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1. 发明申请

US20080233642A9 MAMMALIAN IMMORTALIZED LIVER CELL 审中-公开
标题翻译： MAMMALIAN IMMURALIZED LIVER CELL
公开(公告)号：US20080233642A9
公开(公告)日：2008-09-25
申请号：US10169084
申请日：2002-03-15
申请人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara , Toshinori Totsugawa
发明人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara , Toshinori Totsugawa
IPC分类号： C12N5/08
CPC分类号： C12N5/067 , A61K35/12 , C12N2503/00 , C12N2503/02 , C12N2510/04
摘要： The present invention provides a mammalian immortalized liver cell obtained by transferring a cell proliferation factor gene located between a pair of site-specific recombination sequences into a mammalian liver cell.
摘要翻译：本发明提供通过将位于一对位点特异性重组序列之间的细胞增殖因子基因转移到哺乳动物肝细胞中而获得的哺乳动物永生化肝细胞。

2. 发明授权

US07521234B2 Mammalian immortalized liver cell 失效
标题翻译：哺乳动物永生化肝细胞
公开(公告)号：US07521234B2
公开(公告)日：2009-04-21
申请号：US10169084
申请日：2002-03-15
申请人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara , Toshinori Totsugawa
发明人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara , Toshinori Totsugawa
IPC分类号： C12N5/08
CPC分类号： C12N5/067 , A61K35/12 , C12N2503/00 , C12N2503/02 , C12N2510/04
摘要： The present invention provides a mammalian immortalized liver cell obtained by transferring a cell proliferation factor gene located between a pair of site-specific recombination sequences into a mammalian liver cell.
摘要翻译：本发明提供通过将位于一对位点特异性重组序列之间的细胞增殖因子基因转移到哺乳动物肝细胞中而获得的哺乳动物永生化肝细胞。

3. 发明授权

US07033744B2 Method for proliferating a liver cell, a liver cell obtained thereby, and use thereof 失效
标题翻译：用于增殖肝细胞的方法，由此获得的肝细胞及其用途
公开(公告)号：US07033744B2
公开(公告)日：2006-04-25
申请号：US10169085
申请日：2002-03-15
申请人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara , Toshinori Totsugawa
发明人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara , Toshinori Totsugawa
IPC分类号： A01N1/00
CPC分类号： C12N5/067 , A61K35/12 , C12N2510/02 , C12N2510/04
摘要： The present invention provides a method comprising a step of transferring a cell proliferation factor gene into a mammalian liver cell to obtain an immortalized liver cell, a step of proliferating the immortalized liver cell, and a step of removing the cell proliferation factor gene from the immortalized liver cell; a large number of liver cells obtained thereby; and a treating agent and a artificial liver comprising obtained liver cell.
摘要翻译：本发明提供一种方法，其包括将细胞增殖因子基因转移到哺乳动物肝细胞中以获得永生化肝细胞，增殖永生化肝细胞的步骤和从永生化细胞中除去细胞增殖因子基因的步骤肝细胞; 由此获得大量肝细胞; 以及包含获得的肝细胞的处理剂和人造肝。

4. 发明授权

US06645763B2 Immortalized bone marrow mesenchymal stem cell 失效
标题翻译：永生化骨髓间充质干细胞
公开(公告)号：US06645763B2
公开(公告)日：2003-11-11
申请号：US09975304
申请日：2001-10-12
申请人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara
发明人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara
IPC分类号： C12N508
CPC分类号： C12N9/1276 , C12N5/0663 , C12N2510/04 , C12N2799/027
摘要： The present invention provides a safe immortalized bone marrow mesenchymal stem cell obtained by transferring a cell proliferation factor gene inserted between a pair of site-specific recombination sequences to a bone marrow mesenchymal stem cell.
摘要翻译：本发明提供了通过将插入在一对位点特异性重组序列之间的细胞增殖因子基因转移到骨髓间充质干细胞而获得的安全永生化骨髓间充质干细胞。

