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1. 发明申请

WO2005069762A2 BACILLUS LICHENIFORMIS CHROMOSOME 审中-公开
标题翻译： BACILLUS LICHENIFORMIS染色体
公开(公告)号：WO2005069762A2
公开(公告)日：2005-08-04
申请号：PCT/US2004/037204
申请日：2004-11-05
申请人： NOVOZYMES INC. , NOVOZYMES A/S
发明人： BERKA, Randy , REY, Michael , RAMAIYA, Preethi , ANDERSEN, Jens Tonne , RASMUSSEN, Michael Dolberg , OLSEN, Peter Bjarke
IPC分类号： C07K14/32 , C12Q1/68
CPC分类号： C12Q1/689 , C07K14/32
摘要： The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.
摘要翻译：本发明涉及地衣芽孢杆菌的完整染色体的分离的多核苷酸。本发明还涉及编码生物活性物质的地衣芽孢杆菌染色体和包含该基因的核酸构建体，载体和宿主细胞的分离基因以及用于产生由该基因编码的生物活性物质的方法和使用地衣芽孢杆菌完整染色体的分离基因。

2. 发明申请

WO2008079895A2 METHODS OF OBTAINING GENETIC COMPETENCE IN BACILLUS CELLS 审中-公开
标题翻译：在芽孢杆菌细胞中获得遗传能力的方法
公开(公告)号：WO2008079895A2
公开(公告)日：2008-07-03
申请号：PCT/US2007/088186
申请日：2007-12-19
申请人： NOVOZYMES, INC. , BERKA, Randy , MARANTA, Michelle , TANG, Maria , CHERRY, Barbara
发明人： BERKA, Randy , MARANTA, Michelle , TANG, Maria , CHERRY, Barbara
IPC分类号： C12N15/09
CPC分类号： C12N15/75 , C07K14/32
摘要： The present invention relates to methods of obtaining genetic competence in non-competent Bacillus cells for their transformation with exogenous DNA.
摘要翻译：本发明涉及在非感受态芽孢杆菌细胞中获得用外源DNA转化的遗传能力的方法。

3. 发明申请

WO2008066931A3 BACILLUS LICHENIFORMIS CHROMOSOME 审中-公开
公开(公告)号：WO2008066931A3
公开(公告)日：2008-06-05
申请号：PCT/US2007/024746
申请日：2007-11-29
申请人： NOVOZYMES, INC. , BERKA, Randy , REY, Michael , RAMAIYA, Preethi
发明人： BERKA, Randy , REY, Michael , RAMAIYA, Preethi
IPC分类号： C07K14/32 , C07K14/195 , C12N15/31
摘要： The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis SJ1904 (ATCC PTA-7992). The present invention also relates to isolated polynucleotides of the chromosome of Bacillus licheniformis SJ1904 that encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing biologically active substances encoded by the polynucleotides and to methods of using the isolated polynucleotides of the complete chromosome of Bacillus licheniformis SJ1904.

4. 发明申请

WO2005100582A2 METHODS FOR DEGRADING OR CONVERTING PLANT CELL WALL POLYSACCHARIDES 审中-公开
标题翻译：降解或转化植物细胞壁多糖的方法
公开(公告)号：WO2005100582A2
公开(公告)日：2005-10-27
申请号：PCT/US2005/008325
申请日：2005-03-10
申请人： NOVOZYMES INC.
发明人： BERKA, Randy , CHERRY, Joel
IPC分类号： C12P19/04
CPC分类号： C12P19/14 , C12P5/023 , C12P7/00 , C12P7/02 , C12P7/04 , C12P7/06 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/26 , C12P7/28 , C12P7/40 , C12P13/04 , C12P17/04 , C12P19/02 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microorganisms, and (c) recovering the organic substance from the fermentation.
摘要翻译：本发明涉及用于将植物细胞壁多糖转化成一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖，其中所述重组微生物表达一种或多种异源编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。本发明还涉及有机物质的制造方法，其特征在于，包括：（a）用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因其将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质，和（c）从发酵中回收有机物质。

5. 发明申请

WO2014052630A1 BACTERIAL MUTANTS WITH IMPROVED TRANSFORMATION EFFICIENCY 审中-公开
标题翻译：具有改进效率的细菌菌株
公开(公告)号：WO2014052630A1
公开(公告)日：2014-04-03
申请号：PCT/US2013/061980
申请日：2013-09-26
申请人： NOVOZYMES, INC. , BERKA, Randy , CHERRY, Barbara
发明人： BERKA, Randy , CHERRY, Barbara
IPC分类号： C07K14/32
CPC分类号： C12N15/75 , C07K14/32 , C12P5/007 , C12P5/02 , C12P5/026 , C12P7/04 , C12P13/04 , C12P21/00 , C12P21/02 , Y02E50/343
摘要： Provided herein are Bacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous mecA gene and disruption to an endogenous sinI gene. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.
摘要翻译：本文提供具有改善的转化效率的芽孢杆菌突变体，其包含内源性mecA基因的破坏和内源性sinI基因的破坏。还描述了用于产生突变体的方法，使用突变体产生转化体的方法，以及使用突变体产生多肽或发酵产物的方法。

