编码蛋白质的II组内含子序列。 然后将DNA分子在宿主细胞中表达以提供包含与II组内含子RNA结合的II组内含子编码的蛋白质的RNP颗粒。 此后,从细胞中分离包含II组内含子编码的蛋白质和II组内含子RNA的RNP颗粒,并用核酸酶处理以除去RNA并提供II组内含子编码的蛋白质。 然后将II组内含子编码的蛋白质在体外与外源RNA组合以提供具有核苷酸整合酶活性的RNP颗粒。
    • 7. 发明授权
    • Liquid crystal display device and driving method thereof
    • 液晶显示装置及其驱动方法
    • US06256004B1
    • 2001-07-03
    • US08984084
    • 1997-12-03
    • Yoshihiro IzumiTokihiko ShinomiyaManabu Matsuura
    • Yoshihiro IzumiTokihiko ShinomiyaManabu Matsuura
    • G09G336
    • G09G3/3607G09G3/3648G09G2300/026
    • A large-area liquid crystal display device of the present invention is fabricated by arranging liquid crystal panels to form a plane surface and connecting the panels at their edges parallel to a first direction corresponding to an up-and-down direction of a displayed image to be obtained. Each liquid crystal panel has pixels which are arranged in a matrix form and include color filters and pixel electrodes arranged in the first direction to correspond to the color filters, scanning lines arranged in the first direction, and signal lines arranged in a second direction intersecting the first direction. When driving the liquid crystal display device, the arrangement of image data for performing line sequential scanning in the first direction is changed into an arrangement for performing line sequential scanning in the second direction using a memory. This structure realizes a large-area liquid crystal display device capable of providing a high-quality displayed image with a less noticeable joint of the liquid crystal panels.
    • 本发明的大面积液晶显示装置是通过将液晶面板排列成平面而构成的,并且在平行于与显示图像的上下方向对应的第一方向的边缘上连接面板, 得到。 每个液晶面板具有以矩阵形式布置的像素,并且包括沿第一方向排列的滤色器和像素电极,以对应于滤色器,沿第一方向布置的扫描线,以及沿与第二方向相交的第二方向布置的信号线 第一个方向 当驱动液晶显示装置时,用于在第一方向上执行行顺序扫描的图像数据的布置被改变为用于使用存储器在第二方向上执行行顺序扫描的布置。 该结构实现了能够提供具有液晶面板不太明显的接合的高质量显示图像的大面积液晶显示装置。
    • 8. 发明授权
    • Methods for preparing nucleotide integrases
    • 核苷酸整合酶的制备方法
    • US5804418A
    • 1998-09-08
    • US752238
    • 1996-11-19
    • Alan Marc LambowitzGeorg MohrRoland SaldanhaManabu Matsuura
    • Alan Marc LambowitzGeorg MohrRoland SaldanhaManabu Matsuura
    • C12N9/22C12P21/02C12N9/12C12N15/54C12P19/34
    • C12N9/22
    • The present invention provides new, improved, and easily manipulable methods for making nucleotide integrases. In one embodiment, the nucleotide integrase is prepared by introducing a DNA molecule which comprises a group II intron DNA sequence into a host cell. The group II intron DNA sequence is then expressed in the host cell such that RNP particles having nucleotide integrase activity are formed in the cell. Such RNP particles comprise an exiced group II intron RNA encoded by the introduced DNA molecule and a group II intron-encoded protein encoded by the introduced DNA molecule. Thereafter, the nucleotide integrase is isolated from the cell. In another embodiment, the nucleotide integrase is prepared by combining in vitro an excised, group II intron RNA, referred to hereinafter as "exogenous RNA", with a group II intron-encoded protein. In another embodiment, the nucleotide integrase is prepared by combining in vitro an excised, group II intron RNA, referred to hereinafter as "exogenous RNA", with an RNA-protein complex which comprises a group II intron-encoded protein. Preferably, the exogenous RNA is prepared by in vitro transcription of a DNA molecule which comprises the group II intron sequence. Preferably, the group II intron-encoded protein is made by introducing into a host cell a DNA molecule which comprises the open reading frame sequence of a group II intron, and then expressing the the open reading sequence in the host cell such that the group II intron-encoded protein encoded by the open reading frame sequence is formed in the cell. Preferably, the RNA-protein complex is made by introducing into a host cell a DNA molecule comprising a group II intron DNA sequence which encodes a splicing-defective group II intron RNA. The present invention also relates to a nucleotide integrase and an improved method for making RNA-protein complexes for use in preparing nucleotide integrases in vitro.
    • 本发明提供了用于制备核苷酸整合酶的新的,改进的和易于操作的方法。 在一个实施方案中,通过将包含II组内含子DNA序列的DNA分子引入宿主细胞来制备核苷酸整合酶。 然后在宿主细胞中表达第II组内含子DNA序列,使得在细胞中形成具有核苷酸整合酶活性的RNP颗粒。 这样的RNP颗粒包含由导入的DNA分子编码的经过II组的内含子核糖核酸和由引入的DNA分子编码的II组内含子编码的蛋白质。 此后,从细胞中分离出核苷酸整合酶。 在另一个实施方案中,核苷酸整合酶通过将体外切割的II组内含子RNA(以下称为“外源RNA”)与II组内含子编码的蛋白质组合来制备。 在另一个实施方案中,核苷酸整合酶通过将体外包含第II组内含子编码蛋白质的RNA-蛋白质复合物的切割的II组内含子RNA(以下称为“外源RNA”)组合而制备。 优选地,外源RNA通过体外转录包含II组内含子序列的DNA分子来制备。 优选地,II组内含子编码的蛋白质通过将包含第II组内含子的开放阅读框序列的DNA分子引入宿主细胞,然后在宿主细胞中表达开放阅读序列使得组II 由开放阅读框序列编码的内含子编码的蛋白质在细胞中形成。 优选地,通过将​​包含编码剪接缺陷型II组内含子RNA的II组内含子DNA序列的DNA分子导入宿主细胞来制备RNA-蛋白质复合物。 本发明还涉及核苷酸整合酶和用于制备用于在体外制备核苷酸整合酶的RNA-蛋白复合物的改进方法。