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1. 发明授权

US5552086A Immobilized metal colloids on dispersed polymer microspheres 失效
标题翻译：分散的聚合物微球上的固定化金属胶体
公开(公告)号：US5552086A
公开(公告)日：1996-09-03
申请号：US118980
申请日：1993-09-09
申请人： Olavi Siiman , Alexander Burshteyn , Marilyn Cayer
发明人： Olavi Siiman , Alexander Burshteyn , Marilyn Cayer
IPC分类号： G01N33/532 , A01N59/16 , A61K9/50 , A61K9/62 , A61K9/64 , A61K33/24 , A61K39/395 , A61K47/48 , B01J13/00 , B01J13/04 , C07K1/113 , C07K14/00 , C07K14/42 , C07K16/00 , C08B35/04 , C08B37/02 , G01N33/548 , G01N33/553 , B01J13/22 , B32B15/08 , B32B15/16 , G01N33/52
CPC分类号： A61K47/48884 , A61K47/48861 , G01N33/548 , G01N33/553 , Y10T428/2989 , Y10T428/2991 , Y10T428/2998
摘要： The invention describes stable colloidal polymeric particles of 0.2-5.0 microns size which are coated with a first aminodextran layer and a second metallic solid layer, The metal is coated by reduction of a metallic salt or complex by the aminodextran. The metal coated particles, which preferably are gold- or silver-coated polymeric microspheres, produce side scatter and forward shifts in flow cytometry applications that are in agreement with the theoretical shifts predicted for solid gold or silver spheres of similar size in flow cytometry applications,
摘要翻译：本发明描述了0.2-5.0微米尺寸的稳定的胶态聚合物颗粒，其被第一氨基葡聚糖层和第二金属固体层涂覆。金属通过氨基葡聚糖还原金属盐或络合物而被包被。优选金或银涂覆的聚合物微球体的金属涂覆颗粒在流式细胞术应用中产生侧向散射和向前移位，这与流式细胞术应用中类似尺寸的固体金或银球预测的理论位移一致，

2. 发明授权

US5248772A Formation of colloidal metal dispersions using aminodextrans as reductants and protective agents 失效
标题翻译：使用氨基葡萄糖作为还原剂和保护剂形成胶体金属分散体
公开(公告)号：US5248772A
公开(公告)日：1993-09-28
申请号：US827347
申请日：1992-01-29
申请人： Olavi Siiman , Alexander Burshteyn
发明人： Olavi Siiman , Alexander Burshteyn
IPC分类号： G01N33/532 , A01N59/16 , A61K9/50 , A61K9/62 , A61K9/64 , A61K33/24 , A61K39/395 , A61K47/48 , B01J13/00 , B01J13/04 , C07K1/113 , C07K14/00 , C07K14/42 , C07K16/00 , C08B35/04 , C08B37/02 , G01N33/548 , G01N33/553
CPC分类号： A61K47/48884 , A61K47/48861 , G01N33/548 , G01N33/553 , Y10T428/2989 , Y10T428/2991 , Y10T428/2998
摘要： The invention related generally to the preparation of colloidal metal(O) particles having a crosslinked aminodextran coating with pendent amine groups attached thereto. The aminodextran acts as both a reductant for reducing metal ions to metal(O) particles and as the protective agent which coats the metal(O) particles thus formed. After stabilizing the aminodextran coating by use of a crosslinking agent, the coated particles can be used to covalently bind proteins. The resulting protein containing colloidal particles can be used as markers in optical and electron microscopy, in immunological and biological assays, and possibly as therapeutic agents.
摘要翻译：本发明一般涉及具有连接有悬挂胺基团的交联氨基葡聚糖涂层的胶体金属（O）颗粒的制备。氨基葡聚糖既用作将金属离子还原为金属（O）颗粒的还原剂，也用作涂覆由此形成的金属（O））颗粒的保护剂。通过使用交联剂使氨基葡聚糖涂层稳定后，可以使用包被的颗粒共价结合蛋白质。所得到的含有胶体颗粒的蛋白质可用作光学和电子显微镜，免疫学和生物学测定中的标记物，并可用作治疗剂。

