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1. 发明申请

WO2011117396A3 THERMOSTABLE PHYTASE VARIANTS 审中-公开
标题翻译：热稳定的植物酶变体
公开(公告)号：WO2011117396A3
公开(公告)日：2011-11-24
申请号：PCT/EP2011054639
申请日：2011-03-25
申请人： NOVOZYMES AS , SKOV LARS KOBBEROEE , MARIA LEONARDO DE , MATSUI TOMOKO
发明人： SKOV LARS KOBBEROEE , DE MARIA LEONARDO , MATSUI TOMOKO
IPC分类号： C12N9/16 , A01H5/00 , C12N15/55 , C12N15/82
CPC分类号： C12N9/96 , A23K10/16 , A23L33/195 , A61K38/46 , C12N9/16 , C12N15/8243 , C12P7/00 , C12P7/14 , C12P21/00 , C12Y301/03008 , C12Y301/03026 , C12Y301/03072
摘要： The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
摘要翻译：本发明涉及产生与来自布氏柠檬酸杆菌的肌醇六磷酸酶具有至少74％同一性并且与该植酸酶相比包含至少两个另外的二硫键的肌醇六磷酸酶变体的方法。这些肌醇六磷酸酶变体具有修饰的，优选改进的性质，如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。本发明还涉及所产生的变体，编码这些肌醇六磷酸酶的DNA，它们的生产方法以及它们的用途，例如，在动物饲料和动物饲料添加剂中。

2. 发明申请

WO2008017646A3 EXPRESSION OF GENES FROM GRAM NEGATIVE BACTERIA IN FUNGI 审中-公开
标题翻译：格兰氏阴性细菌在真菌中的表达
公开(公告)号：WO2008017646A3
公开(公告)日：2008-06-26
申请号：PCT/EP2007058077
申请日：2007-08-03
申请人： NOVOZYMES AS , MATSUI TOMOKO , JOHANNESEN PIA FRANCKE , TAKAGI SHINOBU , POULSEN THOMAS AGERSTEN , TSUTSUMI NORIKO , LIU ZHENG , KONG XIANGYU
发明人： MATSUI TOMOKO , JOHANNESEN PIA FRANCKE , TAKAGI SHINOBU , POULSEN THOMAS AGERSTEN , TSUTSUMI NORIKO , LIU ZHENG , KONG XIANGYU
IPC分类号： C12N9/16 , C12N1/15 , C12N15/55 , C12N15/62 , C12N15/80 , C12P21/02
CPC分类号： C12N9/16 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.
摘要翻译：本发明提供了一种在适于工业生产的真菌宿主中重组表达源自革兰氏阴性细菌的多肽的方法。在第一方面，本发明涉及用于在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，所述方法包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一编码真菌信号肽的核酸序列和编码所述多肽的第二核酸序列，其具有至少一个修饰的密码子，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与存在于所述革兰氏阴性细菌中的野生型核酸序列中的相应密码子; ii）在真菌宿主中表达修饰的核酸序列。

3. 发明申请

WO2013006756A3 ALPHA AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译： ALPHA AMYLASE变体和编码它的多核苷酸
公开(公告)号：WO2013006756A3
公开(公告)日：2013-05-02
申请号：PCT/US2012045670
申请日：2012-07-06
申请人： NOVOZYMES AS , NOVOZYMES NORTH AMERICA INC , MATSUI TOMOKO , TOMIKI AKI , COWARD-KELLY GUILLERMO
发明人： MATSUI TOMOKO , TOMIKI AKI , COWARD-KELLY GUILLERMO
IPC分类号： C12N15/56 , C12N9/24 , C12P19/14
CPC分类号： C12N9/2417 , C07K2319/20 , C12N9/242 , C12P7/06 , C12P7/10 , C12P19/02 , C12P19/14 , C12P19/20 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
摘要翻译：本发明涉及α淀粉酶的变体。本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

