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1. 发明授权

US06514772B2 Semiconductor nanoparticles for analysis of blood cell populations and methods of making same 失效
标题翻译：用于分析血细胞群体的半导体纳米颗粒及其制备方法
公开(公告)号：US06514772B2
公开(公告)日：2003-02-04
申请号：US09813538
申请日：2001-05-08
申请人： Olavi Siiman , Egon Matijevic , Ivan Sondi
发明人： Olavi Siiman , Egon Matijevic , Ivan Sondi
IPC分类号： G01N33543
CPC分类号： B82Y15/00 , G01N33/588 , Y10T428/2982 , Y10T428/2991
摘要： A semiconductor nanoparticle for use in analysis of biological samples is described. This semiconductor nanoparticle is composed of an aminodextran which is bound to at least one nanoparticle of the formula (X Y)n wherein X is selected from the group comprising Cd2+, Hg2+, and Zn2+ and combinations thereof, and Y is selected from the group comprising S2−, Se2− and Te2− and combinations thereof; and n=approximately 50 to 1000. Also provided are methods of making these semiconductor nanoparticles and methods of making conjugates composed of these semiconductor nanoparticles linked to ligands. Also described are uses for the conjugates in a variety of biological assays.
摘要翻译：描述了用于生物样品分析的半导体纳米颗粒。该半导体纳米颗粒由结合至少一个式（XY）n的纳米颗粒的氨基葡聚糖组成，其中X选自Cd2 +，Hg2 +和Zn2 +，以及它们的组合，Y选自S2 - ，Se2-和Te2-及其组合; 并且n =约50至1000.还提供了制备这些半导体纳米颗粒的方法和由与配体连接的这些半导体纳米颗粒组成的共轭物的方法。还描述了在各种生物测定中缀合物的用途。

2. 发明授权

US06235540B1 Semiconductor nanoparticles for analysis of blood cell populations and methods of making same 失效
标题翻译：用于分析血细胞群体的半导体纳米颗粒及其制备方法
公开(公告)号：US06235540B1
公开(公告)日：2001-05-22
申请号：US09281512
申请日：1999-03-30
申请人： Olavi Siiman , Egon Matijevic , Ivan Sondi
发明人： Olavi Siiman , Egon Matijevic , Ivan Sondi
IPC分类号： G01N33551
CPC分类号： B82Y15/00 , G01N33/588 , Y10T428/2982 , Y10T428/2991
摘要： A semiconductor nanoparticle for use in analysis of biological samples is described. This semiconductor nanoparticle is composed of an aminodextran which is bound to at least one nanoparticle of the formula (X Y)n wherein X is selected from the group comprising Cd2+, Hg2+, and Zn2+ and combinations thereof; and Y is selected from the group comprising S2−, Se2− and Te2− and combinations thereof; and n=approximately 50 to 1000. Also provided are methods of making these semiconductor nanoparticles and methods of making conjugates composed of these semiconductor nanoparticles linked to ligands. Also described are uses for the conjugates in a variety of biological assays.
摘要翻译：描述了用于分析生物样品的半导体纳米颗粒。该半导体纳米颗粒由结合至少一种式（X Y）n的纳米颗粒的氨基葡聚糖组成，其中X选自Cd2 +，Hg2 +和Zn2 +，及其组合; Y选自S2-，Se2-和Te2-及其组合; 并且n =约50至1000.还提供了制备这些半导体纳米颗粒的方法和由与配体连接的这些半导体纳米颗粒组成的共轭物的方法。还描述了在各种生物测定中缀合物的用途。

