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    • 2. 发明申请
    • METHOD FOR MEASURING SUBSTANCE TO BE MEASURED USING FLUORESCENT PARTICLES, TEST SUBSANCE MEASUREMENT CHIP, AND TEST SUBSANCE MEASUREMENT KIT
    • 使用荧光颗粒测量物质的方法,测试接口测量芯片和测试位置测量套件
    • US20130078738A1
    • 2013-03-28
    • US13614806
    • 2012-09-13
    • Yuya WATANABENoriyuki KASAGI
    • Yuya WATANABENoriyuki KASAGI
    • G01N21/64
    • G01N33/54366G01N33/54313G01N33/585
    • A method for measuring a test substance including (1) reacting fluorescent particles and a test substance by bringing the test substance and the fluorescent particles into contact with a test area and a control area present on a substrate, (2) removing the unreacted fluorescent particles, (3) measuring the fluorescence of the fluorescent particles of the test area and the control area, and (4) correcting a fluorescence signal value in the test area using a fluorescence signal value in the control area, wherein the fluorescent particles are binding substance-labeling fluorescent particles in which one or more of a first binding substance which can bind to the test substance, and a third binding substance which can bind to a second binding substance and which does not bind to the test substance are bound, and the second binding substance which can bind to the first binding substance is immobilized on the control area.
    • 一种测试物质的测量方法,包括:(1)通过使测试物质和荧光颗粒与存在于基底上的测试区域和控制区域接触使荧光颗粒和测试物质反应,(2)除去未反应的荧光颗粒 ,(3)测量测试区域和控制区域的荧光颗粒的荧光,以及(4)使用控制区域中的荧光信号值校正测试区域中的荧光信号值,其中荧光颗粒是结合物质 标记荧光颗粒,其中可以结合测试物质的一种或多种第一结合物质和可结合第二结合物质并且不结合测试物质的第三结合物质结合,第二种 可以结合第一结合物质的结合物质固定在对照区域上。