6. 发明公开

EP3219806A1 METHODS FOR DEGRADING OR CONVERTING PLANT CELL WALL POLYSACCHARIDES 有权
标题翻译：降解或转化植物细胞壁多糖的方法
公开(公告)号：EP3219806A1
公开(公告)日：2017-09-20
申请号：EP17165392.6
申请日：2005-03-10
申请人： Novozymes, Inc.
发明人： BERKA, Randy , CHERRY, Joel
IPC分类号： C12P19/04 , C12P7/06
摘要： The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.
摘要翻译：本发明涉及将植物细胞壁多糖转化为一种或多种产物的方法，其包括：用有效量的重组微生物的全废发酵液处理植物细胞壁多糖，其中所述重组微生物表达一种或多种异源编码将植物细胞壁多糖降解或转化为一种或多种产物的酶的基因。本发明还涉及生产有机物质的方法，其包括：（a）用有效量的重组微生物的全废发酵液糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因其将植物细胞壁多糖降解或转化成糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化材料; 和（c）从发酵中回收有机物质。

7. 发明授权

EP0871746B1 CARBOXYPEPTIDASES FROM ASPERGILLUS ORYZAE AND NUCLEIC ACIDS ENCODING SAME 失效
标题翻译：羧肽酶米曲霉和编码这些核酸
公开(公告)号：EP0871746B1
公开(公告)日：2005-11-16
申请号：EP97944627.5
申请日：1997-10-03
申请人： Novozymes, Inc. , Novozymes A/S
发明人： BLINKOVSKY, Alexander , BERKA, Randy , REY, Michael , GOLIGHTLY, Elizabeth , KLOTZ, Alan , MATHISEN, Thomas, Erik , DAMBMANN, Claus , BROWN, Kimberly
IPC分类号： C12N15/57 , C12N15/70 , C12N9/48 , C12N1/21 , A23J3/34
CPC分类号： C12N9/48 , A23J3/341 , A23J3/346 , A23L27/24
摘要： The present invention relates to polypeptides having carboxypeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to methods of obtaining protein hydrolysates useful as flavor improving agents.

8. 发明授权

EP2900689B1 BACTERIAL MUTANTS WITH IMPROVED TRANSFORMATION EFFICIENCY 有权
标题翻译：提高转化效率的细菌突变体
公开(公告)号：EP2900689B1
公开(公告)日：2017-08-02
申请号：EP13773567.6
申请日：2013-09-26
申请人： Novozymes, Inc.
发明人： BERKA, Randy , CHERRY, Barbara
IPC分类号： C07K14/32 , C12P21/00 , C12P21/02 , C12N15/75 , C12P5/00 , C12P5/02 , C12P7/04 , C12P13/04
CPC分类号： C12N15/75 , C07K14/32 , C12P5/007 , C12P5/02 , C12P5/026 , C12P7/04 , C12P13/04 , C12P21/00 , C12P21/02 , Y02E50/343

9. 发明授权

EP1735454B1 METHODS FOR DEGRADING OR CONVERTING PLANT CELL WALL POLYSACCHARIDES 有权
标题翻译： VERFAHREN ZUM ABBAU ODER ZUR UMWANDLUNG PFLANZLICHER ZELLWANDPOLYSACCHARIDE
公开(公告)号：EP1735454B1
公开(公告)日：2017-05-10
申请号：EP05736399.6
申请日：2005-03-10
申请人： Novozymes, Inc.
发明人： BERKA, Randy , CHERRY, Joel
IPC分类号： C12P19/04 , C12P7/06
CPC分类号： C12P19/14 , C12P5/023 , C12P7/00 , C12P7/02 , C12P7/04 , C12P7/06 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/26 , C12P7/28 , C12P7/40 , C12P13/04 , C12P17/04 , C12P19/02 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.
摘要翻译：本发明涉及将植物细胞壁多糖转化为一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全部发酵液对植物细胞壁多糖进行处理，其中重组微生物表达一种或多种异源编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。本发明还涉及有机物质的制造方法，其特征在于，包括：（a）将有效量的重组微生物用的全部发酵液对植物细胞壁多糖进行糖化，其中所述重组微生物表达编码酶的一种或多种异源基因将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质; 和（c）从发酵中回收有机物质。

10. 发明公开

EP2900689A1 BACTERIAL MUTANTS WITH IMPROVED TRANSFORMATION EFFICIENCY 有权
标题翻译：香肠麻醉剂麻醉机变压器变压器
公开(公告)号：EP2900689A1
公开(公告)日：2015-08-05
申请号：EP13773567.6
申请日：2013-09-26
申请人： Novozymes, Inc.
发明人： BERKA, Randy , CHERRY, Barbara
IPC分类号： C07K14/32
CPC分类号： C12N15/75 , C07K14/32 , C12P5/007 , C12P5/02 , C12P5/026 , C12P7/04 , C12P13/04 , C12P21/00 , C12P21/02 , Y02E50/343
摘要： Provided herein are Bacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous mecA gene and disruption to an endogenous sinI gene. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.
摘要翻译：本文提供了具有改善的转化效率的芽孢杆菌突变体，包括内源性mecA基因的破坏和内源性sinI基因的破坏。还描述了用于产生突变体的方法，使用突变体产生转化体的方法，以及使用突变体产生多肽或发酵产物的方法。

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