3. 发明授权

US5891741A Antibody-aminodextran-phycobiliprotein conjugates 失效
标题翻译：抗体 - 氨基葡聚糖 - 藻胆蛋白共轭物
公开(公告)号：US5891741A
公开(公告)日：1999-04-06
申请号：US857941
申请日：1997-05-16
申请人： Olavi Siiman , Cecilia Smith , Patricia Roth , Alexander Burshteyn , Robert Raynor
发明人： Olavi Siiman , Cecilia Smith , Patricia Roth , Alexander Burshteyn , Robert Raynor
IPC分类号： G01N33/533 , G01N33/566 , G01N33/569 , G01N33/548
CPC分类号： G01N33/566 , G01N33/533 , G01N33/56977 , Y10S436/80 , Y10S436/805
摘要： The present application discloses dextran crosslinked, antibody-phycobiliprotein conjugates containing up to twenty phycobiliprotein per dextran molecule which conjugates are able to achieve amplification of fluorescence intensity over direct antibody-phycobiliprotein labeled cells of two fold or greater.
摘要翻译：本申请公开了葡聚糖交联的抗体 - 藻胆蛋白共轭物，其每个葡聚糖分子含有多达20个藻胆蛋白，该缀合物能够实现比双倍或更大的直接抗体 - 藻胆蛋白标记的细胞的荧光强度的扩增。

4. 发明授权

US5062991A In situ use of gelatin in the preparation of uniform ferrite particles 失效
标题翻译：原位使用明胶制备均匀的铁氧体颗粒
公开(公告)号：US5062991A
公开(公告)日：1991-11-05
申请号：US532434
申请日：1990-06-04
申请人： Olavi Siiman , Alexander Burshteyn
发明人： Olavi Siiman , Alexander Burshteyn
IPC分类号： B01J13/06 , B01J13/00 , B03C1/01 , H01F1/06 , H01F1/26 , H01F1/44
CPC分类号： B01J13/0013 , B03C1/01 , H01F1/061 , H01F1/445 , Y10T428/2991
摘要： A method is described for the preparation of uniform colloidal particles of ferrites, containing manganese (II), zinc(II), barium(II), iron(II), cobalt(II), or nickel(II), or a mixture of manganese(II) and zinc(II), at a relatively low temperature in the presence of a gelatin solution which acts as a support vehicle for the nucleation and growth of colloidal particles of metal oxide and for dispersion as separate single particles.
摘要翻译：描述了制备含有锰（II），锌（II），钡（II），铁（II），钴（II）或镍（II））的铁素体的均匀胶态粒子的方法，或者锰（II）和锌（II）在相对较低的温度下，在明胶溶液的存在下，其作为用于金属氧化物的胶体颗粒的成核和生长并用作分散的单个颗粒的载体载体。

5. 发明授权

US06387622B1 Ligand-aminodextran-marker conjugates and uses thereof 失效
标题翻译：配体 - 氨基葡聚糖标记共轭物及其用途
公开(公告)号：US06387622B1
公开(公告)日：2002-05-14
申请号：US09403919
申请日：1999-10-27
申请人： Olavi Siiman , Alexander Burshteyn , Ravindra Mylvaganam , Robert Raynor , Patricia Roth , Cecilia Smith , Julie Wilkinson
发明人： Olavi Siiman , Alexander Burshteyn , Ravindra Mylvaganam , Robert Raynor , Patricia Roth , Cecilia Smith , Julie Wilkinson
IPC分类号： G01N3368
CPC分类号： G01N33/533 , G01N33/566 , G01N33/56977 , Y10S436/80 , Y10S436/805
摘要： Ligand-aminodextran-(phycobiliprotein or tandem dye) conjugates useful for detection of a desired target biological material by providing an enhanced fluorescent signal are described. Also described is a method for a single-measurement quantification of multiple populations of cells based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked to an aminodextran to a fluorescent phycobiliprotein or tandem dye.
摘要翻译：描述了通过提供增强的荧光信号用于检测所需目标生物材料的配体 - 氨基葡聚糖 - （藻胆蛋白或串联染料）缀合物。还描述了基于不同对细胞群的标记单个测量定量细胞群的方法，每对细胞群含有相互排斥的细胞受体，其以与每个受体的标记配体基本相似的受体密度表达。一个细胞群体用能够结合第一细胞表面受体的配体标记，该配体直接与荧光藻胆蛋白或串联染料缀合; 并且用能够结合第二细胞表面受体的配体标记第二细胞群体，该配体与氨基葡聚糖交联至荧光藻胆蛋白或串联染料。