3. 发明申请

US20050026305A1 Aminodextran compositions and conjugates and method of making and using them 失效
标题翻译：氨基葡聚糖组合物和缀合物及其制备和使用方法
公开(公告)号：US20050026305A1
公开(公告)日：2005-02-03
申请号：US10633382
申请日：2003-08-01
申请人： Stephen Ledis , Olavi Siiman , Cynthia Healy
发明人： Stephen Ledis , Olavi Siiman , Cynthia Healy
IPC分类号： A61K31/715 , A61K47/48 , C08B37/00 , C08B37/02 , G01N33/548 , G01N33/53 , G01N33/543 , G01N33/567
CPC分类号： B82Y5/00 , A61K31/715 , A61K47/61 , A61K47/665 , A61K47/6883 , C08B37/0003 , C08B37/0021 , G01N33/548 , Y10S530/812 , Y10S530/813 , Y10S530/815 , Y10S530/816
摘要： A composition containing polydisperse aminodextran polymer molecules is soluble in an aqueous solution at a concentration of 10 mg/ml. The molecules therein have a narrow size distribution characterized by an average molecule mean hydrodynamic diameter of greater than 115 nm, a polydispersity index of between 0.10 and 0.47, an average molecular weight (MW) greater than 3 million daltons, and an amine content of greater than 50 amines per molecule. Similar soluble compositions contain the polymer molecules with an average MW of greater than 7 million daltons. These compositions are useful in forming reagents by conjugation with proteins for labeling cells. Methods of making these compositions and reagents from conventional mixtures of aminodextran polymers involve fractionation on column chromatography.
摘要翻译：含有多分散氨基糊精聚合物分子的组合物可溶于浓度为10mg / ml的水溶液中。其中的分子具有窄尺寸分布，其特征在于平均分子平均流体动力学直径大于115nm，多分散指数为0.10至0.47，平均分子量（MW）大于3百万道尔顿，胺含量较大每分子多于50个胺。类似的可溶性组合物含有平均MW大于700万道尔顿的聚合物分子。这些组合物可用于通过与用于标记细胞的蛋白质缀合形成试剂。从氨基葡聚糖聚合物的常规混合物制备这些组合物和试剂的方法包括在柱色谱上分级分离。

4. 发明授权

US5248772A Formation of colloidal metal dispersions using aminodextrans as reductants and protective agents 失效
标题翻译：使用氨基葡萄糖作为还原剂和保护剂形成胶体金属分散体
公开(公告)号：US5248772A
公开(公告)日：1993-09-28
申请号：US827347
申请日：1992-01-29
申请人： Olavi Siiman , Alexander Burshteyn
发明人： Olavi Siiman , Alexander Burshteyn
IPC分类号： G01N33/532 , A01N59/16 , A61K9/50 , A61K9/62 , A61K9/64 , A61K33/24 , A61K39/395 , A61K47/48 , B01J13/00 , B01J13/04 , C07K1/113 , C07K14/00 , C07K14/42 , C07K16/00 , C08B35/04 , C08B37/02 , G01N33/548 , G01N33/553
CPC分类号： A61K47/48884 , A61K47/48861 , G01N33/548 , G01N33/553 , Y10T428/2989 , Y10T428/2991 , Y10T428/2998
摘要： The invention related generally to the preparation of colloidal metal(O) particles having a crosslinked aminodextran coating with pendent amine groups attached thereto. The aminodextran acts as both a reductant for reducing metal ions to metal(O) particles and as the protective agent which coats the metal(O) particles thus formed. After stabilizing the aminodextran coating by use of a crosslinking agent, the coated particles can be used to covalently bind proteins. The resulting protein containing colloidal particles can be used as markers in optical and electron microscopy, in immunological and biological assays, and possibly as therapeutic agents.
摘要翻译：本发明一般涉及具有连接有悬挂胺基团的交联氨基葡聚糖涂层的胶体金属（O）颗粒的制备。氨基葡聚糖既用作将金属离子还原为金属（O）颗粒的还原剂，也用作涂覆由此形成的金属（O））颗粒的保护剂。通过使用交联剂使氨基葡聚糖涂层稳定后，可以使用包被的颗粒共价结合蛋白质。所得到的含有胶体颗粒的蛋白质可用作光学和电子显微镜，免疫学和生物学测定中的标记物，并可用作治疗剂。

5. 发明授权

US06387622B1 Ligand-aminodextran-marker conjugates and uses thereof 失效
标题翻译：配体 - 氨基葡聚糖标记共轭物及其用途
公开(公告)号：US06387622B1
公开(公告)日：2002-05-14
申请号：US09403919
申请日：1999-10-27
申请人： Olavi Siiman , Alexander Burshteyn , Ravindra Mylvaganam , Robert Raynor , Patricia Roth , Cecilia Smith , Julie Wilkinson
发明人： Olavi Siiman , Alexander Burshteyn , Ravindra Mylvaganam , Robert Raynor , Patricia Roth , Cecilia Smith , Julie Wilkinson
IPC分类号： G01N3368
CPC分类号： G01N33/533 , G01N33/566 , G01N33/56977 , Y10S436/80 , Y10S436/805
摘要： Ligand-aminodextran-(phycobiliprotein or tandem dye) conjugates useful for detection of a desired target biological material by providing an enhanced fluorescent signal are described. Also described is a method for a single-measurement quantification of multiple populations of cells based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked to an aminodextran to a fluorescent phycobiliprotein or tandem dye.
摘要翻译：描述了通过提供增强的荧光信号用于检测所需目标生物材料的配体 - 氨基葡聚糖 - （藻胆蛋白或串联染料）缀合物。还描述了基于不同对细胞群的标记单个测量定量细胞群的方法，每对细胞群含有相互排斥的细胞受体，其以与每个受体的标记配体基本相似的受体密度表达。一个细胞群体用能够结合第一细胞表面受体的配体标记，该配体直接与荧光藻胆蛋白或串联染料缀合; 并且用能够结合第二细胞表面受体的配体标记第二细胞群体，该配体与氨基葡聚糖交联至荧光藻胆蛋白或串联染料。

