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1. 发明授权

US5006309A Immunoassay device with liquid transfer between wells by washing 失效
标题翻译：免疫测定装置，通过洗涤在井间进行液体转移
公开(公告)号：US5006309A
公开(公告)日：1991-04-09
申请号：US184726
申请日：1988-04-22
申请人： Omar S. Khalil , Charles F. Hanna , Thomas F. Zurek , Denise A. Grigalauski
发明人： Omar S. Khalil , Charles F. Hanna , Thomas F. Zurek , Denise A. Grigalauski
IPC分类号： B01L3/00 , G01N33/543
CPC分类号： G01N33/54366 , B01L3/5023 , B01L2200/027 , B01L2300/0809 , B01L2400/0406 , B01L2400/049 , Y10T436/255 , Y10T436/2575
摘要： A disposable device suitable for performing automated solid-state diagnostic assays which employs microparticles to complex an analyte and where the microparticle complex becomes retained and immobilized on a fibrous matrix such that the presence of analyte on the microparticles can be detected by an optical device. A device is disclosed having a shallow sample well for receiving a sample and reagents for forming a reaction mixture, a read well having (a) an entrance port and wash receiving structure for receiving a quantity of sample and assay reagents, (b) a fibrous matrix for retaining and immobilizing microparticle/analyte complexes for detection, the fibrous matrix positioned below the wash receiving structure, and having an average spatial separation of fibers greater than the average diameter of the microparticles and (c) a structure below the fibrous matrix for assisting the flow of sample and assay reagents through the fibrous matrix, such as an absorbent material or vacuum below the fibrous matrix and a passage means communicating between the shallow sample well and the read well where the sample and reaction mixtures can be transferred and washed from the shallow sample well into the read well without being contacted by any apparatus.
摘要翻译：一种适用于执行自动化固态诊断测定的一次性装置，其使用微粒来复合分析物，并且其中微粒复合物保留并固定在纤维基质上，使得微粒上的分析物的存在可以通过光学装置检测。公开了一种装置，其具有用于接收样品的浅样品阱和用于形成反应混合物的试剂，读数井具有（a）用于接收一定量的样品和测定试剂的入口和洗涤接收结构，（b）纤维用于保持和固定用于检测的微粒/分析物复合物的基质，所述纤维基质位于洗涤接收结构下方，并且具有大于微粒平均直径的纤维的平均空间分离，和（c）纤维基质下面的结构用于辅助通过纤维基质（例如吸收材料或纤维基质下方的真空）的样品和测定试剂的流动以及在浅样品阱和读数井之间连通的通道装置，其中样品和反应混合物可以从浅样品很好地进入读取井，而不被任何装置接触。

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