6. 发明授权

US6074884A Stable protein-nickel particles and methods of production and use thereof 失效
标题翻译：稳定的蛋白质 - 镍颗粒及其生产和使用方法
公开(公告)号：US6074884A
公开(公告)日：2000-06-13
申请号：US947809
申请日：1997-10-09
申请人： Olavi Siiman , Alexander Burshteyn , John A. Maples , James Keller Whitesell
发明人： Olavi Siiman , Alexander Burshteyn , John A. Maples , James Keller Whitesell
IPC分类号： G01N33/532 , G01N33/543 , G01N33/553 , G01N33/569 , G01N33/533
CPC分类号： G01N33/56972 , G01N33/5434 , G01N33/553 , G01N2446/20 , G01N2446/90 , Y10T428/2991 , Y10T428/2998
摘要： A stable protein-coated nickel particle useful in biological assays contains a nickel particle having removed from the surface thereof nickel oxide; a linker attached to said nickel particle, the linker having a free amino group; and a protein attached to said linker by covalently bonding to the free amino group. Methods of producing and using these oxide-free nickel-protein conjugates are disclosed.
摘要翻译：可用于生物测定的稳定的蛋白质包被的镍颗粒含有从其表面除去氧化镍的镍颗粒; 连接到所述镍颗粒的接头，所述接头具有游离氨基; 和通过共价键合到游离氨基上的连接于所述接头的蛋白质。公开了生产和使用这些无氧化物的镍 - 蛋白质缀合物的方法。

7. 发明授权

US5814468A Methods of enumerating receptor molecules for specific binding partners on formed bodies and in solution 失效
标题翻译：列举形成体和溶液中特异性结合配偶体的受体分子的方法
公开(公告)号：US5814468A
公开(公告)日：1998-09-29
申请号：US624014
申请日：1996-03-27
申请人： Olavi Siiman , Alexander Burshteyn , Orlando Concepcion , Meryl Forman
发明人： Olavi Siiman , Alexander Burshteyn , Orlando Concepcion , Meryl Forman
IPC分类号： G01N33/566 , G01N33/533 , C12Q1/70
CPC分类号： G01N33/566
摘要： This invention relates generally to using fluorescent markers (labelled antibodies) and flow cytometry to enumerate the average number of receptors (antigen) on formed bodies (cells) in whole blood, and to evaluate the specific binding constant of the marker for the particular receptors. Mean channel fluorescence intensities of equilibrated marker-cell suspension mixtures, total concentrations of marker, and targeted cell counts obtained by established procedures are used to complete the analyses. The invention further describes a competitive binding assay between marker and unlabelled antibody for receptors shed from the surface of formed bodies into solution and receptors on the formed bodies to analyze for receptors in solution. Moreover, competitive binding assays between marker and unlabelled antibody for receptors on formed bodies in whole blood are described for determination of relative, specific, and average binding constants of unlabelled/labelled antibody for targeted receptors.
摘要翻译：本发明一般涉及使用荧光标记（标记抗体）和流式细胞术来列举全血中成体体（细胞）上的受体（抗原）的平均数目，并评估特定受体的标记物的特异结合常数。平衡的标记 - 细胞悬浮液混合物的平均通道荧光强度，通过建立的程序获得的标记物的总浓度和靶细胞计数用于完成分析。本发明进一步描述了标记物与未标记抗体之间的竞争性结合测定法，用于从形成体表面转移到溶液中的受体和形成体上的受体以分析溶液中的受体。此外，描述了用于确定未标记/标记的靶向受体抗体的相对，特异性和平均结合常数的标记物和未标记抗体之间在全血中形成体上的受体的竞争结合测定。