5. 发明授权

US08034620B2 Lentiviral packaging cells and uses therefor 有权
标题翻译：慢病毒包装细胞及其用途
公开(公告)号：US08034620B2
公开(公告)日：2011-10-11
申请号：US11983497
申请日：2007-11-09
申请人： Philippe Leboulch , Karen Westerman
发明人： Philippe Leboulch , Karen Westerman
IPC分类号： C12N15/87
CPC分类号： C12N15/86 , A61K48/00 , C12N2740/16043 , C12N2740/16052
摘要： Novel packaging cell lines which produce recombinant retrovirus, free of detectable helper-virus are disclosed. Also disclosed are methods of making the cell lines and methods of producing recombinant retroviruses from the cell lines. Retroviruses produced by the cell lines include lentiviruses, such as HIV, capable of transfering heterologous DNA to a wide range of non-dividing cells. The packaging cells contain at least three vectors which collectively encode retroviral gag, pol, and env proteins, wherein the gag and pol genes are separated, in part, onto two or more different vectors. This is made possible by fusing Vpr or Vpx to pol proteins separated from gag so that the proteins are targeted to assembling virions. Among other advantages, the packaging cells provide the benefit of increased safety when used in human gene therapy by virtually eliminating the possibility of molecular recombination leading to production of replication competent helper virus.
摘要翻译：公开了产生不含可检测的辅助病毒的重组逆转录病毒的新型包装细胞系。还公开了制备细胞系的方法和从细胞系产生重组逆转录病毒的方法。由细胞系产生的逆转录病毒包括能够将异源DNA转移到广泛的非分裂细胞的慢病毒，例如HIV。包装细胞含有至少三个共同编码逆转录病毒gag，pol和env蛋白的载体，其中gag和pol基因部分地分离到两个或更多个不同的载体上。这可以通过将Vpr或Vpx融合到从gag分离的pol蛋白，使得蛋白质靶向于组装病毒粒子。除了其它优点之外，当用于人类基因治疗时，包装细胞通过实际上消除分子重组导致产生复制能力的辅助病毒的可能性而提供了增加的安全性的益处。

6. 发明申请

US20070190030A1 Method of treating arthritis using lentiviral vectors in gene therapy 审中-公开
标题翻译：在基因治疗中使用慢病毒载体治疗关节炎的方法
公开(公告)号：US20070190030A1
公开(公告)日：2007-08-16
申请号：US11512711
申请日：2006-08-30
申请人： Robert Pawliuk , Philippe Leboulch
发明人： Robert Pawliuk , Philippe Leboulch
IPC分类号： A61K48/00 , C12N15/86
CPC分类号： A61K48/005 , A61K38/1709 , A61K48/00 , A61K48/0075 , C07K14/54 , C12N15/86 , C12N2740/16043 , C12N2740/16045 , C12N2810/6081
摘要： Novel methods for treating and preventing arthritis, such as rheumatoid arthritis, are disclosed which employ lentiviral gene delivery vectors, including HIV-based lentiviral vectors, to deliver a therapeutic gene to a subject. Lentiviral-based vectors treat arthritis by promoting high-level expression of the transferred therapeutic gene in the target tissue of the subject.
摘要翻译：公开了用于治疗和预防关节炎的新方法，例如类风湿性关节炎，其使用包括基于HIV的慢病毒载体的慢病毒基因递送载体将受治疗基因递送至受试者。基于慢病毒的载体通过促进受试者的靶组织中转移的治疗基因的高水平表达来治疗关节炎。

7. 发明授权

US06534314B1 Methods and compositions for transforming cells 有权
标题翻译：用于转化细胞的方法和组合物
公开(公告)号：US06534314B1
公开(公告)日：2003-03-18
申请号：US09293303
申请日：1999-04-16
申请人： Eric Bouhassira , Philippe Leboulch
发明人： Eric Bouhassira , Philippe Leboulch
IPC分类号： C12N1590
CPC分类号： C07K14/805 , C12N15/64 , C12N15/86 , C12N15/90 , C12N2740/13043
摘要： Methods and compositions for transforming cells, resulting in efficient and stable site-specific integration of transgenes, are disclosed. Transformation is achieved by providing an acceptor DNA containing two inverted lox sequences and a donor DNA containing the same two inverted lox sequences, and then contacting the acceptor and donor DNA with a recombinase (e.g., Cre or Flp) which causes recombination at the lox sequences contained in the DNAs. Prior to recombination, the acceptor DNA is preferably integrated into the genome of a cell, such as an embryonic stem cell or a fertilized egg. The acceptor DNA optionally may further contain a negatively selectable marker to allow for screening of cells which have undergone the desired site-specific recombination (e.g., DNA cassette exchange).
摘要翻译：公开了用于转化细胞，导致转基因的有效和稳定的位点特异性整合的方法和组合物。通过提供含有两个反向lox序列的受体DNA和含有相同两个反向lox序列的供体DNA，然后使受体和供体DNA与重组酶（例如Cre或Flp）接触，从而导致lox序列重组，从而实现转化包含在DNA中。在重组之前，受体DNA优选整合到细胞的基因组中，例如胚胎干细胞或受精卵。受体DNA任选地可以进一步含有阴性选择性标记，以允许筛选经历所需位点特异性重组（例如，DNA盒交换）的细胞。