4. 发明申请

WO2011072191A3 PROTEASE VARIANTS 审中-公开
公开(公告)号：WO2011072191A3
公开(公告)日：2012-04-19
申请号：PCT/US2010059814
申请日：2010-12-10
申请人： NOVOZYMES AS , NOVOZYMES NORTH AMERICA INC , MATSUI TOMOKO , NOERGAARD ALLAN , POULSEN THOMAS AGERSTEN , MATTHEWS JOHN
发明人： MATSUI TOMOKO , NOERGAARD ALLAN , POULSEN THOMAS AGERSTEN , MATTHEWS JOHN
IPC分类号： C12N9/58
CPC分类号： C12N9/58 , C12P7/06 , C12Y304/24 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to proteases having at least 75% identity to a protease derived from Thermoascus aurantiacus and comprises at least one modification in the amino acid sequence threrof. These protease variants have improved thermostability. The invention also relates to DNA encoding these proteases, methods of their production, as well as the use thereof.
摘要翻译：本发明涉及蛋白酶，其与衍生自嗜热from鱼的蛋白酶具有至少75％的同一性，并且包含氨基酸序列threrof中的至少一个修饰。这些蛋白酶变体具有改善的热稳定性。本发明还涉及编码这些蛋白酶的DNA，其生产方法及其用途。

5. 发明申请

WO2005003311A8 ENZYMES FOR STARCH PROCESSING 审中-公开
标题翻译：淀粉加工酶
公开(公告)号：WO2005003311A8
公开(公告)日：2006-02-09
申请号：PCT/US2004020499
申请日：2004-06-25
申请人： NOVOZYMES AS , NOVOZYMES NORTH AMERICA INC , TAIRA RIKAKO , TAKAGI SHINOBU , HJORT CARSTEN , VIKSO-NIELSEN ANDERS , ALLAIN ERIC , UDAGAWA HIROAKI , FUKUYAMA SHIRO , MATSUI TOMOKO
发明人： TAIRA RIKAKO , TAKAGI SHINOBU , HJORT CARSTEN , VIKSO-NIELSEN ANDERS , ALLAIN ERIC , UDAGAWA HIROAKI , FUKUYAMA SHIRO , MATSUI TOMOKO
IPC分类号： C12N9/26 , C12N9/30 , C12N9/34 , C12N15/56
CPC分类号： C12N9/242 , C12N9/2414 , C12N9/2425 , C12N9/2428
摘要： The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction

6. 发明申请

WO2005003311A3 ENZYMES FOR STARCH PROCESSING 审中-公开
标题翻译：淀粉加工的酶
公开(公告)号：WO2005003311A3
公开(公告)日：2009-02-19
申请号：PCT/US2004020499
申请日：2004-06-25
申请人： NOVOZYMES AS , NOVOZYMES NORTH AMERICA INC , TAIRA RIKAKO , TAKAGI SHINOBU , HJORT CARSTEN , VIKSO-NIELSEN ANDERS , ALLAIN ERIC , UDAGAWA HIROAKI , FUKUYAMA SHIRO , MATSUI TOMOKO
发明人： TAIRA RIKAKO , TAKAGI SHINOBU , HJORT CARSTEN , VIKSO-NIELSEN ANDERS , ALLAIN ERIC , UDAGAWA HIROAKI , FUKUYAMA SHIRO , MATSUI TOMOKO
IPC分类号： G01N33/53 , C12N9/26 , C12N9/30 , C12N9/34
CPC分类号： C12N9/242 , C12N9/2414 , C12N9/2425 , C12N9/2428
摘要： The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction
摘要翻译：本发明涉及包含碳水化合物结合模块氨基酸序列和真菌α-淀粉酶氨基酸序列的杂合酶以及包含碳水化合物结合模块和α-淀粉酶催化模块的真菌野生型酶变体。本发明还涉及杂交酶或变体在淀粉液化中的用途

7. 发明申请

WO2006069290A3 ENZYMES FOR STARCH PROCESSING 审中-公开
标题翻译：淀粉加工酶
公开(公告)号：WO2006069290A3
公开(公告)日：2007-05-31
申请号：PCT/US2005046725
申请日：2005-12-22
申请人： NOVOZYMES AS , NOVOZYMES NORTH AMERICA INC , FUKUYAMA SHIRO , MATSUI TOMOKO , SOONG CHEE-LEONG , ALLAIN ERIC , VIKSO-NIELSEN ANDERS , UDAGAWA HIROAKI , LIU YE , DUAN JUNXIN , WU WENPING , ANDERSEN LENE NONBOE , LANDVIK SARA
发明人： FUKUYAMA SHIRO , MATSUI TOMOKO , SOONG CHEE-LEONG , ALLAIN ERIC , VIKSO-NIELSEN ANDERS , UDAGAWA HIROAKI , LIU YE , DUAN JUNXIN , WU WENPING , ANDERSEN LENE NONBOE , LANDVIK SARA
IPC分类号： C12N9/32 , C07H21/04 , C12N1/16 , C12N15/74
CPC分类号： C12P19/14 , C07K2319/00 , C12N9/242 , C12N9/2428 , C12P7/06 , C12P19/02 , C12Y302/01001 , C12Y302/01003 , Y02E50/17
摘要： The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.
摘要翻译：本发明涉及包含碳水化合物结合组件氨基酸序列和α-淀粉酶氨基酸序列以及这些多肽的应用的多肽。

