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1. 发明申请

WO0132829A3 METHODS TO SCREEN MICROORGANISMS OR GENE LIBRARIES FOR PRODUCTS SECRETED FROM A CELL 审中-公开
标题翻译：筛选从细胞分泌的产物的微生物或基因库的方法
公开(公告)号：WO0132829A3
公开(公告)日：2001-12-13
申请号：PCT/DK0000566
申请日：2000-10-10
申请人： NOVOZYMES AS
发明人： MOELLER SOEREN , KONGSBAK LARS , KRISTENSEN HANS-HENRIK , VIND JESPER , PEDERSEN HENRIK , HUSUM TOMMY LYKKE
IPC分类号： C12N15/10
CPC分类号： C12N15/1034
摘要： The invention describes methods for screening for products secreted from the cells, and provides methods to establish a correlation between the activity of the secreted product and the secreting cell. Accordingly in a first aspect the present invention relates to a method for screening a DNA library for DNA of interest comprising the steps of a) creating host cells comprising the DNA library, b) generating samples each comprising a host cell of step a), c) establishing a means for correlating host cell secretion of a material of interest in a sample with the fluorescence of the sample, d) determining which intensity interval of fluorescence indicates secretion in the sample when the correlating means of step c) is used, e) cultivating the samples under suitable conditions, and f) selecting the samples exhibiting fluorescence within the intensity interval of step d) using a fluorescence analyzer; wherein the host cell comprises DNA of interest.
摘要翻译：本发明描述了筛选从细胞分泌的产物的方法，并提供了确定分泌产物的活性与分泌细胞之间的相关性的方法。因此，在第一方面，本发明涉及用于筛选目标DNA的DNA文库的方法，包括以下步骤：a）产生包含DNA文库的宿主细胞，b）产生各自包含步骤a），c ）建立用于将样品中感兴趣的材料的宿主细胞分泌与样品的荧光相关的手段，d）确定荧光的强度间隔表示分泌的浓度使用步骤c）的相关手段进行样品，e）在合适的条件下培养样品，以及f）使用荧光分析仪选择在步骤d）的强度间隔内显示荧光的样品; 其中所述宿主细胞包含感兴趣的DNA。

2. 发明专利

CA2374347A1 SCREENING METHOD FOR PEPTIDES 未知
公开(公告)号：CA2374347A1
公开(公告)日：2000-12-07
申请号：CA2374347
申请日：2000-05-29
申请人： NOVOZYMES AS
发明人： KRISTENSEN HANS-HENRIK
IPC分类号： C12N15/09 , A61K38/00 , A61K38/43 , A61K48/00 , A61P31/04 , A61P35/00 , C12N15/10 , C07K14/00 , C12N15/74 , C12N15/80
摘要： The present invention relates to a method for screening nucleotide sequences encoding anti microbial peptides comprising the steps of: a) ligating a plasmid with the pool of nucleotide sequences operably linked to an inducibl e promoter, so as to express a peptide which is an enzyme or a mature peptide of less than 100 amino acid residues, optionally linked to a signal peptide, b) transforming host cells which are sensitive to the peptide with the ligated plasmids, c) screening the transformed host cells so as to select viable cells, d) cultivating the viable cells in the presence of inducer so as to induce expressions of said nucleotide sequence, e) selecting cells according to the effect of the inducer on cell growth, and recovering the nucleotide sequence encoding the peptide from the selected cells. DNA shuffling is emphasized as a method for producing the pool of nucleotides.

3. 发明专利

AU5208400A Screening method for peptides 未知
公开(公告)号：AU5208400A
公开(公告)日：2000-12-18
申请号：AU5208400
申请日：2000-05-29
申请人： NOVOZYMES AS
发明人： KRISTENSEN HANS-HENRIK
IPC分类号： C12N15/09 , A61K38/00 , A61K38/43 , A61K48/00 , A61P31/04 , A61P35/00 , C12N15/10 , C07K14/00 , C12N15/74 , C12N15/80

4. 发明专利

AU2005209649B2 Screening method for peptides 未知
公开(公告)号：AU2005209649B2
公开(公告)日：2008-05-29
申请号：AU2005209649
申请日：2005-09-09
申请人： NOVOZYMES AS
发明人： KRISTENSEN HANS-HENRIK
IPC分类号： C12N15/10 , C07K14/00 , C12N15/74 , C12N15/80

5. 发明专利

AU2005209649A1 Screening method for peptides 未知
公开(公告)号：AU2005209649A1
公开(公告)日：2005-10-06
申请号：AU2005209649
申请日：2005-09-09
申请人： NOVOZYMES AS
发明人： KRISTENSEN HANS-HENRIK
IPC分类号： C07K14/00 , C12N15/10 , C12N15/74 , C12N15/80

6. 发明专利

AU7646000A Methods to screen microorganisms or gene libraries for products secreted from a cell 未知
公开(公告)号：AU7646000A
公开(公告)日：2001-05-14
申请号：AU7646000
申请日：2000-10-10
申请人： NOVOZYMES AS
发明人： MOELLER SOEREN , KONGSBAK LARS , KRISTENSEN HANS-HENRIK , VIND JESPER , PEDERSEN HENRIK , HUSUM TOMMY LYKKE
IPC分类号： C12N15/10

7. 发明专利

AU7645900A A method of screening cell populations 未知
公开(公告)号：AU7645900A
公开(公告)日：2001-05-14
申请号：AU7645900
申请日：2000-10-10
申请人： NOVOZYMES AS
发明人： PEDERSEN HENRIK , KRISTENSEN HANS-HENRIK , VIND JESPER , HANSEN PETER KAMP , LAMSA MICHAEL , NOERREGAARD-MADSEN MADS
IPC分类号： C12N15/10 , C40B40/02 , C12N1/00

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