8. 发明授权

US5994089A Simultaneous analyses of white blood cell subsets using multi-color, multi-intensity fluorescent markers in flow cytometry 失效
标题翻译：在流式细胞术中使用多色多重荧光标记物同时分析白细胞亚群
公开(公告)号：US5994089A
公开(公告)日：1999-11-30
申请号：US976031
申请日：1997-11-21
申请人： Olavi Siiman , Alexander Burshteyn , Julie Wilkinson , Ravindra Mylvaganam
发明人： Olavi Siiman , Alexander Burshteyn , Julie Wilkinson , Ravindra Mylvaganam
IPC分类号： G01N33/548 , G01N15/14 , G01N33/483 , G01N33/533 , G01N33/543 , G01N33/566 , G01N33/569
CPC分类号： G01N33/533 , G01N33/566 , G01N33/56977 , Y10S436/80 , Y10S436/805
摘要： A method for a single-measurement quantification of multiple populations of white blood cells (WBC) is based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked by an aminodextran to a fluorescent phycobiliprotein or tandem dye. The phycobiliproteins upon laser excitation produce a different detectable fluorescence intensity for each cell population. Use of such pairs of conjugates enable two populations of cells with similar receptor densities to be distinguished with the use of a single color marker. Further use of additional ligands to other cell surface receptors and additional phycobiliprotein or tandem dye, or other markers in the same manner, enables the simultaneous quantification of up to 45 different cell populations with one laser line and four fluorescent colors.
摘要翻译：用于单次测量多种白细胞群体（WBC）的方法基于不同细胞群体对的标记，每对含有相互排斥的细胞受体，其以基本相似的受体密度表达，其中每个具有标记的配体受体。一个细胞群体用能够结合第一细胞表面受体的配体标记，该配体直接与荧光藻胆蛋白或串联染料缀合; 并且用能够结合第二细胞表面受体的配体标记第二细胞群，所述配体通过氨基葡聚糖与荧光藻胆蛋白或串联染料交联。激光激发后的藻胆蛋白对每个细胞群产生不同的可检测荧光强度。使用这样的缀合物对使得具有相似受体密度的两个细胞群可以使用单一颜色标记来区分。以其他细胞表面受体和附加的藻胆蛋白或串联染料或其他标记物以其他方式进一步使用额外的配体使得能够用一条激光线和四种荧光颜色同时定量多达45个不同的细胞群体。

9. 发明授权

US5707877A Biodegradable gelatin-aminodextran particle coatings of and processes for making same 失效
公开(公告)号：US5707877A
公开(公告)日：1998-01-13
申请号：US493973
申请日：1995-06-23
申请人： Olavi Siiman , Alexander Burshteyn , Ravinder K. Gupta
发明人： Olavi Siiman , Alexander Burshteyn , Ravinder K. Gupta
IPC分类号： G01N33/543 , B01J13/00 , B01J13/02 , B01J13/14 , B03C1/01 , G01N33/531 , G01N33/544 , G01N33/553
CPC分类号： B03C1/01 , G01N33/531 , G01N33/54313 , G01N33/5434 , G01N33/54393 , G01N2446/20 , G01N2446/30 , G01N2446/84 , G01N2446/86 , G01N2446/90 , Y10S530/81 , Y10S530/812 , Y10S530/813 , Y10T428/2991
摘要： The invention relates generally to colloidal particle having a core material and a gelatin/aminodextran coating with pendent functional groups attached thereto. Biological substances or molecules, especially monoclonal antibodies, may be attached to said particles. The monoclonal antibody containing particles are useful in a variety of positive and negative biological assays.

10. 发明授权

US5639620A Polymeric particles having a biodegradable gelatin or aminodextran coating and process for making same 失效
标题翻译：具有可生物降解的明胶或氨基葡聚糖涂层的聚合物颗粒及其制备方法
公开(公告)号：US5639620A
公开(公告)日：1997-06-17
申请号：US336725
申请日：1994-11-09
申请人： Olavi Siiman , Alexander Burshteyn , Ravinder K. Gupta
发明人： Olavi Siiman , Alexander Burshteyn , Ravinder K. Gupta
IPC分类号： G01N33/531 , G01N33/543 , G01N33/545 , G01N33/546 , G01N33/547 , G01N33/548
CPC分类号： G01N33/5434 , G01N33/531 , G01N33/54313 , G01N33/54393 , G01N2446/20 , G01N2446/30 , G01N2446/84 , G01N2446/86 , G01N2446/90 , Y10S977/829 , Y10S977/897 , Y10S977/898 , Y10T428/2991 , Y10T428/2998 , Y10T436/24
摘要： Gelatin and aminodextran coated polymer core particles useful in immunoassays and methods of making the same are disclosed. The preparation of aminodextrans having varying amounts of amine groups is also described, as is a method of crosslinking gelatin and aminodextran without the use of a stabilizer.
摘要翻译：公开了可用于免疫测定的明胶和氨基葡聚糖包被的聚合物核心颗粒及其制备方法。还描述了具有不同量的胺基团的氨基葡聚糖的制备，以及不使用稳定剂交联明胶和氨基葡聚糖的方法。

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