6. 发明授权

US6074884A Stable protein-nickel particles and methods of production and use thereof 失效
标题翻译：稳定的蛋白质 - 镍颗粒及其生产和使用方法
公开(公告)号：US6074884A
公开(公告)日：2000-06-13
申请号：US947809
申请日：1997-10-09
申请人： Olavi Siiman , Alexander Burshteyn , John A. Maples , James Keller Whitesell
发明人： Olavi Siiman , Alexander Burshteyn , John A. Maples , James Keller Whitesell
IPC分类号： G01N33/532 , G01N33/543 , G01N33/553 , G01N33/569 , G01N33/533
CPC分类号： G01N33/56972 , G01N33/5434 , G01N33/553 , G01N2446/20 , G01N2446/90 , Y10T428/2991 , Y10T428/2998
摘要： A stable protein-coated nickel particle useful in biological assays contains a nickel particle having removed from the surface thereof nickel oxide; a linker attached to said nickel particle, the linker having a free amino group; and a protein attached to said linker by covalently bonding to the free amino group. Methods of producing and using these oxide-free nickel-protein conjugates are disclosed.
摘要翻译：可用于生物测定的稳定的蛋白质包被的镍颗粒含有从其表面除去氧化镍的镍颗粒; 连接到所述镍颗粒的接头，所述接头具有游离氨基; 和通过共价键合到游离氨基上的连接于所述接头的蛋白质。公开了生产和使用这些无氧化物的镍 - 蛋白质缀合物的方法。

7. 发明授权

US5814468A Methods of enumerating receptor molecules for specific binding partners on formed bodies and in solution 失效
标题翻译：列举形成体和溶液中特异性结合配偶体的受体分子的方法
公开(公告)号：US5814468A
公开(公告)日：1998-09-29
申请号：US624014
申请日：1996-03-27
申请人： Olavi Siiman , Alexander Burshteyn , Orlando Concepcion , Meryl Forman
发明人： Olavi Siiman , Alexander Burshteyn , Orlando Concepcion , Meryl Forman
IPC分类号： G01N33/566 , G01N33/533 , C12Q1/70
CPC分类号： G01N33/566
摘要： This invention relates generally to using fluorescent markers (labelled antibodies) and flow cytometry to enumerate the average number of receptors (antigen) on formed bodies (cells) in whole blood, and to evaluate the specific binding constant of the marker for the particular receptors. Mean channel fluorescence intensities of equilibrated marker-cell suspension mixtures, total concentrations of marker, and targeted cell counts obtained by established procedures are used to complete the analyses. The invention further describes a competitive binding assay between marker and unlabelled antibody for receptors shed from the surface of formed bodies into solution and receptors on the formed bodies to analyze for receptors in solution. Moreover, competitive binding assays between marker and unlabelled antibody for receptors on formed bodies in whole blood are described for determination of relative, specific, and average binding constants of unlabelled/labelled antibody for targeted receptors.
摘要翻译：本发明一般涉及使用荧光标记（标记抗体）和流式细胞术来列举全血中成体体（细胞）上的受体（抗原）的平均数目，并评估特定受体的标记物的特异结合常数。平衡的标记 - 细胞悬浮液混合物的平均通道荧光强度，通过建立的程序获得的标记物的总浓度和靶细胞计数用于完成分析。本发明进一步描述了标记物与未标记抗体之间的竞争性结合测定法，用于从形成体表面转移到溶液中的受体和形成体上的受体以分析溶液中的受体。此外，描述了用于确定未标记/标记的靶向受体抗体的相对，特异性和平均结合常数的标记物和未标记抗体之间在全血中形成体上的受体的竞争结合测定。

