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1. 发明公开

EP2825652A4 COMPOSITION AND METHOD FOR DIVERSIFICATION OF TARGET SEQUENCES 有权
标题翻译： ZUSAMMENSETZUNG UND VERFAHREN ZUR DIVERSIFIZIERUNG VON ZIELSEQUENZEN
公开(公告)号：EP2825652A4
公开(公告)日：2015-10-07
申请号：EP13761578
申请日：2013-03-13
申请人： OMEROS CORP , UNIV WASHINGTON CT COMMERCIALI
发明人： CUMMINGS W JASON , TJOELKER LARRY W , WOOD CHRISTI L , YABUKI MUNEHISA , ALLISON DANIEL S , LEPPARD JOHN B , MAIZELS NANCY
IPC分类号： C12N15/63 , C12N15/13 , C12N15/70 , C12N15/79
CPC分类号： C12N15/1082 , C07K16/00 , C07K16/2863 , C07K16/30 , C07K2317/24 , C07K2317/92
摘要： The disclosure relates generally to the targeting of genes to, and their integration into, an immunoglobulin (antibody) heavy chain locus. In particular, the methods described herein contemplate replacing the single rearranged heavy chain V, D, and J genes of a B cell lymphoma such as DT40 with independently rearranged VH-D-JH genes of chicken, in a system for generating immunoglobulin diversity. Also contemplated is replacement of the chicken VH-D-JH with rearranged VH-D-JH genes of other vertebrates including human in a system for generating immunoglobulin diversity, with the exception of any substitution disclosed and claimed in PCT application WO 2009/029315 A2. Also described is construction of a diverse chicken immunoglobulin heavy chain VDJ library in DT40 by homologous gene replacement of the single endogenous rearranged VDJ gene with a chicken VDJ repertoire using the described targeting vectors.
摘要翻译：本公开一般涉及基因的靶向，并且与免疫球蛋白（抗体）重链基因座的融合。特别地，本文描述的方法考虑在用于产生免疫球蛋白多样性的系统中将具有独立重排的鸡的VH-D-JH基因的B细胞淋巴瘤（例如DT40）的单重排列重链V，D和J基因替换为独立重排的VH-D-JH基因。还考虑到除了用于产生免疫球蛋白多样性的系统中，包括人的其它脊椎动物的重排的VH-D-JH基因的鸡VH-D-JH的替换，除了PCT申请WO 2009/029315A2中公开和要求的任何替代物。还描述了通过使用所述靶向载体与单个内源重排的VDJ基因与鸡VDJ库的同源基因替换来构建DT40中的多种鸡免疫球蛋白重链VDJ文库。

2. 发明专利

AU2013232114A2 Composition and method for diversification of target sequences 未知
公开(公告)号：AU2013232114A2
公开(公告)日：2014-10-23
申请号：AU2013232114
申请日：2013-03-13
申请人： OMEROS CORP , UNIV WASHINGTON CT COMMERCIALI
发明人： CUMMINGS W JASON , TJOELKER LARRY W , WOOD CHRISTI L , YABUKI MUNEHISA , ALLISON DANIEL S , LEPPARD JOHN B , MAIZELS NANCY
IPC分类号： C12N15/63 , C12N15/13 , C12N15/70 , C12N15/79
摘要： The disclosure relates generally to the targeting of genes to, and their integration into, an innnnunoglobulin (antibody) heavy chain locus. In particular, the methods described herein contemplate replacing the single rearranged heavy chain V, D, and J genes of a B cell lymphoma such as DT40 with independently rearranged V

3. 发明专利

AU2013232114A1 Composition and method for diversification of target sequences 未知
公开(公告)号：AU2013232114A1
公开(公告)日：2014-10-16
申请号：AU2013232114
申请日：2013-03-13
申请人： OMEROS CORP , UNIV WASHINGTON CT COMMERCIALI
发明人： CUMMINGS W JASON , TJOELKER LARRY W , WOOD CHRISTI L , YABUKI MUNEHISA , ALLISON DANIEL S , LEPPARD JOHN B , MAIZELS NANCY
IPC分类号： C12N15/63 , C12N15/13 , C12N15/70 , C12N15/79
摘要： The disclosure relates generally to the targeting of genes to, and their integration into, an innnnunoglobulin (antibody) heavy chain locus. In particular, the methods described herein contemplate replacing the single rearranged heavy chain V, D, and J genes of a B cell lymphoma such as DT40 with independently rearranged V

