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    • 3. 发明申请
    • DNA ENCODING OAT ACETYL CoA CARBOXYLASE
    • DNA编码OAT乙酰辅酶A羧酸酶
    • WO9967367A9
    • 2000-07-06
    • PCT/US9914022
    • 1999-06-22
    • UNIV MINNESOTAUS AGRICULTUREEGLI MARGARET AGROH SUSANNEKIANIAN SHAHRYAR FPHILLIPS RONALD LSOMERS DAVID AFULCHER R GARY
    • EGLI MARGARET AGROH SUSANNEKIANIAN SHAHRYAR FPHILLIPS RONALD LSOMERS DAVID AFULCHER R GARY
    • C12N9/00C12N15/82A01H5/00A01H5/10
    • C12N9/93C12N15/8247C12N15/8274
    • The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of imparting herbicide tolerance to a plant includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme. Also provided is a partial DNA sequence encoding oat acetyl CoA carboxylase.
    • 本发明提供玉米乙酰CoA羧化酶的完整cDNA序列,以及通过在植物细胞中引入和表达植物乙酰CoA羧化酶基因来赋予除草剂耐受性和/或改变植物油含量的方法。 对植物赋予除草剂耐受性的方法包括以下步骤:将编码植物乙酰辅酶A羧化酶的表达盒或与植物乙酰辅酶A羧化酶基因互补的反义DNA序列引入到与植物细胞中有功能的启动子连接的植物乙酰辅酶A羧化酶基因, 植物组织的细胞并以有效使得乙酰辅酶A羧化酶和/或植物细胞对除草剂具有耐受性的量表达植物乙酰辅酶A羧化酶基因。 改变植物油含量的方法包括将表达盒引入植物细胞并以有效改变细胞油含量的量表达乙酰CoA羧化酶基因的步骤。 也可以将表达盒引入其他宿主细胞中以增加植物乙酰CoA羧化酶的产量,使得结晶的酶可用于筛选和鉴定结合并抑制酶的其它除草剂。 还提供了编码燕麦乙酰辅酶A羧化酶的部分DNA序列。