8. 发明申请

WO2012088303A2 PROCESSES FOR PRODUCING FERMENTATION PRODUCTS 审中-公开
标题翻译：生产发酵产品的方法
公开(公告)号：WO2012088303A2
公开(公告)日：2012-06-28
申请号：PCT/US2011066559
申请日：2011-12-21
申请人： NOVOZYMES NORTH AMERICA INC , NOVOZYMES AS , DEINHAMMER RANDY , CLARK SUZANNE , QUIROS MAURICIO , MATTHEWS JOHN , HJULMAND ANNE GLUD , SOONG CHEE-LEONG , MATSUI TOMOKO , TAKAGI SHINOBU
发明人： DEINHAMMER RANDY , CLARK SUZANNE , QUIROS MAURICIO , MATTHEWS JOHN , HJULMAND ANNE GLUD , SOONG CHEE-LEONG , MATSUI TOMOKO , TAKAGI SHINOBU
IPC分类号： A61B7/02
CPC分类号： C12P7/06 , C12P19/02 , C12P19/14 , C12P19/20 , C12Y302/01001 , C12Y302/01003 , C12Y304/24 , Y02E50/17
摘要： The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.
摘要翻译：本发明涉及用于从含淀粉材料生产发酵产物的方法，其中在液化期间存在和/或加入热稳定性α-淀粉酶和任选的热稳定蛋白酶。本发明还涉及适用于本发明方法的组合物。

9. 发明申请

WO2010034835A3 HAFNIA PHYTASE VARIANTS 审中-公开
公开(公告)号：WO2010034835A3
公开(公告)日：2010-06-24
申请号：PCT/EP2009062539
申请日：2009-09-28
申请人： NOVOZYMES AS
发明人： LASSEN SOEREN FLENSTED , SKOV LARS KOBBEROEE , DE MARIA LEONARDO , FRIIS ESBEN PETER , MATSUI TOMOKO , NOERGAARD ALLAN , VIND JESPER
IPC分类号： C12N9/16 , A23K1/165
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensitiliby, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed(such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of thei r production, as well as the use thereof, e.g. in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有修饰的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面具有改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介; 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如，在动物饲料和动物饲料添加剂中。

10. 发明申请

WO2015110473A3 PULLULANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译： PULLULANASE变体和编码它的多核苷酸
公开(公告)号：WO2015110473A3
公开(公告)日：2015-09-17
申请号：PCT/EP2015051145
申请日：2015-01-21
申请人： NOVOZYMES AS
发明人： MATSUI TOMOKO , YAMAGISHI AKIHIKO
IPC分类号： C12N9/44 , A23L1/09 , C12P19/16
CPC分类号： C12N9/2457 , C12P7/06 , C12P19/02 , C12P19/16 , C12P2203/00 , C12Y302/01041 , Y02E50/17
摘要： The present invention relates to pullulanase variants having increased thermoactivity and comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The variants are mainly derived from pullulanases from Bacillus deramificans, Bacillus acidopullulyticus or hybrid pullulanases.
摘要翻译：本发明涉及具有增加的热活性的支链淀粉酶变体，并且包括在对应于位置393,143,150,243,244,345,346,368,370,373,381,368,385,337,381,382,385的一个或多个位置上的母体支链淀粉酶的取代，387,402,49,430,431,432,456,468,492,610,624,626,632,665和699的多核苷酸。本发明还涉及编码变体的多核苷酸 ; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。变异体主要衍生自来自芽孢杆菌属（Bacillus deramificans），Bacillus acidulullulyticus或杂合支链淀粉酶的支链淀粉酶。

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