4. 发明专利

CA2865506A1 COMPOSITION AND METHOD FOR DIVERSIFICATION OF TARGET SEQUENCES 未知
公开(公告)号：CA2865506A1
公开(公告)日：2013-09-19
申请号：CA2865506
申请日：2013-03-13
申请人： OMEROS CORP , UNIV WASHINGTON CT COMMERCIALI
发明人： CUMMINGS W JASON , TJOELKER LARRY W , WOOD CHRISTI L , YABUKI MUNEHISA , ALLISON DANIEL S , LEPPARD JOHN B , MAIZELS NANCY
IPC分类号： C12N15/63 , C12N15/13 , C12N15/70 , C12N15/79
摘要： The disclosure relates generally to the targeting of genes to, and their integration into, an innnnunoglobulin (antibody) heavy chain locus. In particular, the methods described herein contemplate replacing the single rearranged heavy chain V, D, and J genes of a B cell lymphoma such as DT40 with independently rearranged VH-D-JH genes of chicken, in a system for generating immunoglobulin diversity. Also contemplated is replacement of the chicken VH-D-JH with rearranged VH-D-JH genes of other vertebrates including human in a system for generating immunoglobulin diversity, with the exception of any substitution disclosed and claimed in PCT application WO 2009/029315 A2. Regions upstream of the rearranged VH gene, and downstream of the rearranged JH genes, are identified and incorporated into the immunoglobulin heavy chain gene-targeting vectors for efficient gene replacement. Also described is construction of a diverse chicken immunoglobulin heavy chain VDJ library in DT40 by homologous gene replacement of the single endogenous rearranged VDJ gene with a chicken VDJ repertoire using the described targeting vectors. The methods are useful for generating a diverse library of immunoglobulins that can be screened for binding to desired target antigens. Compositions include the unique targeting vectors, the diverse chicken heavy chain VDJ library in the targeting vectors, and the diverse chicken VDJ DT40 library that is created by homologous gene replacement of the single endogenous rearranged VDJ gene with a chicken VDJ repertoire, using the targeting vectors.

5. 发明专利

AU2013232114B2 Composition and method for diversification of target sequences 未知
公开(公告)号：AU2013232114B2
公开(公告)日：2018-10-18
申请号：AU2013232114
申请日：2013-03-13
申请人： OMEROS CORP , UNIV WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
发明人： CUMMINGS W JASON , TJOELKER LARRY W , WOOD CHRISTI L , YABUKI MUNEHISA , ALLISON DANIEL S , LEPPARD JOHN B , MAIZELS NANCY
IPC分类号： C12N15/63 , C12N15/13 , C12N15/70 , C12N15/79
摘要： The disclosure relates generally to the targeting of genes to, and their integration into, an innnnunoglobulin (antibody) heavy chain locus. In particular, the methods described herein contemplate replacing the single rearranged heavy chain V, D, and J genes of a B cell lymphoma such as DT40 with independently rearranged V

6. 发明专利

CA2865506C COMPOSITION AND METHOD FOR DIVERSIFICATION OF TARGET SEQUENCES 未知
公开(公告)号：CA2865506C
公开(公告)日：2021-07-06
申请号：CA2865506
申请日：2013-03-13
申请人： OMEROS CORP , UNIV WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
发明人： CUMMINGS W JASON , TJOELKER LARRY W , WOOD CHRISTI L , YABUKI MUNEHISA , ALLISON DANIEL S , LEPPARD JOHN B , MAIZELS NANCY
IPC分类号： C12N15/63 , C12N15/13 , C12N15/70 , C12N15/79
摘要： The disclosure relates generally to the targeting of genes to, and their integration into, an innnnunoglobulin (antibody) heavy chain locus. In particular, the methods described herein contemplate replacing the single rearranged heavy chain V, D, and J genes of a B cell lymphoma such as DT40 with independently rearranged VH-D-JH genes of chicken, in a system for generating immunoglobulin diversity. Also contemplated is replacement of the chicken VH-D-JH with rearranged VH-D-JH genes of other vertebrates including human in a system for generating immunoglobulin diversity, with the exception of any substitution disclosed and claimed in PCT application WO 2009/029315 A2. Regions upstream of the rearranged VH gene, and downstream of the rearranged JH genes, are identified and incorporated into the immunoglobulin heavy chain gene-targeting vectors for efficient gene replacement. Also described is construction of a diverse chicken immunoglobulin heavy chain VDJ library in DT40 by homologous gene replacement of the single endogenous rearranged VDJ gene with a chicken VDJ repertoire using the described targeting vectors. The methods are useful for generating a diverse library of immunoglobulins that can be screened for binding to desired target antigens. Compositions include the unique targeting vectors, the diverse chicken heavy chain VDJ library in the targeting vectors, and the diverse chicken VDJ DT40 library that is created by homologous gene replacement of the single endogenous rearranged VDJ gene with a chicken VDJ repertoire, using the targeting vectors.

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