8. 发明授权

US5658741A Anti-CD3 antibody-aminodextran conjugates for induction of T-cell activation and proliferation 失效
标题翻译：用于诱导T细胞活化和增殖的抗CD3抗体 - 氨基葡聚糖缀合物
公开(公告)号：US5658741A
公开(公告)日：1997-08-19
申请号：US504095
申请日：1995-07-19
申请人： Wade E. Bolton , John A. Maples , Olavi Siiman , Norma Sue Kenyon , Cynthia G. Healy
发明人： Wade E. Bolton , John A. Maples , Olavi Siiman , Norma Sue Kenyon , Cynthia G. Healy
IPC分类号： G01N33/50 , A61K47/48 , A61K49/00 , C07K16/28 , C07K17/10 , C12P21/08 , G01N33/53 , G01N33/531 , G01N33/569 , G01N33/548
CPC分类号： G01N33/56988 , A61K47/48561 , A61K47/48692 , G01N33/531 , G01N33/56972
摘要： The invention describes the use of novel aminodextran compounds containing about 5-20% by weight amine groups to bind a plurality of monoclonal antibodies. The resulting antibody-aminodextran compounds may be used to induce the activation and proliferation of selected mammalian cells. Specific examples are given using an anti-CD3 monoclonal antibody conjugated two aminodextrans containing about 5% and 16%, respectively, by weight amine groups as an agent for inducing the activation and proliferation of T cells.
摘要翻译：本发明描述了使用含有约5-20重量％胺基的新型氨基葡聚糖化合物结合多种单克隆抗体。所得抗体 - 氨基葡聚糖化合物可用于诱导所选哺乳动物细胞的活化和增殖。具体实例是使用分别含有约5％和16％的胺基团的两种氨基葡聚糖作为诱导T细胞活化和增殖的试剂的抗CD3单克隆抗体给予的。

9. 发明授权

US5342754A Preserved, non-infectious control cell for use in the identification of a disease through blood testing 失效
标题翻译：用于通过血液检测鉴定疾病的保存的非感染性对照细胞
公开(公告)号：US5342754A
公开(公告)日：1994-08-30
申请号：US944678
申请日：1992-09-14
申请人： John A. Maples , Robert H. Raynor , Olavi Siiman , Melissa J. Stiglitz , Stephen F. Healy, Jr.
发明人： John A. Maples , Robert H. Raynor , Olavi Siiman , Melissa J. Stiglitz , Stephen F. Healy, Jr.
IPC分类号： G01N33/49 , A01N1/02 , C12N5/08 , C12N5/10 , C12Q1/64 , G01N33/53 , G01N33/569 , G01N33/96 , G01N31/00
CPC分类号： G01N33/96 , G01N2496/05 , Y10T436/101666
摘要： The invention describes the preparation of non-infectious control cells from normal, non-infectious, non-disease altered blood by depletion or augmentation of one or more cell types found in normal blood to reflect a specific disease state. The control cells so produced are preserved and thereafter reconstituted for use in immunological assays.
摘要翻译：本发明描述了通过在正常血液中发现的一种或多种细胞类型的消耗或增加来反映正常的，非传染性的非疾病改变的血液中非感染性对照细胞的制备以反映特定的疾病状态。保存如此产生的对照细胞，然后重构用于免疫测定。

10. 发明授权

US5891741A Antibody-aminodextran-phycobiliprotein conjugates 失效
标题翻译：抗体 - 氨基葡聚糖 - 藻胆蛋白共轭物
公开(公告)号：US5891741A
公开(公告)日：1999-04-06
申请号：US857941
申请日：1997-05-16
申请人： Olavi Siiman , Cecilia Smith , Patricia Roth , Alexander Burshteyn , Robert Raynor
发明人： Olavi Siiman , Cecilia Smith , Patricia Roth , Alexander Burshteyn , Robert Raynor
IPC分类号： G01N33/533 , G01N33/566 , G01N33/569 , G01N33/548
CPC分类号： G01N33/566 , G01N33/533 , G01N33/56977 , Y10S436/80 , Y10S436/805
摘要： The present application discloses dextran crosslinked, antibody-phycobiliprotein conjugates containing up to twenty phycobiliprotein per dextran molecule which conjugates are able to achieve amplification of fluorescence intensity over direct antibody-phycobiliprotein labeled cells of two fold or greater.
摘要翻译：本申请公开了葡聚糖交联的抗体 - 藻胆蛋白共轭物，其每个葡聚糖分子含有多达20个藻胆蛋白，该缀合物能够实现比双倍或更大的直接抗体 - 藻胆蛋白标记的细胞的荧光强度的扩增。

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