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    • 2. 发明授权
    • Optical evaluation using a hologram barrier filter
    • 使用全息屏障滤光片进行光学评估
    • US5166813A
    • 1992-11-24
    • US551634
    • 1990-07-11
    • Michael H. Metz
    • Michael H. Metz
    • G01N21/64G02B5/32
    • G01N21/645G01N21/6428G02B5/32G01N2021/6463G01N2021/6471G01N2021/6476Y10S435/808
    • Optical detection apparatus and process which, in its optimum configuration, utilizes holographic optical elements both as a dichroic beamsplitter and as a selective notch-type barrier filter to enhance the amount of light to reach the detector while barring undesirable wavelengths from reaching the detector. The apparatus, when incorporated into a fluorescent-light detecting process, has the advantage of minimizing loss of light suffered because of a small Stokes shift, which shift occurs between the wavelength of the light transmitted to a sample target area and the increased wavelength of fluorescent light emitted from the sample for collection by a detection device. This advantage which is allowed by sharper cut-on of optical transmission properties relative to wavelength by a barrier filter (or filters) comprised of holographic optical element (or elements) provides an important increase in the fluorescence-collecting capacity of the apparatus and the sensitivity of detection processes carried out with the apparatus. Those optical detection systems which use a dichroic beamsplitter benefit by using a holographic optical element which can serve multiple purposes by acting as an exciter filter, selecting the wavelength band from the input light, as a highly efficient mirror to enhance the amount of light reaching the sample and, also, as a barrier filter as described above.
    • 在其最佳配置中,光学检测装置和工艺利用全息光学元件作为二向色分束器和作为选择性陷波型屏障滤光器,以增强到达检测器的光量,同时阻止不期望的波长到达检测器。 该装置并入荧光检测过程中具有最小化斯托克斯偏移所造成的光损失的优点,该偏移发生在透射到样本目标区域的光的波长与荧光增强波长之间 从样品发射的光由检测装置收集。 通过由全息光学元件(或元件)组成的屏障滤光片(或滤光片),相对于波长的光学透射特性的更明显的切割允许的这个优点提供了装置的荧光收集能力和灵敏度的重要增加 的检测过程。 使用二向色分束器的那些光学检测系统通过使用通过充当激励器滤波器而可以用于多种目的的全息光学元件,从输入光中选择波长带作为高效反射镜,以增强到达光的量 样品以及如上所述的屏障过滤器。