8. 发明申请

US20070072816A1 Methods for inhibiting angiogenesis 审中-公开
标题翻译：抑制血管发生的方法
公开(公告)号：US20070072816A1
公开(公告)日：2007-03-29
申请号：US11403040
申请日：2006-04-11
申请人： Yihai Cao , Renhai Cao , Robert Pawliuk , Philippe Leboulch
发明人： Yihai Cao , Renhai Cao , Robert Pawliuk , Philippe Leboulch
IPC分类号： A61K48/00 , C12N15/09
CPC分类号： A61K38/1866 , A61K38/1858 , C12N2799/027
摘要： Methods for inhibiting angiogenesis using gene therapy are disclosed. Genes encoding PLGF or VEGF-B are delivered to cells e.g., tumor cells, which express VEGF, such that heterodimers of PLGF/VEGF and/or VEGF-B/VEGF are formed within the cells, preferably at a greater ratio than homodimers of VEGF/VEGF. The heterodimers have reduced angiogenic activity compared to VEGF homodimers.
摘要翻译：公开了使用基因治疗抑制血管发生的方法。将编码PLGF或VEGF-B的基因递送至细胞，例如表达VEGF的肿瘤细胞，使得在细胞内形成PLGF / VEGF和/或VEGF-B / VEGF的异二聚体，优选比VEGF的同源二聚体更大的比例 / VEGF。与VEGF同型二聚体相比，异二聚体具有降低的血管生成活性。

9. 发明申请

US20060057725A1 Therapeutic retroviral vectors for gene therapy 有权
标题翻译：用于基因治疗的治疗性逆转录病毒载体
公开(公告)号：US20060057725A1
公开(公告)日：2006-03-16
申请号：US11150785
申请日：2005-06-09
申请人： Philippe Leboulch , Robert Pawliuk , Karen Westerman
发明人： Philippe Leboulch , Robert Pawliuk , Karen Westerman
IPC分类号： C12N15/867
CPC分类号： A61K48/0058 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2227/105 , A01K2267/03 , A61K38/42 , A61K48/00 , C07H21/04 , C07K14/805 , C12N15/63 , C12N15/8509 , C12N15/86 , C12N15/867 , C12N2510/00 , C12N2740/15043 , C12N2740/16011 , C12N2740/16043 , C12N2830/008 , C12N2830/40
摘要： Retroviral gene therapy vectors that are optimized for erythroid specific expression and treatment of hemoglobinopathic conditions are disclosed.
摘要翻译：公开了针对红血球特异性表达和血红蛋白病症状治疗优化的逆转录病毒基因治疗载体。

10. 发明申请

US20150203868A1 GENE THERAPY METHODS 有权
标题翻译：基因治疗方法
公开(公告)号：US20150203868A1
公开(公告)日：2015-07-23
申请号：US14346647
申请日：2011-09-23
申请人： Oliver Negre , Emmanuel Payen , Philippe Leboulch , Yves Beuzard
发明人： Oliver Negre , Emmanuel Payen , Philippe Leboulch , Yves Beuzard
IPC分类号： C12N15/85 , A61K35/28 , A61K35/18 , A61K38/18
CPC分类号： C12N15/85 , A61K35/18 , A61K35/28 , A61K38/1816 , A61K48/00 , A61K2035/124 , C07K14/71 , C07K14/805 , C12N15/86 , C12N2740/16043 , C12N2800/22 , C12N2830/008 , C12N2830/15 , C12N2830/20 , C12N2830/40
摘要： The present invention generally provides improved gene therapy vectors, cell-based compositions, and methods of using the same in methods of gene therapy. The present invention further provides improved gene therapy compositions for expanding hematopoietic cells and related methods for treatment of diseases, disorders, and conditions of the hematopoietic system such as thalassemias and anemias.
摘要翻译：本发明通常提供改进的基因治疗载体，基于细胞的组合物，以及在基因治疗方法中使用它们的方法。本发明进一步提供用于扩增造血细胞的改进的基因治疗组合物和用于治疗诸如地中海贫血和贫血的造血系统的疾病，病症和状况的相关方法。

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